Moleküler Markerlar Kullanarak Çarliston ve Dolmalık Biber Hatlarında Genetik Çeşitliliğin Belirlenmesi

Öz

Hibrit çeşit ıslahında başarının sağlanması; genetik çeşitliliği yüksek bitki kaynağına, melezlenecek hatların homozigot olmasına ve melezlenecek hatların genetik olarak birbirinden uzak olmasına bağlıdır. Bu çalışmada, biber (Capsicum annum L.) saf hatları arasındaki genetik uzaklığın belirlenmesi ve ıslah programının etkinliğinin arttırılması amaçlanmıştır. Çalışmada 44 adet dolmalık ve 35 adet çarliston biber saf hatları arasındaki genetik uzaklık, SRAP (Sequence Related Amplified Polymorphism) markerı ile araştırılmıştır. Dendrogramlar UPGMA (unweighted pair-group method of mathematical averages method) yöntemine göre oluşturulmuştur. Test edilen 71 primer kombinasyonundan, çarliston biber hatlarında 50 kombinasyon polimorfizm göstermiş ve toplamda 123 adet polimorfik bant elde edilmiştir. Dolmalık biber hatlarında 24 SRAP primer kombinasyonu test edilmiş ve 15 kombinasyondan 25 polimorfik bant elde edilmiştir. UPGMA küme analizine göre biber hatları dolmalık biber ve çarliston biber olarak gruplara ayrılmıştır. Çarliston biber hatları arasındaki genetik benzerlik 0.62 ile 0.98 arasında değişirken, dolmalık biber hatları arasındaki genetik benzerlik 0.54 ile 1.00 arasında değişmiştir. Sonuç olarak, SRAP markerlarının biber saf hatlarının genetik uzaklıklarının belirlenmesinde başarılı bir şekilde kullanılarak ıslah programına yardımcı olacağı ifade edilebilir.

Anahtar Kelimeler

• Biber
• genetik akrabalık
• SRAP
• polimorfizm

Determination of Genetic Diversity in Banana and Bell Pepper Lines Using Molecular Markers

Abstract

Successful hybrid cultivar breeding is depend on the high genetic diversity of the plant sources, as well as the homozygous and genetically distant lines requiring hybridization. The aim of this study is to determine the genetic distance between the inbred lines of pepper (Capsicum annuum L.) in order to increase efficacy of the breeding program. In this study, the genetic distances between the inbred lines of 44 bell peppers and 35 banana peppers were investigated using the SRAP (Sequence Related Amplified Polymorphism) markers. Based on pattern scores, dendrograms were produced by the UPGMA (unweighted pair-group method of mathematical averages method). Out of the 71 primer combinations tested, 50 combinations revealed polymorphisms among the banana pepper lines, and a total of 123 polymorphic bands were obtained. In the bell pepper lines, 24 SRAP primer combinations were tested and 15 combinations had 25 polymorphic bands. Based on the UPGMA cluster analysis, the pepper lines divided into groups as bell peppers and banana peppers. While the genetic similarity among the banana pepper lines varied between 0.62 and 0.98, the genetic similarity among the bell pepper lines varied between 0.54 and 1.00. As a result, it can be stated that the SRAP markers can be used successfully for determining the genetic distances of the pepper inbred lines thus will help the breeding programme.

Keywords

• Pepper
• genetic relationship
• SRAP
• polymorphism

Kaynakça

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