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Çoklu Bakterilerle Oluşturulmuş Farklı Biyolojik Gübre Formulasyonlarının Pazar 20 Çay Klonunda Yaprak Enzim Aktivitesine Etkileri

Yıl 2021, Cilt: 7 Sayı: 3, 373 - 387, 30.12.2021
https://doi.org/10.24180/ijaws.982894

Öz

Bu çalışma, 2014-2015 yıllarında potlarda, Pazar-20 çay klonuna ait 2 yaşlı fidanlarda yürütülmüştür. Asidik çay topraklarından izole edilerek tanılanan ve karakterize edilip tüm laboratuvar testleri yapılan, biyolojik gübre adaylarından 12 farklı bakteri izolatının (Bacillus atrophaeusRC11, Bacillus megateriumRC07, Bacillus megaterium42/4, Bacillus megaterium21/3, Pseudomonas fluorescen RC77, Pseudomonas fluorescens8/4, Pseudomonas fluorescens8/ 6, Pseudomonas fluorescens9/7, Bacillus subtilisRC63, Bacillus subtilis39/3, Bacillus subtilis36/10, Bacillus subtilisRC521) üçerli kombinasyonları ile oluşturulmuş biyolojik gübre formulasyonları ile 7 farklı taşıyıcının (K-çay kompostu, T-torf, P-perlit, L-leonardit, Z-zeolit, V-vermikülit ve S-likit taşıyıcı formulasyonu) birlikte uygulanmalarının çay yapraklarının enzim aktivitelerine (Peroksidaz-PO, Polifenol oksidaz- PPO, Üreaz, 5-Dehidroksişikimat Redüktaz, Alkol dehidrogenaz-ADH, Glikoz 6 fosfat dehidrogenaz-G6PD, 6 Fosfo glukonat dehidrogenaz-6PGD, Glutatyon redüktaz-GR, Glutatyon S-transferaz-GST) etkileri belirlenmiştir. Araştırmada kullanılan tüm bakteriyel formulasyonlar, çay yaprağındaki enzim aktivitelerini kontrole kıyasla farklı oranlarda olumlu yönde etkilemiştir. Bu etkinlik istatistiki olarak önemli bulunmuştur. Ayrıca, yapılan değerlendirmelerde, her iki yılda da (2014-2015) taşıyıcıların da etkinliğinin önemli olduğu, likit formüllü taşıyıcı ile torf olarak kullanılan taşıyıcıların en yüksek etkiye sahip oldukları belirlenmiştir.

Destekleyen Kurum

TÜBİTAK

Proje Numarası

TOVAG; 112 O 313 project

Teşekkür

Atatürk Çay ve Bahçe Kültürleri Araştırma Enstitüsü RİZE

Kaynakça

  • Angelini, R., Manes, F., & Federico, R. (1990). Spatial and functional correlation between diamine-oxidase and peroxidase activities and their dependence upon deetiolation and wounding in chick-pea stems. Planta, 182, 89-96.
  • Beutler, E. (1984). Red cell metabolism. Manual of biochemical methods. Third edition, Grune Stratton, Inc. Orlando, FL 32887, London.
  • Bhattacharyya, C., Banerjee, S., Acharya, U., Mitra, A., Mallick, I., Haldar, A., Haldar, S., Ghosh, A., & Ghosh, A. (2020). Evaluation of plant growth promotion properties and induction of antioxidative defense mechanism by tea rhizobacteria of Darjeeling, India. Scientific Reports 10,15536.
  • Bradford, M. M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72, 248-251.
  • Carlberg, I., & Mannervik, B. (1985). Glutathione reductase assay. In Methods in Enzymol, FL.113, 484-495. Orlando, Academic Press.
  • Chakraborty, U., Chakraborty, B. N., Basnet, M., & Chakraborty, A. P. (2009). Evaluation of Ochrobactrum anthropi TRS-2 and its talc based formulation for enhancement of growth of tea plants and management of brown root rot disease. Journal Applied Microbiology 107, 625–634.
  • Chakraborty, A. P. C., & Chakraborty, U. (2015). Bacillus megaterium from tea rhizosphere promotes growth and induces systemic resistance in tea against Sclerotium rolfsii. Indian Phytopathology 68, 237–247.
  • Cernava, T., Chen, X., Krug, L., Li, H., Yang, M., & Berg, G. (2019). Tea leaf microbiome shows specifc responses to chemical pesticides and biocontrol applications. Science Total Environment, 667, 33–40.
  • Cakmakci, R., Donmez, M. F., Erturk, Y., Erat, M., Haznedar, A., & Sekban, R. (2010). Diversity and metabolic potential of culturable bacteria from the rhizosphere of Turkish tea grown in acidic soils. Plant and Soil, 332, 299-318.
  • Çakmakçı, R., Ertürk, Y., Atasever, A., Ercişli, S., Şentürk, M., Erat, M., Haznedar, A., & Sekban, R. (2011). The use of plant growth promoting rhizobacteria for organic tea production in Turkey. Proceedings of Tea-Organic-Low Carbon International Symposium, 89-97, 6-9 June, 2011, Guangyuan/China.
  • Çakmakçı, R. (2016). Screening of multi-trait rhizobacteria for Improving the growth, enzyme activities, and nutrient uptake of tea (Camellia sinensis). Communications in Soil Science and Plant Analysis, 47(13–14), 1680–1690.
  • Çakmakçı, R., Ertürk, Y., Varmazyari, A., Atasever, A., Kotan R., Haliloğlu, K., Erat M., Türkyılmaz K., Sekban R., & Haznedar A. (2017). The effect of bacteria-based formulations on tea (Camellia sinensis L.) growth, yield, and enzyme activities. Annals of Warsaw University of Life Sciences – SGGW, Horticulture and Landscape Architecture, 38, 5–18.
  • Cakmakçı, R. (2019). The variability of the predominant culturable plant growth promoting rhizobacterial diversity in the acidic tea rhizosphere soils in the Eastern Black Sea Region. Alinteri Journal of Agricultural Science 34 (2), 175-181.
  • Çalıkoğlu, E., & Bayrak, A. (2009). Çay işleme sırasında aroma maddelerindeki değişim. Gıda, 34 (2), 115-119.
  • Dutta, J., Handique, P. J., & Takur, D. (2015). Assessment of culturable tea rhizobacteria isolated from tea estates of assam, india for growth promotion in commercial tea cultivars. Frontiers Microbiology, 6, 1252.
  • Ertürk Y., Ercisli S., Sengül M., Eser Z., Haznedar A., & Turan M. (2010). Seasonal variation of total phenolic antioxidant activity and minerals in fresh tea shoots Camellia sinensis var Sinensis. Pakistan Journal of Pharmaceutical Sciences, 23 (1), 69-74.
  • Ertürk, Y., Çakmakçı, R., Dönmez, M. F., Sekban R., & Haznedar A. (2011). Doğu Karadeniz Bölgesi asidik topraklarından izole edilmiş bazı bitki büyümesini teşvik edici rizobakterilerin (PGPR) çay bitkisinin fidan gelişimi aşamasında kullanım olanaklarının araştırılması. Türkiye VI. Ulusal Bahçe Bitkileri Kongresi, Şanlıurfa.
  • Ertürk Y., Çakmakçı Y., Sekban R., & Haznedar A. (2014). Biological fertilizer researches for organic tea culture in Turkey (Period: 2007- 2013). International Mesopotamia Agriculture Congress, Diyarbakır, Turkey.
  • Habig, W. H., Pabst, M. J., & Jakoby, W. B. (1974). Glutathione S-transferase: the first enzymalic step in mercaptric acid formation. Journal of Biological Chemistry, 249, 7130-7139.
  • Hatanaka, A., Kajivara, T., Tomohiro, S., & Yamashita, H. (1974). Alcohol dehydrogenase from Thea sinensis seeds. Agricultural and Biological Chemistry, 38(10), 1835-1844.
  • Kacar, B. (2010). Çay Bitkisi Biyokimyası, Gübrelenmesi, İşlenme Teknolojisi. Nobel Yayın No: 1549.
  • Lee, P. M., Lee, K., & Karim, M. I. A. (1991). Biochemical studies of cocoa bean polyphenol oxidase. Journal of the Science and Food Agriculture, 55, 251-260.
  • Mei, X., Lin, D. H., Xu, Y., Wu, Y. Y., You-Ying Tu, Y. Y. (2009). Effects of phenanthrene on chemical composition and enzyme activity in fresh tea leaves. Food Food Chemistry, 115, 569–573.
  • Mishra, A. K., Morang, P., Deka, M., Nishanth Kumar, S., & Dileep Kumar, B. S. (2014). Plant growth-promoting rhizobacterial strainmediated induced systemic resistance in tea (Camellia sinensis (L) O. Kuntze) through defense-related enzymes against brown root rot and charcoal stump rot. Applied Biochemical Biotechnology 174, 506–521.
  • Ramkumar, S., Suresh-kumar, P., Sudhakar, G., Anitha, J., Geetha, S., Mohankumar, P., Kanniappan, V., & Gopalakrishnan, M. (2015). Biochemical and molecular analysis of Camellia sinensis (L.) O. Kuntze tea from the selected P/11/15 clone. Journal of Genetic Engineering and Biotechnology. 14, 69-75.
  • Sanderson, G. W. (1966). 5-Dehydroshikimate reductase in the tea plant (Camellia sinensis L.) properties and distribution. Biochemical Journal, 98, 248-252.
  • Saravanakumar, D., Vijayakumar, C., Kumar, N., & Samiyappan, R. (2007). PGPR-induced defense responses in the tea plant against blister blight disease. Crop Protection, 26(4), 556-565.
  • Smith, M., Hopkinson, D. A., & Harris, H. (1971). Developmental changes and polymorphism in human alcohol dehydrogenase. Annals of Human Genetics, 34, 251-271.
  • Weatherburn, M. W. (1967). Phenol- hypochlorite reaction for determination of ammonia. Analytical Chemistry, 39, 971-974.

Effects of Different Biological Fertilizers Formulated with Multiple Bacteria and Carriers in Pazar 20 Tea Clone on Leaf Enzyme Activity

Yıl 2021, Cilt: 7 Sayı: 3, 373 - 387, 30.12.2021
https://doi.org/10.24180/ijaws.982894

Öz

This study was performed on 2-year-old seedlings of the Pazar-20 tea clone pots in 2014-2015. Twelve different bacterial isolates (Bacillus atrophaeusRC11, Bacillus megateriumRC07, Bacillus megaterium42/4, Bacillus megaterium21/3, Pseudomonas fluorescensRC77, Pseudomonas fluorescens 8/4, Pseudomonas fluorescens8/6, Pseudomonas fluorescens9/7, Bacillus subtilisRC63, Bacillus subtilis 39/3, Bacillus subtilis36/10, Bacillus subtilisRC521), which were isolated from acidic tea soils and characterized and all laboratory tests were performed. With biological fertilizers formed by triple combinations and 7 different carrier formulations (K-tea compost, T-peat, P-perlite, L-leonardite, Z-zeolite, V-vermiculite and S-liquid carrier formulation) on the enzyme activities (peroxidase-POD, Polyphenol oxidase-PPO, Urease, 5-Dehydroxyshikimate reductase, Alchol dehydrogenase-ADH, Glucose 6-phosphate dehydrogenase-G6PD, 6-phosphogluconate dehydrogenase-6PGD, Glutathione reductase-GR and Glutathione S-transferase-GST) of tea leaves were determined. All bacterial formulations used in the study positively affected the enzyme activities in the tea leaves at different rates compared to the control. This activity was found to be statistically significant. Additionally, it was determined that the efficiency of the carrier was important in both years (2014-2015) statistically, liquid formula and peat carriers had the highest effect.

Proje Numarası

TOVAG; 112 O 313 project

Kaynakça

  • Angelini, R., Manes, F., & Federico, R. (1990). Spatial and functional correlation between diamine-oxidase and peroxidase activities and their dependence upon deetiolation and wounding in chick-pea stems. Planta, 182, 89-96.
  • Beutler, E. (1984). Red cell metabolism. Manual of biochemical methods. Third edition, Grune Stratton, Inc. Orlando, FL 32887, London.
  • Bhattacharyya, C., Banerjee, S., Acharya, U., Mitra, A., Mallick, I., Haldar, A., Haldar, S., Ghosh, A., & Ghosh, A. (2020). Evaluation of plant growth promotion properties and induction of antioxidative defense mechanism by tea rhizobacteria of Darjeeling, India. Scientific Reports 10,15536.
  • Bradford, M. M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72, 248-251.
  • Carlberg, I., & Mannervik, B. (1985). Glutathione reductase assay. In Methods in Enzymol, FL.113, 484-495. Orlando, Academic Press.
  • Chakraborty, U., Chakraborty, B. N., Basnet, M., & Chakraborty, A. P. (2009). Evaluation of Ochrobactrum anthropi TRS-2 and its talc based formulation for enhancement of growth of tea plants and management of brown root rot disease. Journal Applied Microbiology 107, 625–634.
  • Chakraborty, A. P. C., & Chakraborty, U. (2015). Bacillus megaterium from tea rhizosphere promotes growth and induces systemic resistance in tea against Sclerotium rolfsii. Indian Phytopathology 68, 237–247.
  • Cernava, T., Chen, X., Krug, L., Li, H., Yang, M., & Berg, G. (2019). Tea leaf microbiome shows specifc responses to chemical pesticides and biocontrol applications. Science Total Environment, 667, 33–40.
  • Cakmakci, R., Donmez, M. F., Erturk, Y., Erat, M., Haznedar, A., & Sekban, R. (2010). Diversity and metabolic potential of culturable bacteria from the rhizosphere of Turkish tea grown in acidic soils. Plant and Soil, 332, 299-318.
  • Çakmakçı, R., Ertürk, Y., Atasever, A., Ercişli, S., Şentürk, M., Erat, M., Haznedar, A., & Sekban, R. (2011). The use of plant growth promoting rhizobacteria for organic tea production in Turkey. Proceedings of Tea-Organic-Low Carbon International Symposium, 89-97, 6-9 June, 2011, Guangyuan/China.
  • Çakmakçı, R. (2016). Screening of multi-trait rhizobacteria for Improving the growth, enzyme activities, and nutrient uptake of tea (Camellia sinensis). Communications in Soil Science and Plant Analysis, 47(13–14), 1680–1690.
  • Çakmakçı, R., Ertürk, Y., Varmazyari, A., Atasever, A., Kotan R., Haliloğlu, K., Erat M., Türkyılmaz K., Sekban R., & Haznedar A. (2017). The effect of bacteria-based formulations on tea (Camellia sinensis L.) growth, yield, and enzyme activities. Annals of Warsaw University of Life Sciences – SGGW, Horticulture and Landscape Architecture, 38, 5–18.
  • Cakmakçı, R. (2019). The variability of the predominant culturable plant growth promoting rhizobacterial diversity in the acidic tea rhizosphere soils in the Eastern Black Sea Region. Alinteri Journal of Agricultural Science 34 (2), 175-181.
  • Çalıkoğlu, E., & Bayrak, A. (2009). Çay işleme sırasında aroma maddelerindeki değişim. Gıda, 34 (2), 115-119.
  • Dutta, J., Handique, P. J., & Takur, D. (2015). Assessment of culturable tea rhizobacteria isolated from tea estates of assam, india for growth promotion in commercial tea cultivars. Frontiers Microbiology, 6, 1252.
  • Ertürk Y., Ercisli S., Sengül M., Eser Z., Haznedar A., & Turan M. (2010). Seasonal variation of total phenolic antioxidant activity and minerals in fresh tea shoots Camellia sinensis var Sinensis. Pakistan Journal of Pharmaceutical Sciences, 23 (1), 69-74.
  • Ertürk, Y., Çakmakçı, R., Dönmez, M. F., Sekban R., & Haznedar A. (2011). Doğu Karadeniz Bölgesi asidik topraklarından izole edilmiş bazı bitki büyümesini teşvik edici rizobakterilerin (PGPR) çay bitkisinin fidan gelişimi aşamasında kullanım olanaklarının araştırılması. Türkiye VI. Ulusal Bahçe Bitkileri Kongresi, Şanlıurfa.
  • Ertürk Y., Çakmakçı Y., Sekban R., & Haznedar A. (2014). Biological fertilizer researches for organic tea culture in Turkey (Period: 2007- 2013). International Mesopotamia Agriculture Congress, Diyarbakır, Turkey.
  • Habig, W. H., Pabst, M. J., & Jakoby, W. B. (1974). Glutathione S-transferase: the first enzymalic step in mercaptric acid formation. Journal of Biological Chemistry, 249, 7130-7139.
  • Hatanaka, A., Kajivara, T., Tomohiro, S., & Yamashita, H. (1974). Alcohol dehydrogenase from Thea sinensis seeds. Agricultural and Biological Chemistry, 38(10), 1835-1844.
  • Kacar, B. (2010). Çay Bitkisi Biyokimyası, Gübrelenmesi, İşlenme Teknolojisi. Nobel Yayın No: 1549.
  • Lee, P. M., Lee, K., & Karim, M. I. A. (1991). Biochemical studies of cocoa bean polyphenol oxidase. Journal of the Science and Food Agriculture, 55, 251-260.
  • Mei, X., Lin, D. H., Xu, Y., Wu, Y. Y., You-Ying Tu, Y. Y. (2009). Effects of phenanthrene on chemical composition and enzyme activity in fresh tea leaves. Food Food Chemistry, 115, 569–573.
  • Mishra, A. K., Morang, P., Deka, M., Nishanth Kumar, S., & Dileep Kumar, B. S. (2014). Plant growth-promoting rhizobacterial strainmediated induced systemic resistance in tea (Camellia sinensis (L) O. Kuntze) through defense-related enzymes against brown root rot and charcoal stump rot. Applied Biochemical Biotechnology 174, 506–521.
  • Ramkumar, S., Suresh-kumar, P., Sudhakar, G., Anitha, J., Geetha, S., Mohankumar, P., Kanniappan, V., & Gopalakrishnan, M. (2015). Biochemical and molecular analysis of Camellia sinensis (L.) O. Kuntze tea from the selected P/11/15 clone. Journal of Genetic Engineering and Biotechnology. 14, 69-75.
  • Sanderson, G. W. (1966). 5-Dehydroshikimate reductase in the tea plant (Camellia sinensis L.) properties and distribution. Biochemical Journal, 98, 248-252.
  • Saravanakumar, D., Vijayakumar, C., Kumar, N., & Samiyappan, R. (2007). PGPR-induced defense responses in the tea plant against blister blight disease. Crop Protection, 26(4), 556-565.
  • Smith, M., Hopkinson, D. A., & Harris, H. (1971). Developmental changes and polymorphism in human alcohol dehydrogenase. Annals of Human Genetics, 34, 251-271.
  • Weatherburn, M. W. (1967). Phenol- hypochlorite reaction for determination of ammonia. Analytical Chemistry, 39, 971-974.
Toplam 29 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Bahçe Bitkileri Yetiştirme ve Islahı
Bölüm Bahçe Bitkileri
Yazarlar

Yaşar Ertürk 0000-0003-2525-0260

Ramazan Cakmakcı 0000-0002-1354-1995

Meral Kutlu 0000-0002-3288-1266

Proje Numarası TOVAG; 112 O 313 project
Yayımlanma Tarihi 30 Aralık 2021
Gönderilme Tarihi 16 Ağustos 2021
Kabul Tarihi 6 Kasım 2021
Yayımlandığı Sayı Yıl 2021 Cilt: 7 Sayı: 3

Kaynak Göster

APA Ertürk, Y., Cakmakcı, R., & Kutlu, M. (2021). Effects of Different Biological Fertilizers Formulated with Multiple Bacteria and Carriers in Pazar 20 Tea Clone on Leaf Enzyme Activity. Uluslararası Tarım Ve Yaban Hayatı Bilimleri Dergisi, 7(3), 373-387. https://doi.org/10.24180/ijaws.982894

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