The Impact of Mycorrhiza and Trichoderma Treatment on Malondialdehyde Levels and Antioxidant Activity in Common Beans under Drought Stress

Yıl 2024, , 440 - 454, 24.12.2024

Hilal Yılmaz

https://doi.org/10.24180/ijaws.1535110

Öz

As global temperatures rise and drought conditions become increasingly frequent, the need to develop sustainable agricultural practices has become paramount. Enhancing crop resilience to water scarcity is essential to secure food supplies for a growing global population. This study examined the effects of Arbuscular Mycorrhizal Fungi (AMF) and Trichoderma harzianum on the physiological responses and growth of common bean (Phaseolus vulgaris) under 100% and 50% irrigation regimes. Under a 50% irrigation regime, AMF and Trichoderma harzianum inoculation led to substantial increases in plant height (34.5%) and root length (16.79%), compared to the control. Additionally, significant enhancements were observed in chlorophyll a (175%), chlorophyll b (194%), and total chlorophyll (180%) content in plants subjected to T. harzianum inoculation under water deficit. The application of AMF resulted in an 18% increase in total carotenoid content, showing its efficacy in sustaining photosynthetic pigments. Furthermore, the study revealed that both treatments significantly reduced malondialdehyde (MDA) accumulation, with reductions of 46.3% compared to the control under drought conditions. Catalase (CAT), increased by 201% with T. harzianum application under full irrigation and by 217% with AMF under reduced irrigation, highlighting the role of these biostimulants in mitigating oxidative stress. Principal component analysis (PCA) further confirmed that these treatments effectively maintained cellular integrity and enhanced stress tolerance. These findings underscore the potential of AMF and T. harzianum as vital tools in enhancing crop resilience against drought, with significant implications for sustainable agriculture in arid and semi-arid regions.

Anahtar Kelimeler

Drought tolerance, biostimulants, oxidative stress mitigation, AMF, global warming

Kuraklık Stresi Altındaki Fasulyelerde Mikoriza ve Trichoderma Tedavisinin Malondialdehit Düzeyleri ve Antioksidan Aktivitesi Üzerindeki Etkisi

Öz

Küresel sıcaklıkların artması ve kuraklık koşullarının giderek daha sık hale gelmesiyle birlikte, sürdürülebilir tarım uygulamalarını geliştirme ihtiyacı her zamankinden daha önemLi hale gelmiştir. Su kıtlığına karşı bitki direncinin artırılması, artan dünya nüfusunun gıda tedarikini güvence altına almak için hayati bir öneme sahiptir. Bu çalışmada, Arbusküler Mikoriza Fungus (AMF) ve Trichoderma harzianum'un, fasulye (Phaseolus vulgaris) bitkisinin fizyolojik tepkileri ve büyümesi üzerindeki etkileri %100 ve %50 sulama rejimLeri altında incelenmiştir. %50 sulama rejimi altında, AMF ve T. harzianum inokülasyonu, kontrol grubuna kıyasla bitki boyunda %34.5, kök uzunluğunda ise %16.79 oranında önemLi artışlar sağlamıştır. Ayrıca, su kısıtı koşullarında T. harzianum uygulanan bitkilerde klorofil a (%175), klorofil b (%194) ve toplam klorofil (%180) içeriğinde belirgin artışlar gözlemLenmiştir. AMF uygulaması, toplam karotenoid içeriğinde %18'lik bir artış sağlayarak fotosentetik pigmentlerin sürdürülebilirliğini göstermiştir. Bunun yanı sıra, her iki uygulamanın da malondialdehit (MDA) birikimini önemLi ölçüde azalttığı, kuraklık koşullarında kontrol grubuna kıyasla %46.3 oranında azalma sağladığı tespit edilmiştir. Katalaz (CAT), tam sulama altında T. harzianum uygulamasıyla %201, azaltılmış sulama altında ise AMF ile %217 artış göstermiştir, bu da bu biyostimülanların oksidatif stresi hafifletmedeki rolünü vurgulamaktadır. Temel bileşen analizi (PCA), bu tedavilerin hücresel bütünlüğü etkili bir şekilde koruduğunu ve stres toleransını artırdığını doğrulamıştır. Bu bulgular, AMF ve T. harzianum nın, kuraklığa karşı bitki direncini artırmada hayati araçlar olarak potansiyelini, kurak ve yarı kurak bölgelerde sürdürülebilir tarım için önemLi sonuçlarla birlikte ortaya koymaktadır.

Anahtar Kelimeler

Kuraklığa tolerans, biyostimülanlar, oksidatif stres azaltma, AMF, küresel ısınma

Kaynakça

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