Kayısı Yaprak Özütü ile Sentezlenen Gümüş Nanopartiküllerin Antimikrobiyal Potansiyeli

Yıl 2022, Cilt: 10 Sayı: 1, 50 - 57, 10.03.2022

Ayşe Baran , Özfer Yeşilada

https://doi.org/10.33715/inonusaglik.1012011

Cited By: 2

Öz

Metalik nanopartiküller tıpta önemli maddelerdir. Bu partiküller yeşil sentez dahil çeşitli yollarla üretilebilirler. Gümüş nanopartiküllerin bitki özütleri ile üretimi çevre dostu özelliklerinden dolayı yaygın olarak kullanılmaktadır. Bu çalışmada gümüş nanopartiküller biyolojik yöntemle sentezlenmiş ve Kayısı ağacı (Prunus armeniaca) yaprak özütü redükleyici ajan olarak kullanılmıştır. Renk değişimi ve 400-500 nm’de özgül absorbans piki oluşumu AgNP oluşumunun ilk göstergeleri olmuştur. XRD analizi kristal yapıda partikülleri göstermiştir ve elde edilen AgNP’ler ortalama 24 nm boyutunda sferik şekillidir. Nanopartiküllerin çeşitli patojenik mikroorganizmalar üzerine antimikrobiyal aktivite çalışmaları, bu nanopartiküllerin Escherichia coli ve Staphylococcus aureus üzerine kuvvetli antibakteriyel ve Candida albicans’a karşı da kuvvetli antifungal etkisi olduğunu göstermiştir. E. coli, S. aureus ve C. albicans için minimum inhibe edici konsantrasyonlar sırasıyla 0.125, 0.125 ve 0.250 g /mL’dir. Sonuçlar, Prunus armeniaca yaprak özütünün etkili antimikrobiyal aktiviteye sahip AgNP’lerin üretiminde kullanılabileceğini ortaya koymuştur.

Anahtar Kelimeler

Antimikrobiyal, Gümüş nanopartikül, Kayısı, Prunus armeniaca yaprak özütü, Yeşil sentez

Destekleyen Kurum

İnönü Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi

Proje Numarası

2016/111

Teşekkür

Bu çalışma İnönü Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimince Desteklenmiştir. Proje numarası 2016/111.

ANTIMICROBIAL POTENTIAL OF SILVER NANOPARTICLES PRODUCED BY APRICOT LEAF EXTRACT

Öz

Metallic nanoparticles are important substances in medicine. These particles could be prepared by various ways including green synthesis. The production of silver nanoparticles by plant extracts is widely used because of their environmentally friendly properties. In this study, silver nanoparticles have been synthesized by biological method and Apricot tree (Prunus armeniaca) leaf extract was used as reducing agent. The color change and formation of the characteristic absorption peak at 400-500 nm were the first indications of AgNP formation. XRD analysis showed the crystalline particles and obtained AgNPs were generally in spherical shape with the average particle size of 24 nm. Their antimicrobial activity studies against various pathogenic microorganisms showed that they have strong antibacterial activity against Escherichia coli and Staphylococcus aureus and antifungal activity against Candida albicans. The minimum inhibitory concentrations for E. coli, S. aureus and C. albicans were 0.125, 0.125 and 0.250 g/mL, respectively. The results confirmed that Prunus armeniaca leaf extract could be used to produce AgNPs with efficient antimicrobial activity.

Anahtar Kelimeler

Antimicrobial, Apricot, Green Synthesis, Prunus armeniaca leaf extract, Silver nanoparticles

Proje Numarası

2016/111

Kaynakça

• Ali, Z. A., Yahya, R., Sekaran, S. D., Puteh, R. (2016). Green synthesis of silver nanoparticles using apple extract and its antibacterial properties. Advances in Materials Science and Engineering, Volume 2016, Article ID 4102196, 1-6.
• Apohan, E., Yilmaz, U., Yilmaz, O., Serindag, A., Kucukbay, H., Yesilada, O., Baran, Y. (2017). Synthesis, cytotoxic and antimicrobial activities of novel cobalt and zinc complexes of benzimidazole derivatives, Journal of Organometallic Chemistry. 828, 52-58.
• Bao, Z. Q., Cao, J. H., Kang, G. B., Lan, C. Q. (2019). Effects of reaction conditions on light-dependent silver nanoparticle biosynthesis mediated by cell extract of green alga Neochloris oleoabundans. Environmental Science and Pollution Research, 26, 2873-2881.
• Chintamani, R. B., Salunkhe, K. S., Chavan, M. J. (2018). Emerging use of green synthesis silver nanoparticle: An updated review. International Journal of Pharmeceutical Sciences and Research, 9, 4029-4055.
• Dauthal, P., Mukhopadhyay, M. (2013). In-vitro free radical scavenging activity of biosynthesized gold and silver nanoparticles using Prunus armeniaca (apricot) fruit extract. Journal of Nanoparticle Research, 15, 1-11.
• Gopinath, K., Devi, N. P., Govindarajan, M., Bhakyaraj, K., Kumaraguru, S., Arumugam, A., Alharbi, N. S., Kadaikunnan, S., Benelli, G. (2017). One-Pot green synthesis of silver nanoparticles using the orchid leaf extracts of Anoectochilus elatus: Growth inhibition activity on seven microbial pathogens. Journal of Cluster Science, 28, 1541-1550.
• Gurunathan, S., Han, J. W., Kwon, D. N., Kim, J. H. (2014). Enhanced antibacterial and anti-biofilm activities of silver nanoparticles against Gram-negative and Gram-positive bacteria. Nanoscale Research Letters, 9, 1-17.
• Gurunathan, S., Raman, J., Malek, N. A., John, P. A., Vikineswary, S. (2013). Green synthesis of silver nanoparticles using Ganoderma neo-japonicum Imazeki: a potential cytotoxic agent against breast cancer cells. International Journal of Nanomedicine, 8, 4399-4413.
• Khan, A. U., Malik, N., Khan, M., Cho, M., Khan, M. M. M. (2018). Fungi-assisted silver nanoparticle synthesis and their applications. Bioprocess and Biosystem Engineering, 41, 1-20.
• Logeswari, P., Silambarasan, S., Abraham, J. (2015). Synthesis of silver nanoparticles using plants extract and analysis of their antimicrobial property. Journal of Saudi Chemical Society, 19, 311-317.
• Lopes, C. R. B., Courrol, L. C. (2018). Green synthesis of silver nanoparticles with extract of Mimusops coriacea and light. Journal of Luminescence, 199, 183-187.
• Mohammed, A. E., Al-Qahtani, A., Al-Mutairi, A., Al-Shamri, B., Aabed, K. (2018). Antibacterial and cytotoxic potential of biosynthesized silver nanoparticles by some plant extracts. Nanomaterials-Basel. 8, 1-15.
• Onitsuka, S., Hamada, T., Okamura, H. (2019). Preparation of antimicrobial gold and silver nanoparticles from tea leaf extracts. Colloids and Surfaces B, 173, 242-248.
• Pirtarighat, S., Ghannadnia, M., Baghshahi, S. (2019). Green synthesis of silver nanoparticles using the plant extract of Salvia spinosa grown in vitro and their antibacterial activity assessment. Journal of Nanostructure in Chemistry, 9, 1-9.
• Raj, S., Mali, S. C., Trivedi, R. (2018). Green synthesis and characterization of silver nanoparticles using Enicostemma axillare (Lam.) leaf extract. Biochemical and Biophysical Reserach Communications, 503, 2814-2819.
• Rajakumar, G., Gomathi, T., Thiruvengadam, M., Rajeswari, V. D., Kalpana, V. N., Chung, I. M. (2017). Evaluation of anti-cholinesterase, antibacterial and cytotoxic activities of green synthesized silver nanoparticles using from Millettia pinnata flower extract. Microbial Pathogenesis, 103, 123-128.
• Ramanathan, S., Gopinath, S. C. B., Anbu, P., Lakshmipriya, T., Kasim, F. H., Lee, C. G. (2018). Eco-friendly synthesis of Solanum trilobatum extract-capped silver nanoparticles is compatible with good antimicrobial activities. Journal of Molecular Structure, 1160, 80-91.
• Sharma, P., Pant, S., Rai, S., Yadav, R. B., Dave, V. (2018). Green Synthesis of silver nanoparticle capped with Allium cepa and their catalytic reduction of textile dyes: An ecofriendly approach. Journal of Polymers and The Environonment, 26, 1795-1803.
• Singh, P., Kim, Y. J., Singh, H., Wang, C., Hwang, K. H., Farh, M. E. A., Yang, D. C. (2015). Biosynthesis, characterization and antimicrobial applications of silver nanoparticles. International Journal of Nanomedicine, 10, 2567-2577.
• Veerasamy, R., Xin, T. Z., Gunasagaran, S., Xiang, T. F. W., Yang, E. F. C., Jeyakumar, N., Dhanaraj, S. A. (2011). Biosynthesis of silver nanoparticles using mangosteen leaf extract and evaluation of their antimicrobial activities. Journal of Saudi Chemical Society, 15, 113-120.
• Velammal, S. P., Devi, T. A., Amaladhas, T. P. (2016). Antioxidant, antimicrobial and cytotoxic activities of silver and gold nanoparticles synthesized using Plumbago zeylanica bark. Journal of Nanostructure in Chemistry, 6, 247-260.
• Venugopal, K., Rather, H. A., Rajagopal, K., Shanthi, M. P., Sheriff, K., Illiyas, M., Rather, R. A., Manikandan, E., Uvarajan, S., Bhaskar, M., Maaza, M. (2017). Synthesis of silver nanoparticles (AgNPs) for anticancer activities (MCF 7 breast and A549 lung cell lines) of the crude extract of Syzygium aromaticum. Journal of Photochemistry and Photobiology B: Biology, 167, 282-289.
• Wang, M. M., Zhang, W. J., Zheng, X. S., Zhu, P. Z. (2017). Antibacterial and catalytic activities of biosynthesized silver nanoparticles prepared by using an aqueous extract of green coffee bean as a reducing agent. Rsc Advances, 7, 12144-12149.