Grafen Oksit/Poliimid Temelli Biyosensör Kullanarak Melatonin ve Dopaminin Voltametrik Analizi

Yıl 2020, , 367 - 382, 21.06.2020

Öznur Güngör , Aziz Paşahan , Büşra Aksoy , Süleyman Köytepe , Turgay Seçkin

https://doi.org/10.33715/inonusaglik.736510

Cited By: 2

Öz

Bu araştırmada, melatonin (MET) ve dopaminin (DP) eş zamanlı analizi için, Pt elektrodun modifikasyonu ile grafen oksit/poliimit (GO/PI) kompozit elektrot hazırlanmıştır. İlk olarak, GO/PI kompozit yapısı, 2,6-diaminopiridin temelli PI ve % 5 GO'den sentezlenmiştir. Daha sonra membran olarak elde edilen kompozit yapı elektrot yüzeyi üzerine kaplanmıştır. Hazırlanan GO/PI kompozit elektrodunun MET ve DP yanıtları Diferansiyel Puls Voltametri (DPV) tekniği ile araştırılmıştır. MET ölçümleri için doğrusallık 85- 105 µM (R2=0.9976) konsantrasyon aralığında elde edilmiştir. DP analizi için de doğrusallık 85-105 µM konsantrasyon aralığında izlenmiştir (R2=0.9988). GO/PI ile modifiye edilmiş elektrodun tayin limitleri, MET ve DP için sırasıyla yaklaşık 13.45x10−5 M ve 9.61x10−5 M’dı. Hazırlanan GO/PI kompozit modifiye elektrot, MET ve DP için iyi tekrarlanabilirlik, geniş doğrusal aralık ve hassasiyet sergilemiştir. Elde edilen sonuçlar, GO/PI kompozitinin ortamda ürik asit (UA) varlığında, MET ve DP’i eş zamanlı olarak analiz etmek için mükemmel bir membran olarak kullanılabileceğini göstermiştir.

Anahtar Kelimeler

Dopamin, Elektrokimyasal biyosensör, Grafen oksit/poliimid kompozit

VOLTAMMETRIC ANALYSIS OF MELATONIN AND DOPAMINE BY USING GRAPHENE OXIDE/POLYIMIDE BASED BIOSENSOR

Öz

In this research, for the simultaneous analysis of melatonin (MET) and dopamine (DP), graphene oxide/polyimide (GO/PI) composite electrode was prepared with the modification of Pt electrode. Firstly, GO/PI composite structure was synthesized from 2,6-diaminopyridine based polyimide and 5% GO. Then, the obtained composite structure as the membrane was coated on the electrode surface. MET and DP responses of the prepared GO/PI composite electrode were investigated by Differential Pulse Voltammetry (DPV) technique. Linearity was obtained over a concentration range of 85-105 µM for MET (R2 = 0.9976). For DP analysis, the linearity was also monitored over a concentration range of 85-105 µM (R2=0.9988). The detection limits of GO/PI modified electrode were approximately 13.45×10−5 M and 9.61×10−5 M for MET and DP, respectively. The prepared GO/PI composite modified electrode exhibited good repeatability, wide linear range and sensitivity for MET and DP. The obtained results indicated that while uric acid (UA) is present in the medium, GO/PI composite can be used as an excellent membrane in the design of voltammetric sensors to analyze MET and DP simultaneously.

Anahtar Kelimeler

Electrochemical biosensor, Dopamine, Graphene oxide/polyimide composite

Kaynakça

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