Investigation of the Effect of Magnesium Restriction on Glucose Transport in Schizosaccharomyces pombe

Öz

Magnesium is vital for many biological processes such as energy metabolism, nucleic acid, and protein synthesis, signal transduction, and cell division. Impairment of magnesium homeostasis is associated with many diseases, especially cardiovascular diseases, hypertension, type 2 diabetes, and cancer. More than 300 million people worldwide struggle with type 2 diabetes, and this number is growing exponentially. Clinical studies have shown that serum magnesium levels were decreased in patients with type 2 diabetes and that magnesium supplementation has positive effects on glucose metabolism. In this study, glucose consumption and the expression level of glucose transporters (ght1, ght2, ght5) were investigated in magnesium transport-restricted model cell of Schizosaccharomyces pombe yeast, which is similar to mammalian cells in terms of biological processes and genetic mechanisms. In mutant strain with limited magnesium transport, glucose consumption increased due to the increase in magnesium added to the medium. The expression level of ght1, ght2, one of the glucose transporters, increased in 30 mM Mg2+ supplemented medium, decreased at higher magnesium concentration (75 mM), where optimum growth was observed for the strain, and there was no significant change in the expression level of ght5. Our findings indicate that the glucose transporters ght1 and ght2 are regulated by a different mechanism than ght5

Anahtar Kelimeler

• magnesium deficiency
• glucose transport
• Schizosaccharomyces pombe

Schizosaccharomyces pombe’ de Magnezyum Kısıtlamasının Glukoz Transportu Üzerine Etkisinin Araştırılması

Öz

Magnezyum, enerji metabolizması, nükleik asit ve protein sentezi, sinyal iletimi, hücre bölünmesi gibi birçok biyolojik süreç için hayati önem taşır. Magnezyum homeostasisinin bozulması, kardiyovasküler hastalıklar, hipertansiyon, tip 2 diyabet ve kanser başta olmak üzere çok sayıda hastalıkla ilişkilendirilmiştir. Dünya çapında 300 milyondan fazla insan tip 2 diyabet ile mücadele etmektedir ve bu sayı katlanarak artmaktadır. Klinik çalışmalar, tip 2 diyabetli hastalarda serum magnezyum seviyesinin düştüğünü ve magnezyum takviyesinin glukoz metabolizması üzerine olumlu etkileri olduğunu göstermiştir. Bu çalışmada, biyolojik süreçler ve genetik mekanizmalar bakımından memeli hücreleriyle benzerlik gösteren Schizosaccharomyces pombe mayasının magnezyum transportu kısıtlı mutant suşunda glukoz tüketimi ve glukoz taşıyıcılarının (ght1, ght2, ght5) anlatım seviyeleri araştırılmıştır. Magnezyum transportu kısıtlı olan mutant suşta, besi ortamına ilave edilen magnezyum artışına bağlı olarak glukoz tüketimi artmıştır. Glukoz taşıyıcılarından ght1, ght2 nin anlatım düzeyi, 30 mM Mg+2destekli ortamda artmış, suş için optimum üremenin görüldüğü daha yüksek magnezyum konsantrasyonunda (75 mM) azalmış, ght5’in anlatım düzeyinde ise anlamlı bir değişim bulunmamıştır. Bulgularımız, glukoz taşıyıcılarından ght1 ve ght2’ nin ght5’ ten farklı bir mekanizma ile düzenlendiğini işaret etmektedir.

Anahtar Kelimeler

• magnezyum eksikliği
• glukoz transportu
• Schizosaccharomyces pombe

Kaynakça

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