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Effects of Azadirachtin and Spinosad on the Survival and Virulence of Some Local Entomopathogenic Nematodes Isolates

Yıl 2019, Cilt: 5 Sayı: 2, 280 - 285, 13.12.2019
https://doi.org/10.24180/ijaws.591287

Öz

Entomopathogenic nematodes (EPN) are often used in
combination with biopesticides in biologics control applications. Determination
of the toxic effects of biopesticides on EPNs is crucial for achieving the desired
results from these applications. The effects of commonly used biopesticides
[Nimiks (40 g L-1 Azadirachtin) and Laser (Spinosad 480 g L-1)]
on the survival and virulence of some local EPN species (Steinernema feltiae E-76, S.
littorale
MGZ-4-S, Heterorhabditis
indica
216-H) were investigated on the last larval instar of Galleria mellonella L. (Lepidoptera:
Pyralidae) larvae in 24 and 48 hours periods in laboratory conditions (25oC±2,
R.H.  %65±5). Of the EPN species directly
exposed to the highest recommended doses of Spinosad and Azadirachtin, the most
tolerant species was H. indica 216-H
with the survival rate of 94% during the first 24 hours. Steinernema littorale MGZ-4-S was the most susceptible species to
Azadirachtine with the mortality rate of 11% and 12% respectively in 24 and 48
hours periods. Mortality rates of the tested EPN species on the G. mellonella larvae ranged from 80% to
100%. The obtained results revealed that the EPN species tested are compatible
with Spinosad and Azadirachtin under laboratory conditions.

Kaynakça

  • Bayramoglu. Z., Demir, I., Inan, C., & Demirbag, Z. (2018). Efficacy of native entomopathogenic nematodes from Turkey against the alder leaf beetle, Agelastica alni L.(Coleoptera: Chrysomelidae) under laboratory conditions. Egyptian Journal of Biological Pest Control, 28(1), 17.
  • Bortoluzzi, L., Alves, L.F.A., Alves, V. S., & Holz, N. (2013). Entomopathogenic nematodes and their interaction with chemical insecticide aiming at the control of Banana weevil borer, Cosmopolites sordidus Germar (Coleoptera: Curculionidae). Arquivos do Instituto Biológico, 80(2), 183-192.
  • Canhilal, R., Waeyenberge, L., Toktay, H., Bozbuga, R., Çetintas, R., & Imren, M. (2016). Distribution of Steinernematids and Heterorhabditids (Rhabditida: Steinernematidae and Heterorhabditidae) in the Southern Anatolia Region of Turkey. Egyptian Journal of Biological Pest Control, 26(4).
  • Canhilal, R., Waeyenberge, L., Yüksel, E., Koca, A.S., Deniz, Y., & Imren, M. (2017). Assessment of the Natural Presence of Entomopathogenic Nematodes in Kayseri Soils. Turkey. Egyptian Journal of Biological Pest Control, 27(2).
  • Chavan, S.N., Somasekhar, N., & Katti, G. (2018). Compatibility of entomopathogenic nematode Heterorhabditis indica (Nematoda: Heterorhabditidae) with agrochemicals used in the rice ecosystem. Journal of Entomologu and Zoology Studies, 6(4), 527-532.
  • Karabörklü, S.. Ayvaz, A., Yilmaz, S., Azizoglu, U., & Akbulut, M. (2015). Native entomopathogenic nematodes isolated from Turkey and their effectiveness on pine processionary moth, Thaumetopoea wilkinsoni Tams. International journal of pest management, 61(1), 3-8.
  • Kaya, H.K. (1990). Soil Ecology. In: R. Gauglerr, and H.K. Kaya (Eds.), Entomopathogenic Nematodes in Biological Control (pp. 93-115). Florida, USA: CRC Press. Kaya, H., & Gaugler, R. (1993). Entomopathogenic nematodes. Annual Review of Entomology. CRC Press, USA.
  • Kim, K.H.. Kabir, E., & Jahan, S.A. (2017). Exposure to pesticides and the associated human health effects. Science of the Total Environment. 575, 525-535.
  • Krishnayyaand, P. V., & Grewal, P. S. (2002). Effect of neem and selected fungicides on viability and virulence of the entomopathogenic nematode Steinernema feltiae. Biocontrol Science and Technology, 12(2), 259-266.
  • Koppenhöfer, A. M., & Kaya, H. K. (1998). Synergism of imidacloprid and an entomopathogenic nematode: a novel approach to white grub (Coleoptera: Scarabaeidae) control in turfgrass. Journal of Economic Entomology, 91(3), 618-623.
  • Mahmoud, M. F., Mahfouz, H. M., & KM, M. (2016). Compatibility of entomopathogenic nematodes with neonicotinoids and Azadirachtin insecticides for controlling the black cutworm, Agrotis ipsilon (Hufnagel) in canola plants. International Journal of Research in Engineering and Science, 2(1), 11-18.
  • Morales-Rodriguez, A., & Peck, D. C. (2009). Synergies between biological and neonicotinoid insecticides for the curative control of the white grubs Amphimallon majale and Popillia japonica. Biological control, 51(1), 169-180.
  • Ozdemir, E., & Gozel, U. (2017). Efficiency of Some Plant Essential Oils on Root-Knot Nematode Meloidogyne incognita. Journal of Agricultural Science and Technology A, 7(3), 178-183.
  • Ozdemir, E., & Gozel, U. (2018). Nematicidal Activities of Essential Oils Against Meloidogyne Incognita On Tomato Plant. Fresenius Environmental Bulletin, 27(6), 4511-4517.
  • Parrón, T., Requena, M., Hernández, A. F., & Alarcón, R. (2014). Environmental exposure to pesticides and cancer risk in multiple human organ systems. Toxicology Letters, 230(2), 157-165.
  • Radova, S. (2011). Effect of selected pesticides on survival and virulence of two nematode species. Polish Journal of Environmental Studies, 20(1), 181-185.
  • Raheel, M., Javed, N., Khan, S. A., & Ahmed, S. (2017). Exploiting the biocontrol potential of entomopathogenic nematodes in combination with chemicals against greater wax moth (Galleria mellonella L.). Journal of Animal & Plant Sciences, 27(3).
  • Sabarwal, A., Kumar, K., & Singh, R. P. (2018). Hazardous effects of chemical pesticides on human health-cancer and other associated disorders. Environmental toxicology and pharmacology, 63,103-114.
  • Sabino, P.H.S, F.S. Sales, E.J. Guevara, A. Moino. Jr. and C.C. Filgueiras (2014). Compatibility of entomopathogenic nematodes (Nematoda; Rhabditida) with insecticides used in the tomato crop. Nematoda 1.
  • SPSS. (2013). IBM SPSS Statistics 21.0 for Windows. Armonk, NY.
  • Ulu, T. C., Sadic, B., & Susurluk, I. A. (2016). Effects of different pesticides on virulence and mortality of some entomopathogenic nematodes. Invertebrate Survival Journal, 13(1), 111-115.
  • Yüksel, E., Açıkgöz, Ç., Demirci, F., & Muștu, M. (2017). Effects of the entomopathogenic fungi, Beauveria bassiana, Isaria farinosa and Purpureocillium lilacinum, on eggs of Tuta absoluta (Meyrick)(Lepidoptera: Gelechiidae). Türkiye Biyolojik Mücadele Dergisi, 8(1), 39-47.
  • Yuksel, E., Taskesen, Y. E., Erarslan, D., & Canhilal, R. (2018). Effectiveness of different entomopathogenic nematode species against the variegated cutworm, Peridroma saucia (Hubner)(Lepidoptera: Noctuidae). Egyptian Journal of Biological Pest Control, 28(1), 8.
  • Yuksel, E., & Canhilal, R. (2018). Evaluation of local isolates of entomopathogenic nematodes for the management of black cutworm, Agrotis ipsilon Hufnagel (Lepidoptera: Noctuidae). Egyptian Journal of Biological Pest Control, 28(1), 82.
  • Yuksel, E., & Canhilal, R. (2019). Isolation, identification, and pathogenicity of entomopathogenic nematodes occurring in Cappadocia Region, Central Turkey. Egyptian Journal of Biological Pest Control, 29(1), 40.

Azadirachtin ve Spinosadın Bazı Yerel Entomoapatojen Nematod İzolatlarının Canlılığı ve Virülensliği Üzerine Etkileri

Yıl 2019, Cilt: 5 Sayı: 2, 280 - 285, 13.12.2019
https://doi.org/10.24180/ijaws.591287

Öz

Entomopatojen nematodlar (EPN), biyoloijk mücadele
uygulamaları içerisinde çoğu zaman biyopestisitlerle birlikte kullanılmaktadır.
Biyopestisitlerin EPN’lar üzerindeki toksik etkilerinin belirlenmesi, bu
uygulamalardan istenilen başarının elde edilebilmesi için oldukça önemlidir. Bu
çalışmada yaygın bir şekilde kullanılan Nimiks (
40 g L-1 Azadirachtin) ve
Laser (Spinosad
480 g L-1)
biyopestisitlerinin bazı yerel EPN türlerinin (Steinernema feltiae E-76, Heterorhabditis
indica
216-H, S. littorale
MGZ-4-S) hayatta kalma ve virülensliği üzerindeki etkileri 24 ve 48 saatlik
periyotlarda son dönem Galleria
mellonella
larvaları üzerinde laboratuvar koşullarında (25oC±2,
R.H. %65±5) araştırılmıştır. Spinosad ve Azadirachtinin tavsiye edilen en
yüksek uygulama dozlarına doğrudan maruz kalan EPN türleri içerisinde ilk 24
saatlik periyotta en tolerant türün %94 canlılık oranıyla H. indica 216-H olduğu belirlenmiştir. Steinernema littorale MGZ-4-S izolatının Azadirachtine 24 ve 48
saat periyotlarda sırasıyla %11 ve %12 ölüm oranlarıyla en çok duyarlılık
gösteren tür olmuştur. Test edilen EPN türlerinin son dönem G. mellonella larvaları üzerinde meydana
getirdikleri ölüm oranları %80 ile %100 arasında değişmiştir. Elde edilen
veriler, test edilen EPN türlerinin Spinosad ve Azadirachtin ile laboratuvar
şartlarında uyumlu olduğunu göstermektedir.

Kaynakça

  • Bayramoglu. Z., Demir, I., Inan, C., & Demirbag, Z. (2018). Efficacy of native entomopathogenic nematodes from Turkey against the alder leaf beetle, Agelastica alni L.(Coleoptera: Chrysomelidae) under laboratory conditions. Egyptian Journal of Biological Pest Control, 28(1), 17.
  • Bortoluzzi, L., Alves, L.F.A., Alves, V. S., & Holz, N. (2013). Entomopathogenic nematodes and their interaction with chemical insecticide aiming at the control of Banana weevil borer, Cosmopolites sordidus Germar (Coleoptera: Curculionidae). Arquivos do Instituto Biológico, 80(2), 183-192.
  • Canhilal, R., Waeyenberge, L., Toktay, H., Bozbuga, R., Çetintas, R., & Imren, M. (2016). Distribution of Steinernematids and Heterorhabditids (Rhabditida: Steinernematidae and Heterorhabditidae) in the Southern Anatolia Region of Turkey. Egyptian Journal of Biological Pest Control, 26(4).
  • Canhilal, R., Waeyenberge, L., Yüksel, E., Koca, A.S., Deniz, Y., & Imren, M. (2017). Assessment of the Natural Presence of Entomopathogenic Nematodes in Kayseri Soils. Turkey. Egyptian Journal of Biological Pest Control, 27(2).
  • Chavan, S.N., Somasekhar, N., & Katti, G. (2018). Compatibility of entomopathogenic nematode Heterorhabditis indica (Nematoda: Heterorhabditidae) with agrochemicals used in the rice ecosystem. Journal of Entomologu and Zoology Studies, 6(4), 527-532.
  • Karabörklü, S.. Ayvaz, A., Yilmaz, S., Azizoglu, U., & Akbulut, M. (2015). Native entomopathogenic nematodes isolated from Turkey and their effectiveness on pine processionary moth, Thaumetopoea wilkinsoni Tams. International journal of pest management, 61(1), 3-8.
  • Kaya, H.K. (1990). Soil Ecology. In: R. Gauglerr, and H.K. Kaya (Eds.), Entomopathogenic Nematodes in Biological Control (pp. 93-115). Florida, USA: CRC Press. Kaya, H., & Gaugler, R. (1993). Entomopathogenic nematodes. Annual Review of Entomology. CRC Press, USA.
  • Kim, K.H.. Kabir, E., & Jahan, S.A. (2017). Exposure to pesticides and the associated human health effects. Science of the Total Environment. 575, 525-535.
  • Krishnayyaand, P. V., & Grewal, P. S. (2002). Effect of neem and selected fungicides on viability and virulence of the entomopathogenic nematode Steinernema feltiae. Biocontrol Science and Technology, 12(2), 259-266.
  • Koppenhöfer, A. M., & Kaya, H. K. (1998). Synergism of imidacloprid and an entomopathogenic nematode: a novel approach to white grub (Coleoptera: Scarabaeidae) control in turfgrass. Journal of Economic Entomology, 91(3), 618-623.
  • Mahmoud, M. F., Mahfouz, H. M., & KM, M. (2016). Compatibility of entomopathogenic nematodes with neonicotinoids and Azadirachtin insecticides for controlling the black cutworm, Agrotis ipsilon (Hufnagel) in canola plants. International Journal of Research in Engineering and Science, 2(1), 11-18.
  • Morales-Rodriguez, A., & Peck, D. C. (2009). Synergies between biological and neonicotinoid insecticides for the curative control of the white grubs Amphimallon majale and Popillia japonica. Biological control, 51(1), 169-180.
  • Ozdemir, E., & Gozel, U. (2017). Efficiency of Some Plant Essential Oils on Root-Knot Nematode Meloidogyne incognita. Journal of Agricultural Science and Technology A, 7(3), 178-183.
  • Ozdemir, E., & Gozel, U. (2018). Nematicidal Activities of Essential Oils Against Meloidogyne Incognita On Tomato Plant. Fresenius Environmental Bulletin, 27(6), 4511-4517.
  • Parrón, T., Requena, M., Hernández, A. F., & Alarcón, R. (2014). Environmental exposure to pesticides and cancer risk in multiple human organ systems. Toxicology Letters, 230(2), 157-165.
  • Radova, S. (2011). Effect of selected pesticides on survival and virulence of two nematode species. Polish Journal of Environmental Studies, 20(1), 181-185.
  • Raheel, M., Javed, N., Khan, S. A., & Ahmed, S. (2017). Exploiting the biocontrol potential of entomopathogenic nematodes in combination with chemicals against greater wax moth (Galleria mellonella L.). Journal of Animal & Plant Sciences, 27(3).
  • Sabarwal, A., Kumar, K., & Singh, R. P. (2018). Hazardous effects of chemical pesticides on human health-cancer and other associated disorders. Environmental toxicology and pharmacology, 63,103-114.
  • Sabino, P.H.S, F.S. Sales, E.J. Guevara, A. Moino. Jr. and C.C. Filgueiras (2014). Compatibility of entomopathogenic nematodes (Nematoda; Rhabditida) with insecticides used in the tomato crop. Nematoda 1.
  • SPSS. (2013). IBM SPSS Statistics 21.0 for Windows. Armonk, NY.
  • Ulu, T. C., Sadic, B., & Susurluk, I. A. (2016). Effects of different pesticides on virulence and mortality of some entomopathogenic nematodes. Invertebrate Survival Journal, 13(1), 111-115.
  • Yüksel, E., Açıkgöz, Ç., Demirci, F., & Muștu, M. (2017). Effects of the entomopathogenic fungi, Beauveria bassiana, Isaria farinosa and Purpureocillium lilacinum, on eggs of Tuta absoluta (Meyrick)(Lepidoptera: Gelechiidae). Türkiye Biyolojik Mücadele Dergisi, 8(1), 39-47.
  • Yuksel, E., Taskesen, Y. E., Erarslan, D., & Canhilal, R. (2018). Effectiveness of different entomopathogenic nematode species against the variegated cutworm, Peridroma saucia (Hubner)(Lepidoptera: Noctuidae). Egyptian Journal of Biological Pest Control, 28(1), 8.
  • Yuksel, E., & Canhilal, R. (2018). Evaluation of local isolates of entomopathogenic nematodes for the management of black cutworm, Agrotis ipsilon Hufnagel (Lepidoptera: Noctuidae). Egyptian Journal of Biological Pest Control, 28(1), 82.
  • Yuksel, E., & Canhilal, R. (2019). Isolation, identification, and pathogenicity of entomopathogenic nematodes occurring in Cappadocia Region, Central Turkey. Egyptian Journal of Biological Pest Control, 29(1), 40.
Toplam 25 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Bölüm Bitki Koruma
Yazarlar

Ebubekir Yüksel 0000-0002-6982-5874

Ramazan Canhilal 0000-0002-0753-0077

Mustafa İmren 0000-0002-7217-9092

Yayımlanma Tarihi 13 Aralık 2019
Gönderilme Tarihi 12 Temmuz 2019
Kabul Tarihi 17 Eylül 2019
Yayımlandığı Sayı Yıl 2019 Cilt: 5 Sayı: 2

Kaynak Göster

APA Yüksel, E., Canhilal, R., & İmren, M. (2019). Azadirachtin ve Spinosadın Bazı Yerel Entomoapatojen Nematod İzolatlarının Canlılığı ve Virülensliği Üzerine Etkileri. Uluslararası Tarım Ve Yaban Hayatı Bilimleri Dergisi, 5(2), 280-285. https://doi.org/10.24180/ijaws.591287

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