Araştırma Makalesi
PİROL VE ÇOK DUVARLI KARBON NANOTÜP MODİFİYE SENSÖR İLE LEVODOPA VE BENSERAZİD’İN ELEKTROKİMYASAL OLARAK EŞZAMANLI TAYİNİ
Öz
Bu çalışmada, susuz ortamda camsı karbon elektrot (CKE) üzerinde pirolün elektropolimerizasyonu ve çok duvarlı karbon nanotüpün (ÇDKN) modifikasyonu ile elektrokimyasal bir sensör geliştirilmiştir. Polipirol ve ÇDKN temelli elektrot, Parkinson hastalığı tedavisinde kullanılan etken maddeler olan Levodopa ve Benserazid’in elektrokimyasal olarak kantitatif tayini için kullanılmıştır. Modifiye edilmiş sensör üzerindeki farklı parametrelerin etkisi test edilmiş ve optimize edilen yöntem yüksek seçicilik ve hassasiyet göstermiştir. Modifiye sensör, 10 - 50 µM Levodopa ve 100 - 500 µM Benserazid derişim aralıklarında doğrusal yanıtlar vermiştir. Levodopa ve Benserazid için sırasıyla 3.65 µM ve 1.99 µM'lık tayin sınırları elde edilmiştir. Sensörün aktivitesi, girişim yapan türlerden biri olan askorbik asit varlığında başarıyla test edildi. Geliştirilen yöntem, gerçek idrar numunelerinde ve farmasötik dozaj formundaki Levodopa ve Benserazid’in tayininde başarıyla uygulanmıştır.
Anahtar Kelimeler
Destekleyen Kurum
İnönü Üniversitesi Bilimsel Araştırma Projeleri Birimi
Proje Numarası
2013-54
Teşekkür
Bu çalışma İnönü Üniversitesi Bilimsel Araştırma Projeleri Birimi tarafından 2013-54 Proje numarası ile desteklenmiştir.
Kaynakça
- Dinc E, Ustundag O. Chemometric resolution of a mixture containing hydrochlorothiazide and amiloride by absorption and derivative spectrophotometry, Journal of Pharmaceutical and Biomedical Analysis, 2002; 29(1-2): 371-379.
- Ermer J, Miller JH. Method validation in pharmaceutical analysis, Wiley-VCH, Weinheim, 2005.
- Goodman G, Rall TW, Nıes AS, Taylor P. The Pharmacological Basis of Therapeutics, 18th Ed, Pergamon Pres, New York, 1990.
- Hu CG, Wang WL, Liao KJ, Wang YT. Simultaneous measurement of epinephrine and ascorbic acid at the carbon nanotube electrode, Journal of Metastable and Nanocrystalline Materials, 2005; 23: 305-308.
- Liu A, Honma I, Zhou H. Amperometric biosensor based on tyrosinase-conjugated polysacchride hybrid film: Selective determination of nanomolar neurotransmitters metabolite of 3,4-dihydroxyphenylacetic acid (DOPAC) in biological fluid, Biosensors and Bioelectronics, 2005; 21: 809–816.
- Marques KL, Santos JL, Lopes JA, Lıma JL. Simultaneous chemiluminometric determination of levodopa and benserazide in a multi-pumping flow system with multivariate calibration, Analytical Sciences, 2008; 24(8): 985-991.
- Ommaty R. Vademecum Modern İlaç Rehberi, 32ed PelikanYayınları, 2009.
- Ouveria-Caridade C, Pauliukaite R, Brett CMA. Development of electrochemical oxidase biosensors based on carbon nanotube-modified carbon film electrodes for glucose and ethanol, Electrochima Acta, 2008; 53: 6732-6739.
- Özkan SA, Uslu B, Aboul-Enein HY. Analysis of Pharmaceuticals and Biological Fluids Using Modern Electroanalytical Techniques. Critical Reviews in Analytical Chemistry, 2003; 33: 155-181.
- Pan L, Guo Y, Li Z. Simultaneous Determination of Levodopa, Benserazide and 3-O-Methyldopa in Human Serum by LC–MS–MS, Chromatographia, 2010; 72(7-8): 627-633.
- Tsang SC, Chen YK, Harrıs PJF, Green MLH. A simple chemical method of opening and filling carbon nanotubes, Nature, 1994; 372: 159-162.
- Wang JX, Li MX, Shi ZJ, Li NQ, Gu ZN. Direct electrochemistry of cytochrome c at a glassy carbon electrode modified with single-wall carbon nanotubes, Analytical Chemistry, 2002; 74: 1993-1997.
- Wu Z, Zhao H, Xue Y, He Y, Li X, Yuan Z. Poly(pyridine-3-boronic acid)/Multiwalled Carbon Nanotubes Modified Glassy Carbon Electrodes for Simultaneous, Electroanalysis, 2010; 22(19): 2196-2201.
Yıl 2020,
Cilt: 8 Sayı: 2, 336 - 355, 21.06.2020
Öz
In this study, an electrochemical sensor has been developed by modification of the electropolymerized pyrrole and multi-walled carbon nanotube (MWCNT) on the glassy carbon electrode in anhydrous environment. The polypyrrole and MWCNT based electrode have been used for the electrochemical quantitative determination of the active substances Levodopa and Benserazide which are used in the treatment of Parkinson's disease. The effect of different parameters on the modified sensor have been tested and the optimized method showed high selectivity and precision. The modified sensor gave linear responses in the concentration ranges of 10 - 50 µM Levodopa and 100 - 500 µM Benserazide. Determination limits of 3.65 µM and 1.99 µM have been obtained for Levodopa and Benserazide, respectively. The activity of the sensor was successfully tested in the presence of an interference species, ascorbic acid. The developed method has successfully been applied in the determination of Levodopa and Benserazide in real urine samples and pharmaceutical dosage form.
Anahtar Kelimeler
Proje Numarası
2013-54
Kaynakça
- Dinc E, Ustundag O. Chemometric resolution of a mixture containing hydrochlorothiazide and amiloride by absorption and derivative spectrophotometry, Journal of Pharmaceutical and Biomedical Analysis, 2002; 29(1-2): 371-379.
- Ermer J, Miller JH. Method validation in pharmaceutical analysis, Wiley-VCH, Weinheim, 2005.
- Goodman G, Rall TW, Nıes AS, Taylor P. The Pharmacological Basis of Therapeutics, 18th Ed, Pergamon Pres, New York, 1990.
- Hu CG, Wang WL, Liao KJ, Wang YT. Simultaneous measurement of epinephrine and ascorbic acid at the carbon nanotube electrode, Journal of Metastable and Nanocrystalline Materials, 2005; 23: 305-308.
- Liu A, Honma I, Zhou H. Amperometric biosensor based on tyrosinase-conjugated polysacchride hybrid film: Selective determination of nanomolar neurotransmitters metabolite of 3,4-dihydroxyphenylacetic acid (DOPAC) in biological fluid, Biosensors and Bioelectronics, 2005; 21: 809–816.
- Marques KL, Santos JL, Lopes JA, Lıma JL. Simultaneous chemiluminometric determination of levodopa and benserazide in a multi-pumping flow system with multivariate calibration, Analytical Sciences, 2008; 24(8): 985-991.
- Ommaty R. Vademecum Modern İlaç Rehberi, 32ed PelikanYayınları, 2009.
- Ouveria-Caridade C, Pauliukaite R, Brett CMA. Development of electrochemical oxidase biosensors based on carbon nanotube-modified carbon film electrodes for glucose and ethanol, Electrochima Acta, 2008; 53: 6732-6739.
- Özkan SA, Uslu B, Aboul-Enein HY. Analysis of Pharmaceuticals and Biological Fluids Using Modern Electroanalytical Techniques. Critical Reviews in Analytical Chemistry, 2003; 33: 155-181.
- Pan L, Guo Y, Li Z. Simultaneous Determination of Levodopa, Benserazide and 3-O-Methyldopa in Human Serum by LC–MS–MS, Chromatographia, 2010; 72(7-8): 627-633.
- Tsang SC, Chen YK, Harrıs PJF, Green MLH. A simple chemical method of opening and filling carbon nanotubes, Nature, 1994; 372: 159-162.
- Wang JX, Li MX, Shi ZJ, Li NQ, Gu ZN. Direct electrochemistry of cytochrome c at a glassy carbon electrode modified with single-wall carbon nanotubes, Analytical Chemistry, 2002; 74: 1993-1997.
- Wu Z, Zhao H, Xue Y, He Y, Li X, Yuan Z. Poly(pyridine-3-boronic acid)/Multiwalled Carbon Nanotubes Modified Glassy Carbon Electrodes for Simultaneous, Electroanalysis, 2010; 22(19): 2196-2201.
Toplam 13 adet kaynakça vardır.
Ayrıntılar
| Birincil Dil | Türkçe |
|---|---|
| Konular | Klinik Tıp Bilimleri |
| Bölüm | Araştırma Makalesi |
| Yazarlar | |
| Proje Numarası | 2013-54 |
| Yayımlanma Tarihi | 21 Haziran 2020 |
| Gönderilme Tarihi | 16 Nisan 2020 |
| Kabul Tarihi | 25 Mayıs 2020 |
| Yayımlandığı Sayı | Yıl 2020 Cilt: 8 Sayı: 2 |
