ICP-MS ile Lucilia Sericata (Meigen, 1826) [Diptera: Calliphoridae] Larvalarından Arsenik ve Kadmiyum İntoksikasyon Olgularının Tahmini

Öz

Amaç: Bu çalışmada, kontamine karaciğer dokusu ile beslenen Lucilia sericata (Meigen, 1826) larvalarında arsenik (As) ve kadmiyum (Cd) tespiti için İndüktif Eşleşmiş Plazma-Kütle Spektrometrisi (ICP-MS) temelli bir analitik yöntemin geliştirilmesi ve valide edilmesi ile beslenme ortamı ve larvalardaki element konsantrasyonları arasındaki ilişkinin değerlendirilmesi amaçlanmıştır.

Yöntem: Farklı As ve Cd konsantrasyonları ile kontamine edilen tavuk karaciğeri temelli beslenme ortamlarına yaklaşık 100 L. sericata yumurtası inoküle edilmiş ve larvalar üçüncü evreye ulaşıncaya kadar üç gün inkübe edilmiştir. Larvalar nitrik asit ve hidrojen peroksit ile parçalanarak ICP-MS ile analiz edilmiştir. Kör larvalardan matriks uyumlu kalibrasyon eğrileri hazırlanmış; yöntem performansı doğrusallık, LOD, LOQ, geri kazanım ile gün içi ve günler arası yeniden üretilebilirlik açısından değerlendirilmiştir.

Bulgular: Matriks uyumlu kalibrasyon eğrileri her iki element için 1–20 ng/mL aralığında doğrusal bulunmuştur. Geri kazanım değerleri As için %94,33–107,38, Cd için %97,98–108,16 arasında belirlenmiştir. Gün içi bağıl standart sapma değerleri As için %3,46–8,51, Cd için %2,22–10,36 bulunmuştur. As ve Cd’ye maruz kalan larvaların ortalama uzunluklarının kör gruba göre daha düşük olduğu gözlenmiştir. Beslenme ortamı ve larvalardaki element konsantrasyonları arasındaki korelasyon katsayıları As için 0,677, Cd için 0,902 olarak hesaplanmıştır.

Sonuç: Geliştirilen ICP-MS yöntemi, L. sericata larvalarında As ve Cd’nin doğru ve tekrarlanabilir şekilde tespit edilmesini sağlamıştır. Bulgular, larval element analizinin özellikle geleneksel biyolojik matrikslerin bulunmadığı şüpheli post-mortem zehirlenme olgularında adli araştırmalara katkı sağlayabilecek toksikolojik bilgi sunabileceğini göstermektedir.

Anahtar Kelimeler

• Adli entomoloji
• entomotoksikoloji
• Lucilia sericata
• ICP-MS
• arsenik
• kadmiyum

Destekleyen Kurum

İstanbul Üniversitesi-Cerrahpaşa Bilimsel Araştırma Projeleri Birimi, Türkiye

Proje Numarası

FYL-2020-3450

Etik Beyan

Bu çalışmada deneysel model olarak omurgasız bir tür olan Lucilia sericata larvaları kullanılmıştır. İstanbul Üniversitesi-Cerrahpaşa Hayvan Deneyleri Yerel Etik Kurulu yönergelerine göre omurgasız türlerle yapılan çalışmalar etik kurul onayı gerektirmemektedir. Beslenme ortamı olarak kullanılan tavuk karaciğeri yerel bir perakende marketten ticari olarak temin edilmiş olup, bu çalışma için özel olarak hiçbir hayvan sakrifiye edilmemiştir. Çalışma insan katılımcı içermediğinden bilgilendirilmiş gönüllü onamı uygulanabilir değildir.

Teşekkür

Bu çalışma, FYL-2020-3450 proje numarası ile İstanbul Üniversitesi-Cerrahpaşa Bilimsel Araştırma Projeleri Birimi tarafından desteklenmiştir.

Prediction of Arsenic and Cadmium Intoxication Cases from Lucilia Sericata (Meigen, 1826) [Diptera: Calliphoridae] Larvae by ICP-MS

Öz

Aim: This study aimed to develop and validate an Inductively Coupled Plasma-Mass Spectrometry (ICP-MS)-based analytical method for detecting arsenic (As) and cadmium (Cd) in Lucilia sericata (Meigen, 1826) larvae fed on contaminated liver tissue and to evaluate the relationship between element concentrations in the feeding media and larvae.

Method: Chicken liver-based media spiked with different As and Cd concentrations were inoculated with approximately 100 L. sericata eggs and incubated for three days until the third instar stage. Larvae were digested with nitric acid and hydrogen peroxide, then analyzed by ICP-MS. Matrix-matched calibration curves were prepared from blank larvae, and method performance was assessed for linearity, limit of detection (LOD), limit of quantification (LOQ), recovery, and intra- and inter-day reproducibility.

Results: Matrix-matched calibration curves were linear in the range of 1–20 ng/mL for both elements. Recovery values ranged from 94.33% to 107.38% for As and from 97.98% to 108.16% for Cd. Intra-day relative standard deviation values were 3.46–8.51% for As and 2.22–10.36% for Cd. Larvae exposed to As and Cd showed shorter average lengths than the blank group. The correlation coefficients between element concentrations in the feeding media and larvae were 0.677 for As and 0.902 for Cd.

Conclusion: The developed ICP-MS method provided accurate and reproducible detection of As and Cd in L. sericata larvae. These findings suggest that larval elemental analysis may provide useful toxicological information in forensic investigations, particularly when conventional biological matrices are unavailable in suspected post-mortem poisoning cases.

Anahtar Kelimeler

• Forensic entomology
• entomotoxicology
• Lucilia sericata
• ICP-MS
• arsenic
• cadmium

Destekleyen Kurum

Scientific Research Projects Unit of Istanbul University-Cerrahpaşa, Türkiye

Proje Numarası

FYL-2020-3450

Etik Beyan

The experimental model used in this study involved larvae of Lucilia sericata, an invertebrate species. According to the guidelines of the Istanbul University-Cerrahpasa Animal Experiments Local Ethics Committee, studies involving invertebrates do not require ethical approval. Chicken liver used as feeding media was commercially obtained from a local retail market, and no animals were sacrificed specifically for this study. Since the study did not involve human participants, informed consent was not applicable.

Teşekkür

This study was supported by the Scientific Research Projects Unit of Istanbul University-Cerrahpasa, Türkiye, under project number FYL-2020-3450.

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