Assessment of Mineral Content Variations for Biofortification of the Bean Seed

Yıl 2018, Cilt: 4 Sayı: 2, 261 - 269, 30.12.2018

Mehmet Zahit Yeken , Hacer Akpolat Tolga Karaköy , Vahdettin Çiftçi

https://doi.org/10.24180/ijaws.455311

Cited By: 4

Öz

Germplasm collections are very
important for breeder to develop new cultivars with high mineral nutrients and
yield. Eighty-three Phaseolus landraces were collected from different provinces of Western Anatolia Region of Turkey
in 2015-2016. Twenty common bean lines were
selected according to morphological characterization results and weighted
scaling method in 2016. Phosphorus (P), potassium (K), copper (Cu), zinc
(Zn), manganese (Mn), iron (Fe), calcium (Ca), and magnesium (Mg) contents of these twenty common bean lines and two
commercial cultivars were tested under field conditions. Randomized block
design with three replicates was used for analysis in 2017 growing season on
the experimental farm of Bolu Abant Izzet Baysal University. The results showed
high level of variation among lines and cultivars in terms of P (0.94-1.30%), K
(2.38-3.59%), Cu (7.80-14.80 mg kg^-1),
Zn (19.74-66.68 mg kg^-1), Mn
(7.46-27.25 mg kg^-1), Fe
(48.98-182.45 mg kg^-1), Ca
(0.18-0.48 mg kg^-1) and Mg
(0.56-0.71 mg kg^-1) contents.
Positive correlations were found between K and Zn (r=0.447; P<0.05), P and Fe (r=0.485; P<0.05), Ca and Mg (r=0.693;
P<0.01). In principal component analysis
(PCA), the first 4 principal components accounted for approximately 73% of the
total variability. The lines, Ylv-14, Ylv-32, Blck-7, Blksr-3 and Brs-22 had
superior mineral contents for Fe and P, Cu and Mn, Ca and Mg, Zn, and K,
respectively. Therefore, these lines represent
promising candidates for biofortifying the bean seed and can be registered as
cultivars in Turkey. Moreover, these lines will be used further for identifying
the QTL regions by developing biparental mapping populations for an effective
breeding program in Turkey in near future.

Anahtar Kelimeler

Biofortification, micro- and macronutrient, Phaseolus vulgaris L.

Fasulye Tohumunun Biyofortifikasyonu için Mineral İçerik Vasyasyonlarının Değerlendirilmesi

Öz

Genetik kaynaklar ıslahçıların yüksek
verim ve mineral içeriğine sahip yeni çeşitler geliştirebilmesi için çok
önemlidir. 2015-2016 yılları arasında Türkiye’nin Batı Anadolu bölgesinin
farklı bölgelerinden seksen üç Phaseolus populasyonu
toplanmıştır. Morfolojik karakterizasyon sonuçları ve tartılı derecelendirmeye
göre yirmi fasulye hattı 2016 yılında seçilmiştir. Yirmi fasulye hattının ve
iki ticari çeşidin fosfor, potasyum, bakır, çinko, mangan, demir, kalsiyum ve
magnezyum içerikleri tarla koşullarında test edilmiştir. Deneme 2017 yılı
yetiştirme periyodunda Bolu Abant İzzet Baysal Üniversitesi, Araştırma ve
Uygulama Alanı’nda tesadüf blokları deneme desenine göre üç tekrarlamalı olarak
yürütülmüştür. Sonuçlar, fasulye hat ve çeşitleri arasında P (%0.94−1.30), K (%2.38-3.59), Cu (7.80-14.80 mg kg^-1), Zn
(19.74-66.68 mg kg^-1), Mn
(7.46-27.25 mg kg^-1), Fe
(48.98-182.45 mg kg^-1), Ca
(0.18-0.48 mg kg^-1) ve Mg
(0.56-0.71 mg kg^-1) yüksek
oranda varyasyon göstermiştir. K ve Zn
(r=0.447; P<0.05), P ve Fe (r=0.485;
P<0.05), Ca ve Mg (r=0.693; P<0.01) arasında pozitif yönde korelasyon
bulunmuştur. Ana bileşen analizine göre toplam çeşitliliğin %73’ünden ilk 4
temel komponentin sorumlu olduğu görülmüştür. Ylv-14, Ylv-32, Blck-7, Blksr-3
ve Brs-22 hatları sırasıyla Fe ve P, Cu ve Mn, Ca ve Mg, Zn, ve K bakımından
daha yüksek mineral içeriğine sahip olduğu tespit edilmiştir. Bu sebeplerden
dolayı, bu hatlar fasulye tohumlarının biyofortifikasyonu için ümitvar adayları
temsil etmekte ve Türkiye’de çeşit olarak tescillenebilir. Ayrıca bu hatlar,
yakın gelecekte etkili ıslah programları için biparental haritalama populasyonu
geliştirilerek QTL bölgelerinin tanımlanması amacıyla da kullanılabilir.

Anahtar Kelimeler

Biyofortifikasyon, mikro-makro mineral, Phaseolus vulgaris L.

Kaynakça

• Addinsoft 2016. XLSTAT 2016: Data Analysis and Statistics Software for Microsoft Excel. New York, USA.
• Baloch FS., Karaköy T., Demirbaş A., Toklu F., Özkan H and Hatipoğlu R., 2014. Variation of some seed mineral contents in open pollinated faba bean (Vicia faba L.) landraces from Turkey. Turkish Journal of Agriculture and Forestry, 38: 591-602.
• Beebe S., Gonzalez A and Rengifo J., 2000. Research on trace minerals in the common bean. Food and Nutrition Bulletin, 21: 387-391. Bitocchi E., Rau D., Bellucci E., Rodriguez M., Murgia ML., Gioia T., Santo D., Nanni L., Attene G and Papa R., 2017. Beans (Phaseolus ssp.) as a model for understanding crop evolution. Frontiers in Plant Science, 8: 722.
• Blair MW., Monserrate F., Beebe SE., Restrepo J and Ortubé J., 2010. Registration of high mineral common bean germplasm lines NUA35 and NUA56 from the red mottled seed class. Journal of Plant Growth Regulation, 4: 1-5.
• Blair MW., 2013. Mineral biofortification strategies for food staples: the example of common bean. Journal of Agricultural and Food Chemistry, 61: 8287-8294.
• Bremner JM., 1965. Method of Soil Analysis. Part 2. Chemical and Microbiological Methods, American Society of Agronomy Inc. USA. Broughton WJ., Hernandez G., Blair M., Beebe S., Gepts P and Vanderleyden J., 2003. Bean (Phaseolus spp.)-model food legumes. Plant Soil, 252: 55-128.
• Ceyhan E., Harmankaya M and Avcı MA., 2008. Effects of sowing dates and cultivars on protein and mineral contents of bean (Phaseolus vulgaris L.). Asian Journal of Chemistry, 20(7): 5601-5613.
• Çiftçi V., Şensoy S and Türkmen Ö., 2009. Van-Gevaş’ta Yaygın Olarak Yetiştirilen Yalancı Dermason Fasulye Populasyonunun Seleksiyon Yöntemiyle Islahı, Project report (TOVAG- 106O346).
• Çiftçi V., Şensoy S and Kulaz H., 2012. Doğu Anadolu’nun Güneyinde Yetiştirilen Fasulye Gen Kaynaklarının Toplanması Ve Değerlendirilmesi, Tübitak Project Report (109O163).
• Duc G., Bao S., Baum M., Redden B., Sadiki M., Suso MJ., Vishniakova M and Zong X., 2010. Diversity maintenance and use of Vicia faba L. genetic resources. Field Crops Research, 115: 270-278.
• Dutta SK., Chatterjee D., Sarkar D., Singh SB., Boopathi T., Kuotsu R., Vikramjeet K., Akoijam RS., Saha S., Vanlalhmangaiha., Malsawmzuali., Chowdhury S and Lungmuana., 2016. Common bean (Phaseolus vulgaris L., Fabaceae), landraces of Lushai hills in India: nutrients and antioxidants source for the farmers. Indian Journal of Traditional Knowledge, 15(2): 313-320.
• Elkoca E and Çınar T., 2015. The adaptation, agronomical and quality characteristcs of some dry bean (Phaseolus vulgaris L.) cultivars and lines under Erzurum ecological conditions. Anadolu Tarım Bilimleri Dergisi, 30(2): 141-153.
• Garg M., Sharma N., Sharma S., Kapoor P., Kumar A., Chunduri V and Arora P., 2018. Biofortified crops generated by breeding agronomy and transgenic approaches are improving lives of millions of people around the world. Frontiers in Nutrition, 5: 12.
• Graham RD., Welch RM and Bouis HE., 2001. Addressing micronutrient malnutrition through enhancing the nutritional quality of staple foods: principles, perspectives and knowledge gaps. Advances in Agronomy, 70: 77-142.
• Gelin JR., Forster S., Grafton SK., McClean PE and Rojas-Cifuentes GA., 2007. Analysis of seed zinc and other minerals in a recombinant inbred population of navy bean (Phaseolus vulgaris L.). Crop Science, 47: 1361-13.
• Gepts PTC., Osborn K., Rashka K and Bliss FA., 1986. Phaseolin protein variability in wild forms and landraces of the common bean (Phaseolus vulgaris): evidence for multiple centers of domestication. Economic Botany, 40: 451-468.
• Gesto-Seco EM., Moreda-Pineiro A., Bermejo-Barrera A and Barrera-Bermejo P., 2009. Multi-element determination in raft mussels by fast microwave-assisted acid leaching and inductively coupled plasma-optical emission spectrometry. Talanta, 72: 1178-1185.
• Islam FMA., Basford KE., Jara C., Redden RJ and Beebe SE., 2002. Seed compositional and disease resistance differences among gene pools in cultivated common bean. Genetic Resources and Crop Evolution, 49: 285-293.
• Kacar B and İnal A., 2008. Bitki Analizleri. Nobel Yayın Dağıtım, Ankara.
• Kantar F., Elkoca E., Eken C and Dönmez F., 2010. Kuzey Doğu Anadolu Bölgesi ve Çoruh Vadisi’nde Yetiştirilen Kuru Fasulye Gen Kaynaklarının Toplanması ve Değerlendirilmesi. Tubitak Project Report (107O400).
• Karaköy T., Erdem H., Baloch FS., Toklu F., Eker S., Kilian B and Özkan H., 2012. Diversity of macro-and micronutrients in the seeds of lentil landraces. The Scientific World Journal, 2012: 1-9.
• Karaköy T and Demirtaş A., 2017. Sivas ekolojik koşullarında yetiştirilen Türkiye orijinli yerel bezelye (Pisum sativum L.) genotiplerinin bazı besin elementi içerikleri bakımından değerlendirilmesi. Adnan Menderes Üniversitesi Ziraat Fakültesi Dergisi, 14(2): 7-11.
• Khan MA., Fuller MP and Baloch FS., 2008. Effect of soil applied zinc sulphate on wheat (Triticum aestivum L.) grown on a calcareous soil in Pakistan. Cereal Research Communications, 36: 571-582.
• Kwak M and Gepts P., 2009. Structure of genetic diversity in the two major gene pools of common bean (Phaseolus vulgaris L., Fabaceae). Theoretical and Applied Genetics, 118: 979-992.
• Madakbaş SY and Ergin M., 2011. Morphological and phenological characterization of Turkish bean (Phaseolus vulgaris L.) genotypes and their present variation states. African Journal of Agricultural Research, 6(28): 6155-6166.
• Moraghan JT and Grafton K., 2001. Genetic diversity and mineral composition of common bean seed. Journal of the Science of Food and Agriculture, 81: 404-408.
• Murphy J and Riley JP., 1962. A modified single solution for the determination of phosphate in natural waters. Analytica Chimica Acta, 27: 31-36.
• Nadeem MA., Habyarimana E., Çiftçi V., Nawaz MA., Karaköy T., Comertpay G., Shahid MQ., Hatipoğlu R., Yeken MZ., Ali F., Ercişli S., Chung G and Baloch FS., 2018. Characterization of genetic diversity in Turkish common bean gene pool using phenotypic and whole-genome DArTseq-generated silicoDArT marker information. PloS one, 13(10): e0205363.
• Petry N., Boy E., Wirth JP and Hurrell RF., 2015. Review: the potential of the common bean (Phaseolus vulgaris) as a vehicle for iron biofortification. Nutrients, 7(2): 1144-1173.
• Pinheiro C., Baeta JP., Pereira AM., Domingues H and Ricardo CP., 2010. Diversity of seed mineral composition of Phaseolus vulgaris L. germplasm. Journal of Food Composition and Analysis, 23(4): 319-325.
• Schmutz J., McClean PE., Mamidi S., Wu GA., Cannon SB., Grimwood J., Jenkins J., Shu S., Song Q., Chavarro C., Torres-Torres M., Geffroy V., Moghaddam S., Gao D., Abernathy B., Barry K., Blair M., Brick MA., Chovatia M., Gepts P., Goodstein DM., Gonzales M., Hellsten U., Hyten DL., Jia G., Kelly JD., Kudrna D., Lee R., Richard MM., Miklas PN., Osorno JM., Rodrigues J., Thareau V., Urrea CA., Wang M., Yu Y., Zhang M., Wing RA., Cregan PB., Rokhsar DS and Jackson SA., 2014. A reference genome for common bean and genome-wide analysis of dual domestications. Nature Genetics, 46: 707-13.
• Sönmez F., Gülser F., Karaca S and Gökkaya T., 2018. Determination of some physical and chemical properties of research area soils of Bolu Abant İzzet Baysal University. International Journal of Agricultural and Wildlife Sciences, 4(1): 68-78.
• Sperotto RA and Ricachenevsky FK., 2017. Common bean Fe biofortification using model species’ lessons. Frontiers in Plant Science, 8: 2187.
• Welch RM and Graham RD., 2004. Breeding for micronutrients in staple food crops from a human nutrition perspective. Journal of Experimental Botany, 55: 353-364.
• White PJ and Broadley MR., 2005. Biofortifying crops with essential mineral elements. Trends in Plant Science, 10: 586-593.
• WHO 2009. Global health risks, mortality and burden of disease attributable to selected major risks. Geneva, Switzerland, WHO.
• Yeken MZ., Kantar F., Çancı H., Özer G and Çiftçi V., 2018a. Breeding of dry bean cultivars using Phaseolus vulgaris landraces in Turkey. International Journal of Agricultural and Wildlife Sciences, 4(1): 45-54.
• Yeken MZ., Baloch FS., Nadeem MA., Çiftçi V and Karaköy T., 2018b. Variation of some seed mineral contents in 236 bean (Phaseolus vulgaris L.) landraces in Turkey. International Agriculture Congress, 3-6 May, Komrat, Moldova.
• Yücel C., Baloch FS and Ozkan H., 2009. Genetic analysis of some physical properties of bread wheat grain (Triticum aestivum L. em Thell). Turkish Journal of Agriculture and Forestry, 33: 525-535.