Determination of Resistance Sources to BCMV and BCMNV in Some Common Bean (Phaseolus vulgaris L.) Cultivars Grown in Turkey

Abstract

Bean common mosaic virus (BCMV) and Bean common mosaic necrosis virus (BCMNV) are the common plant pathogens that cause significant yield losses in bean growing areas worldwide. The most effective and practical control method is to use of resistant common bean cultivars to BCMV and BCMNV. Many genes and molecular markers associated with resistance to these pathogens have been identified and used widely in breeding studies in the world. This study evaluated the presence of resistance sources in 39 common bean cultivars, widely grown in Turkey to BCMV and BCMNV with different molecular markers (SW-13, SBD-5, ROC11, eIFE4). Eleven common bean cultivars contained only one resistance gene. Also, the cultivars were evaluated for resistance gene combinations to these pathogens; twenty-five cultivars possessed gene combination of I+bc-12. Resistance in Fransız, Karabacak and 40 Günlük cultivars was controlled by the bc-12 and bc-3 gene combination. These resistance genes identified in this study could be used as valuable resistance sources for developing breeding materials resistant to BCMV and BCMNV.

Keywords

• BCMV
• BCMNV
• common bean
• molecular marker
• resistance

Ülkemizde Yetiştirilen Bazı Fasulye (Phaseolus vulgaris L.) Çeşitlerinin BCMV ve BCMNV’e Karşı Dayanıklılık Kaynaklarının Belirlenmesi

Öz

Bean common mosaic virus (BCMV) ve Bean common mosaic necrosis virus (BCMNV) dünyada fasulye üretim alanlarında yaygın olarak görülen ve önemli verim kayıplarına neden olan hastalık etmenleridir. Bu hastalık etmenlerine karşı en etkili ve pratik mücadele yöntemi ise dayanıklı çeşit kullanılmasıdır. Bu kapsamda dünyada bu etmenlere karşı dayanıklılıkla ilişkili çok sayıda gen ve bunlarla ilişkili moleküler markör tespit edilmiş olup ıslah çalışmalarında yaygın olarak kullanılmaktadır. Bu çalışmada ise ülkemizde yaygın olarak yetiştirilen 39 adet fasulye çeşidinin BCMV ve BCMNV hastalıklarına karşı dayanıklılık kaynakları farklı moleküler markörler (SW-13, SBD-5, ROC11, eIFE4) kullanılarak araştırılmıştır. Elde edilen sonuçlar test edilen fasulye genotiplerinden 11 adedinin tek bir dayanıklılık geni içerdiğini göstermiştir. Ayrıca farklı dayanıklılık gen kombinasyonları bakımından 25 fasulye çeşidinde I+bc-12 genlerinin bulunduğu görülmüştür. Fransız, Karabacak ve 40 Günlük fasulye çeşitlerinde ise dayanıklılığın bc-12 ve bc-3 genleri ile kontrol edildiği tespit edilmiştir. Yapılan çalışma ile tespit edilen dayanıklılık genlerinin söz konusu hastalıklara karşı dayanıklı ıslah materyallerinin geliştirilmesinde önemli birer dayanıklılık kaynağı olarak kullanılabilecekleri düşünülmektedir.

Anahtar Kelimeler

• BCMV
• BCMNV
• dayanıklılık
• fasulye
• moleküler markör

Kaynakça

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