TAURIN KIRIK İYİLEŞMESİNİ ETKİLER Mİ? DENEYSEL BİR ÇALIŞMA

Öz

DOI: 10.26650/IUITFD.394531

Amaç: Yapısal olmayan bir amino asit olan antioksidan etkili taurinin kırık iyileşmesi üzerine etkilerinin araştırılması

Gereç ve Yöntem: Kontrol grubunda dokuz, taurin grubunda yedi tane Yeni Zelanda tipi tavşana açık tibial osteotomi uygulandı. Radyolojik kırık iyileşmesi, 21. günde çekilen direk grafi ve bilgisayarlı tomografi (BT) kesitleri üzerinde Goldberg skoru ile değerlendirildi. Histoloik olarak iyileşme Huo skoruna göre derecelendirildi. Sıfırıncı, yedinci, 14. ve 21. günlerde serumda malondialdehit (MDA) ve alkalen fosfataz (ALP) düzeyleri ölçüldü.  

Bulgular: Direk grafi ve BT kesitlerine göre kırık iyileşmesinin ortanca evresi taurin grubunda radyolojik kaynamayken kontrol grubunda olası kaynama olarak bulundu. Histolojik olarak, kallus taurin grubunda esas olarak kemik dokusundan oluşurken (ortanca Huo skoru 8), kontrol grubunda baskın doku kıkırdaktı (ortanca Huo skoru 6). MDA düzeyindeki değişimler oksidatif stresin kırık iyileşmesinin enflamatuvar fazında en yüksek olduğunu göstermekteydi. Her iki grupta da ALP düzeyleri önce azalmış, 21. Günde yeni kemik oluşumuyla yeniden yükselmiş olarak bulundu.

Sonuç:  Bu çalışmanın sonuçları deneysel osteotomi modelinde taurinin erken evre kırık iyileşmesi üzerinde olumlu etkileri olduğunu desteklemektedir. Bu etki tarunin antioksidan özelliği ile ilişkilendirilebilir.  

Anahtar Kelimeler

• Taurin,kırık iyileşmesi,oksidatif stres,antioksidan,malondialdehit (MDA)

DOES TAURINE IMPROVE FRACTURE HEALING? AN EXPERIMENTAL STUDY

Öz

DOI: 10.26650/IUITFD.394531

Objective: To evaluate the influence of taurine, a non-structural amino acid with antioxidant effects, on fracture healing.

Materials and Methods: Open tibial osteotomy was performed on nine New Zealand rabbits in the control group and seven in the taurine group. Radiologic fracture healing was assesed using the Goldberg score on x-rays and computed tomography (CT) sections on the 21st day. Healing was graded histologically using the Huo score. Serum malondialdehyde (MDA) and alkaline phosphatase (ALP) levels were measured, at days zero, seven, 14 and 21.

Results: Upon completion of the study, in the taurine group, the median stage of healing was recorded as “radiologic union”, whereas it was “probable union” in the control group with respect to x-ray and CT sections. In the taurine group, callus was mainly composed of osteoid tissue (median Huo score 8); in the control group the predominant tissue was chondroid (median Huo score 6). Changes in MDA levels revealed that oxidative stress was greatest in the inflammatory phase. In both groups, the ALP levels first decreased, and then increased ,with new bone formation on the 21st day.

Conclusion: Taurine improved early bone healing in an experimental animal osteotomy model. This influence might be related to its antioxidant properties.

Anahtar Kelimeler

• Taurine,fracture healing,oxidative stress,antioxidants,malondialdehyde (MDA)

Kaynakça

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