KARACİĞER HEMOSTAZINDA YENİ BİR BİTKİSEL İLAÇ: DENEYSEL BİR HAYVAN ÇALIŞMASI

Yıl 2019, , 156 - 161, 16.07.2019

Enver Kunduz , Zeyneb Afra Dinçer Büşra Şeker , Hatice Kübra Saylak Okan Demir Hüseyin Akbulut Erhan Ayşan

https://doi.org/10.26650/IUITFD.2019.0014

Öz

Amaç: Kanama, cerrahi işlemlerin ana komplikasyonlarından biridir. Karaciğer hasarı sonrası ciddi kanamalar görülebilir. Literatürde kompresyon, Pringle manevrası ve diğer hemostatik ajanlar gibi karaciğer kanamasını durdurmak için çeşitli yöntemler tanımlanmıştır. Bu çalışmada, literatürde anti-enflamatuar, antioksidan, antimikrobiyal, analjezik ve antikoagülan etkileri gösterilmiş ajanların kullanımının, karaciğer yaralanmalarında hemostatik etkinlikleri değerlendirildi. Gereç ve Yöntem: Bu çalışmada 28 adet Wistar albino dişi / erkek sıçan kullanıldı. Hayvanlar 4 gruba ayrıldı. Karaciğer hasarı modeli oluşturulduktan sonra, karaciğere çeşitli bitkisel karışımlar uygulandı. Hematokrit ve trombosit sayıları ölçüldü. Ayrıca karaciğer parankimindeki histopatolojik değişiklikler için, sıçanlar sakrifiye edilerek karaciğer rezeke edildi. Bulgular: Kanama miktarı (p:0,001) ve preoperatif ve postoperatif hematokrit değişiklikleri (p:0,009) gruplar arasında istatistiksel olarak anlamlıydı. Bağ dokusu büyümesinde (p:0,065) ve nekrozda anlamlı bir fark yoktu (p:0,062). Karyorreksis olmadan iltihaplanmada anlamlı fark vardı (p:0,003). Tartışma: Histopatolojik incelemeler aktif ajan kullanan grupların daha fazla bağ dokusu ve karyorreksi, kanamayı durdurmaya yardım edebilmektedir.

Anahtar Kelimeler

Karaciğer kanaması, Bitkisel ilaç, hemostatik ajan

A NOVEL HERBAL EXTRACT MEDICINE FOR LIVER HEMOSTASIS: AN EXPERIMENTAL ANIMAL STUDY

Öz

Objective: Bleeding is one of the major complications of surgical procedures. Severe bleeding can be seen after liver injury. Various methods have been described in the literature for stopping liver bleeding such as compression, Pringle maneuver, and other hemostatic agents have been used. In this study, the use of anti-inflammatory, antioxidant, antimicrobial, analgesic, and anticoagulant agents described in the literature were evaluated for hemostatic efficacy following liver injuries. Materials and Methods: In this study 28 Wistar albino female / male rats from out-bred production were used. Animals were divided in 4 groups. After liver damage model creation, various herbal substances were applied to the liver. Hematocrit and platelet counts were measured. In addition, for histopathologic changes in the liver parenchyma, the rats were sacrificed, and the liver was resected. Results: The amount of bleeding (p:0.001) and the preoperative and postoperative hematocrit changes (p:0.009) were statistically significant between the groups. There was no significant difference in connective tissue growth (p:0.065) and necrosis (p:0.062). There were significant differences in inflammation and without karyorrhexis (p:0.003). Discussion: Histopathological examinations showed that the groups using active agents had more connective tissue and karyorrhexis. Therefore these tissues could help to stop bleeding.

Anahtar Kelimeler

Liver bleeding, Herbal medicine, hemostatic agent

Kaynakça

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