Türkiye'nin Isparta ilinden elde edilen apple stem pitting virus izolatlarının yaygınlığı ve moleküler karakterizasyonu

Öz

Türkiye, sahip olduğu iklim çeşitliliği ve coğrafi özellikler nedeniyle meyvecilik açısından dünyada oldukça önemli bir ülke konumundadır. Bu meyvelerden bir tanesi olan elma ülkemizde yoğun olarak yetiştiriciliği yapılan bir meyve olup özellikle Akdeniz Bölgesi elma tarımı ile ön plana çıkmaktadır. Günümüzde viral hastalıklar elma tarımı içerisinde önemli bir verim kayıp unsuru olarak ele alınmaktadır. Bu çalışmada Türkiye’nin önemli bir elma üreticisi konumunda olan Isparta ilinde apple stem pitting virus (ASPV)’nin yaygınlık durumu belirlenmişve moleküler karakterizasyonu gerçekleştirilmiştir. Toplanan 70 adet elma yaprağı örneğinden 7’sinde ASPV enfeksiyonu DAS-ELISA ve RT-PCR yöntemi ile belirlenmiştir. Virüsün kılıf protein bölgesinin kısmi dizi bilgisi elde edilerek GenBank erişim numaraları alınmıştır. Oluşturulan benzerlik matrisinde izolatların GenBank referans izolatları ile 79-93% oranında nükleotid düzeyinde benzerlik gösterdiği tespit edilmiştir. GenBank’ta yer alan ve ASPV’nin filogenetik ayrımında güvenilir olduğu değerlendirilen belirli sayıda izolat seçilerek elde edilen filogenetik ağaç 6 gruba ayrılmış ve bu çalışma kapsamında elde edilen izolatlar filogrup 1 içerinde yer almıştır. Bu çalışma ASPV’nin Isparta bölgesinde yaygınlık durumunun incelendiği ilk çalışma özelliğindedir. Araştırma sonuçlarının ASPV’nin Türkiye’deki durumunun daha iyi anlaşılmasına katkı sunacağı düşünülmektedir.

Anahtar Kelimeler

• ASPV
• filogenetik ilişki
• moleküler karakterizasyon

Incidence and coat protein characterization of apple stem pitting virus isolates from Isparta province of Turkey

Abstract

Turkey is one of the major countries in the world in terms of fruit growing due to its climate diversity and geographical features. Apple, which is one of these fruits, is grown intensively in Turkey, and especially the Mediterranean Region is important for apple agriculture. Today, viral diseases are considered as an important yield loss factor in apple farming. This study investigated the incidence and molecular characterization of apple stem pitting virus (ASPV) in Isparta province, a significant apple-producing region in Turkey. By using the DAS-ELISA and RT-PCR methods, ASPV infection was found in 7 out of 70 collected apple leaf samples. The partial nucleotid sequences of ASPV were obtained and registered in GenBank for accession numbers. The generated similarity matrix by using the representative isolates revealed that the new ASPV isolates shared 79–93% of their nucleotide sequences with GenBank reference acessions. The isolates collected in this research were clustered in group 1 of the phylogenetic tree that was created by selecting a specific number of isolates from GenBank and thought to be reliable in the phylogenetic differentiation of ASPV. This is the first study to examine the prevalence of ASPV in the Isparta region and its phylogeny. It is possible that the results of the research will contribute to a better understanding of the situation of ASPV in Turkey.

Keywords

• ASPV
• phylogenetic relationship
• molecular characterization

Kaynakça

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