EKSTRASELLÜLER MATRİKS BİLEŞENLERİNDEN TİP 1 VE TİP 3 KOLLAJENİN KARACİĞER KANSERİ İNSAN HÜCRE HATTI MODELİNDE HÜCRE PROLİFERASYONUNA VE ALFA FETO PROTEİN EKSPRESYONU ÜZERİNE ETKİLERİ

Öz

Amaç: Kanser araştırmalarında, hücrelerin farklı ortamlardaki davranışlarını gösteren çalışmalar yeni tedavi yöntemlerinin geliştirilmesi için büyük önem taşımaktadır. Mikroçevre hem normal doku hem de kanserli doku için çok önemlidir. Ekstraselüler matriks (ECM) ana bileşenleri kollajenlerdir. Hepatosellüler karsinom hücre hattı olan HepG2 hücrelerinde Tip 1 ve Tip 3 kollajenin hücre proliferasyonu ve karsinojenite göstergesi olan alfa-feto protein (AFP) ekspresyonu üzerine etkisi incelenmiştir. Gereç ve Yöntem: Proliferasyon analizi mikroskobik inceleme ve hücre canlılığı değerlendirme kiti (Cell Counting Kit-8, CCK-8) ile yapıldı. AFP ölçümleri immünolojik boyama yöntemleri kullanıla rak konfokal mikroskop ile yapıldı. Bulgular: Sonuçlar, Tip 3 kollajen ortamında büyüyen HepG2 hücrelerinin canlılık oranının kontrol grubuna göre istatistiksel olarak daha yüksek olduğunu gösterdi (p<0,0001). AFP ekspre syonu 24. saatte kontrol grubuna kıyasla Tip 3 kollajen varlığında anlamlı olarak artmış (p<0,05), diğer kültür zamanlarında ise AFP ekspresyonu Tip 1 kollajen kültür ortamında daha fazla görülmüştür. Sonuç: Her iki tip kollajende kültüre edilen HepG2 hücrelerinin morfolojik olarak benzer bir yapıya sahip olduğu, Tip I kollajen ve Tip 3 kollajenin kanser hücreleri üzerinde proliferasyonu artırıcı etkiye sahip olduğu belirlenmiştir. Karaciğer kanserinin göstergelerinden biri olan AFP, Tip 1 kollajen ve Tip 3 kollajen içeren kültür ortamlarında yüksek bulunmuş, bunun da kollajen tiplerinin AFP ekspresyonu üzerinde kötü prognoza doğru bir eğilim gösterdiğini düşündürmektedir.

Anahtar Kelimeler

• Alfa fetoprotein
• Ekstrasellüler matriks
• He patosellüler karsinoma
• Tip 1 kollajen
• Tip 3 kollajen

EFFECTS OF COLLAGEN TYPE 1 AND 3 ON CELL PROLIFERATION AND ALPHA FETO PROTEIN EXPRESSION IN A HUMAN CELL LINE MODEL OF LIVER CANCER

Öz

Objective: In cancer research, studies showing the behaviour of cells in different environments is important for developing new treatment methods. The microenvironment is essential for both regular and cancerous tissues. The main component of the extracellular matrix (ECM) is collagen. In HepG2 cells, a hepatocellular carcinoma cell line, the effects of Type 1 and Type 3 collagen on cell proliferation and the expression of alpha-feto protein (AFP), which is an indicator of carcinogenicity, was examined. Material and Method: HepG2 cells were grown in Type 1 or Type 3 covered surfaces, whereas no treatment was applied to the control group. Proliferation analysis was performed via microscopic examination and cell viability assessmet kit (Cell Counting Kit-8, CCK-8). AFP was measured using a confocal microscope using immunological staining. Result: The results showed that the viability rate of HepG2 cells growing in Type 3 collagen medium was statistically higher than in the control group (p<0.0001). AFP expression increased significantly in the presence of Type 3 collagen compared with the control group at the 24th hour (p<0.05), and at other culture times, AFP expression was seen more in Type 1 collagen culture medium. Conclusion: HepG2 cells cultured in both types of collagen have a morphologically similar structure, and Type 1 and Type 3 collagen have a proliferation-enhancing effect on cancer cells. AFP, an indicator of liver cancer, is high in culture media containing Type 1 and Type 3 collagen and this finding is considered as the tendency of bad prognosis of collagen types on AFP expression.

Anahtar Kelimeler

• Alpha-fetoprotein
• Extracellular matrix
• Hepatocellu lar carcinoma
• Type 1 collagen
• Type 3 collagen

Kaynakça

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