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RETİNOİK ASİT UYGULANAN SIÇANLARIN PLASENTALARINDA MMP-2 VE MMP-13 İMMÜNREAKTİVİTESİNİN İNCELENMESİ

Yıl 2010, Cilt: 73 Sayı: 2, 46 - 51, 10.11.2011

Öz

Amaç: Matriks metalloproteinazlar (MMP) hücrelerarası matriks yıkımında rol alarak implantasyonun gerçekleşmesine yardımcı olan enzimlerdir. Retinoik asit (RA); MMP üretimi üzerine etkisi olduğu düşünülen, trofoblast invazyonunda rolü olan bir moleküldür. Çalışmamızda retinoik asit uygulanan gebe sıçanların plasentalarında MMP-2 ve MMP-13 immünreaktivitesinin incelenmesi amaçlandı. Gereç ve Yöntem: Çalışmada kontrol (8 adet) ve deney (8 adet) gruplarında toplam 16 adet dişi Spraque Dawley türü sıçan kullanıldı. Sıçanlar gebe bırakılarak deney grubundakilere gastrulasyon döneminde 3 gün süre ile 40mg/kg/gün 13-cis retinoik asit oral yolla uygulandı. Gebeliğin orta döneminde (10-14 gün) plasentalardan alınan kesitler immünhistokimyasal olarak Streptavidin- Biyotin yöntemi kullanılarak rabbit poliklonal MMP-13 antikoru ve mouse monoklonal MMP-2 antikoru ile işaretlendi. Bulgular: Kontrol grubuna ait plasentalarda MMP-2 immünreaktivitesi trofoblastik dev hücrelerde kuvvetli pozitif (+++), koriyo-amniyotik zarda ise orta derecede pozitif (++) bulundu. RA uygulanan grupta trofoblastik dev hücrelerde MMP-2 immünreaktivitesi saptanmadı, buna karşın koriyo-amniotik zarda kuvvetli pozitiflik mevcuttu. MMP-13 immünreaktivitesi ise kontrol grubu dev hücrelerinde ve korio-amniyotik zarda orta derecede (++) pozitifti. RA grubunda dev hücrelerde değişiklik görülmezken koriyo-amniyotik zarda kuvvetli pozitif (+++) işaretlenme mevcuttu. Sonuç: Eksojen olarak uygulanan retinoik asitin trofoblastik dev hücrelerde ve desiduada MMP-2 sentezini baskılayarak implantasyonu olumsuz etkileyebileceği ve koriyo-amniyotik zarda ise MMP-2 ve MMP-13‟ü arttırmak suretiyle membran stabilizasyonunu bozabileceği, bunlara bağlı olarak hayatta kalımı engelleyebileceği kanaatine varıldı.

Kaynakça

  • Alexander CM, Hansell EJ, Behrendtsen O, Flannery ML, Kishnani N, Hawkes SP, Werb Z. Expression and function of matriks metalloproteinases and their inhibitors at the maternal-embryonic boundary during mouse embryo implantation. Development 1996; 122: 1723-1736.
  • Braunhut SJ, Moses MA. Retinoids modulate endothelial cell production of matrix-degrading proteases and tissue inhibitors of metalloproteinases (TIMP). The Journal of Biological Chemistry 1994; 269 : 13472-13479.
  • Das SK, Yano S, Wang J, Edwards DR, Nagase H, Dey SK. Expression of Matrix Metalloproteinases and Tissue Inhibitors of Metalloproteinases in the Mouse Uterus During the Peri-Implantation Period. Developmental Genetics 1997; 21:44-54.
  • El-Hashash AH, Esbrit P, Kimber SJ. PTHrP promotes murine secondary trophoblast giant cell differentiation through induction of endocycle, upregulation of giant- cell-promoting transcription factors and suppression of other trophoblast cell types. Differentiation 2005; 73: 154–174.
  • Fortunato SJ, Menon R. Screening of Novel Matrix Metalloproteinases (MMPs) in Human Fetal Membranes. Journal of Assisted Reproduction and Genetics 2002; 19:483-486.
  • Goldman S, Weiss A, Eyali V, Shalev E. Differential activity of gelatinases (matrix metalloproteinases 2 and 9) in the fetal membranes and decidua, associated with labour. Molecular Human Reproduction 2003; 9: 367- 373.
  • Hasan S, Nakajima M. Retinoic acid synergizes with cyclic AMP to enhance MMP-2 basal promoter activity. Biochemical and Biophysical Research Communications 1999; 258: 663-667.
  • Hendrix MJC, Wood WR, Seftor EA, Lotan D, Nakajima M, Misiorowski RL, SeftorREB, Stetler-Stevenson WG, Bevacqua SJ, Liotta LA, Sobel ME, Lotan R. Retinoic acid inhibition of human melanoma cell invasion through a reconstituted basement membrane and its relation to decreases in the expression of proteolytic enzymes and motility factor receptor. Cancer Res 1990; 50:4121-4130.
  • Huang F. Effects of retinoic acid on morula-stage embryo development in mice. Chang Gung Med J 2008;31:44-51.
  • Kanai-Azuma M, Kanai Y, Matsuda H, Kurohmaru M, Tachi C, Yazaki K, Hayashi Y. Nerve growth factor 50
  • Teesalu T, Masson R, Basset P, Blasi F, Talarıco D. Expression of matriks metalloproteinases during murine chorioallantoic Dynamics 1999; 214: 248-258. maturation. Developmental
  • Tremblay GB, Kunath T, Bergeron D, Lapointe L, Champigny C, Bader JA, Rossant J, Giguere V. Diethylstilbestrol differentiation as a ligand of orphan nuclear receptor ERR beta. Genes Dev 2001; 15: 833–838. stem cell
  • Uchide K, Ueno H, Inoue M, Sakaı A, Fujimoto N, Okada Y. Matrix metalloproteinase-9 and tensile strength of fetal membranes in uncomplicated labor. Obstet Gynecol 2000; 95: 851-855.
  • Ulug U, Goldman S, Ben-Shlomo I, Shalev E. Matrix metalloproteinase (MMP)- 2 and MMP-9 and their inhibitor, TIMP-1, in human term decidua and fetal membranes: the effect of prostaglandin F2α and indomethacin. Molecular Human Reproduction 2001; 7: 1187-1193.
  • Walter I, Schönkypl S. Extracellular matrix components and matrix degrading enzymes in the feline placenta during gestation. Placenta 2006; 27: 291-306.
  • Weiss A, Goldman S, Shlomo IB, Eyali V, Leibovitz S, Shalev E. Mechanisms of matrix metalloproteinase-9 and matrix metalloproteinase-2 inhibition by N-acetylcysteine in the human term decidua and fetal membranes. Am J Obstet Gynecol 2003; 189:1758-1763.
  • Winterhager E, Reuss B, Hellmann P, Spray DC, Gruemmer R. Gap junctionand tissue invasion: A comparison of tumorigenesis and pregnancy. Clin. Exp. Pharmacol. Physiol 1996; 23: 1058-1061.
  • Xu P, Alfaidy N, Challis JRG. Expression of matriks metalloproteinase (MMP)-2 and MMP-9 in human placenta and fetal membranes in relation to preterm and term labor. The Journal of Clinical Endocrinology & Metabolism 2002; 87: 1353-1361.
  • Xu P, Wang Y, Zhu S, Luo S, Piao Y, Zhuang K. Expression of matrix metalloproteinase-2, -9, and -14, Tissue Inhibitors of metalloproteinase-1, and Matrix proteins in human placenta during the first trimester. Biology of Reproduction 2000; 62: 988-994.
  • Yan J, Tanaka S, Oda M, Makino T, Ohgane J, Shiota K. Retinoic acid promotes differentiation of trophoblast stem cells to a giant cell fate. Developmental Biology 2001; 235: 422-432.
  • Expression and importance of matrix metalloproteinase 2
  • and 9 (MMP-2 and -9) in human trophoblast invasion.
  • Reproductive Biology and Endocrinology 2004; 2:59.
  • Stephanou A, Handwerger S. Retinoic acid and thyroid hormone regulate placental lactogen expression in human trophoblast cells. Endocrinology 1995; 136:933-938.
Yıl 2010, Cilt: 73 Sayı: 2, 46 - 51, 10.11.2011

Öz

Kaynakça

  • Alexander CM, Hansell EJ, Behrendtsen O, Flannery ML, Kishnani N, Hawkes SP, Werb Z. Expression and function of matriks metalloproteinases and their inhibitors at the maternal-embryonic boundary during mouse embryo implantation. Development 1996; 122: 1723-1736.
  • Braunhut SJ, Moses MA. Retinoids modulate endothelial cell production of matrix-degrading proteases and tissue inhibitors of metalloproteinases (TIMP). The Journal of Biological Chemistry 1994; 269 : 13472-13479.
  • Das SK, Yano S, Wang J, Edwards DR, Nagase H, Dey SK. Expression of Matrix Metalloproteinases and Tissue Inhibitors of Metalloproteinases in the Mouse Uterus During the Peri-Implantation Period. Developmental Genetics 1997; 21:44-54.
  • El-Hashash AH, Esbrit P, Kimber SJ. PTHrP promotes murine secondary trophoblast giant cell differentiation through induction of endocycle, upregulation of giant- cell-promoting transcription factors and suppression of other trophoblast cell types. Differentiation 2005; 73: 154–174.
  • Fortunato SJ, Menon R. Screening of Novel Matrix Metalloproteinases (MMPs) in Human Fetal Membranes. Journal of Assisted Reproduction and Genetics 2002; 19:483-486.
  • Goldman S, Weiss A, Eyali V, Shalev E. Differential activity of gelatinases (matrix metalloproteinases 2 and 9) in the fetal membranes and decidua, associated with labour. Molecular Human Reproduction 2003; 9: 367- 373.
  • Hasan S, Nakajima M. Retinoic acid synergizes with cyclic AMP to enhance MMP-2 basal promoter activity. Biochemical and Biophysical Research Communications 1999; 258: 663-667.
  • Hendrix MJC, Wood WR, Seftor EA, Lotan D, Nakajima M, Misiorowski RL, SeftorREB, Stetler-Stevenson WG, Bevacqua SJ, Liotta LA, Sobel ME, Lotan R. Retinoic acid inhibition of human melanoma cell invasion through a reconstituted basement membrane and its relation to decreases in the expression of proteolytic enzymes and motility factor receptor. Cancer Res 1990; 50:4121-4130.
  • Huang F. Effects of retinoic acid on morula-stage embryo development in mice. Chang Gung Med J 2008;31:44-51.
  • Kanai-Azuma M, Kanai Y, Matsuda H, Kurohmaru M, Tachi C, Yazaki K, Hayashi Y. Nerve growth factor 50
  • Teesalu T, Masson R, Basset P, Blasi F, Talarıco D. Expression of matriks metalloproteinases during murine chorioallantoic Dynamics 1999; 214: 248-258. maturation. Developmental
  • Tremblay GB, Kunath T, Bergeron D, Lapointe L, Champigny C, Bader JA, Rossant J, Giguere V. Diethylstilbestrol differentiation as a ligand of orphan nuclear receptor ERR beta. Genes Dev 2001; 15: 833–838. stem cell
  • Uchide K, Ueno H, Inoue M, Sakaı A, Fujimoto N, Okada Y. Matrix metalloproteinase-9 and tensile strength of fetal membranes in uncomplicated labor. Obstet Gynecol 2000; 95: 851-855.
  • Ulug U, Goldman S, Ben-Shlomo I, Shalev E. Matrix metalloproteinase (MMP)- 2 and MMP-9 and their inhibitor, TIMP-1, in human term decidua and fetal membranes: the effect of prostaglandin F2α and indomethacin. Molecular Human Reproduction 2001; 7: 1187-1193.
  • Walter I, Schönkypl S. Extracellular matrix components and matrix degrading enzymes in the feline placenta during gestation. Placenta 2006; 27: 291-306.
  • Weiss A, Goldman S, Shlomo IB, Eyali V, Leibovitz S, Shalev E. Mechanisms of matrix metalloproteinase-9 and matrix metalloproteinase-2 inhibition by N-acetylcysteine in the human term decidua and fetal membranes. Am J Obstet Gynecol 2003; 189:1758-1763.
  • Winterhager E, Reuss B, Hellmann P, Spray DC, Gruemmer R. Gap junctionand tissue invasion: A comparison of tumorigenesis and pregnancy. Clin. Exp. Pharmacol. Physiol 1996; 23: 1058-1061.
  • Xu P, Alfaidy N, Challis JRG. Expression of matriks metalloproteinase (MMP)-2 and MMP-9 in human placenta and fetal membranes in relation to preterm and term labor. The Journal of Clinical Endocrinology & Metabolism 2002; 87: 1353-1361.
  • Xu P, Wang Y, Zhu S, Luo S, Piao Y, Zhuang K. Expression of matrix metalloproteinase-2, -9, and -14, Tissue Inhibitors of metalloproteinase-1, and Matrix proteins in human placenta during the first trimester. Biology of Reproduction 2000; 62: 988-994.
  • Yan J, Tanaka S, Oda M, Makino T, Ohgane J, Shiota K. Retinoic acid promotes differentiation of trophoblast stem cells to a giant cell fate. Developmental Biology 2001; 235: 422-432.
  • Expression and importance of matrix metalloproteinase 2
  • and 9 (MMP-2 and -9) in human trophoblast invasion.
  • Reproductive Biology and Endocrinology 2004; 2:59.
  • Stephanou A, Handwerger S. Retinoic acid and thyroid hormone regulate placental lactogen expression in human trophoblast cells. Endocrinology 1995; 136:933-938.
Toplam 24 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Bölüm Klinik Araştırma
Yazarlar

Tuğba Kotil Bu kişi benim

Leyla Tapul Bu kişi benim

Yayımlanma Tarihi 10 Kasım 2011
Gönderilme Tarihi 10 Kasım 2011
Yayımlandığı Sayı Yıl 2010 Cilt: 73 Sayı: 2

Kaynak Göster

APA Kotil, T., & Tapul, L. (2011). RETİNOİK ASİT UYGULANAN SIÇANLARIN PLASENTALARINDA MMP-2 VE MMP-13 İMMÜNREAKTİVİTESİNİN İNCELENMESİ. Journal of Istanbul Faculty of Medicine, 73(2), 46-51.
AMA Kotil T, Tapul L. RETİNOİK ASİT UYGULANAN SIÇANLARIN PLASENTALARINDA MMP-2 VE MMP-13 İMMÜNREAKTİVİTESİNİN İNCELENMESİ. İst Tıp Fak Derg. Kasım 2011;73(2):46-51.
Chicago Kotil, Tuğba, ve Leyla Tapul. “RETİNOİK ASİT UYGULANAN SIÇANLARIN PLASENTALARINDA MMP-2 VE MMP-13 İMMÜNREAKTİVİTESİNİN İNCELENMESİ”. Journal of Istanbul Faculty of Medicine 73, sy. 2 (Kasım 2011): 46-51.
EndNote Kotil T, Tapul L (01 Kasım 2011) RETİNOİK ASİT UYGULANAN SIÇANLARIN PLASENTALARINDA MMP-2 VE MMP-13 İMMÜNREAKTİVİTESİNİN İNCELENMESİ. Journal of Istanbul Faculty of Medicine 73 2 46–51.
IEEE T. Kotil ve L. Tapul, “RETİNOİK ASİT UYGULANAN SIÇANLARIN PLASENTALARINDA MMP-2 VE MMP-13 İMMÜNREAKTİVİTESİNİN İNCELENMESİ”, İst Tıp Fak Derg, c. 73, sy. 2, ss. 46–51, 2011.
ISNAD Kotil, Tuğba - Tapul, Leyla. “RETİNOİK ASİT UYGULANAN SIÇANLARIN PLASENTALARINDA MMP-2 VE MMP-13 İMMÜNREAKTİVİTESİNİN İNCELENMESİ”. Journal of Istanbul Faculty of Medicine 73/2 (Kasım 2011), 46-51.
JAMA Kotil T, Tapul L. RETİNOİK ASİT UYGULANAN SIÇANLARIN PLASENTALARINDA MMP-2 VE MMP-13 İMMÜNREAKTİVİTESİNİN İNCELENMESİ. İst Tıp Fak Derg. 2011;73:46–51.
MLA Kotil, Tuğba ve Leyla Tapul. “RETİNOİK ASİT UYGULANAN SIÇANLARIN PLASENTALARINDA MMP-2 VE MMP-13 İMMÜNREAKTİVİTESİNİN İNCELENMESİ”. Journal of Istanbul Faculty of Medicine, c. 73, sy. 2, 2011, ss. 46-51.
Vancouver Kotil T, Tapul L. RETİNOİK ASİT UYGULANAN SIÇANLARIN PLASENTALARINDA MMP-2 VE MMP-13 İMMÜNREAKTİVİTESİNİN İNCELENMESİ. İst Tıp Fak Derg. 2011;73(2):46-51.

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