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Effect of Propolis Applied to Goat Kids at Weaning Period on Heat Shock Protein Genes

Yıl 2024, Cilt: 13 Sayı: 2, 84 - 92, 18.12.2024
https://doi.org/10.31196/huvfd.1451671

Öz

In recent years, studies on the use of natural and organic additives have gained importance in goat breeding in order to prevent offspring losses and to encourage their growth and development by limiting the use of antibiotics. Especially the weaning period is a stressful period for kids and negative effects such as weight loss, increased susceptibility to viral and bacterial infections may be observed as a result of decreased nutrient intake and utilisation during this period. Considering these disadvantages that occur during the weaning period, it was thought that propolis would increase the potential to protect the health and welfare of kids during the weaning period due to its immunomodulatory, antioxidant, and anti-inflammatory effects. Therefore, we aimed to examine the expression levels of HSP27, HSP60, and HSP70 molecular chaperones that modulate the cellular stress response, which partially express the effects of propolis on weaning stress. Saanen kids were divided into propolis treated (n=10) and control (no propolis treatment; n=10) groups. The propolis-treated group received 0.4 cc propolis once a day for two weeks after weaning. Expression levels were calculated by 2-ΔΔCt using the Pfaffl method and statistical significance levels were determined by Student t test. Blood samples were taken on the day of weaning and the following day to determine the effect of weaning stress on HSP27, HSP60, and HSP70 expression levels. The effect of propolis on weaning stress was examined in samples taken after two weeks of propolis treatment. The expression levels of HSP27 and HSP60 increased by approximately 2-fold during weaning stress, while HSP70 increased by 3.35-fold. When 0.4 cc propolis was applied to kids under weaning stress, a statistically significant downregulation of HSP27 level 1.08-fold, HSP60 level 1.56-fold, and HSP70 level 2.12-fold was obtained at the end of 2 weeks compared to the control group. Our study showed that propolis treatment decreased stress protein levels during weaning stress.

Kaynakça

  • Abd-Allah M, Daghash MWH, 2019: Influence of using flavomycin and propolis as feed additives on buffalo milk production, and growth per-formance and blood metabolites of suckling calves. Egypt J Nutr and Feeds, 22 (1), 13-22.
  • Aguiar SC, Cottica SM, Boeing JS, Samensari RB, Santos GT, Visentainer JV, Zeoula LM, 2014: Effect of feeding phenolic compounds from propolis extracts to dairy cows on milk production, milk fatty acid composition, and the antioxidant capacity of milk. Anim Feed Sci Technol, 193, 148-154.
  • Arvans DL, Vavricka SR, Ren H, Musch MW, Kang L, Rocha FG, Lucioni A, Turner JR, Alverdy J, Chang EB, 2005: Luminal bacterial flora determines physiological expression of intestinal epithelial cytoprotective heat shock proteins 25 and 72. Am J Physiol Gastrointest Liver Physiol, 288 (4), G696-G704.
  • Badawy HS, 2021: Effect of propolis as a feed additive on nutritional and productive performance of pregnant ewes and their lambs under Halaib-Shalateen pastures condition. J Anim Poul Pro, 12 (1), 19-26.
  • Baldwin RL, McLeod KR, Klotz JL, Heitmann RN, 2004: Rumen development, intestinal growth and hepatic metabolism in the pre- and post-weaning ruminant. J Dairy Sci, 87, E55- E65.
  • Belanche A, Palma-Hidalgo JM, Nejjam I, Jiménez E, Martín-García AI, Yáñez-Ruiz, DR, 2020: Inoculation with rumen fluid in early life as a strategy to optimize the weaning process in intensive dairy goat systems. J Dairy Sci, 103 (6), 5047-5060.
  • Cécere BG, da Silva AS, Molosse VL, Alba DF, Leal KW, da Rosa G, Pereira WAB, da Silva AD, Schetinger MRC, Kempka AB, Nunes A, Maraschin M, Araújo DN, Deolindo GL, Vedovatto M, 2021: Addition of propolis to milk improves lactating lamb’s growth: Effect on antimicrobial, antioxidant and immune responses in animals. Small Ruminant Res 194, 106265.
  • Chauhan I, Misra S, Kumar A, Gowane GR, 2019: Survival analysis of mortality in pre-weaning kids of Sirohi goat. Animal, 13 (12), 2896-2902.
  • Chen K, Liu Y, Cheng Y, Yan Q, Zhou C, He Z, Zeng J, He J, Tan Z, 2020: Supplementation of lactobacillus plantarum or macleaya cordata extract alleviates oxidative damage ınduced by weaning in the lower gut of young goats. Animals, 10 (4), 548.
  • Choi YK, Jo PG, Choi CY, 2008: Cadmium affects the expression of heat shock protein 90 and metallothionein mRNA in the Pacific oyster, Crassostrea gigas. Comp Biochem Physiol -C Toxicol Pharmacol, 147 (3), 286-292. Cox LN, Cooper JJ, 2001: Observations on the pre-and post-weaning behaviour of piglets reared in commercial indoor and outdoor environments. Anim Sci, 72 (1), 75-86.
  • Dangi SS, Gupta M, Nagar V, Yadav VP, Dangi SK, Shankar O, Chouhan VS, Kumar P, Singh G, Sarkar M, 2014: Impact of short-term heat stress on physiological responses and expression profile of HSPs in Barbari goats. Int J Biometeorol, 58, 2085-2093.
  • Datt M, Bhateshwar V, Rai DC, 2023: Importance of body weight, age and body condition in weaning of goat kids: a review. J Livest Sci, 14, 71-77.
  • Degroote J, Vergauwen H, Wang W, Van Ginneken C, De Smet S, Michiels J, 2020: Changes of the glutathione redox system during the weaning transition in piglets, in relation to small intestinal morphology and barrier function. J Anim Sci Biotechnol, 11, 1-17.
  • Durosaro SO, Iyasere OS, Ilori BM, Oyeniran VJ, Ozoje MO, 2023: Molecular regulation, breed differences and genes involved in stress control in farm animals. Domest Anim Endocrinol, 82, 106769.
  • Ehrenfried JA, Herron BE, Townsend Jr CM, Evers BM, 1995: Heat shock proteins are differentially expressed in human gastrointestinal cancers. Surg Oncol, 4 (4), 197-203.
  • Fiesel A, Gessner DK, Most E, Eder K, 2014: Effects of dietary polyphenol-rich plant products from grape or hop on pro-inflammatory gene expression in the intestine, nutrient digestibility and faecal microbiota of weaned pigs. BMC Vet Res, 10, 196.
  • Grundtman C, Kreutmayer SB, Almanzar G, Wick MC, Wick G, 2011: Heat shock protein 60 and immune inflammatory responses in atherosclerosis. Arterioscler Thromb Vasc Biol, 31 (5), 960-968.
  • Hussain T, Wang J, Murtaza G, Metwally E, Yang H, Kalhoro MS, Kalhoro DH, Rahu BA, Tan B, Sahito RGA, Chughtai MI, Yin Y, 2021: The role of polyphenols in regulation of heat shock proteins and gut microbiota in weaning stress. Oxid Med Cell Longev, 2021 (1).
  • Ismael AB, El-Sayed EM, Sameh Mohammed EN, 2019: Potential clinical role of propolis in treatment of clinical ovine and caprine listeriosis. Vet Med J, 57, 301-309.
  • Jeppesen LL, Heller KE, Dalsgaard T, 2000: Effects of early weaning and housing conditions on the development of stereotypies in farmed mink. App Anim Behav Sci, 68 (1), 85-92.
  • Kazemi S, Hajimohammadi A, Mirzaei A, Nazifi S, 2023: Effects of probiotic and yeast extract supplementation on oxidative stress, inflammatory response, and growth in weaning Saanen kids. Trop Anim Health Prod, 55, 282.
  • Khan MA, Bach A, Weary DM, von Keyserlingk MAG, 2016: Transitioning from milk to solid feed in dairy heifers. J Dairy Sci, 99, 885-902.
  • Korte SM, Olivier B, Koolhaas JM, 2007: A new animal welfare concept based on allostasis. Physiol Behav, 92 (3), 422-428. Kresnoadi U, Rahayu RP, Ariani MD, Soesanto S, 2020: The potential of natural propolis extract combined with bovine bone graft in increasing heat shock protein 70 and osteocalcin on socket preservation. Eur J Dent, 14 (1), 31-37.
  • LeBlanc S, Höglund E, Gilmour KM, Currie S, 2012: Hormonal modulation of the heat shock response: insights from fish with divergent cortisol stress responses. Am J Physiol Regul Integr Comp Physiol, 302 (1), 184-192.
  • Li Y, Li L, Yang J, Lv L, Jin T, 2018: Effect of piglet weaning age on mRNA and protein expression of heat shock proteins. Int J Agric Biol, 20 (10), 2335-2341.
  • Liberek K, Lewandowska A, Zietkiewicz S, 2008: Chaperones in control of protein disaggregation. The EMBO J, 27 (2), 328-335.
  • Livak KJ, Schmittgen TD, 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCT method. Methods, 25 (4), 402-408.
  • Liyanagamage DSNK, Martinus RD, 2020: Role of mitochondrial stress protein HSP60 in diabetes-induced neuroinflammation. Mediators Inflamm, Article ID 8073516.
  • Ludwig D, Stahl M, Ibrahim M, Wenzel B, Drabicki D, Wecke A, Fellermann K, Stange E, 1999: Enhanced ıntestinal expression of heat shock protein 70 in patients with ınflammatory bowel diseases. Dig Dis Sci, 44 (7), 1440-1447.
  • Lynch E, McGee M, Earley B, 2019: Weaning management of beef calves with implications for animal health and welfare. J Applied Anim Res, 47 (1), 167-175.
  • Manav S, Yilmaz M, 2023: Effect of whey and propolis on growth characteristics, on blood values and diarrhea of the goat kids. Vet Arh, 93 (2), 223-238.
  • Mehlen P, Mehlen A, Guillet D, Preville X, Arrigo AP, 1995: Tumor necrosis factor‐α induces changes in the phosphorylation, cellular localization, and oligomerization of human hsp27, a stress protein that confers cellular resistance to this cytokine. J Cell Biochem, 58 (2), 248-259.
  • Mogk A, Mayer MP, Deuerling E, 2002: Mechanisms of protein folding: molecular chaperones and their application in biotechnology. Chembiochem, 3 (9), 807-814.
  • Moreno-Indias I, Sánchez-Alcoholado L, Pérez-Martínez P, Andrés-Lacueva C, Cardona F, Tinahones F, Queipo-Ortuño MI, 2016: Red wine polyphenols modulate fecal microbiota and reduce markers of the metabolic syndrome in obese patients. Food Funct, 7 (4), 1775-1787.
  • Morsy AS, Abdalla AL, Soltan YA, Sallam SM, El-Azrak KEDM, Louvandini H, Alencar S, 2013: Effect of Brazilian red propolis administration on hematological, biochemical variables and parasitic response of Santa Inês ewes during and after flushing period. Trop Anim Health Prod, 45 (7), 1609-1618.
  • Mosele JI, Macià A, Motilva MJ, 2015: Metabolic and microbial modulation of the large intestine ecosystem by non-absorbed diet phenolic compounds: a review. Molecules, 20 (9), 17429-17468.
  • Nugroho N, Alamsyah R, Wardani AS, Maulana H, Marbelia L, Tanto D, Baliarti E, 2023: The effect of palm oil mill effluent supplementation on post-weaning goat performance. In IOP Conference Series: JEES. (Vol. 1174, No. 1, p. 012018). IOP Publishing.
  • Oliveira JSD, Queiroz ACD, Lana RDP, Mantovani HC, Generoso RAR, 2006: Efeito da monensina e da própolis sobre a atividade de fermentação de aminoácidos in vitro pelos microrganismos ruminais. Rev Bras Zootecn, 35 (1), 275-281.
  • Otaka M, Odashima M, Watanabe S, 2006: Role of heat shock proteins (molecular chaperones) in intestinal mucosal protection. Biochem Biophys Res Commun, 348 (1), 1-5.
  • Öztürk E, Kahveci N, Özlük K, Yılmazlar T, 2009: Isı şok proteinleri. Turk J Sur, 25 (4), 131-136.
  • Pistol GC, Marin DE, Bulgaru VC, Anghel AC, Sărăcilă M, Vlassa M, Filip M, Taranu I, 2023: Grape seed meal by-product is able to counteract oxidative stress induced by lipopolysaccharide and dextran sulphate in IPEC cells and piglets after weaning. PLoS ONE, 18 (4), e0283607.
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Sütten Kesim Dönemindeki Oğlaklara Uygulanan Propolisin Isı Şok Protein Genlerine Etkisi

Yıl 2024, Cilt: 13 Sayı: 2, 84 - 92, 18.12.2024
https://doi.org/10.31196/huvfd.1451671

Öz

Son yıllarda keçi yetiştiriciliğinde, yavru kayıplarını önlemek ve antibiyotik kullanımını sınırlandırarak büyüme ve gelişmelerini teşvik etmek amacıyla doğal ve organik katkı maddeleri kullanımına yönelik çalışmalar önem kazanmıştır. Özellikle sütten kesme dönemi oğlaklar için stresli bir dönem olup, bu süreçte besin alımının ve yararlanımının azalması sonucu kilo kaybı, viral ve bakteriyel enfeksiyonlara karşı artan duyarlılık gibi olumsuz etkiler gözlemlenebilir. Sütten kesim döneminde ortaya çıkan bu dezavantajlar düşünüldüğünde propolisin immünmodülatör, antioksidan ve antiinflamatuvar etkilerinden dolayı sütten kesim döneminde oğlakların sağlığını koruma ve refahını arttıracağı düşünülmüştür. Bu nedenle çalışmada propolisin sütten kesme stresi üzerindeki etkilerini kısmen ifade eden, hücresel stres tepkisini modüle eden HSP27, HSP60 ve HSP70 moleküler şaperonların ekspresyon seviyelerini incelemeyi amaçladık. Saanen oğlakları propolis uygulanan (n=10) ve kontrol (propolis uygulanmayan; n=10) gruplarına ayrıldı. Propolis uygulanan gruba sütten kesildikten sonra 2 hafta boyunca günde bir kez 0,4 cc propolis verildi. Ekspresyon seviyeleri Pfaffl yöntemi kullanılarak 2-ΔΔCt ile hesaplandı ve student t testi ile istatistiksel önem düzeyleri belirlendi. Sütten kesme stresinin HSP27, HSP60 ve HSP70 ekspresyon seviyeleri üzerindeki etkisini belirlemek için sütten kesim günü ve ertesi gün kan örnekleri alındı. Propolisin sütten kesme stresi üzerindeki etkisi, 2 haftalık propolis uygulamasından sonra alınan örneklerde incelendi. Oğlaklarda sütten kesme stresi sırasında HSP27 ve HSP60’in ekspresyon seviyeleri yaklaşık 2 kat artarken, HSP70 3,35 kat artmıştır. Sütten kesme stresi altındaki oğlaklara 0,4 cc propolis uygulandığında 2 haftanın sonunda kontrol grubuna göre HSP27 seviyesinde 1,08 kat, HSP60 seviyesinde 1,56 kat, HSP70 seviyesinde ise 2,12 kat istatistiksel olarak anlamlı downregülasyon elde edildi. Çalışmamız propolis uygulamasının sütten kesme stresi sırasında stres protein düzeylerini azalttığını gösterdi.

Kaynakça

  • Abd-Allah M, Daghash MWH, 2019: Influence of using flavomycin and propolis as feed additives on buffalo milk production, and growth per-formance and blood metabolites of suckling calves. Egypt J Nutr and Feeds, 22 (1), 13-22.
  • Aguiar SC, Cottica SM, Boeing JS, Samensari RB, Santos GT, Visentainer JV, Zeoula LM, 2014: Effect of feeding phenolic compounds from propolis extracts to dairy cows on milk production, milk fatty acid composition, and the antioxidant capacity of milk. Anim Feed Sci Technol, 193, 148-154.
  • Arvans DL, Vavricka SR, Ren H, Musch MW, Kang L, Rocha FG, Lucioni A, Turner JR, Alverdy J, Chang EB, 2005: Luminal bacterial flora determines physiological expression of intestinal epithelial cytoprotective heat shock proteins 25 and 72. Am J Physiol Gastrointest Liver Physiol, 288 (4), G696-G704.
  • Badawy HS, 2021: Effect of propolis as a feed additive on nutritional and productive performance of pregnant ewes and their lambs under Halaib-Shalateen pastures condition. J Anim Poul Pro, 12 (1), 19-26.
  • Baldwin RL, McLeod KR, Klotz JL, Heitmann RN, 2004: Rumen development, intestinal growth and hepatic metabolism in the pre- and post-weaning ruminant. J Dairy Sci, 87, E55- E65.
  • Belanche A, Palma-Hidalgo JM, Nejjam I, Jiménez E, Martín-García AI, Yáñez-Ruiz, DR, 2020: Inoculation with rumen fluid in early life as a strategy to optimize the weaning process in intensive dairy goat systems. J Dairy Sci, 103 (6), 5047-5060.
  • Cécere BG, da Silva AS, Molosse VL, Alba DF, Leal KW, da Rosa G, Pereira WAB, da Silva AD, Schetinger MRC, Kempka AB, Nunes A, Maraschin M, Araújo DN, Deolindo GL, Vedovatto M, 2021: Addition of propolis to milk improves lactating lamb’s growth: Effect on antimicrobial, antioxidant and immune responses in animals. Small Ruminant Res 194, 106265.
  • Chauhan I, Misra S, Kumar A, Gowane GR, 2019: Survival analysis of mortality in pre-weaning kids of Sirohi goat. Animal, 13 (12), 2896-2902.
  • Chen K, Liu Y, Cheng Y, Yan Q, Zhou C, He Z, Zeng J, He J, Tan Z, 2020: Supplementation of lactobacillus plantarum or macleaya cordata extract alleviates oxidative damage ınduced by weaning in the lower gut of young goats. Animals, 10 (4), 548.
  • Choi YK, Jo PG, Choi CY, 2008: Cadmium affects the expression of heat shock protein 90 and metallothionein mRNA in the Pacific oyster, Crassostrea gigas. Comp Biochem Physiol -C Toxicol Pharmacol, 147 (3), 286-292. Cox LN, Cooper JJ, 2001: Observations on the pre-and post-weaning behaviour of piglets reared in commercial indoor and outdoor environments. Anim Sci, 72 (1), 75-86.
  • Dangi SS, Gupta M, Nagar V, Yadav VP, Dangi SK, Shankar O, Chouhan VS, Kumar P, Singh G, Sarkar M, 2014: Impact of short-term heat stress on physiological responses and expression profile of HSPs in Barbari goats. Int J Biometeorol, 58, 2085-2093.
  • Datt M, Bhateshwar V, Rai DC, 2023: Importance of body weight, age and body condition in weaning of goat kids: a review. J Livest Sci, 14, 71-77.
  • Degroote J, Vergauwen H, Wang W, Van Ginneken C, De Smet S, Michiels J, 2020: Changes of the glutathione redox system during the weaning transition in piglets, in relation to small intestinal morphology and barrier function. J Anim Sci Biotechnol, 11, 1-17.
  • Durosaro SO, Iyasere OS, Ilori BM, Oyeniran VJ, Ozoje MO, 2023: Molecular regulation, breed differences and genes involved in stress control in farm animals. Domest Anim Endocrinol, 82, 106769.
  • Ehrenfried JA, Herron BE, Townsend Jr CM, Evers BM, 1995: Heat shock proteins are differentially expressed in human gastrointestinal cancers. Surg Oncol, 4 (4), 197-203.
  • Fiesel A, Gessner DK, Most E, Eder K, 2014: Effects of dietary polyphenol-rich plant products from grape or hop on pro-inflammatory gene expression in the intestine, nutrient digestibility and faecal microbiota of weaned pigs. BMC Vet Res, 10, 196.
  • Grundtman C, Kreutmayer SB, Almanzar G, Wick MC, Wick G, 2011: Heat shock protein 60 and immune inflammatory responses in atherosclerosis. Arterioscler Thromb Vasc Biol, 31 (5), 960-968.
  • Hussain T, Wang J, Murtaza G, Metwally E, Yang H, Kalhoro MS, Kalhoro DH, Rahu BA, Tan B, Sahito RGA, Chughtai MI, Yin Y, 2021: The role of polyphenols in regulation of heat shock proteins and gut microbiota in weaning stress. Oxid Med Cell Longev, 2021 (1).
  • Ismael AB, El-Sayed EM, Sameh Mohammed EN, 2019: Potential clinical role of propolis in treatment of clinical ovine and caprine listeriosis. Vet Med J, 57, 301-309.
  • Jeppesen LL, Heller KE, Dalsgaard T, 2000: Effects of early weaning and housing conditions on the development of stereotypies in farmed mink. App Anim Behav Sci, 68 (1), 85-92.
  • Kazemi S, Hajimohammadi A, Mirzaei A, Nazifi S, 2023: Effects of probiotic and yeast extract supplementation on oxidative stress, inflammatory response, and growth in weaning Saanen kids. Trop Anim Health Prod, 55, 282.
  • Khan MA, Bach A, Weary DM, von Keyserlingk MAG, 2016: Transitioning from milk to solid feed in dairy heifers. J Dairy Sci, 99, 885-902.
  • Korte SM, Olivier B, Koolhaas JM, 2007: A new animal welfare concept based on allostasis. Physiol Behav, 92 (3), 422-428. Kresnoadi U, Rahayu RP, Ariani MD, Soesanto S, 2020: The potential of natural propolis extract combined with bovine bone graft in increasing heat shock protein 70 and osteocalcin on socket preservation. Eur J Dent, 14 (1), 31-37.
  • LeBlanc S, Höglund E, Gilmour KM, Currie S, 2012: Hormonal modulation of the heat shock response: insights from fish with divergent cortisol stress responses. Am J Physiol Regul Integr Comp Physiol, 302 (1), 184-192.
  • Li Y, Li L, Yang J, Lv L, Jin T, 2018: Effect of piglet weaning age on mRNA and protein expression of heat shock proteins. Int J Agric Biol, 20 (10), 2335-2341.
  • Liberek K, Lewandowska A, Zietkiewicz S, 2008: Chaperones in control of protein disaggregation. The EMBO J, 27 (2), 328-335.
  • Livak KJ, Schmittgen TD, 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCT method. Methods, 25 (4), 402-408.
  • Liyanagamage DSNK, Martinus RD, 2020: Role of mitochondrial stress protein HSP60 in diabetes-induced neuroinflammation. Mediators Inflamm, Article ID 8073516.
  • Ludwig D, Stahl M, Ibrahim M, Wenzel B, Drabicki D, Wecke A, Fellermann K, Stange E, 1999: Enhanced ıntestinal expression of heat shock protein 70 in patients with ınflammatory bowel diseases. Dig Dis Sci, 44 (7), 1440-1447.
  • Lynch E, McGee M, Earley B, 2019: Weaning management of beef calves with implications for animal health and welfare. J Applied Anim Res, 47 (1), 167-175.
  • Manav S, Yilmaz M, 2023: Effect of whey and propolis on growth characteristics, on blood values and diarrhea of the goat kids. Vet Arh, 93 (2), 223-238.
  • Mehlen P, Mehlen A, Guillet D, Preville X, Arrigo AP, 1995: Tumor necrosis factor‐α induces changes in the phosphorylation, cellular localization, and oligomerization of human hsp27, a stress protein that confers cellular resistance to this cytokine. J Cell Biochem, 58 (2), 248-259.
  • Mogk A, Mayer MP, Deuerling E, 2002: Mechanisms of protein folding: molecular chaperones and their application in biotechnology. Chembiochem, 3 (9), 807-814.
  • Moreno-Indias I, Sánchez-Alcoholado L, Pérez-Martínez P, Andrés-Lacueva C, Cardona F, Tinahones F, Queipo-Ortuño MI, 2016: Red wine polyphenols modulate fecal microbiota and reduce markers of the metabolic syndrome in obese patients. Food Funct, 7 (4), 1775-1787.
  • Morsy AS, Abdalla AL, Soltan YA, Sallam SM, El-Azrak KEDM, Louvandini H, Alencar S, 2013: Effect of Brazilian red propolis administration on hematological, biochemical variables and parasitic response of Santa Inês ewes during and after flushing period. Trop Anim Health Prod, 45 (7), 1609-1618.
  • Mosele JI, Macià A, Motilva MJ, 2015: Metabolic and microbial modulation of the large intestine ecosystem by non-absorbed diet phenolic compounds: a review. Molecules, 20 (9), 17429-17468.
  • Nugroho N, Alamsyah R, Wardani AS, Maulana H, Marbelia L, Tanto D, Baliarti E, 2023: The effect of palm oil mill effluent supplementation on post-weaning goat performance. In IOP Conference Series: JEES. (Vol. 1174, No. 1, p. 012018). IOP Publishing.
  • Oliveira JSD, Queiroz ACD, Lana RDP, Mantovani HC, Generoso RAR, 2006: Efeito da monensina e da própolis sobre a atividade de fermentação de aminoácidos in vitro pelos microrganismos ruminais. Rev Bras Zootecn, 35 (1), 275-281.
  • Otaka M, Odashima M, Watanabe S, 2006: Role of heat shock proteins (molecular chaperones) in intestinal mucosal protection. Biochem Biophys Res Commun, 348 (1), 1-5.
  • Öztürk E, Kahveci N, Özlük K, Yılmazlar T, 2009: Isı şok proteinleri. Turk J Sur, 25 (4), 131-136.
  • Pistol GC, Marin DE, Bulgaru VC, Anghel AC, Sărăcilă M, Vlassa M, Filip M, Taranu I, 2023: Grape seed meal by-product is able to counteract oxidative stress induced by lipopolysaccharide and dextran sulphate in IPEC cells and piglets after weaning. PLoS ONE, 18 (4), e0283607.
  • Rogalla T, Ehrnsperger M, Preville X, Kotlyarov A, Lutsch G, Ducasse C, Paul C, Wieske M, Arrigo AP, Buchner J, Gaestel M, 1999: Regulation of Hsp27 oligomerization, chaperone function, and protective activity against oxidative stress/tumor necrosis factor α by phosphorylation. J Biol Chem, 274 (27), 18947-18956.
  • Sadek WMA, El-Houssiny AS, Al-Mwafy A, Farag TK, Al-Gethami A, Grawish SIM, Hegazi AG, 2020: Egyptian propolis 16: The effect of consumption of propolis and alginate-propolis nanoparticles in combination with colostrum on the performance of newborn goats. Adv Anim Vet Sci, 8 (12), 1256-1265.
  • Sala AJ, Bott LC, Morimoto RI, 2017: Shaping proteostasis at the cellular, tissue, and organismal level. J Cell Biol, 216 (5), 1231-1241.
  • Sarker MSK, Yang CJ, 2010: Propolis and illite as feed additives on performance and blood profiles of post-weaning Hanwoo calves. J Anim Vet Adv, 9 (21), 2704-2709.
  • Satitsri S, Akrimajirachoote N, Nunta K, Ruennarong N, Amnucksoradej O, Muanprasat C, 2023: Piperine as potential therapy of post-weaning porcine diarrheas: an in vitro study using a porcine duodenal enteroid model. BMC Vet Res, 19 (1), 1-13.
  • Selma MV, Espín JC, Tomás-Barberán FA, 2009: Interaction between phenolics and gut microbiota: role in human health. J Agric Food Chem, 57 (15), 6485-6501.
  • Serrano-Jara D, Rivera-Gomis J, Tornel JA, Bernabé A, Martínez-Conesa C, Navarro JA, Cánovas R, Otal J, Cubero MJ, 2023: Effects of dietary supplementation with purple garlic powder and oregano essential oil on intestinal health in post-weaning piglets from commercial farms. Vet Res Commun, 47 (2), 901-909.
  • Shedeed HA, Farrag B, Elwakeel EA, Abd El-Hamid IS, El-Rayes MAH, 2019: Propolis supplementation improved productivity, oxidative status, and immune response of Barki ewes and lambs. Vet World, 12 (6), 834-843.
  • Stradiotti Júnior D, Queiroz ACD, Lana RDP, Pacheco CG, Eifert EDC, Nunes PMM, 2004: Effect of the propolis on amino acids deamination and ruminal fermentation. R Bras Zootec, 33 (4), 1086-1092.
  • Teh TH, Potchoiba MJ, Escobar EN, Lu CD, 1984: Weaning methods of goat kids. J Dairy Sci, 67, 137.
  • Tsugami Y, Nii T, Kobayashi K, Isobe N, 2023: Potential effects of gingerol topical application on components of the innate immunity in lactating goat mammary glands. Innate Immun, 29 (7), 135-149.
  • Vickery HM, Neal RA, Stergiadis S, Meagher RK 2023: Gradually weaning goat kids may improve weight gains while reducing weaning stress and increasing creep feed intakes. Front Vet Sci, 10, 1200849.
  • Yılmaz M, Kaykı M, Ekren Aşıcı GS, Kıral F, 2018: The mRNA gene expression profiles for HSP60 and HSP70 in various aged Saanen goats in different seasons. Kafkas Univ Vet Fak Derg, 24 (1), 75-81.
  • Zawadzki F, Prado IN, Marques JA, Zeoula LM, Rotta PP, Sestari BB, Valero MV, Rivaroli DC, 2011: Sodium monensin or propolis extract in the diets of feedlot-finished bulls: effects on animal performance and carcass characteristics. J Anim Feed Sci, 20 (1), 16-25.
Toplam 55 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Veteriner Biyokimya
Bölüm Araştıma
Yazarlar

Gamze Sevri Ekren Aşıcı 0000-0002-9625-7956

Alkan Çağlı 0000-0001-8737-1290

Hasan Çoğan 0000-0002-5979-9318

Funda Kargın Kıral 0000-0001-9348-8401

Murat Yılmaz 0000-0001-5338-0244

Yayımlanma Tarihi 18 Aralık 2024
Gönderilme Tarihi 15 Mart 2024
Kabul Tarihi 9 Eylül 2024
Yayımlandığı Sayı Yıl 2024 Cilt: 13 Sayı: 2

Kaynak Göster

APA Ekren Aşıcı, G. S., Çağlı, A., Çoğan, H., Kargın Kıral, F., vd. (2024). Effect of Propolis Applied to Goat Kids at Weaning Period on Heat Shock Protein Genes. Harran Üniversitesi Veteriner Fakültesi Dergisi, 13(2), 84-92. https://doi.org/10.31196/huvfd.1451671
AMA Ekren Aşıcı GS, Çağlı A, Çoğan H, Kargın Kıral F, Yılmaz M. Effect of Propolis Applied to Goat Kids at Weaning Period on Heat Shock Protein Genes. Harran Univ Vet Fak Derg. Aralık 2024;13(2):84-92. doi:10.31196/huvfd.1451671
Chicago Ekren Aşıcı, Gamze Sevri, Alkan Çağlı, Hasan Çoğan, Funda Kargın Kıral, ve Murat Yılmaz. “Effect of Propolis Applied to Goat Kids at Weaning Period on Heat Shock Protein Genes”. Harran Üniversitesi Veteriner Fakültesi Dergisi 13, sy. 2 (Aralık 2024): 84-92. https://doi.org/10.31196/huvfd.1451671.
EndNote Ekren Aşıcı GS, Çağlı A, Çoğan H, Kargın Kıral F, Yılmaz M (01 Aralık 2024) Effect of Propolis Applied to Goat Kids at Weaning Period on Heat Shock Protein Genes. Harran Üniversitesi Veteriner Fakültesi Dergisi 13 2 84–92.
IEEE G. S. Ekren Aşıcı, A. Çağlı, H. Çoğan, F. Kargın Kıral, ve M. Yılmaz, “Effect of Propolis Applied to Goat Kids at Weaning Period on Heat Shock Protein Genes”, Harran Univ Vet Fak Derg, c. 13, sy. 2, ss. 84–92, 2024, doi: 10.31196/huvfd.1451671.
ISNAD Ekren Aşıcı, Gamze Sevri vd. “Effect of Propolis Applied to Goat Kids at Weaning Period on Heat Shock Protein Genes”. Harran Üniversitesi Veteriner Fakültesi Dergisi 13/2 (Aralık 2024), 84-92. https://doi.org/10.31196/huvfd.1451671.
JAMA Ekren Aşıcı GS, Çağlı A, Çoğan H, Kargın Kıral F, Yılmaz M. Effect of Propolis Applied to Goat Kids at Weaning Period on Heat Shock Protein Genes. Harran Univ Vet Fak Derg. 2024;13:84–92.
MLA Ekren Aşıcı, Gamze Sevri vd. “Effect of Propolis Applied to Goat Kids at Weaning Period on Heat Shock Protein Genes”. Harran Üniversitesi Veteriner Fakültesi Dergisi, c. 13, sy. 2, 2024, ss. 84-92, doi:10.31196/huvfd.1451671.
Vancouver Ekren Aşıcı GS, Çağlı A, Çoğan H, Kargın Kıral F, Yılmaz M. Effect of Propolis Applied to Goat Kids at Weaning Period on Heat Shock Protein Genes. Harran Univ Vet Fak Derg. 2024;13(2):84-92.