The Responses of Pollen Tubes to Spermidine Treatments in Actinidia deliciosa

Öz

In this study, the responses of pollen tubes to spermidine treatments (10 µM, 25 µM, 50 µM, 100 µM, 250 µM, or 500 µM) were investigated in Actinidia deliciosa, by focusing on pollen germination rate, pollen tube length, organizations of actin filaments, concentrations of Ca+2, pH, reactive oxygen species and distributions of callose and cellulose. According to findings, the only positive effect was detected after 10 µM spermidine treatment while the most negative acute effect was detected after 500 µM spermidine treatment and, further experiments were done in these groups. 10 µM spermidine increased the pollen tube length by changing the concentration of apex localized reactive oxygen species. 500 µM spermidine decreased the pollen tube length by changing the apex localized Ca+2, pH, and reactive oxygen species concentration. Findings would contribute to the understanding of the effects of polyamines on pollen tubes.

Anahtar Kelimeler

• Actin cytoskeleton
• cell wall
• pollen tube
• polyamine
• spermidine

Actinidia deliciosa’da Polen Tüplerinin Spermidin Uygulamalarına Verdiği Yanıtlar

Öz

Bu çalışmada Actinidia deliciosa'da polen tüplerinin spermidin uygulamalarına (10 µM, 25 µM, 50 µM, 100 µM, 250 µM veya 500 µM) verdiği yanıtlar, polen çimlenme oranı, polen tüp uzunluğu, aktin filament organizasyonu, Ca+2, pH, reaktif oksijen türlerinin konsantrasyonu ve kalloz ve selüloz dağılımına odaklanarak incelenmiştir. Bulgulara göre tek olumlu etki 10 µM spermidine uygulamasından sonra tespit edilirken, en olumsuz akut etki 500 µM spermidine uygulamasından sonra tespit edilmiş ve ileri deneyler bu gruplarda yapılmıştır. 10 µM spermidin, apekste lokalize olan reaktif oksijen türlerinin konsantrasyonunu değiştirerek polen tüpü uzunluğunu arttırmıştır. 500 µM spermidin ise apekste lokalize olan Ca+2, pH ve reaktif oksijen türlerinin konsantrasyonunu değiştirerek polen tüpü uzunluğunu azaltmıştır. Bulguların poliaminlerin polen tüpleri üzerindeki etkilerinin anlaşılmasına katkıda bulunabileceği düşünülmektedir.

Anahtar Kelimeler

• aktin hücre iskeleti
• hücre çeperi
• polen tüpü
• poliamin
• spermidin

Kaynakça

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