Akuakültürde Tespit Edilen Fekal Koliform Kökenli Antimikrobiyal Direnç Genleri

Öz

Akarsu ve denizler üzerinde faaliyet gösteren balık çiftliklerinin son yıllarda sayıları artmış olup bu çiftliklerde kullanılan atık sular arıtılmadan direkt doğaya deşarj edilmektedir. Balık çiftlikleri genel olarak su kaynaklarının atık su, kentsel su ve çiftlik suları ile karıştırılıp karıştırılmadığına bakılmaksızın akarsu ya da barajlar üzerinde kurulmakta ve bu sular sucul canlılar ve karasal hayvanlar tarafından kullanılmaktadır. Akarsulara kentsel atık suların karışması ile balık çiftliklerde koliform kontaminasyonuna neden olmaktadır.

Bu çalışma sularda kirlilik göstergesi olan Enterobacter clocae ve Enterobacter asburiae gibi fekal koliformların ve antimikrobiyel direnç genlerinin tespit edilerek fekal koliformların akuatik ortamları kontaminasyonunu engellenmesi amacıyla yapılmıştır.

Çalışma kapsamında toplam 6 adet Enterobacter clocae ve Enterobacter asburiae 16S rRNA ve gyrB gen bölgesi de dahil olmak üzere biyokimyasal ve moleküler yöntemlerle identifiye edilmiştir. Daha sonra gen bölgesinden elde edilen sonuçlar, Gen Bank veri tabanında karşılaştırılmış ve veri tabanında kayıtlı olan izolatlarla karşılaştırılmıştır.

Anahtar Kelimeler

• Akuakültür,Antimikrobiyal Direnç Genleri,Enterobacter

Kaynakça

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