Yıl 2020, Cilt 9 , Sayı 1, Sayfalar 69 - 74 2020-06-25

Sinir Otu Etkin Maddesi Aucubinin İzole Rat Düz Kasları ve Primer Hücre Hatları Üzerine Etkisi
The Effect of Plantain Active Ingredient Aucubin on Isolated Rat Smooth Muscle Tissue and Primary Cell Lines

Emre ARSLANBAŞ [1] , Haki KARA [2] , Nergiz Hacer TURGUT [3] , Hüseyin GÜNGÖR [4] , Halef Okan Doğan DOĞAN [5] , Mustafa Ozan ATASOY [6] , Alper Serhat KUMRU [7]


Bu çalışmada, sinir otu (Plantago) etkin maddesi aucubinin in vitro rat idrar kesesi ve trakea dokusunda farmakodinamik ve primer akciğer ve böbrek hücre hatları üzerine hücre koruyucu etkilerinin incelenmesi amaçlandı. Çalışma, idrar kesesi dokusuna tekrarlı olarak asetilkolin, atropin, verapamil ve oksibutinin ile kalsiyumsuz ortamda Ca++ verilerek, trakea dokusuna ise tekrarlı asetilkolin, atropin, karbakol ve mekamilamin uygulamalarıyla yapıldı. Aynı zamanda, primer hücre hatları üzerinde hücre canlılığı, katalaz ve süperoksit dismutaz enzim aktivitelerinin ölçümü gerçekleştirildi. Çalışma sonucunda, aucubinin idrar kesesi ve trakea dokusunda gevşetici etkisi belirlendi. Aucubinin kolinerjik antagonist olduğu, ancak bu etkisini farklı subreseptörler (muskarinik-3 reseptörler) üzerinden gösterdiği düşünüldü. Ayrıca, artan konsantrasyonlarda (1-10 μM) primer akciğer ve böbrek hücre hatlarında hücre canlılığında kontrol grubu seviyesinde kalan istatistiksel olarak önemsiz bir artışa neden olurken, aynı hücre hatlarında 20 ve 50 μM konsantrasyonlarda hücre canlılığında önemli bir düşüşe neden olduğu belirlendi. Sonuç olarak, halk arasında tedavi amacıyla kullanılan sinir otunun ve etkin maddesi aucubinin solunum yolları daralması ve hastalıkları, akciğer ve böbrek hastalıkları, üriner inkontinans vb. durumlarda yararlı olabileceği kanaatine varıldı.
This study aims to assess the pharmacodynamics of aucubin, the active ingredient in plantain (Plantago), on in vitro rat bladder and trachea tissues, and explore its cell protective effects on primary lung and kidney cell lines. The study was carried out via repeated applications of acetylcholine, atropine, verapamil and oxybutynin, alongside Ca++ in a calcium-free environment, on urinary bladder tissue, and repeated applications of acetylcholine, atropine, carbachol and mecamylamine on trachea tissue. At the same time, cell viability and catalase and superoxide dismutase activity was measured on primary cell lines. The results indicate that aucubin has a relaxant effect on urinary bladder and trachea tissues. It is conceived that aucubin acts as a cholinergic antagonist through different subreceptors (muscarinic-3 receptors). The results also indicate that aucubin has a dose-dependent effect on primary lung and kidney cell lines, with increased cell viability, reduced cytotoxicity and antioxidant effects. In conclusion, it is suggested that plantain, which is used in folk medicine, and its active ingredient aucubin might have beneficial effects on bronchoconstriction and other respiratory conditions, as well as on pulmonary and renal diseases, urinary incontinence, etc.
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Birincil Dil en
Konular Veteriner Hekimlik
Bölüm Makaleler
Yazarlar

Orcid: 0000-0003-0030-7195
Yazar: Emre ARSLANBAŞ (Sorumlu Yazar)
Kurum: Atatürk University, Faculty of Veterinary Medicine, Pharmacology and Toxicology Department
Ülke: Turkey


Orcid: 0000-0002-0295-0582
Yazar: Haki KARA
Kurum: Sivas Cumhuriyet University, Faculty of Veterinary Medicine, Pharmacology and Toxicology Department
Ülke: Turkey


Orcid: 0000-0003-1700-2451
Yazar: Nergiz Hacer TURGUT
Kurum: Izmir Kâtip Çelebi University, Faculty of Pharmacy, Pharmacology Department
Ülke: Turkey


Orcid: 0000-0002-2506-3855
Yazar: Hüseyin GÜNGÖR
Kurum: Sivas Cumhuriyet University, Faculty of Veterinary Medicine, Pharmacology and Toxicology Department
Ülke: Turkey


Orcid: 0000-0001-8738-0760
Yazar: Halef Okan Doğan DOĞAN
Kurum: Sivas Cumhuriyet University, Faculty of Medicine, Medicinal Biochemistry Department
Ülke: Turkey


Orcid: 0000-0003-0096-6297
Yazar: Mustafa Ozan ATASOY
Kurum: Sivas Cumhuriyet University, Faculty of Veterinary Medicine, Department of Virology
Ülke: Turkey


Orcid: 0000-0001-8462-4264
Yazar: Alper Serhat KUMRU
Kurum: Sivas Cumhuriyet University, Faculty of Veterinary Medicine, Pharmacology and Toxicology Department
Ülke: Turkey


Destekleyen Kurum Scientific Research Project Fund of Sivas Cumhuriyet University
Proje Numarası V-055 (CÜBAP, Sivas, Turkey)
Teşekkür This work is supported by the Scientific Research Project Fund of Sivas Cumhuriyet University under the project number V-055 (CÜBAP, Sivas, Turkey).
Tarihler

Başvuru Tarihi : 15 Mayıs 2020
Kabul Tarihi : 18 Haziran 2020
Yayımlanma Tarihi : 25 Haziran 2020

Bibtex @araştırma makalesi { huvfd733942, journal = {Harran Üniversitesi Veteriner Fakültesi Dergisi}, issn = {}, eissn = {2146-7188}, address = {Harran Üniversitesi Veteriner Fakültesi Dergisi Editörlüğü, Eyyübiye Kampüsü, 63200, Şanlıurfa}, publisher = {Harran Üniversitesi}, year = {2020}, volume = {9}, pages = {69 - 74}, doi = {10.31196/huvfd.733942}, title = {The Effect of Plantain Active Ingredient Aucubin on Isolated Rat Smooth Muscle Tissue and Primary Cell Lines}, key = {cite}, author = {Arslanbaş, Emre and Kara, Haki and Turgut, Nergiz Hacer and Güngör, Hüseyin and Doğan, Halef Okan Doğan and Atasoy, Mustafa Ozan and Kumru, Alper Serhat} }
APA Arslanbaş, E , Kara, H , Turgut, N , Güngör, H , Doğan, H , Atasoy, M , Kumru, A . (2020). The Effect of Plantain Active Ingredient Aucubin on Isolated Rat Smooth Muscle Tissue and Primary Cell Lines . Harran Üniversitesi Veteriner Fakültesi Dergisi , 9 (1) , 69-74 . DOI: 10.31196/huvfd.733942
MLA Arslanbaş, E , Kara, H , Turgut, N , Güngör, H , Doğan, H , Atasoy, M , Kumru, A . "The Effect of Plantain Active Ingredient Aucubin on Isolated Rat Smooth Muscle Tissue and Primary Cell Lines" . Harran Üniversitesi Veteriner Fakültesi Dergisi 9 (2020 ): 69-74 <https://dergipark.org.tr/tr/pub/huvfd/issue/55210/733942>
Chicago Arslanbaş, E , Kara, H , Turgut, N , Güngör, H , Doğan, H , Atasoy, M , Kumru, A . "The Effect of Plantain Active Ingredient Aucubin on Isolated Rat Smooth Muscle Tissue and Primary Cell Lines". Harran Üniversitesi Veteriner Fakültesi Dergisi 9 (2020 ): 69-74
RIS TY - JOUR T1 - The Effect of Plantain Active Ingredient Aucubin on Isolated Rat Smooth Muscle Tissue and Primary Cell Lines AU - Emre Arslanbaş , Haki Kara , Nergiz Hacer Turgut , Hüseyin Güngör , Halef Okan Doğan Doğan , Mustafa Ozan Atasoy , Alper Serhat Kumru Y1 - 2020 PY - 2020 N1 - doi: 10.31196/huvfd.733942 DO - 10.31196/huvfd.733942 T2 - Harran Üniversitesi Veteriner Fakültesi Dergisi JF - Journal JO - JOR SP - 69 EP - 74 VL - 9 IS - 1 SN - -2146-7188 M3 - doi: 10.31196/huvfd.733942 UR - https://doi.org/10.31196/huvfd.733942 Y2 - 2020 ER -
EndNote %0 Harran Üniversitesi Veteriner Fakültesi Dergisi The Effect of Plantain Active Ingredient Aucubin on Isolated Rat Smooth Muscle Tissue and Primary Cell Lines %A Emre Arslanbaş , Haki Kara , Nergiz Hacer Turgut , Hüseyin Güngör , Halef Okan Doğan Doğan , Mustafa Ozan Atasoy , Alper Serhat Kumru %T The Effect of Plantain Active Ingredient Aucubin on Isolated Rat Smooth Muscle Tissue and Primary Cell Lines %D 2020 %J Harran Üniversitesi Veteriner Fakültesi Dergisi %P -2146-7188 %V 9 %N 1 %R doi: 10.31196/huvfd.733942 %U 10.31196/huvfd.733942
ISNAD Arslanbaş, Emre , Kara, Haki , Turgut, Nergiz Hacer , Güngör, Hüseyin , Doğan, Halef Okan Doğan , Atasoy, Mustafa Ozan , Kumru, Alper Serhat . "The Effect of Plantain Active Ingredient Aucubin on Isolated Rat Smooth Muscle Tissue and Primary Cell Lines". Harran Üniversitesi Veteriner Fakültesi Dergisi 9 / 1 (Haziran 2020): 69-74 . https://doi.org/10.31196/huvfd.733942
AMA Arslanbaş E , Kara H , Turgut N , Güngör H , Doğan H , Atasoy M , Kumru A . The Effect of Plantain Active Ingredient Aucubin on Isolated Rat Smooth Muscle Tissue and Primary Cell Lines. Harran Univ Vet Fak Derg. 2020; 9(1): 69-74.
Vancouver Arslanbaş E , Kara H , Turgut N , Güngör H , Doğan H , Atasoy M , Kumru A . The Effect of Plantain Active Ingredient Aucubin on Isolated Rat Smooth Muscle Tissue and Primary Cell Lines. Harran Üniversitesi Veteriner Fakültesi Dergisi. 2020; 9(1): 69-74.
IEEE E. Arslanbaş , H. Kara , N. Turgut , H. Güngör , H. Doğan , M. Atasoy ve A. Kumru , "The Effect of Plantain Active Ingredient Aucubin on Isolated Rat Smooth Muscle Tissue and Primary Cell Lines", Harran Üniversitesi Veteriner Fakültesi Dergisi, c. 9, sayı. 1, ss. 69-74, Haz. 2020, doi:10.31196/huvfd.733942