Babesiosis’li köpeklerde tedavi öncesi ve sonrası haptoglobin, seruloplazmin ve bazı biyokimyasal parametrelerin seviyelerinin belirlenmesi

Yıl 2019, Cilt: 8 Sayı: 1, 77 - 80, 01.07.2019

Ekin Emre Erkılıç

https://doi.org/10.31196/huvfd.592564

Cited By: 1

Öz

Bu çalışmada babesiosis ile doğal enfekte köpeklerde
tedavi öncesi ve sonrası haptoglobin (Hp), seruloplazmin (Cp) ve bazı
biyokimyasal parametrelerin seviyelerinin belirlenmesi amaçlanmıştır.
Çalışmanın hayvan materyalini, iştahsızlık, ikterus ve hemoglobinüri
şikayetleri olan farklı yaş ve cinsiyette 10 köpek oluşturdu. Klinik muayeneler
ve Giemsa boyama sonrasında eritrositler içerisinde etkenlerin görülmesi ile
tanı kondu. Tanı sonrasında tedavi öncesi ve sonrası hayvanlardan kan alınarak
serumları ayrıştırıldı. Bu serumlarda Alanin aminotransferaz (ALT) ve Aspartat
aminotransferaz (AST) enzim aktiviteleri, Hp, Cp, üre ve kreatinin seviyeleri
belirlendi. Tedavi öncesi ALT (P<0.05) ve AST (P<0.05) enzim aktiviteleri
ile üre (P<0.05), kreatinin (P<0.05) ve Cp (P<0.001), seviyeleri
tedavi sonrasına göre yüksek belirlendi. Hp seviyesi ise tedavi öncesinde
tedavi sonrasına göre düşük bulundu (P<0.001). Sonuç olarak Babesiosis’li
köpeklerde ALT ve AST enzim aktiviteleri ile üre, kreatinin, Hp ve Cp
seviyelerinde önemli değişikliklerin olduğu belirlendi. Köpeklerde babesiosis
hastalığının prognozunda ve tedavi sürecinde bu parametrelerin takibinin önemli
olabileceği kanaatine varıldı. 

Anahtar Kelimeler

Köpek, Babesiosis, Haptoglobin, Seruloplazmin

Teşekkür

Çalışmanın biyokimyasal analizlerin yapılmasında destek olan Doç. Dr. Oğuz MERHAN’a yardımlarından dolayı teşekkür ederim.

Determination of haptoglobin, ceruloplasmin and some biochemical parameters before and after treatment in dogs with babesiosis

Öz

In this study, it was aimed
to determine haptoglobin (Hp), ceruloplasmin (Cp) and some biochemical
parameters levels before and after treatment in dogs naturally infected with
babesiosis. Animal material of the study consisted of 10 dogs of different age
and sex. There were icterus, hemoglobinuria and lack of appetite in dogs. After
clinical examination and Giemsa staining, disease was diagnosed by the presence
of agents in erythrocytes. After diagnosis, blood samples were taken from
animals before and after treatment and sera was separated. In these sera,
Alanine Aminotransferase (ALT) and Aspartate Aminotransferase (AST) enzyme
activities, Hp, Cp, urea and creatinine levels were determined. Pre-treatment
ALT (P<0.05) and AST (P<0.05) enzyme activities, urea (P<0.05),
creatinine (P<0.05) and Cp (P<0.001) levels were determined higher than
post-treatment. Pre-treatment Hp level was determined lower compared to
post-treatment level (P<0.001). As a result, significant changes in ALT and
AST enzyme activities, urea, creatinine, Hp and Cp levels were determined in
dogs with Babesiosis. It was concluded that follow-up of these parameters may
be important in the prognosis and treatment process of the disease in dogs.

Anahtar Kelimeler

Dog, Babesiosis, Haptoglobin, Ceruloplasmin

Kaynakça

• Aysul N, Ural K, Ulutaş B, Eren H, Karagenç T, 2013: First detection and molecular identification of Babesia gibsoni in two dogs from the Aydın province of Turkey. Turk J Vet Anim Sci, 37, 226-229.
• Baneth G, 2018: Antiprotozoal treatment of canine babesiosis. Vet Parasitol, 254, 58-63.
• Batchelor J, Fuller J, Woodman DD, 1989: A simple method for measurement of the haemoglobin binding capacity of canine haptoglobin. Lab Anim, 23, 365-369.
• Ceron JJ, Eckersall PD, Martýnez-Subiela S, 2005: Acute phase proteins in dogs and cats: current knowledge and future perspectives. Vet Clin Pathol, 34, 85-99.
• Colombo JP, Richterich R, 1964: Zur bestimmung des caeruloplasmin im plasma [on the determination of ceruloplasmin in plasma]. Schweiz Med Wochenschr, 94, 715-720.
• Cray C, 2012: Acute phase proteins in animals. Prog Mol Biol Transl Sci, 105, 113-150.
• Çınar M, Erat S, Arıkan Ş, Mamak N, Oğrak YZ, Güzel M, 2010: Kangal köpeklerinde bazı biyokimyasal parametreler üzerine yaş ve cinsiyetin etkisi. Erciyes Üniv Vet Fak Derg, 7, 109-116.
• dos Santos Schmidt EM, Rubio CP, dos Santos GJ, Barbosa L, da Motta Santos TF, de Cezaro MC, 2015: Serum protein profile of hookworm infection in dogs. Comp Clin Pathol, 24, 1463-1466.
• Eichenberger RM, Riond B, Willi B, Hofmann-Lehmann R, Deplazes P, 2016: Prognostic markers in acute babesia canis ınfections. J Vet Intern Med, 30, 174-182.
• Furlanello T, Fiorio F, Caldin M, Lubas G, Solano-Gallego L, 2005: Clinicopathological findings in naturally occurring cases of babesiosis caused by large form Babesia from dogs of northeastern Italy. Vet Parasitol, 134, 77-85.
• Gonde S, Chhabra S, Sıngla LD, Randhawa CS, 2017: Clinico-Haemato-Biochemıcal changes in naturally occurring canine babesiosis in Punjab, Indıa. Malaysian J Vet Res, 8, 37-44.
• Gökçe E, Kırmızıgül AH, Taşçı GT, Uzlu E, Gündüz N, Vatansever Z, 2013: Türkiye’de köpeklerde Babesia canis canis’in klinik ve parazitolojik olarak ilk tespiti. Kafkas Univ Vet Fak Derg, 19, 717-720.
• Kocaturk M, Martinez S, Eralp O, Tvarijonaviciute A, Ceron J, Yilmaz Z, 2010: Prognostic value of serum acute-phase proteins in dogs with parvoviral enteritis. J Small Anim Pract, 51, 478-483.
• McGrotty YL, Knottenbelt CM, Ramsey IK, Reid SWJ, Eckersall PD, 2003: Haptoglobin concentrations in a canine hospital population. Vet Rec, 152, 562-564.
• Murata H, Shimada N, Yoshioka M, 2004: Current research on acute phase proteins in veterinary diagnosis: an overview. Vet J, 168, 28-40.
• Shah SA, Sood NK, Tumati SR, 2011: Haemato-biochemical changes in natural cases of canine babesiosis. Asian J Anim Sci, 5, 387-392.
• Solano-Gallego L, Baneth G, 2011: Babesiosis in dogs and cats-expanding parasitological and clinical spectra. Vet Parasitol, 181, 48-60.
• SPSS 18: SPSS Inc., Version 18, Chicago, USA.
• Sudhakara Reddy B, Sivajothi S, Varaprasad Reddy LS, Solmon Raju KG, 2016: Clinical and laboratory findings of Babesia infection in dogs. J Parasit Dis, 40, 268-272.
• Tuna GE, Ulutaş B, 2015: Hastalıkların biyobelirteçleri olarak akut faz proteinleri. Turkiye Klinikleri J Vet Sci Intern Med-Special Topics, 1, 8-19.
• Ulutaş B, Bayramli G, Ulutaş PA, Karagenc T, 2005: Serum concentration of some acute phase proteins in naturally occurring canine babesiosis: a preliminary study. Vet Clin Pathol, 34, 144-147.
• Ulutaş PA, Ulutaş B, Sarıerler M, Bayramlı G, 2007: Serum haptoglobin and ceruloplasmin concentrations in dogs with various diseases. İstanbul Üniv Vet Fak Derg, 33, 35-42.
• Zamokas G, Grigonis A, Karvelienė B, Daunoras G, Babickaitė L, Šapalienė I, 2014: Importance of haematological changes in diagnosing canine babesiosis. Vet Med Zoot, 67, 94-98.