MEME KANSERİNDE MİTOMİR’LERİN TANIMLANMASI: İN VİTRO ÇALIŞMA İLE ROLLERİNİN GÖSTERİLMESİ

Öz

Amaç: Kadınlarda meme kanseri %5 oranında genetik yatkınlıkla ilişkilidir. Mitokondri, metabolizma, hücre ölümü ve inflamasyon gibi önemli hücresel olaylarda rol oynar. Son çalışmalar, olgun miRNA’ların yanı sıra pre-miRNA’ların mitokondride bulunduğunu göstermişlerdir. Bu çalışmada meme kanseri hücrelerinde mitomiR’lerin varlığını ortaya koymayı amaçladık. Gereç ve Yöntem: MACS yöntemi kullanılarak mitokondriyal fraksiyonlar hazırlandı ve küçük RNA dizilimi yapıldı. Bulgular: Mitokondriyal genoma hizalanmış bilinen ve yeni mitomiR dizilerini belirledik. Sonuç: Yeni mitomiR’lerin tanımlanması literatüre önemli katkılar sağlayabilir ve mitomiR biyogenezi moleküler mekanizmalar ile aydınlatılabilir.

Anahtar Kelimeler

• meme kanseri
• mitomiR
• MACS
• küçük RNA dizileme

EXPLORING MITOMIRS IN BREAST CANCER: AN IN-VITRO STUDY OF THEIR EMERGING ROLES

Öz

Objective: Breast cancer is associated with a 5% genetic predisposition in women. Mitochondria play a role in important cellular events such as metabolism, cell death, and inflammation. Recent studies have highlighted precursor micro-RNA (pre-miRNA) to be located in mitochondria as well as mature miRNA. This study aims to reveal the occurrence of mitochondrial miRNA (mitomiR) in breast cancer cells. Materials and Methods: The study has prepared the mitochondrial fractions using the magnetic-activated cell sorting (MACS) method and performed small RNA (sRNA) sequencing. Results: The study has identified known and novel mitomiR sequences aligned to the mitochondrial genome. Conclusion: Identifying new mitomiRs can provide significant contributions and illuminate the molecular mechanism underlying mitomiR biogenesis.

Anahtar Kelimeler

• mitomiRs
• MACS
• breast cancer
• small RNA sequencing

Kaynakça

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