ROMATOİD ARTRİT’TE FC GAMA RESEPTÖR IIIA V/158/F GEN POLİMORFİZMİNİN SIKLIĞININ ARAŞTIRILMASI VE HASTA KLİNİK VE LABORATUAR BULGULARIYLA KARŞILAŞTIRILMASI

Yıl 2018, Cilt: 81 Sayı: 4, 139 - 144, 01.12.2018

Ali Murat Sedef Suzan Dikci Eren Erken

Öz

DOI: 10.26650/IUITFD.426162

Amaç: Romatoid Artrit (RA), nedeni
bilinmeyen, esas olarak periferik eklemleri progresif olarak tutan kronik
multisistemik bir hastalıktır. Hümoral ve hücresel immün yanıtları arasında bir
bağ olan, IgG‘ nin Fc reseptörleri otoimün hastalıkların etyoloji ve
patogenezinde oldukça ilgi çekmektedir. Geniş genetik varyasyon sergileyen bu
bölge Romatoid artrit de dahil olmak üzere çeşitli kronik inflamatuvar
hastalıklara yatkınlık ile ilişkilidir. Bu çalışmasında Romatoid Artrit‘ te
FcγRIIIA V158F gen polimorfizminin sıklığının araştırılması ve hasta klinik ve
laboratuar bulgularıyla karşılastırılması amaçlanmıştır.

Gereç ve Yöntem: Çukurova Üniversitesi Tıp
Fakültesi Romatoloji Bilim Dalı polikliniğine Nisan 2010-Haziran 2011 tarihleri
arasında başvuran ve Amerikan Romatizma Derneği‘ nin tanı kriterlerine göre RA
tanısı almış 105 RA‘lı hasta ve 110 sağlıklı kontrol çalışmaya dahil edildi.
Hasta ve kontrollerden öyküsünde diyabet, tiroid fonksiyon bozuklukları,
geçirilmiş veya mevcut kalp hastalıkları, malignite, nörolojik hastalıklar ile
kronik inflamatvuar hastalıklardan bir ya da daha fazlası olanlar çalışma dışı
bırakıldı. RA‘ lı hastaların hastalıkla ilgili semptomları, sistemik hastalık
varlığı, ilaç kullanımı, hastalık süreleri ve aile öyküsü sorgulandı. Tüm
olgulara genel fizik muayene ve romatolojik muayeneleri yapıldı ve rutin
laboratuvar tetkikleri ile CRP, RF, ESR, ANA, Anti-DNA düzeyleri istendi. Hasta
grubu ARA‘nın fonksiyonel sınıflandırması kullanılarak evrelere ayrıldı. Alınan
kan örneklerinde real time PCR yöntemi ile FcγRIIIA V158F gen polimorfizmleri
araştırıldı.

Bulgular: Hasta ve kontrol grubunun Fc γ
RIIIA gen polimorfizm dağılımları arasında anlamlı fark saptanmadı (p=0,106).
Hastaların tanı aldıkları yaş dağılımları ile gen polimorfizmi arasında anlamlı
fark saptanmadı (p=0,919). Hastaların cinsiyet dağılımlarına göre de gen
polimorfizmi açısından anlamlı fark saptanmadı (p=0,552). Hastalığın klinik
bulgularından olan akciğer ve göz tutulumları, romatoid nodül varlığı ile gen
polimorfizmi arasında ilişki saptanmadı. RF ve ANA sonuçları ile gen
polimorfizmi arasında da anlamlı fark saptanmadı (p=0,625, p=0,716). Hastaların
anti-CCP sonuçları ile gen polimorfizmi arasında anlamlı fark saptanmadı
(p=0,136).

Sonuç: Çalışmamızda RA patogenezi ile Fc γ
RIIIA 158 gen polimorfizmi arasında anlamlı bir ilişki saptanmamıştır. 

Anahtar Kelimeler

FcγRIIIA gen polimorfizmi, romatoid artrit

INVESTIGATION OF THE FREQUENCY OF FC GAMMA RECEPTOR IIIA V/158/F GENE POLYMORPHISM AND COMPARISON OF CLINICAL AND LABORATORY FINDINGS IN RHEUMATOID ARTHRITIS (RA)

Öz

DOI: 10.26650/IUITFD.426162

Objective: Rheumatoid Arthritis (RA) is a
chronic multisystem disease with unknown etiology that progressively affects
peripheral joints. Receptors, which serve as links between humoral and cellular
immune responses, recognize the Fc region of immunoglobulin G (FcR) and have
become the focus of many research studies concerning the etiology and
pathogenesis of autoimmune diseases. This region displays extensive genetic
variation, which has been associated with susceptibility to various chronic
inflammatory disorders including RA. This study aimed to investigate the
frequency of the FcγRIIIA V158F genetic polymorphism and compare clinical and
laboratory findings in RA.

Materials and Methods: Between April 2010
and June 2011, 105 patients, who had been diagnosed with RA according to the
American Rheumatology Association (ARA) diagnostic criteria, were admitted to
the Cukurova University Department of Rheumatology outpatient clinic and 110
healthy controls were included in this study. Patient groups were divided into
stages using the ARA functional classification. The FcγRIIIA V158F gene polymorphism
of patients was investigated from blood samples using the real-time Polymerase
Chain Reaction (PCR) method.

Results: There was no significant
difference in the distribution of the FcγRIIIA polymorphism between patients
and controls (p=.106). There were no significant differences between the
distribution of age at diagnosis of patients with the gene polymorphism
(p=.919) or in the gene polymorphism (p=.552). No association was found between
the gene polymorphism and clinical signs of disease such as eye involvement and
the presence of rheumatoid nodules. There was also no significant association
between the gene polymorphism with rheumatoid factor, anticyclic citrullinated
peptide, and antinuclear antibodies (p=.625, p=.136, p=.716, respectively).

Conclusion: In our study, no significant
relationship was found between the FcγRIIIA V158F gene polymorphism and the
pathogenesis of RA.

Anahtar Kelimeler

FcγRIIIA V158F gene polymorphism, rheumatoid arthritis

Kaynakça

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