SERVİKOVAJİNAL MİKROFLORADAN İZOLE EDİLEN LACTOBACİLLUS PLANTARUM L4 SUŞUNUN HELA KANSER HÜCRE HATTI ÜZERİNDEKİ ANTİPROLİFERATİF ETKİSİ

Öz

Amaç: Lactobacillus spp. bakterilerinin çeşitli kanser hücrelerinin proliferasyonunu inhibe ettiği gösterilmiştir. Ancak, vajinal Lactobacillus’ların rahim ağzı kanseri hücreleri üzerindeki etkileri nadiren bildirilmiştir. Bu çalışmada Türkiye’de yaşayan sağlıklı kadınların servikovajinal florasından izole edilen L. plantarum L4 suşunun çeşitli probiyotik karakterleri ve HeLa kanser hücre hattı üzerindeki antiproliferatif etkisi araştırılmıştır. Gereç ve Yöntemler: Türün moleküler tanımlaması 16S rDNA analizi ile gerçekleştirilmiştir. L4 suşunun probiyotik özellikleri geleneksel yöntemlerle belirlenmiştir. L4 suşunun immun modilatör etkisini belirlemede insan Interleukin-10 (IL-10) ve Tümör Nekroz Factor-alfa (TNF-α) ELISA kitleri kullanılmıştır. HeLA hücre hattı üzerindeki antiproliferatif etkisi XTT kiti kullanılarak gerçekleştirilmiştir. Bulgular: L4 suşu güçlü probiyotik özellikler sergilemiştir. L4 suşunun, TNF-α üretimini azaltarak HeLa hücreleri üzerinde anti-inflamatuar bir etki gösterdi ve IL-10 üretiminin artırılmasını sağladı. L. plantarum L4 suşunun HeLa hücreleri üzerinde en büyük antiproliferatif etkinin metabolitin 0,0006 gr/ml’lik en yüksek dozunda %90-95 düzeyinde ölüm oranı gözlemlenirken canlı hücre sayısının %5-10 arasında olduğu görülmüştür. İnsan göbek ven endotel hücreleri (HUVEC) üzerinde herhangi bir antikanser etkisi olmamıştır. Sonuç: Güçlü probiyotik özelliklere sahip L. plantarum L4 suşu, immunmodilatör etkisi ve çok küçük dozlarda bile yüksek bir antiproliferatif etki göstermesi sebebiyle HPV kanseri için umut verici bir tedavi adayı olarak kabul edilebilir.

Anahtar Kelimeler

• Lactobacillus plantarum
• antiproliferatif etki
• HeLa
• IL-10
• TNF-α

ANTIPROLIFERATIVE EFFECT OF LACTOBACILLUS PLANTARUM L4 STRAIN ISOLATED FROM CERVICOVAGINAL MICROFLORA ON HELA CANCER CELL LINE

Öz

Objective: Lactobacillus has been shown to inhibit proliferation of various cancer cells, but the effects of vaginal Lactobacillus on cervical cancer cells have rarely been reported. The goal of this investigation was to assess the anti-proliferative effect on cancer cell line HeLa (Human Cervical Carcinoma Cell) and potential probiotic properties of Lactobacillus plantarum L4 isolated from cervicovaginal flora of healthy women in Turkey. Materials and Methods: Molecular identification of the species was performed by 16S rDNA analysis. Probiotic properties of the L4 strain were investigated by conventional methods. Human Interleukin-10 (IL-10) and Tumor Necrosis Factor- alpha (TNF-alpha) ELISA kits were used in the evelation of the immune modulator effect of the L4 strain. The antiproliferative effect of the L4 strain on the HeLA cell line was performed using the XTT kit. Results: L. plantarum L4 strain exhibited strong probiotic properties. The L4 strain showed an anti-inflammatory effect on HeLa by reducing the production of TNF-α and increased IL-10 production. The greatest antiproliferative effect of L. plantarum L4 strain on HeLa cells was observed at the highest dose of the metabolite 0.0006 gr/ml with a death rate of 90-95% while the number of living cells was found to be between 5-10%. The strain showed no anticancer effect on human umbilical vein endothelial cells (HUVEC). Conclusion: L. plantarum L4 strain, with strong probiotic properties, can be considered a promising treatment candidate for HPV cancer due to its immunomodulatory effect and high antiproliferative effect, even in very small doses.

Anahtar Kelimeler

• Lactobacillus plantarum
• Antiproliferative effect
• IL-10
• HeLa
• TNF-α

Kaynakça

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