Virus diseases of lettuce in ankara province

Yıl 2019, Cilt: 3 Sayı: 2, 202 - 206, 23.12.2019

Filiz Ertunç , Filiz Randa Zelyut

Öz

Lettuce is one
of the widely grown vegetable in Ankara province of Turkey and virus diseases
cause great yield loss.  Surveys were conducted to five
different districts of Ankara during two years, 2014-2015 and a total of 544
samples were collected, and all were tested with the DAS-ELISA against Mirafiori lettuce big vein virus
(MiLBVV), Lettuce mosaic virus (LMV),
Cucumber mosaic virus (CMV), Tomato spotted wilt virus (TSVW)
antisera. LMV was the predominant and unique infection detected in 2014 and was
present in the 65 of the specimen collected but  in 2015, 39 MiLBVV, 25 LMV infected isolates
were detected. TSWV and CMV were not present in the research area. RT-PCR tests
were performed to determine the coat protein gene regions of those viruses.
Amplicons were obtained at 800 bp, 469 bp and 296 bp of LMV, MiLBVV and LBVaV,
respectively. Lettuce big vein-associated virus (LBVaV) were present in the 5
out of a total of 6 samples, as mixed infected with MiLBVV. Microscopic
observations were performed to determine the presence of Olpidium spp. in the roots which is the vector of the viruses
belong to Lettuce Big Vein diseases (LBV), resting spores were observed in 146
of the investigated root samples. These results indicate that LMV and LBV diseases causing viruses are the most common
endemic viral pathogens in Ankara's lettuce cultivation areas and the vector Olpidium spp. was present in the
research area.

Anahtar Kelimeler

Ankara, lettuce viruses, DAS-ELISA, olpidium spp

Kaynakça

• Clark, M. F. and Adams, A. N. (1977) Characteristics of the micro plate method of enzyme linked immunosorbent assay for the detection of plant viruses. Journal of General Virology, 34:475-48. Döken, M. T., Demirci, E. and Açıkgöz, S. (1994). Morphology of Olpidium brassicae (Wor.) Dang. and its transmission of big-vein virus to lettuce. Journal of Turkish Phytopathology, 23(3): 133-142.Erkan, S. and Schlösser, E. (1985) Virus diseases on lettuce in Turkey. Journal of Plant Diseases and Protection, 92 (2), 127-131. Fidan, Ü. and Türkoğlu, T. (1988) Ege bölgesi marul bitkilerinde görülen virüs hastalıkları üzerinde ön çalışmalar. Bitki Koruma Bülteni, 28 (1-2):43-56. German-Retana, S., Walter, J. and Gall, O. L. (2008) Lettuce mosaic virus: from pathogen diversity to host interactors. Molecular Plant Pathology, 9:127-136.Herrera-Vasquez, J. A., Cebria, M.C., Ana Alfaro-Fernandez, A., Cordoba-Selles, M. and Jorda, C. (2009) Multiplex PCR assay for the simultaneous detection and differentiation of Olpidium bornovanus, O. brassicae, and O. virulentus. Mycological Research, 113, 602–610.
• Kamberoğlu, M. A. and Alan, B. (2011) Occurrence of Tomato spotted wilt virus in lettuce in Çukurova Region of Turkey. International Journal of Agriculture & Biology, 13: 431–434.
• Karanfil, A., Çevik, B. and Korkmaz, S. (2018) Detection of Lettuce mosaic virus infection in South Marmara Region of Turkey and coat protein gene characterization. Zemdirbyste-Agriculture, 105(4): 363-368.
• King, A. M. Q., Adams, M. J., Carstens, E. B. and Lefkowitz, E. J. (2012) Virus taxonomy classification and nomenclature of viruses ninth report of the international committee on taxonomy of viruses. Elsevier, UK, pp. 1072-1077.
• Maccarone, L. D. (2013) Relationships between the pathogen Olpidium virulentus and viruses associated with lettuce big-vein disease. Plant Diseases, 97: 700–707.
• Moreno, A. and Fereres, A. (2012) Virus diseases in lettuce in the Mediterranean Basin. Advances Virus Research, 84, 247–288.
• Navarro, J. A., Botella, F. A., Maruhenda, A., Sastre, P., Sanchez-Pina, M. A. and Pallas, V. (2004) Comparative infection progress analysis of Lettuce big-veinvirus and Mirafiori lettuce virus in lettuce crops by developed molecular diagnosis techniques. Phytopathology, 94, 470-477.
• Ozalp, O. (1964) İzmir'de sebzeler de görülen virüs hastalıkları. Bitki Koruma Bülteni, 4(1): 18-25.
• Palukaitis, P., Roossinck, M. J., Dietzgen, R. G. and Francki, R. I. B. (1992) Cucumber mosaic virus. Advances Virus Research, 41, 281–348.
• Pavan, M. A., Krause-Sakate, R., da Silva, N., Zerbini, F. M. and Le Gall, O. (2008) Virus diseases of lettuce in Brazil. Plant viruses, 2: 35-41.
• Randa Zelyüt, F. and Ertunç, F. (2018) Molecular characterization of lettuce mosaic virus isolates in Ankara province. International Journal of Agriculture Innovations and Research, 6(5):239-245.
• Sasaya, T., Fujii, H., Ishikawa, K. and Koganezawa, H. (2008) Further evidence of Mirafiori lettuce big-vein virus but not of Lettuce big-vein associated virus with big-vein disease in lettuce. Phytopathology, 98:464- 468.
• Tekinel, N., Dolar, M. S., Sağsöz, S. and Salcan, Y. (1969) Mersin Bölgesinde Ekonomik Bakımdan Önemli Bazı Sebzelerin Virüsleri Üzerinde Araştırmalar. Bitki Koruma Bülteni, 9:1, 37-49.
• Tomlinson, J. A. (1970) Lettuce mosaic virus. CMI/AAB Descriptions of Plant Viruses, no:9.
• TUIK, (2018) Bitkisel üretim istatistikleri. Retrieved in December, 10, 2018 from https://biruni.tuik.gov.tr/medas/?kn=92&locale=tr
• Verbeek, M., Dullemans, A. M., van Bekkum, P. J. and van der Vlugt, R. A. A. (2013) Evidence for Lettuce big-vein associated virus as the causal agent of a syndrome of necrotic rings and spots in lettuce. Plant Pathology, 62: 444- 451.
• Verbeek, M., Dullemans, A.M., Raaij, H.G.,Verhoeven, J.T.J. and van der Vlugt, R.A.A. (2014) Lettuce necrotic leaf curl virus, a new plant virus infecting lettuce and aproposed member of the genus Torradovirus. Archives of Virology, 159:801–805.Westerlund, F. V., Campbell, R. N. and Grogan, R. G. (1978) Effect of temperatue on transmission, translocation, and persistence of the lettuce big-vein agent and big-vein symptom expression. Phyopathology, 921-926. Whitfield, A. E., Ullman, D. E. and German, T. L. (2005) Tospovirus–thrips interactions. Annual Review of Phytopathology, 43: 459–489.
• Zerbini, F. M., Koike, S. T. and Gilbertson, R. L. (1995) Biological and molecular characterization of Lettuce mosaic potyvirus isolates from Salinas Valley of California. Phytopathology, 8: 746-752.