Tunceli Yöresindeki At Dışkısı Örneklerinden İzole Edilen Anaerobik Gut Funguslarının (Piromyces spp.) Saf Kültürdeki Fibrolitik Aktiviteleri ve Moleküler Tanımlanması

Yıl 2024, Cilt: 10 Sayı: 2, 590 - 603, 31.12.2024

Bülent Kar

https://doi.org/10.29132/ijpas.1566759

Öz

Bu çalışma, Türkiye'nin Tunceli ilindeki at dışkılarından anaerobik gut funguslarının (AGF) izolasyonu, karakterizasyonu ve enzim özelliklerine odaklanmaktadır. At dışkısından Piromyces cinsine ait iki izolat MUBAM_F1 ve MUBAM_F2, morfolojik ve moleküler teknikler kullanılarak izole edilmiş ve kısmen tanımlanmıştır. ITS bölgesinin Blast analizi, her iki izolatında Piromyces cinsi içerisinde bir tür olduğu doğrulamıştır (sırasıyla %98.24 ve %98.87 benzerlik). Enzimatik analizler, her iki izolat için de önemli ksilanaz ve karboksimetil selülaz aktivitesinin olduğunu göstermiştir. MUBAM_F1, toplam 47.13±0.10 µmol/dak/ml (spesifik aktivite: 2153.93 µmol/dak/mg protein) ksilanaz aktivitesi gösterirken, toplam 35.56 µmol/dak/ml (spesifik aktivite: 1626.52 µmol/dak/mg protein) karboksimetil selülaz aktivitesi göstermiştir. MUBAM_F2, 57.53±0.08 µmol/dak/ml'de daha yüksek toplam ksilanaz aktivitesi göstermiş (spesifik aktivite: 2630.11 µmol/dak/mg protein) ancak 23.93±1.41 µmol/dak/ml'de daha düşük karboksimetil selülaz aktivitesine (spesifik aktivite: 1098.66 µmol/dak/mg protein) sahip olduğu belirlenmiştir. Çalışmada elde edilen veriler, Piromyces sp. MUBAM_F1 ve MUBAM_F2'nin ksilanaz ve kar-boksimetil selülaz enzimlerinin umut vadeden kaynakları olduğunu göstermektedir.

Anahtar Kelimeler

Anaerobik Gut Funguslar , Piromyces sp. , At , Ksilanaz ve Karboksimetil selülaz

Fibrolytic Activity and Molecular Identification of Anaerobic Gut Fungi (Piromyces spp.) in Pure Culture Samples, Isolated from Local Horse Fae-ces of Tunceli

Öz

This study focuses on the isolation, characterization, and enzyme properties of an-aerobic gut fungi (AGF) isolated from horse feces in Tunceli province, Turkiye. Two Piromyces strains, MUBAM_F1 and MUBAM_F2, isolated from horse feces were identified using morphological and molecular techniques. Blast analysis of the ITS region confirmed that both isolates are belong to the genus Piromyces (98.24% and 98.87% similarity, respectively). Enzymatic analyses showed significant xylanase and carboxymethyl cellulase activities for both isolates. MUBAM_F1 showed total xy-lanase activity of 47.13±0.10 µmol/min/ml (specific activity: 2153.93 µmol/min/mg protein) while total carboxymethyl cellulase activity of 35.56 µmol/min/ml (specific activity: 1626.52 µmol/min/mg protein). MUBAM_F2 was determined to have higher total xylanase activity at 57.53±0.08 µmol/min/ml (specific activity: 2630.11 µmol/min/mg protein) but lower carboxymethyl cellulase activity at 23.93±1.41 µmol/min/ml (specific activity: 1098.66 µmol/min/mg protein). The data obtained in the study indicate that Piromyces sp. MUBAM_F1 and MUBAM_F2 are promising sources of xylanase and carboxymethyl cellulase enzymes.

Anahtar Kelimeler

Anaerobic Gut Fungi , Piromyces sp. , Horse , Xylanase and Carboxymethyl cellulase

Kaynakça

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