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PREDICTION PERFORMANCE OF DECISION TREE INDUCERS ON AUGMENTED BACILLUS CEREUS GROWTH DATA

Yıl 2025, Cilt: 9 Sayı: 2, 352 - 362, 30.08.2025
https://doi.org/10.46519/ij3dptdi.1726410

Öz

Accurate prediction of microbial growth is of great importance in critical areas such as food safety and environmental sciences. In this study, a hybrid of mathematical methods and machine learning-based approaches are used to model the growth dynamics of foodborne pathogen Bacillus cereus. Since the use of mathematical models alone does not sufficiently cover the non-linear data structure of bacterial systems, better results are obtained when hybrids are used together with machine learning methods. We examine the results of five different tree-based models for predicting the growth of Bacillus cereus, namely Fine Tree, Medium Tree, Coarse Tree, Ensemble Boosted Trees and Ensemble Bagged Trees. We evaluate each model with performance metrics such as Root Mean Square Error (RMSE), Mean Square Error (MSE), R² and Mean Absolute Error (MAE). The results show that the Ensemble Bagged Trees model performs the best, with a validation RMSE of 0.0094 and an R² value of 0.9995. Also, the Fine Tree model has an R² value of 0.9990. In general, ensemble methods offer significant advantages in prediction accuracy.

Kaynakça

  • 1. Rizki, W.M., Ratih, D.H., Harsi, D.K., “Comparison of Predictive Growth Models for Bacillus Cereus in Cooked and Fried Rice During Storage”, The Annals of the University Dunarea De Jos of Galati. Fascicle VI - Food Technology, Vol. 46, Issue 2, Pages 89-103, 2022.
  • 2. Ellouze, M., Buss Da Silva, N., Rouzeau-Szynalski, K., Coisne, L., Cantergiani, F., Baranyi, J., “Modeling Bacillus cereus Growth and Cereulide Formation in Cereal, Dairy, Meat, Vegetable-Based Food and Culture Medium”, Frontiers in Microbiology, Vol. 12, Article 639546, 2021.
  • 3. Buss da Silva, N., Baranyi, J., Carciofi, B.A.M., Ellouze, M., “From Culture-Medium-Based Models to Applications to Food: Predicting the Growth of B. cereus in Reconstituted Infant Formulae”, Frontiers in Microbiology, Vol. 8, Article 1799, 2017.
  • 4. Bursová, Š., Haruštiaková, D., Necidová, L., Krobotová, E., Mlejnková, Z., Tkáč, M., Stojanová, K., Golian, J., “Evaluation of Bacillus cereus growth in cooked rice”, The Journal of Microbiology, Biotechnology and Food Sciences, Vol. 14, Issue 1, Article e10985, 2024.
  • 5. Ahmad, M.M., Azoddein, A.M., Olalere, O.A., Isa, S., “A Predictive Batch Culture Growth and Biosynthesis for Bacillus cereus (ATCC 14579) using Response Surface Methodology”, Journal of Environmental Bioremediation and Toxicology, Vol. 5, Issue 2, Pages 40-45, 2022.
  • 6. Juneja, V.K., Golden, C.E., Mishra, A., Harrison, M., Mohr, T.B., “Predictive model for growth of Bacillus cereus at temperatures applicable to cooling of cooked pasta”, Journal of Food Science, Vol. 84, Issue 3, Pages 590-598, 2019.
  • 7. Valik, L., Görner, F., Laukova, D., “Growth dynamics of Bacillus cereus and shelf-life of pasteurized milk”, Czech Journal of Food Sciences, Vol. 21, Issue 6, Pages 195-202, 2003.
  • 8. Choi, M.-S., Kim, J.Y., Jeon, E.B., Park, S.Y., “Predictive growth models of Bacillus cereus on dried laver Pyropia pseudolinearis as a function of storage temperature”, Korean Journal of Fisheries and Aquatic Sciences, Vol. 53, Issue 5, Pages 699-706, 2020.
  • 9. Jiang, Y.N., Luo, J., Huang, D., Liu, Y., Li, D., “Machine learning advances in microbiology: A review of methods and applications”, Frontiers in Microbiology, Vol. 13, Article 925454, 2022.
  • 10. Romana, A.S., “A Comparative Study of Different Machine Learning Algorithms for Disease Prediction”, International Journal of Advanced Research in Computer Science and Software Engineering, Vol. 7, Issue 7, Pages 172, 2017.
  • 11. Atolia, E., Cesar, S., Arjes, H.A., Rajendram, M., Shi, H., Knapp, B.D., Khare, S., Aranda-Díaz, A., Lenski, R.E., Huang, K.C., “Environmental and physiological factors affecting high-throughput measurements of bacterial growth”, mBio, Vol. 11, Issue 5, Article e01378-20, 2020.
  • 12. Eckart, L., Eckart, S., Enke, M., “A brief comparative study of the potentialities and limitations of machine-learning algorithms and statistical techniques”, E3S Web of Conferences, Vol. 266, Article 02001, 2021.
  • 13. Weller, D., Love, T., Wiedmann, M., “Interpretability versus accuracy: A comparison of machine learning models built using different algorithms, performance measures, and features to predict E. coli levels in agricultural water”, Frontiers in Artificial Intelligence, Vol. 4, Article 628441, 2021.
  • 14. Chorin, E., Thuault, D., Cléret, J.J., Bourgeois, C.M., “Modelling Bacillus cereus growth”, International Journal of Food Microbiology, Vol. 38, Issues 2-3, Pages 229-234, 1997. 15. Ince, V., Bader-El-Den, M., Arabikhan, F., Sari, O.F., “Machine Learning for Bacterial Growth Prediction and Examination of Bacterial Impact on Growth”, Proceedings of the 2024 IEEE 12th International Conference on Intelligent Systems (IS), Pages 1-6, 2024.
  • 16. Kowalik, J., Lobacz, A., Zulewska, J., Dec, B., “Analysis and mathematical modelling of the behaviour of Escherichia coli in the mascarpone cheese during cold storage”, International Journal of Food Science and Technology, Vol. 53, Issue 6, Pages 1541–1548, 2018.
  • 17. Martinez-Rios, V., Gkogka, E., Dalgaard, P., “Predicting growth of Listeria monocytogenes at dynamic conditions during manufacturing, ripening and storage of cheese - Evaluation and application of models”, Food Microbiology, Vol. 92, Pages 1–12, 2020.
  • 18. Stavropoulou, E., Bezirtzoglou, E., “Predictive modeling of microbial behavior in food”, Foods, Vol. 8, Issue 12, Pages 1-16, 2019.
  • 19. Baranyi, J., Buss da Silva, N., Ellouze, M., “Rethinking tertiary models: relationships between growth parameters of Bacillus cereus strains”, Frontiers in Food Microbiology, Vol. 8, Article 1890, 2017.

PREDICTION PERFORMANCE OF DECISION TREE INDUCERS ON AUGMENTED BACILLUS CEREUS GROWTH DATA

Yıl 2025, Cilt: 9 Sayı: 2, 352 - 362, 30.08.2025
https://doi.org/10.46519/ij3dptdi.1726410

Öz

Accurate prediction of microbial growth is of great importance in critical areas such as food safety and environmental sciences. In this study, a hybrid of mathematical methods and machine learning-based approaches are used to model the growth dynamics of foodborne pathogen Bacillus cereus. Since the use of mathematical models alone does not sufficiently cover the non-linear data structure of bacterial systems, better results are obtained when hybrids are used together with machine learning methods. We examine the results of five different tree-based models for predicting the growth of Bacillus cereus, namely Fine Tree, Medium Tree, Coarse Tree, Ensemble Boosted Trees and Ensemble Bagged Trees. We evaluate each model with performance metrics such as Root Mean Square Error (RMSE), Mean Square Error (MSE), R² and Mean Absolute Error (MAE). The results show that the Ensemble Bagged Trees model performs the best, with a validation RMSE of 0.0094 and an R² value of 0.9995. Also, the Fine Tree model has an R² value of 0.9990. In general, ensemble methods offer significant advantages in prediction accuracy.

Kaynakça

  • 1. Rizki, W.M., Ratih, D.H., Harsi, D.K., “Comparison of Predictive Growth Models for Bacillus Cereus in Cooked and Fried Rice During Storage”, The Annals of the University Dunarea De Jos of Galati. Fascicle VI - Food Technology, Vol. 46, Issue 2, Pages 89-103, 2022.
  • 2. Ellouze, M., Buss Da Silva, N., Rouzeau-Szynalski, K., Coisne, L., Cantergiani, F., Baranyi, J., “Modeling Bacillus cereus Growth and Cereulide Formation in Cereal, Dairy, Meat, Vegetable-Based Food and Culture Medium”, Frontiers in Microbiology, Vol. 12, Article 639546, 2021.
  • 3. Buss da Silva, N., Baranyi, J., Carciofi, B.A.M., Ellouze, M., “From Culture-Medium-Based Models to Applications to Food: Predicting the Growth of B. cereus in Reconstituted Infant Formulae”, Frontiers in Microbiology, Vol. 8, Article 1799, 2017.
  • 4. Bursová, Š., Haruštiaková, D., Necidová, L., Krobotová, E., Mlejnková, Z., Tkáč, M., Stojanová, K., Golian, J., “Evaluation of Bacillus cereus growth in cooked rice”, The Journal of Microbiology, Biotechnology and Food Sciences, Vol. 14, Issue 1, Article e10985, 2024.
  • 5. Ahmad, M.M., Azoddein, A.M., Olalere, O.A., Isa, S., “A Predictive Batch Culture Growth and Biosynthesis for Bacillus cereus (ATCC 14579) using Response Surface Methodology”, Journal of Environmental Bioremediation and Toxicology, Vol. 5, Issue 2, Pages 40-45, 2022.
  • 6. Juneja, V.K., Golden, C.E., Mishra, A., Harrison, M., Mohr, T.B., “Predictive model for growth of Bacillus cereus at temperatures applicable to cooling of cooked pasta”, Journal of Food Science, Vol. 84, Issue 3, Pages 590-598, 2019.
  • 7. Valik, L., Görner, F., Laukova, D., “Growth dynamics of Bacillus cereus and shelf-life of pasteurized milk”, Czech Journal of Food Sciences, Vol. 21, Issue 6, Pages 195-202, 2003.
  • 8. Choi, M.-S., Kim, J.Y., Jeon, E.B., Park, S.Y., “Predictive growth models of Bacillus cereus on dried laver Pyropia pseudolinearis as a function of storage temperature”, Korean Journal of Fisheries and Aquatic Sciences, Vol. 53, Issue 5, Pages 699-706, 2020.
  • 9. Jiang, Y.N., Luo, J., Huang, D., Liu, Y., Li, D., “Machine learning advances in microbiology: A review of methods and applications”, Frontiers in Microbiology, Vol. 13, Article 925454, 2022.
  • 10. Romana, A.S., “A Comparative Study of Different Machine Learning Algorithms for Disease Prediction”, International Journal of Advanced Research in Computer Science and Software Engineering, Vol. 7, Issue 7, Pages 172, 2017.
  • 11. Atolia, E., Cesar, S., Arjes, H.A., Rajendram, M., Shi, H., Knapp, B.D., Khare, S., Aranda-Díaz, A., Lenski, R.E., Huang, K.C., “Environmental and physiological factors affecting high-throughput measurements of bacterial growth”, mBio, Vol. 11, Issue 5, Article e01378-20, 2020.
  • 12. Eckart, L., Eckart, S., Enke, M., “A brief comparative study of the potentialities and limitations of machine-learning algorithms and statistical techniques”, E3S Web of Conferences, Vol. 266, Article 02001, 2021.
  • 13. Weller, D., Love, T., Wiedmann, M., “Interpretability versus accuracy: A comparison of machine learning models built using different algorithms, performance measures, and features to predict E. coli levels in agricultural water”, Frontiers in Artificial Intelligence, Vol. 4, Article 628441, 2021.
  • 14. Chorin, E., Thuault, D., Cléret, J.J., Bourgeois, C.M., “Modelling Bacillus cereus growth”, International Journal of Food Microbiology, Vol. 38, Issues 2-3, Pages 229-234, 1997. 15. Ince, V., Bader-El-Den, M., Arabikhan, F., Sari, O.F., “Machine Learning for Bacterial Growth Prediction and Examination of Bacterial Impact on Growth”, Proceedings of the 2024 IEEE 12th International Conference on Intelligent Systems (IS), Pages 1-6, 2024.
  • 16. Kowalik, J., Lobacz, A., Zulewska, J., Dec, B., “Analysis and mathematical modelling of the behaviour of Escherichia coli in the mascarpone cheese during cold storage”, International Journal of Food Science and Technology, Vol. 53, Issue 6, Pages 1541–1548, 2018.
  • 17. Martinez-Rios, V., Gkogka, E., Dalgaard, P., “Predicting growth of Listeria monocytogenes at dynamic conditions during manufacturing, ripening and storage of cheese - Evaluation and application of models”, Food Microbiology, Vol. 92, Pages 1–12, 2020.
  • 18. Stavropoulou, E., Bezirtzoglou, E., “Predictive modeling of microbial behavior in food”, Foods, Vol. 8, Issue 12, Pages 1-16, 2019.
  • 19. Baranyi, J., Buss da Silva, N., Ellouze, M., “Rethinking tertiary models: relationships between growth parameters of Bacillus cereus strains”, Frontiers in Food Microbiology, Vol. 8, Article 1890, 2017.
Toplam 18 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Yazılım Mühendisliği (Diğer)
Bölüm Araştırma Makalesi
Yazarlar

Hamit Armağan 0000-0002-8948-1546

Yayımlanma Tarihi 30 Ağustos 2025
Gönderilme Tarihi 25 Haziran 2025
Kabul Tarihi 15 Ağustos 2025
Yayımlandığı Sayı Yıl 2025 Cilt: 9 Sayı: 2

Kaynak Göster

APA Armağan, H. (2025). PREDICTION PERFORMANCE OF DECISION TREE INDUCERS ON AUGMENTED BACILLUS CEREUS GROWTH DATA. International Journal of 3D Printing Technologies and Digital Industry, 9(2), 352-362. https://doi.org/10.46519/ij3dptdi.1726410
AMA Armağan H. PREDICTION PERFORMANCE OF DECISION TREE INDUCERS ON AUGMENTED BACILLUS CEREUS GROWTH DATA. IJ3DPTDI. Ağustos 2025;9(2):352-362. doi:10.46519/ij3dptdi.1726410
Chicago Armağan, Hamit. “PREDICTION PERFORMANCE OF DECISION TREE INDUCERS ON AUGMENTED BACILLUS CEREUS GROWTH DATA”. International Journal of 3D Printing Technologies and Digital Industry 9, sy. 2 (Ağustos 2025): 352-62. https://doi.org/10.46519/ij3dptdi.1726410.
EndNote Armağan H (01 Ağustos 2025) PREDICTION PERFORMANCE OF DECISION TREE INDUCERS ON AUGMENTED BACILLUS CEREUS GROWTH DATA. International Journal of 3D Printing Technologies and Digital Industry 9 2 352–362.
IEEE H. Armağan, “PREDICTION PERFORMANCE OF DECISION TREE INDUCERS ON AUGMENTED BACILLUS CEREUS GROWTH DATA”, IJ3DPTDI, c. 9, sy. 2, ss. 352–362, 2025, doi: 10.46519/ij3dptdi.1726410.
ISNAD Armağan, Hamit. “PREDICTION PERFORMANCE OF DECISION TREE INDUCERS ON AUGMENTED BACILLUS CEREUS GROWTH DATA”. International Journal of 3D Printing Technologies and Digital Industry 9/2 (Ağustos2025), 352-362. https://doi.org/10.46519/ij3dptdi.1726410.
JAMA Armağan H. PREDICTION PERFORMANCE OF DECISION TREE INDUCERS ON AUGMENTED BACILLUS CEREUS GROWTH DATA. IJ3DPTDI. 2025;9:352–362.
MLA Armağan, Hamit. “PREDICTION PERFORMANCE OF DECISION TREE INDUCERS ON AUGMENTED BACILLUS CEREUS GROWTH DATA”. International Journal of 3D Printing Technologies and Digital Industry, c. 9, sy. 2, 2025, ss. 352-6, doi:10.46519/ij3dptdi.1726410.
Vancouver Armağan H. PREDICTION PERFORMANCE OF DECISION TREE INDUCERS ON AUGMENTED BACILLUS CEREUS GROWTH DATA. IJ3DPTDI. 2025;9(2):352-6.

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