Mangan süperoksit dismutaz Ala16Val gen polimorfizmlerinin koroner arter olaylarında koruyucu rollerinin araştırılması

Yıl 2015, Cilt: 1 Sayı: 3, 134 - 144, 07.12.2015

Hale Atmaca Meliha Koldemir Gündüz Penbe Çağatay Mehtap Çevik Belgin Süsleyici Duman

Öz

Amaç: Araştırmamızın amacı, mangan süperoksit dismutaz (MnSOD) genine ait Ala16Val polimorfizminin koroner arter hastalığı (KAH) olan kişilerdeki genotip sıklıklarını belirlemek ve genotip-kalp damar hastalığı ve genotip-biyokimyasal parametreler ve serum MnSOD aktivitesi ile etkileşimin değerlendirilmesidir.

Gereç ve yöntemler: MnSOD geni Ala16Val genotipleri kantitatif polimeraz zincir reaksiyonu yöntemi ile saptandı. Serum MnSOD aktivitesi ELİSA yöntemi ile belirlendi.

Bulgular: MnSOD geni Ala16Val polimorfizmi için genotip sıklıkları KAH grubunda C/C (Homozigot yabanıl tip), C/T (Heterozigot), T/T (Homozigot polimorfik tip) genotipler için sırasıyla %20.9, %41.9, %37.2 ve KAH olmayan grupta %15.5, %51.7, %32.8 olarak tespit edildi. Tüm çalışma grubundaki mitokondriyal MnSOD aktiviteleri, MnSOD geni Ala16Val genotiplerine göre karşılaştırıldığında CC genotip taşıyıcılarının en yüksek, TT genotip taşıyıcılarının en düşük MnSOD aktivitesine sahip olduğu belirlendi. Koroner arter hastalığı bulunmayan hastalarda MnSOD Ala16Val polimorfizminin yağsız vücut kütlesi (p=0.01) ve yağ kütlesi (p=0.05) üzerine anlamlı etkileri olduğu saptandı. Ala16Val polimorfizminin heterozigot genotipe sahip KAH hastalarında yağdan zengin gıdalarla beslenme alışkanlıkları diğer genotiplere kıyasla daha düşük iken, homozigot polimorfik genotipe sahip KAH hastalarının diğer genotiplere kıyasla proteinden fakir beslenme alışkanlıklarını benimsedikleri görüldü.

Sonuç: Tüm çalışma grubu değerlendirildiğinde, MnSOD Ala16Val genotipleri ile beslenme alışkanlıkları arasında anlamlı bir ilişki bulunurken yağsız vücut kütlesi üzerinde etkili bulunmamıştır.

Anahtar Kelimeler

Ala16Val polimorfizmi, koroner arter hastalığı, MnSOD geni

Kaynakça

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