Assessing of antiradical and antioxidant activities of -carnitine, L-carnitine, Coenzyme Q10 and Beta Carotene dietary supplements

Öz

In this study, common tests such as 2,2-diphenyl-l-picrylhydrazyl (DPPH) assay, hydrogen peroxide (H2O2) and superoxide anion (O·̄2 ) radical scavenging activity were exerted to measure the antioxidant activities of L-carnitine, Coenzyme Q10 (Co-Q10) and β-carotene dietary supplements. Antiradical and antioxidant capacities were evaluated comparatively with these tests. The highest free radical scavenging activity was observed in β-carotene (H2O2 73%; O·̄2 69%), while the lowest activity was recorded in L-carnitine (H2O2 59%; O·̄2 67%). The radical scavenging activity of these dietary supplements was discovered to be significantly different from each other (P < 0,05). The antiradical activities of these dietary supplements were measured colorimetrically based on their DPPH radical scavenging ability. The effective concentrations (EC50) values of β-carotene, Co-Q10, L-carnitine and methanol solutions were measured as 10.060, 28.990 and 146.682 μg/mL after 30 min, respectively. These results demonstrate the potent antioxidant and antiradical activities of all three dietary supplements compared to ascorbic acid. It can only be said that the antiradical activity of L-carnitine is lower than the others, suggesting that this may be due to kinetic behavior.

Anahtar Kelimeler

• Antioxidant
• β-carotene
• DPPH
• Coenzyme Q10
• L-carnitine

-karnitin, Koenzim Q10 ve Beta Karoten diyet takviyelerinin antioksidan ve antiradikal aktivitelerinin değerlendirilmesi

Öz

Bu çalışmada, L-karnitin, Koenzim Q10 (Co-Q10) ve β-karoten diyet takviyelerinin antioksidan aktivitelerini ölçmek için 2,2-difenil-l-pikrilhidrazil (DPPH) deneyi, süperoksit anyon (O·̄2) radikali ve hidrojen peroksit (H2O2) temizleme aktivitesi gibi yaygın testler kullanılmıştır. Bu testler ile antiradikal ve antioksidan kapasiteleri karşılaştırılarak değerlendirilmiştir. En yüksek serbest radikal temizleme aktivitesi β-karoten de (H2O2 %73; O·̄2 %69) gözlenirken, en düşük aktivite L-karnitin (H2O2 %59; O·̄2 %67) kaydedildi. Bu diyet takviyelerinin radikalleri temizleme aktivitesi birbirinden oldukça farklı olduğu kaydedilmiştir (P < 0.05). Bu diyet takviyelerinin antiradikal aktiviteleri kararlı DPPH radikalini temizleme yeteneği, kolorimetrik olarak ölçüldü. β-karotenin Co-Q10, L-karnitin ve metanol çözeltilerinin etkili konsantrasyonları (EC50) değerleri 30 dk sonra sırasıyla 10.060, 28,990 ve 146,682 μg/mL olarak ölçülmüştür. Bu sonuçlar, her üç diyet takviyesinin askorbik asit ile kıyaslandığıında güçlü antioksidan ve antiradikal aktivitelerini göstermektedir. Sadece, L-karnitinin antiradikal aktivitesinin diğerlerinde göre daha düşük olduğu söylenebilir, bu durumun ise kinetik davaranışa bağlı olabileceği akla getirmiştir.

Anahtar Kelimeler

• Antioksidan
• β-karoten
• DPPH
• Koenzim Q10
• L-karnitin

Kaynakça

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