Gadolinyumun Tatlı Su Omurgasızı Dreissena polymorpha Üzerindeki Biyokimyasal Etkileri

Yıl 2021, , 229 - 236, 20.07.2021

Osman Serdar , Numan Yıldırım , Şule Tatar , Nuran Cikcikoglu Yildirim

https://doi.org/10.29132/ijpas.873218

Cited By: 2

Öz

Bu çalışma, metabolik ve oksidatif stres biyobelirteçlerinin analizi yoluyla, su kirliliğinin iyi bir biyoindikatörü olarak kabul edilen tatlı su omurgasızı Dreissena polymorpha üzerindeki Gadolinyum (Gd) toksisitesini değerlendirmeyi amaçlamaktadır. Öncelikle çalışmamızda Gd’nin D. polymorpha üzerindeki LC50 değeri belirlenmiştir. D. polymorpha, kontrollü koşullar altında 24 ve 96 saatlik bir süre boyunca üç letal olmayan Gd konsantrasyonuna (1/20, 1/10 ve 1/5 LC50 değerine) maruz bırakılmıştır. TBARS ve CYP1A1 seviyeleri ELISA kiti kullanılarak belirlenmiştir. CYP1A1 ve TBARS düzeylerinin tüm gruplarda 24. ve 96. saatte kontrol grubuna göre arttığı görülmüştür. Bu sonuçlar, Gd'nin tatlı su omurgasızları, D. polymorpha için bir risk olarak kabul edilebileceğini ve bu nedenle alıcı ortamlara deşarjının denetlenmesi gerektiğini göstermektedir.

Anahtar Kelimeler

CYP1A1, Dreissena polymorpha, Gadolinyum

Biochemical Effects of Gadolinium on a Freshwater Invertebrate Dreissena polymorpha

Öz

This study aims to assess the toxicity of Gadolinium (Gd) on a freshwater invertebrate Dreissena polymorpha, considered a good bioindicator of aquatic pollution, through the analysis of metabolic and oxidative stress markers. Firsly, LC50 value of Gd for D. polymorpha was determined in our study. D. polymorpha were exposed to the three sublethal Gd concentrations (1/20, 1/10 and 1/5 of LC50 value) for a period of 24 and 96 hours under controlled conditions. TBARS and CYP1A1 levels were determined by using ELISA kit. The CYP1A1 and TBARS levels was found to be increased in the all groups at 24 and 96 h When compared to control group. These results show that Gd can be considered a risk for freshwater invertebrate, D. polymorpha and as such its discharge in the receiving environment should be supervised.

Anahtar Kelimeler

CYP1A1, Dreissena polymorpha, Gadolinyum, TBARS

Kaynakça

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