Deneysel Ülseratif Kolit Üzerine Yüksek Karbonhidratlı, Yüksek Yağlı ve Aralıklı Beslemenin Etkisi

Yıl 2018, Cilt: 7 Sayı: 1, 7 - 14, 04.07.2018

Ahmet Uyar , Hüseyin Emlik Turan Yaman

https://doi.org/10.31196/huvfd.458770

Cited By: 2

Öz

Bu
çalışmada, dünyada görülme sıklığı artan inflamatuvar bağırsak hastalıklarının
(İBH) bir tanesi olan Ülseratif kolit (ÜK) hastalığının Wistar albino türü
ratlarda deneysel oluşumu üzerine beslenme şekli ve sıklığının karşılaştırmalı
olarak araştırılması amaçlanmıştır. Bu amaçla; Kontrol (n=7), Kolit (n=7),
Kolit-Yüksek Karbonhidratlı Diyet (K-YKD) (n=7), Kolit–Yüksek Karbonhidratlı
Aralıklı Diyet (K-YKAD) (n=7), Kolit–Yüksek Yağlı Diyet (K-YYD) (n=7), Kolit-Yüksek
Yağlı Aralıklı Diyet (K-YYAD) (n=7) olacak şekilde çalışma grupları
oluşturuldu. Gruplardan aralıklı besleme gruplarına haftada sadece 2 gün (ardarda
olmayan) diyet verilmesine 24 saat ara verildi. 7 haftalık beslemeden sonra deneysel
kolit modeli; kolit ve tedavi gruplarındaki (Kolit, K-YKD, K-YKAD, K-YYD,
K-YYAD) ratlara anestezi altında 30^o trendelenburg pozisyonunda 8
mm’lik kateterin rektal yoldan 8 cm ileriye asetik asidin (pH 2.4, % 4)
intrarektal (i.r.) olarak uygulanması ile oluşturuldu. 72 saat sonra ratlar
sakrifiye edilerek histopatolojik örnekler %10 tamponlu formaldehite alındı. Histopatolojik
incelemelere göre asetik asitin ciddi kolit hasarlarına neden olduğu buna
karşın aralıklı olarak verilen diyet gruplarında bu hasarların oldukça minimal
düzeyde kaldığı görülmüştür. Sonuç olarak kolit modelinde aralıklı diyet
uygulamasının olumlu etkileri olduğu kanısına varıldı. 

Anahtar Kelimeler

Yüksek Karbonhidratlı, Yağlı ve Aralıklı Diyet, Kolit, Rat

Effect of High Carbohydrate, Fat and Intermittent Fasting Diet on Experimental Ulcerative Colitis

Öz

In this study, it was aimed to comparatively investigate the effect of  diet type and feeding frequency on occurence of experimental ulcerative colitis (UC), in Wistar albino rats. For this purpose, 7 rats were randomly selected and 6 groups were formed as Control (n=7), Colitis (n=7), Colitis-High Carbohydrate Diet (C-HCD) (n=7), Colitis - High Carbohydrate Diet with Intermittent Feeding (C-HCIFD) (n=7), Colitis -High Fat Diet (C-HFD) (n=7), Colitis-High Fat Diet with Intermittent Feeding (C-HFIFD) (n=7). In intermittent feeding groups the feeding  was interrupted twice in a week for 24 hours . After 7 weeks of feeding, experimental colitis model was induced in colitis and treatment groups rats by intrarectal administration of acetic acid (pH 2.4, 4 %). After the 72 hours rats were sacrificed and colon samples were taken into 10% formalin solution. Histopathological studies have shown that acetic acid caused severe colitis damage, whereas the damage remained at minimal levels in groups with intermittent feeding. As a result, it was concluded that intermittent feeding administration in the colitis model had positive effects.

Anahtar Kelimeler

High Carbohydrate, Fat and Intermittent Fasting Diet, Colitis, Rat

Kaynakça

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