Dimetil Benzantrasen Uygulanan Ratlarda Tunceli Dağ Sarımsağı (Allium tuncelianum) ve Vitamin E‘nin Bağırsak Dokusundaki Lipit Peroksidasyonu ve Antioksidan Enzim Aktivitelerine Etkisi

Yıl 2019, Cilt: 8 Sayı: 2, 191 - 197, 25.12.2019

Kasım Takım Türkan Kutlu

https://doi.org/10.31196/huvfd.667738

Cited By: 2

Öz

Bu çalışmada; 7,12-dimethyl benzanthracene (DMBA) ile indüklenen ratlara Tunceli Dağ Sarımsağı (Allium tuncelianum) ve vitamin E uygulamasının, rat bağırsak dokusundaki lipit peroksidasyon ve antioksidan enzim aktivitelerine etkisinin belirlenmesi amaçlandı. Çalışmada, Wistar albino tipi dişi ratlar kullanıldı. Her grupta 8 adet (n=8) hayvan olmak üzere, 5 grup oluşturuldu. 1.Grup; Kontrol grubu, 2.Grup; 7,12-dimethyl benzanthracene (DMBA) verilen grup, 3.Grup; 7.12-DMBA’ya ilaveten 250 mg/kg/gün Tunceli Dağ Sarımsağı (TDS) verilen grup, 4.Grup; 7.12-DMBA’ya ilaveten 500 mg/kg/gün TDS verilen grup ve 5.Grup; 7.12-DMBA’ya ilaveten 200 mg/kg (haftada iki kez) E vitamini verilen gruptu. 30 günlük deneme sonunda; ratlara ötenazi uygulandı ve bağırsak dokusu çıkarılarak homojenize edildi. Bağırsak doku homojenatındaki lipit peroksidasyon ve antioksidan enzim düzeyi spektrofotometrik yöntemle ölçüldü. Çalışma sonucunda DMBA uygulanan grubun (Grup 2) bağırsak dokusunda: katalaz (CAT), total glutatyon (GSH) ve süperoksit dismutaz (SOD) enzim aktivitesinin kontrol grubuna göre anlamlı olarak azaldığı (P<0.05), MDA düzeyinin ise anlamlı olarak arttığı (P<0.05) belirlendi. DMBA ile birlikte TDS'nın düşük dozunun uygulandığı grupta (Grup 3) CAT ve SOD enzim düzeylerinde kontrol grubuna göre anlamlı artış (P<0.05) belirlendi. Bu grup, vitamin E verilen grupla kıyaslandığında ise; CAT ve SOD düzeylerinde anlamlı artış (P<0.05), GSH ve MDA düzeylerinde ise anlamlı azalma (P<0.05) saptandı. DMBA ile birlikte TDS'nın yüksek dozunun uygulandığı grupta (Grup 4); CAT ve SOD enzim düzeylerinde, hasta grupla kıyaslandığında önemli bir artış görülmezken, GSH ve MDA düzeylerinde ise anlamlı bir azalma (P<0.05) olduğu belirlendi. Bu grup, vitamin E verilen grupla kıyaslandığında ise sadece MDA düzeylerinde anlamlı bir azalma (P<0.05) olduğu belirlendi. Sonuç olarak A. tuncelianum'un ratların bağırsak dokusunda, antioksidan enzim aktivitelerini ve lipit peroksidasyonunu olumlu bir şekilde değiştirdiği belirlendi. Böylece oksidatif hasara karşı, enzim aktivitelerini düzenleyerek ve lipit hasarını önleyerek katkı sağlayabileceği kanaatine varıldı.

Anahtar Kelimeler

Allium tuncelianum, Antioksidan enzim aktivitesi, DMBA, MDA

The Effect of Tunceli Mountain Garlic (Allium tuncelianum) and Vitamin E on Lipid Peroxidation and Antioxidant Enzyme Activities in the Intestinal Tissue of Dimethyl Benzanthracene-Administered Rats

Öz

The aim of this study was to determine the effect of Tunceli Mountain Garlic (Allium tuncelianum) and vitamin E on lipid peroxidation and antioxidant enzyme activities in rat intestinal tissue. Wistar albino type female rats were used in the study. Five groups, included 8 animals (n=8) in each, were formed. Group 1; Control group, Group 2; 7,12-dimethyl benzanthracene (DMBA) given group, Group 3; 7,12-DMBA in addition to 250 mg/kg/day Tunceli Mountain Garlic (TDS) given group, Group 4; 7,12-DMBA plus 500 mg/kg/day TDS given group and Group 5; 7,12-DMBA in addition to vitamin E 200 mg/kg (twice a week) was formed as a group. At the end of the 30-days trial rats were euthanized and intestinal tissue was removed and homogenized.Lipid peroxidation and antioxidant enzyme levels were measured in intestinal tissue homogenate by spectrophotometric method. At the end of the study, it was determined that catalase (CAT), total glutathione (GSH) and superoxide dismutase (SOD) enzyme activities in intestinal tissue of DMBA given group (Group 2) significantly decreased compared to the control group (P <0.05) while MDA level significantly increased (P <0.05). A significant increase (P <0.05) was observed in CAT and SOD levels in the group given low dose DDS and TDS (Group 3). When this group was compared with the vitamin E group, CAT and SOD levels were significantly increased (P <0.05) and GSH and MDA levels were significantly decreased (P <0.05). There was no significant increase in CAT and SOD enzyme levels in the group with high dose DDS and TDS (Group 4) compared with the patient group while there was a significant decrease in GSH and MDA levels (P <0.05). When this group is compared with the vitamin E group, only MDA levels were significantly decreased (P <0.05). In conclusion, it was determined that A. tuncelianum positively changed the antioxidant enzyme activities and lipid peroxidation in the intestinal tissue of rats. Thus, it was concluded that it can contribute to oxidative damage by regulating enzyme activities and preventing lipid damage.

Anahtar Kelimeler

Allium tuncelianum, Antioxidant enzyme activity, DMBA, MDA

Kaynakça

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