Distribution and Genetic diversity of Prune dwarf virus (PDV) in Peach Growing Areas in Bursa and Bilecik Provinces

Yıl 2020, Cilt: 6 Sayı: 1, 66 - 74, 25.04.2020

Ali Çelık , Filiz Ertunç

https://doi.org/10.24180/ijaws.663272

Cited By: 3

Öz

Prune dwarf virus (PDV) is one of the important
viral pathogens of stone fruits. In this study, PDV infection
was investigated extensively for the first time in Bursa and Bilecik provinces,
which have large peach production areas of Turkey. Four hundred-sixty peach
leaves showing viral symptoms were subjected to DAS-ELISA test and 19 samples (%4.13)
were found to be infected with PDV. As a result of RT-PCR studies,
862 bp length nucleotide sequences of the coat protein region of the virus
genome were obtained and compared with reference isolates in the GenBank.
Sequence comparisons showed 87.77-97.56% nucleotide identity. The genetic relationship
was determined by phylogenetic analysis of Turkey and world isolates of PDV.
The results of the study showed that there was no phylogenetic relationship
between geographic origin and PDV isolates from different Prunus species. As a
result of this study, nucleotide sequences of the coat protein regions of PDV
isolates from peach were obtained for the first time in our country.

Anahtar Kelimeler

Bursa, Bilecik, peach, PDV, infection

Bursa ve Bilecik İlleri Şeftali Yetiştiriciliği Yapılan Alanlarda Prune dwarf virus (PDV) Yaygınlığı ve Genetik Çeşitliliği

Öz

Prune dwarf virus (PDV), sert çekirdekli meyve
ağaçlarının önemli viral patojenleri arasında yer almaktadır. Bu çalışmada Türkiye’nin önemli
şeftali üretiminin yapıldığı Bursa ve Bilecik illerinde ilk kapsamlı PDV enfeksiyonu
araştırılmıştır. 460 viral simptom şüphesi ile toplanan şeftali yaprağı
DAS-ELISA testine tabi tutulmuş ve 19 örneğin (%4.13) PDV ile enfekteli olduğu
tespit edilmiştir. Yapılan RT-PCR çalışmaları sonucunda virüsün kılıf protein
bölgesine ait 862 bp uzunluğunda nükleotid dizileri elde edilmiş, elde edilen
diziler GenBank’ta kayıtlı Türkiye ve dünya PDV referans izolatları ile
karşılaştırılmıştır. Yapılan BLASTn analizi sonucunda PDV şeftali Türkiye
izolatları, referans PDV sekans verileri ile nükleotid düzeyinde %87.77-97.56
arasında benzerlik göstermiştir. Nükleotid dizi bilgisi elde edilen izolatların
filogenetik analiz çalışmaları ile, dünya ve Türkiye izolatları ile ilişkisi
belirlenmiştir. Araştırma bulguları coğrafik orijin ve farklı Prunus türlerinden izole edilen PDV
izolatları arasında herhangi bir filogenetik ilişkinin olmadığını göstermiştir.
Çalışma sonucunda ilk kez ülkemizde şeftali PDV izolatlarının kılıf protein
bölgesine ait nükleotid dizileri elde edilmiştir. 

Anahtar Kelimeler

Bursa, Bilecik, şeftali, PDV, enfeksiyon

Kaynakça

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