Akuakültürde Tespit Edilen Fekal Koliform Kökenli Antimikrobiyal Direnç Genleri

Yıl 2018, Cilt: 3 Sayı: 1, 22 - 26, 15.08.2018

Özcan Yavaş , Muhammed Duman , İzzet Burçin Satıcıoğlu Ebru Garip Feza Karaer , Soner Altun

Öz

Akarsu ve denizler üzerinde
faaliyet gösteren balık çiftliklerinin son yıllarda sayıları artmış olup bu
çiftliklerde kullanılan atık sular arıtılmadan direkt doğaya deşarj
edilmektedir. Balık çiftlikleri genel olarak su kaynaklarının atık su, kentsel
su ve çiftlik suları ile karıştırılıp karıştırılmadığına bakılmaksızın akarsu
ya da barajlar üzerinde kurulmakta ve bu sular sucul canlılar ve karasal
hayvanlar tarafından kullanılmaktadır. Akarsulara kentsel atık suların
karışması ile balık çiftliklerde koliform kontaminasyonuna neden olmaktadır.

Bu çalışma sularda kirlilik
göstergesi olan Enterobacter clocae ve Enterobacter asburiae gibi fekal
koliformların ve antimikrobiyel direnç genlerinin tespit edilerek fekal
koliformların akuatik ortamları kontaminasyonunu engellenmesi amacıyla
yapılmıştır.

Çalışma kapsamında toplam 6
adet Enterobacter clocae ve Enterobacter asburiae 16S rRNA ve gyrB
gen bölgesi de dahil olmak üzere biyokimyasal ve moleküler yöntemlerle
identifiye edilmiştir. Daha sonra gen bölgesinden elde edilen sonuçlar, Gen
Bank veri tabanında karşılaştırılmış ve veri tabanında kayıtlı olan izolatlarla
karşılaştırılmıştır.

Anahtar Kelimeler

Akuakültür, Antimikrobiyal Direnç Genleri, Enterobacter

Kaynakça

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