Araştırma Makalesi
BibTex RIS Kaynak Göster

Species Identification of Small Mammal Fauna in Bilecik Province and Molecular Researches for the Protection of Gene Resources

Yıl 2019, Cilt: 5 Sayı: 1, 149 - 160, 30.06.2019
https://doi.org/10.24180/ijaws.478168

Öz

In this study, it was aimed to
determine the ecological, karyological and morphological properties of the
fauna of small mammals from Bilecik province in between 2016-2017 years.
Animals were identified by analyzing their nest structure, chromosomal feature
and pelage color accordingly species-specific characteristics were recorded.
The chromosome number and structure are presented as ideograms. In total 9
species were recorded, in which Mus
musculus domesticus
, Rattus rattus,
Microtus lydius, Crocidura suaveolens species
were the first data in Bilecik province. For the construction of cDNA’s, RNA
isolation was performed from the blood samples of these identified animals.
These cDNA’s were stocked for subsequent molecular studies. According to the
field work the most sufficient habitats for small mammals were determined as
forest coast and cultivated lands in Bilecik province
.

Kaynakça

  • Albaba, I. (2016). The terrestrial mammals of Palestine: A preliminary checklist. International Journal of Fauna and Biological Studies, 4, 28-35.
  • Albayrak, İ., & Arslan, A. (2006). Contribution to the Taxonomical and Biological Characteristics of Sciurus anomalus in Turkey (Mammalia: Rodentia). Turkish Journal of Zoology, 30, 111-116.
  • Al-Taweel, K., Fernando, W. G .D., Brûlé-Babel, A. L. (2011). Construction and Characterization of a cDNA Library from Wheat Infected with Fusarium graminearum Fg 2. International Journal of Molecular Science, 12, 613-626.
  • Arslan, A., & Bölükbaş, F. (2010). C-heterochromatin and NORs distribution of mole rat, Nannospalax xanthodon from Aksaray, Turkey. Caryologia, 63, 398-404.
  • Arslan, A., & Zima, J. (2015). Heterochromatin distribution and localization of nucleolar organizing regions in the 2n=52 cytotypes of Nannospalax xanthodon and N. ehrenbergi from Turkey. Zoological Studies, 54,1-6.
  • Arslan, A., Kryštufek, B., Matur, F., & Zima, J. (2016). Review of chromosome races in blind mole rats (Spalax and Nannospalax). Folia Zoologica, 65, 249-301.
  • Aşan Baydemir, N. (2011). Chromosomal Polymorphism of Rattus rattus (Linnaeus, 1758) (Rodentia: Muridae) in Central Anatolia. Folia Biologica, 59, 1-2.
  • Berry, R. J., & Scriven, P. N. (2005). The house mouse: a model and motor for evolutionary understanding. Biological Journal of the Linnean Society, 84, 335-347.
  • Coşkun, Y., El Namee, A., & Kaya, A. (2012). Karyotype of Nannospalax ehrenbergi (Nehring, 1898) (Rodentia: Spalacidae) in the Mosul Province, Iraq. Hystrix, 23, 75-78.
  • Çolak, E., Yiğit, N., & Çolak, R. (2004). Taxonomic Status and Distribution of Apodemus mystacinus (Danford and Alston, 1877) (Mammalia: Rodentia) in Turkey. Turkish Journal of Zoology, 28, 285-294.
  • Çolak, E., Yiğit, N., Sözen, M., Çolak, R., Özkurt, Ş., Kankılıç, T., & Kankılıç, T. (2006). The Morphological analysis of Mus domesticus and Mus macedonicus (Mammalia: Rodentia) in Turkey. Turkish Journal of Zoology, 30, 309-317.
  • Gözcelioğlu, B., Çolak, R., Çolak, E., & Yiğit, N. (2005). A Study on Mus domesticus Rutty, 1772 and Mus macedonicus Petrov and Ruzíc, 1983 (Mammalia: Rodentia) Distributed along the Line of Ankara, Bolu and Zonguldak. Turkish Journal of Zoology, 29, 133-140.
  • Gözütok, S. (2017). Bursa İli Memeli (Classis: Mammalia) Faunası ve Türlerin Koruma Statüleri. Uluslararası Tarım ve Yaban Hayatı Bilimleri Dergisi, 3, 120-130.
  • Guo, Y., Liu, C., Lu, T., Liu, D., Bai, C., Li, X., Ma, Y., & Guan, W. (2014). Primary analysis of the expressed sequence tags from a full-length enrıched cDNA library of siberian tiger (panthera tigris altaica). The Journal of Animal & Plant Sciences, 25, 139-145.
  • Jojić, V., Bugarski-Stanojević, V., Blagojević, J., & Vujošević, M. (2014). Discrimination of the sibling species Apodemus flavicollis and A. sylvaticus (rodentia, muridae). A Journal of Comparative Zoology, 253, 261-269.
  • Kryštufek, B. (2006). Cranial variability in the Eastern hedgehog Erinaceus concolor (Mammalia: Insectivora). Journal of Zoology, 258, 365-373.
  • Kryštufek, B., & Vohralík, V. (2009). Mammals of Turkey and Cyprus (Rodentia II: Cricetinae, Muridae, Spalacidae, Calomyscidae, Capromyidae, Hystricidae, Castoridae). Koper, Slovenia: Univerza na Primorskem.
  • Kryštufek, B., Nedyalkov, N., Astrin, J. J., & Hutterer, Rainer. (2018). News from the Balkan refugium: Thrace has an endemic mole species (Mammalia: Talpidae). Bonn zoological Bulletin 67, 41-57.
  • Matur, F., & Sözen, M. (2005). A karyological study on subterrranean mole rats of the Spalax leucodon Nordmann, 1840 (Mammalia: Rodentia) superspecies in northwestern Turkey. Zoology in the Middle East, 36, 5-10.
  • Musser, G. G., & Carleton, M. D. (2005). Mammals Species of the World A Taxonomic and Geographic Reference. Johns Hopkins University Press.
  • Özen, A. S., & Yılmaz, A. A. (2012). Rattus norvegıcus (Berkenhout, 1769) 'un Laboratuvarda Beslenme Davranışı. Dumlupınar Üniversitesi, Fen Bilimleri Enstitüsü Dergisi, 1302-3055.
  • Selçuk, A. Y., & Kefelioğlu, H. (2017). Cytogenetic characteristic of the Caucasian pygmy shrew (Sorex volnuchini) and Levant mole (Talpa levantis) (Mammalia: Eulipotyphla) in northern Anatolia, Turkey. Turkish Journal of Zooloy, 41, 963-969.
  • Selçuk, A. Y., & Kefelioğlu, H. (2018). Cytogenetic Characteristics of Crocidura suaveolens, Crocidura leucodon, Sorex raddei (Mammalia: Eulipotyphla) from Turkey: Constitutive Heterochromatin Distribution. Türk Doğa ve Fen Dergisi, 7,15-20.
  • Sözen, M., Çolak, F., Sevindik, M., & Matur, F. (2013). Cytotypes of Nannospalax xanthodon (Satunin, 1898) (Rodentia, Spalacidae) from western Anatolia. Turkish Journal of Zooloy, 37, 462-469.
  • Orman ve Su İşleri Bakanlığı, (2012). Biyolojik Çeşitliliği İzleme ve Değerlendirme Raporu.
  • Topaktaş, M., & Rencüzoğulları, E. (2010). Sitogenetik. Seçkin Yayıncılık, Yayın No:2, İstanbul.
  • Yağcı, T. (2018). C- and NOR-banding karyotype analysis of Nannospalax xanthodon (2n=52, 2n=60) and new locality for 2n=52 cytotype from western Anatolia. Caryologia, 71, 7-12.
  • Yiğit, N., Çolak, E., Sözen, M., & Karataş, A. (2006). Rodents of Türkiye "Türkiye Kemiricileri". Meteksan AŞ, Ankara.
  • Yiğit, N., Markov, G., Çolak, E., Kocheva, M., Saygılı, F., Yüce, D., & Çam, P. (2012). Phenotypic Features of the ‘Guentheri’ Group Vole (Mammalia: Rodentia) in Turkey and Southeast Bulgaria: Evidence for Its Taxonomic Detachment. Acta Zoologıca Bulgarıca, 64, 23-32.
  • Yuan, Y., Long, P., Jiang, C., Li, M., & Huang, L. (2015). Development and characterization of simple sequence repeat (SSR) markers based on a full-length cDNA library of Scutellaria baicalensis. Genomics, 105, 61-7.

Bilecik İli Küçük Memeli Faunasının Tür Tespiti ve Gen Kaynaklarının Korunmasına Yönelik Moleküler Araştırmalar

Yıl 2019, Cilt: 5 Sayı: 1, 149 - 160, 30.06.2019
https://doi.org/10.24180/ijaws.478168

Öz

Bu araştırmada
2016-2017 yılları arasında Bilecik ili küçük memeli faunasının ekolojik,
karyolojik ve morfolojik olarak belirlenmesi amaçlanmıştır. Hayvanların
teşhisinde yuva yapısı, kürk rengi ve kromozomal özellikler incelenerek türe
özgü karakteristikler kaydedilmiştir. Kromozom sayı ve yapıları ideogramlar
halinde sunulmuştur. Araştırma sonucunda tespit edilen Mus musculus domesticus, Rattus
rattus
, Microtus lydius, Crocidura suaveolens Bilecik ili için ilk veriler olup toplam 9 tür kaydı
verilmiştir. Tür tespiti yapılan hayvanlara ait kan örneklerinden cDNA
oluşturulmak üzere RNA izolasyonu yapılmıştır. Elde edilen cDNA’lar daha
sonraki moleküler araştırmalarda kullanılmak üzere stoklanmıştır. Bilecik
ilinde yapılan arazi çalışmalarına göre küçük memeliler için en elverişli
habitatlar ekili araziler ve orman kıyısı alanlarıdır. 

Kaynakça

  • Albaba, I. (2016). The terrestrial mammals of Palestine: A preliminary checklist. International Journal of Fauna and Biological Studies, 4, 28-35.
  • Albayrak, İ., & Arslan, A. (2006). Contribution to the Taxonomical and Biological Characteristics of Sciurus anomalus in Turkey (Mammalia: Rodentia). Turkish Journal of Zoology, 30, 111-116.
  • Al-Taweel, K., Fernando, W. G .D., Brûlé-Babel, A. L. (2011). Construction and Characterization of a cDNA Library from Wheat Infected with Fusarium graminearum Fg 2. International Journal of Molecular Science, 12, 613-626.
  • Arslan, A., & Bölükbaş, F. (2010). C-heterochromatin and NORs distribution of mole rat, Nannospalax xanthodon from Aksaray, Turkey. Caryologia, 63, 398-404.
  • Arslan, A., & Zima, J. (2015). Heterochromatin distribution and localization of nucleolar organizing regions in the 2n=52 cytotypes of Nannospalax xanthodon and N. ehrenbergi from Turkey. Zoological Studies, 54,1-6.
  • Arslan, A., Kryštufek, B., Matur, F., & Zima, J. (2016). Review of chromosome races in blind mole rats (Spalax and Nannospalax). Folia Zoologica, 65, 249-301.
  • Aşan Baydemir, N. (2011). Chromosomal Polymorphism of Rattus rattus (Linnaeus, 1758) (Rodentia: Muridae) in Central Anatolia. Folia Biologica, 59, 1-2.
  • Berry, R. J., & Scriven, P. N. (2005). The house mouse: a model and motor for evolutionary understanding. Biological Journal of the Linnean Society, 84, 335-347.
  • Coşkun, Y., El Namee, A., & Kaya, A. (2012). Karyotype of Nannospalax ehrenbergi (Nehring, 1898) (Rodentia: Spalacidae) in the Mosul Province, Iraq. Hystrix, 23, 75-78.
  • Çolak, E., Yiğit, N., & Çolak, R. (2004). Taxonomic Status and Distribution of Apodemus mystacinus (Danford and Alston, 1877) (Mammalia: Rodentia) in Turkey. Turkish Journal of Zoology, 28, 285-294.
  • Çolak, E., Yiğit, N., Sözen, M., Çolak, R., Özkurt, Ş., Kankılıç, T., & Kankılıç, T. (2006). The Morphological analysis of Mus domesticus and Mus macedonicus (Mammalia: Rodentia) in Turkey. Turkish Journal of Zoology, 30, 309-317.
  • Gözcelioğlu, B., Çolak, R., Çolak, E., & Yiğit, N. (2005). A Study on Mus domesticus Rutty, 1772 and Mus macedonicus Petrov and Ruzíc, 1983 (Mammalia: Rodentia) Distributed along the Line of Ankara, Bolu and Zonguldak. Turkish Journal of Zoology, 29, 133-140.
  • Gözütok, S. (2017). Bursa İli Memeli (Classis: Mammalia) Faunası ve Türlerin Koruma Statüleri. Uluslararası Tarım ve Yaban Hayatı Bilimleri Dergisi, 3, 120-130.
  • Guo, Y., Liu, C., Lu, T., Liu, D., Bai, C., Li, X., Ma, Y., & Guan, W. (2014). Primary analysis of the expressed sequence tags from a full-length enrıched cDNA library of siberian tiger (panthera tigris altaica). The Journal of Animal & Plant Sciences, 25, 139-145.
  • Jojić, V., Bugarski-Stanojević, V., Blagojević, J., & Vujošević, M. (2014). Discrimination of the sibling species Apodemus flavicollis and A. sylvaticus (rodentia, muridae). A Journal of Comparative Zoology, 253, 261-269.
  • Kryštufek, B. (2006). Cranial variability in the Eastern hedgehog Erinaceus concolor (Mammalia: Insectivora). Journal of Zoology, 258, 365-373.
  • Kryštufek, B., & Vohralík, V. (2009). Mammals of Turkey and Cyprus (Rodentia II: Cricetinae, Muridae, Spalacidae, Calomyscidae, Capromyidae, Hystricidae, Castoridae). Koper, Slovenia: Univerza na Primorskem.
  • Kryštufek, B., Nedyalkov, N., Astrin, J. J., & Hutterer, Rainer. (2018). News from the Balkan refugium: Thrace has an endemic mole species (Mammalia: Talpidae). Bonn zoological Bulletin 67, 41-57.
  • Matur, F., & Sözen, M. (2005). A karyological study on subterrranean mole rats of the Spalax leucodon Nordmann, 1840 (Mammalia: Rodentia) superspecies in northwestern Turkey. Zoology in the Middle East, 36, 5-10.
  • Musser, G. G., & Carleton, M. D. (2005). Mammals Species of the World A Taxonomic and Geographic Reference. Johns Hopkins University Press.
  • Özen, A. S., & Yılmaz, A. A. (2012). Rattus norvegıcus (Berkenhout, 1769) 'un Laboratuvarda Beslenme Davranışı. Dumlupınar Üniversitesi, Fen Bilimleri Enstitüsü Dergisi, 1302-3055.
  • Selçuk, A. Y., & Kefelioğlu, H. (2017). Cytogenetic characteristic of the Caucasian pygmy shrew (Sorex volnuchini) and Levant mole (Talpa levantis) (Mammalia: Eulipotyphla) in northern Anatolia, Turkey. Turkish Journal of Zooloy, 41, 963-969.
  • Selçuk, A. Y., & Kefelioğlu, H. (2018). Cytogenetic Characteristics of Crocidura suaveolens, Crocidura leucodon, Sorex raddei (Mammalia: Eulipotyphla) from Turkey: Constitutive Heterochromatin Distribution. Türk Doğa ve Fen Dergisi, 7,15-20.
  • Sözen, M., Çolak, F., Sevindik, M., & Matur, F. (2013). Cytotypes of Nannospalax xanthodon (Satunin, 1898) (Rodentia, Spalacidae) from western Anatolia. Turkish Journal of Zooloy, 37, 462-469.
  • Orman ve Su İşleri Bakanlığı, (2012). Biyolojik Çeşitliliği İzleme ve Değerlendirme Raporu.
  • Topaktaş, M., & Rencüzoğulları, E. (2010). Sitogenetik. Seçkin Yayıncılık, Yayın No:2, İstanbul.
  • Yağcı, T. (2018). C- and NOR-banding karyotype analysis of Nannospalax xanthodon (2n=52, 2n=60) and new locality for 2n=52 cytotype from western Anatolia. Caryologia, 71, 7-12.
  • Yiğit, N., Çolak, E., Sözen, M., & Karataş, A. (2006). Rodents of Türkiye "Türkiye Kemiricileri". Meteksan AŞ, Ankara.
  • Yiğit, N., Markov, G., Çolak, E., Kocheva, M., Saygılı, F., Yüce, D., & Çam, P. (2012). Phenotypic Features of the ‘Guentheri’ Group Vole (Mammalia: Rodentia) in Turkey and Southeast Bulgaria: Evidence for Its Taxonomic Detachment. Acta Zoologıca Bulgarıca, 64, 23-32.
  • Yuan, Y., Long, P., Jiang, C., Li, M., & Huang, L. (2015). Development and characterization of simple sequence repeat (SSR) markers based on a full-length cDNA library of Scutellaria baicalensis. Genomics, 105, 61-7.
Toplam 30 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Bölüm Yaban Hayatı ve Ekolojisi
Yazarlar

Tuba Yağcı 0000-0003-1705-5107

Yayımlanma Tarihi 30 Haziran 2019
Gönderilme Tarihi 3 Kasım 2018
Kabul Tarihi 12 Aralık 2018
Yayımlandığı Sayı Yıl 2019 Cilt: 5 Sayı: 1

Kaynak Göster

APA Yağcı, T. (2019). Bilecik İli Küçük Memeli Faunasının Tür Tespiti ve Gen Kaynaklarının Korunmasına Yönelik Moleküler Araştırmalar. International Journal of Agricultural and Wildlife Sciences, 5(1), 149-160. https://doi.org/10.24180/ijaws.478168

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