‘Eşme’ Ayva Çeşidinin Farklı Meyve Kısımlarının Biyoaktif İçerikleri

Öz

Araştırma, ‘Eşme’ ayva çeşidinin farklı meyve kısımlarının pH, suda çözünebilir kuru madde, titre edilebilir asitlik, C vitamini, toplam fenolik, toplam flavonoid ve antioksidan aktivitesini (DPPH ve FRAP testlerine göre) belirlemek amacıyla yürütülmüştür. ‘Eşme’ ayva çeşidinin farklı meyve kısımlarında pH 3.57 (meyve eti)-4.03 (kabuk), suda çözünebilir kuru madde %5.40 (kabuk)-12.22 (meyve eti), titre edilebilir asitlik %0.21 (kabuk)-0.78 (meyve eti), C vitamini 11.68 (tüm meyve)-202.27 (kabuk) mg 100 g-1, toplam fenolik 56.78 (meyve eti)-974.32 (kabuk) mg 100 g-1, toplam flavonoid 39.89 (meyve eti)-291.10 (kabuk) mg 100 g-1, antioksidan aktivitesi 0.06 (meyve eti)-0.82 (kabuk) mmol 100 g-1 (DPPH testine göre) ve 0.30 (meyve eti)-2.74 (kabuk) mmol 100 g-1 (FRAP testine göre) arasında belirlenmiştir. Farklı meyve kısımlarına göre, toplam fenolik ile antioksidan aktivitesi (DPPH ve FRAP testlerine göre sırasıyla R2=1.0 ve R2=1.0) ve toplam flavonoid ile antioksidan aktivitesi (DPPH ve FRAP testlerine göre sırasıyla R2=1.0 ve R2=1.0) arasında yüksek pozitif bir ilişki tespit edilmiştir. Sonuç olarak, ‘Eşme’ ayva çeşidinin meyve kabuğunda meyve eti ve tüm meyveye göre toplam fenolik, toplam flavonoid ve antioksidan aktivitesinin daha yüksek olduğu belirlenmiştir.

Anahtar Kelimeler

• ayva
• kabuk
• meyve eti
• fenolik
• antioksidan

Bioactive Contents of Different Fruit Parts of ‘Eşme’ Quince Cultivar

Abstract

This research was carried out to determine pH, soluble solids content, titratable acidity, vitamin C, total phenolic, total flavonoid and antioxidant activity (according to DPPH and FRAP assays) of different fruit parts of ‘Eşme’ quince cultivar. pH, soluble solids content, titratable acidity, vitamin C, total phenolic, total flavonoid and antioxidant activity in the different fruit parts of ‘Eşme’ quince cultivar was determined from 3.57 (pulp) to 4.03 (peel), 5.40% (peel) to 12.22% (pulp), 0.21% (peel) to 0.78% (pulp), 11.68 (flesh) mg 100 g-1 to 202.27 (peel) mg 100 g-1, 56.78 (pulp) mg 100 g-1 to 974.32 (peel) mg 100 g-1, 39.89 (pulp) mg 100 g-1 to 291.10 (peel) mg 100 g-1, 0.06 mmol 100 g-1 (pulp) to 0.82 (peel) mmol 100 g-1 and 0.30 (pulp) mmol 100 g-1 to 2.74 (peel) mmol 100 g-1, respectively. According to different fruit parts, a high positive relation was determined from total phenolic to antioxidant activity (according to DPPH and FRAP assays R2=1.0 and R2=1.0, respectively) and total flavonoid to antioxidant activity (according to DPPH and FRAP assays R2=1.0 and R2=1.0, respectively). Consequently, in the peel of ‘Eşme’ quince cultivar was determined higher total phenolic, total flavonoid and, antioxidant activity than according to pulp and flesh.

Keywords

• quince
• peel
• pulp
• phenolic
• antioxidant

Kaynakça

1. Ajila, C. M., Naidu, K. A., Bhat, S. G., & Rao, U. P. (2007). Bioactive compounds and antioxidant potential of mango peel extract. Food Chemistry, 105(3), 982-988.
2. Alesiani, D., Canini, A., D’Abrosca, B., DellaGreca, M., Fiorentino, A., Mastellone, C., Monaco, P., & Pacifico, S. (2010).
3. Antioxidant and antiproliferative activities of phytochemicals from quince (Cydonia vulgaris) peels. Food Chemistry, 118(2), 199-207.
4. Ali, N., Abbas, A., Ali, A., Shahnawaz, M., Hussain, A., & Hussain, A. (2015). Physio-Chemical Nutritional and Sensory Evaluation of Local Quince Fruit of Nomal Village, Gilgit-Baltistan, Pakistan. Sciences, 4(6), 600-608.
5. Benzie, I. F., & Strain, J. J. (1996). The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Analytical Biochemistry, 239(1), 70-76.
6. Beyhan, Ö., Elmastaş, M., & Gedikli, F. (2010). Total phenolic compounds and antioxidant capacity of leaf, dry fruit and fresh fruit of feijoa (Acca sellowiana, Myrtaceae). Journal of Medicinal Plant Research, 4, 1065-1072.
7. Blanda, G., Rodrıguez-Roque, M. J., Comandini, P., Flores-Cordova, M. A., Salas-Salazar, N. A., Oscar, C. A., & Soto-Caballero, M. C. (2020). Phenolic profile and physicochemical characterization of quince (Cydonia oblonga Mill) fruits at different maturity index. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 48(4), 2306-2315.
8. Blois, M. S. (1958). Antioxidant determinations by the use of a stable free radical. Nature, 181(4617), 1199-1200.

9. Bolat, İ., & İkinci, A. (2015). Eşme ayva (Cydonia oblonga Miller) çeşidinin GAP Bölgesindeki performansı. Harran Tarım ve Gıda Bilimleri Dergisi, 19(1), 16-23.
10. Carvalho, M., Silva, B. M., Silva, R., Valentao, P., Andrade, P. B., & Bastos, M. L. (2010). First report on Cydonia oblonga Miller anticancer potential: differential antiproliferative effect against human kidney and colon cancer cells. Journal of Agricultural and Food Chemistry, 58(6), 3366-3370.
11. Çalhan, Ö., & Koyuncu, M. A. (2018). Eşme Ayva (Cydonia oblonga Mill.) Çeşidinde Optimum Derim Tarihini Belirlemek İçin Uygun Kriterlerin Seçimi. Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi, 28(2), 215-225.
12. Essafi-Benkhadir, K., Refai, A., Riahi, I., Fattouch, S., Karoui, H., & Essafi, M. (2012). Quince (Cydonia oblonga Miller) peel polyphenols modulate LPS-induced inflammation in human THP-1-derived macrophages through NF-κB, p38MAPK and Akt inhibition. Biochemical and Biophysical Research Communications, 418(1), 180-185.
13. FAO. (2021). Food and agriculture organization. https://www.fao.org/faostat/en/#data/QCL. Erişim tarihi: 26 Ekim 2021.
14. Fattouch, S., Caboni, P., Coroneo, V., Tuberoso, C. I., Angioni, A., Dessi, S., Marzouki, N., & Cabras, P. (2007).
15. Antimicrobial activity of Tunisian quince (Cydonia oblonga Miller) pulp and peel polyphenolic extracts. Journal of Agricultural and Food Chemistry, 55(3), 963-969.
16. Francini, A., & Sebastiani, L. (2013). Phenolic compounds in apple (Malus x domestica Borkh.): compounds characterization and stability during postharvest and after processing. Antioxidants, 2(3), 181-193.
17. Gerçekcioğlu, R., Gencer, S., & Atasever, Ö. Ö. (2014). Tokat ekolojisinde yetiştirilen “Eşme” ve “Limon” ayva (Cydonia vulgaris L.) çeşitlerinin bitkisel ve pomolojik özellikleri. International Journal of Agricultural and Natural Sciences, 7(1), 01-05.
18. Grinan, I., Galindo, A., Rodriguez, P., Morales, D., Corell, M., Centeno, A., Collado-Gonzalez, J., Torrecilas, A., Carbonell-Barrachina, A.A., & Hernández, F. (2019). Volatile composition and sensory and quality attributes of quince (Cydonia oblonga Mill.) fruits as affected by water stress. Scientia Horticulturae, 244, 68-74.
19. Hamauzu, Y., Inno, T., Kume, C., Irie, M., & Hiramatsu, K. (2006). Antioxidant and antiulcerative properties of phenolics from Chinese quince, quince, and apple fruits. Journal of Agricultural and Food Chemistry, 54(3), 765-772.
20. Hegedus, A., Papp, N., & Stefanovits-Bányai, É. (2013). review of nutritional value and putative health-effects of quince (Cydonia oblonga Mill.) fruit. International Journal of Horticultural Science, 19(3-4), 29-32.
21. Jhee, J. H., Kee, Y. K., Park, J. T., Chang, T. I., Kang, E. W., Yoo, T. H., Kang, S. W., & Han, S. H. (2019). A diet rich in vegetables and fruit and incident CKD: a community-based prospective cohort study. American Journal of Kidney Diseases, 74(4), 491-500.
22. Karadeniz, F., Burdurlu, H. S., Koca, N., & Soyer, Y. (2005). Antioxidant activity of selected fruits and vegetables grown in Turkey. Turkish Journal of Agriculture and Forestry, 29(4), 297-303.
23. Kolniak-Ostek, J. (2016). Chemical composition and antioxidant capacity of different anatomical parts of pear (Pyrus communis L.). Food Chemistry, 203, 491-497.
24. Legua, P., Serrano, M., Melgarejo, P., Valero, D., Martínez, J. J., Martínez, R., & Hernández, F. (2013). Quality parameters, biocompounds and antioxidant activity in fruits of nine quince (Cydonia oblonga Miller) accessions. Scientia Horticulturae, 154, 61-65.
25. Liu, H., Cao, J., & Jiang, W. (2015). Evaluation and comparison of vitamin C, phenolic compounds, antioxidant properties and metal chelating activity of pulp and peel from selected peach cultivars. LWT-Food Science and Technology, 63(2), 1042-1048.
26. Liu, R. H. (2003). Health benefits of fruit and vegetables are from additive and synergistic combinations of phytochemicals. The American Journal of Clinical Nutrition, 78(3), 517-520.
27. Manzoor, M., Anwar, F., Mahmood, Z., Rashid, U., & Ashraf, M. (2012). Variation in minerals, phenolics and antioxidant activity of peel and pulp of different varieties of peach (Prunus persica L.) fruit from Pakistan. Molecules, 17(6), 6491-6506.
28. Middleton E. J. R., & Kandaswami, C. (1986). The ımpact of plant flavonoids on mammalian biology: ımplications for ımmunity, ınflammation and cancer. In J.B. Harborne (Ed.), The Flavonoids (pp. 619-651). Chapman and Hall, London, England.
29. Öztürk, A., Demirsoy, L., Demirsoy, H., Asan, A., & Gül, O. (2015). Phenolic compounds and chemical characteristics of pears (Pyrus communis L.). International Journal of Food Properties, 18(3), 536-546.
30. Papp, N., Szabó, T., Szabó, Z., Nyéki, J., & Hegedűs, A. (2013). Antioxidant capacity and total polyphenolic content in quince (Cydonia oblonga Mill.) fruit. International Journal of Horticultural Science, 19(3-4), 33-35.
31. Rasheed, M., Hussain, I., Rafiq, S., Hayat, I., Qayyum, A., Ishaq, S., & Awan, M. S. (2018). Chemical composition and antioxidant activity of quince fruit pulp collected from different locations. International Journal of Food Properties, 21(1), 2320-2327.
32. Ricceri, F., Giraudo, M. T., Fasanelli, F., Milanese, D., Sciannameo, V., Fiorini, L., & Sacerdote, C. (2017). Diet and endometrial cancer: a focus on the role of fruit and vegetable intake, Mediterranean diet and dietary inflammatory index in the endometrial cancer risk. BMC Cancer, 17(1), 757.
33. Rop, O., Balik, J., Řezníček, V., Juríková, T., Škardová, P., Salaš, P., Sochor, J., Mlcek, J., & Kramářová, D. (2011). Chemical characteristics of fruits of some selected quince (Cydonia oblonga Mill.) cultivars. Czech Journal of Food Sciences, 29(1), 65-73.
34. Shaida, B., Singh, N. B., & Singh, K. (2020). In-vitro evaluation of anti-inflammatory and anti-microbial properties of ethanolic extract of Cydonia Oblonga seeds. Journal of Scientific & Industrial Research, 79, 49-52.
35. Silva, B. M., Andrade, P. B., Ferreres, F., Domingues, A. L., Seabra, R. M., & Ferreira, M. A. (2002). Phenolic profile of quince fruit (Cydonia oblonga Miller) (pulp and peel). Journal of Agricultural and Food Chemistry, 50(16), 4615-4618.
36. Silva, B. M., Andrade, P. B., Gonçalves, A. C., Seabra, R. M., Oliveira, M. B., & Ferreira, M. A. (2004). Influence of jam processing upon the contents of phenolics, organic acids and free amino acids in quince fruit (Cydonia oblonga Miller). European Food Research and Technology, 218(4), 385-389.
37. Silva, B. M., Andrade, P. B., Martins, R. C., Valentão, P., Ferreres, F., Seabra, R. M., & Ferreira, M. A. (2005). Quince (Cydonia oblonga Miller) fruit characterization using principal component analysis. Journal of Agricultural and Food Chemistry, 53(1), 111-122.
38. Silva, B. M., Seabra, R. M., Andrade, P. B., & Papadopoulos, K. N. (2008). Quince (Cydonia oblonga Miller): an interesting dietary source of bioactive compounds. Editor: Konstantinos N. Papadopoulos. Food Chemistry Research Developments, 243-266.
39. Stojanović, B. T., Mitić, S. S., Stojanović, G. S., Mitić, M. N., Kostić, D. A., Paunović, D. Ɖ., Arsic, B. B., & Pavlović, A. N. (2017). Phenolic profiles and metal ions analyses of pulp and peel of fruits and seeds of quince (Cydonia oblonga Mill.). Food Chemistry, 232, 466-475.
40. Szychowski, P. J., Munera-Picazo, S., Szumny, A., Carbonell-Barrachina, Á. A., & Hernández, F. (2014). Quality parameters, bio-compounds, antioxidant activity and sensory attributes of Spanish quinces (Cydonia oblonga Miller). Scientia Horticulturae, 165, 163-170.
41. Thomas, M., Crépeau, M. J., Rumpunen, K., & Thibault, J. F. (2000). Dietary fibre and cell-wall polysaccharides in the fruits of Japanese quince (Chaenomeles japonica). LWT-Food Science and Technology, 33(2), 124-131.
42. Tuna-Gunes, N., & Dumanoglu, H. (2005). Some fruit attributes of quince (Cydonia oblonga) based on genotypes during the pre‐harvest period. New Zealand Journal of Crop and Horticultural Science, 33(3), 211-217.
43. Valko, M., Leibfritz, D., Moncol, J., Cronin, M. T., Mazur, M., & Telser, J. (2007). Free radicals and antioxidants in normal physiological functions and human disease. The İnternational Journal of Biochemistry & Cell Biology, 39(1), 44-84.
44. Vieira, F. G. K., Borges, G. D. S. C., Copetti, C., Amboni, R. D. D. M. C., Denardi, F., & Fett, R. (2009). Physico-chemical and antioxidant properties of six apple cultivars (Malus domestica Borkh) grown in southern Brazil. Scientia Horticulturae, 122(3), 421-425.
45. Wojdylo, A., Oszmianski, J., & Bielicki, P. (2013). Polyphenolic composition, antioxidant activity, and polyphenol oxidase (PPO) activity of quince (Cydonia oblonga Miller) varieties. Journal of Agricultural and Food Chemistry, 61(11), 2762-2772.
46. Wootton-Beard, P. C., & Ryan, L. (2011). Improving public health?: The role of antioxidant-rich fruit and vegetable beverages. Food Research International, 44(10), 3135-3148.
47. Zhishen, J., Mengcheng, T., & Jianming, W. (1999). The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chemistry, 64(4), 555-559.