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SIÇANLARIN KARACİĞERLERİNDE D-GALAKTOZAMİNİN OKSİDATİF STRES VE APOPTOZ ÜZERİNE ETKİSİ

Yıl 2008, Cilt: 71 Sayı: 2, 29 - 32, 14.11.2011

Şule Seçkin Şeyda Alsancak Canan Başaran Küçükergin Makbule Aydın Şule Seçkin At All.

Öz

Amaç: Çalışmamızda, yüksek doz D-galaktozamin (D-GalN) uygulamasının sıçanlarda oksidatif stres ve apoptoz üzerine etkisini inceledik. Gereç ve yöntem: Sıçanlara intraperitonal olarak, tek dozda 1g/kg D-GalN uygulandı. Sıçanların karaciğer homojenatlarında malondialdehit(MDA) ve glutatyon (GSH) düzeyleri spektrofotometrik yöntemle ölçüldü. Karaciğer dokusunda TNF-alpha düzeylerinin ölçülmesi için ELİSA yöntemi uygulandı. Apoptozun bir göstergesi olan DNA fragmantasyonu kalitatif olarak agoroz jel elektroforezi ile gözlendi. Bulgular: Lipid peroksidasyonun bir göstergesi olan MDA düzeyleri 1g/kg başına D-GalN uygulamasından 24 saat sonra anlamlı olarak artış gösterirken GSH düzeylerinde azalma saptandı. Hepatik TNF-alpha düzeyleri ise kontrol gruba göre anlamlı olarak azaldı. DNA fragmantasyonu jel elektroforezi ile yapılan incelemede gözlenmedi. Sonuç: Yüksek doz GalN uygulamasının oksidatif stresi indüklediği ama apoptoz üzerinde etkili olmadığı ileri sürülebilir.

Anahtar Kelimeler

D-galaktozamin TNF-alfa oksidatif stres apoptoz

Kaynakça

  • Adamson GM, Billings RE. Tumor necrosis factor induced oxi- dative stress in isolated mouse hepatocytes. Arch Biochem Bi- ophys 1992; 294: 223-229.
  • Beutler E, Duron O, Kelly BM. Improved method for the deter- mination of blood glutathione. J Lab Clin Med 1963; 61: 882- 888.
  • Camussi G, Albano E, Tetta C., Bussolino F. The molecular ac- tion of tumor necrosis factor-a. Eur J Biochem 1991; 202: 3-14.
  • Cheng C C, Etoh J, Tanimura T, Egashira Y, Ohta T, Sanada H. Effects of dietary gluten on the hepatotoxic action of galactosa- mine and/or endotoxin in rats. Biosci Biotech Biochem 1996; 60: 439-443.
  • Endo Y, Kikuchi T, Nakamura M. Ornithine and histidine decar- boxylase activities in mice sensitised to endotoxin, interleukin-1 or tumour necrosis factor by D-galactosamine. Br J Pharmacol 1992; 107: 888-894.
  • Hu H, Chen R. Changes in free radicals, trace elements and ne- urophysiological function in rats with liver damage induced by D-galactosamine. Biol Tr Elem Res 1992; 34: 20-25.
  • Irita K, Okabe A, Koga A, Kurosawa K. Increased sinusoidal eff- lux of reduced and oxidized glutathione in rats with endoto- xin/D-galactosamine hepatitis. Circ Shock 1994; 42: 115-120.
  • Itokazu Y, Segawa Y, Inoue N, Omata T. D-Galactosamine-indu- ced mopuse hepatic apoptosis: possible involvement with tumor necrosis factor, but not with caspase-3 activity. Biol Pharm Bull 1999; 22 : 1127-1130.
  • Ledda-Columbano GM, Con P, Curto M.Induction of two diffe- rent modes of cell death, apoptosis and necrosis, in rat liver after a single dose of thioacetamide. Am J Pathol 1991; 139: 1099- 1109.
  • Louis H, Le Moine O, Peny M, Gulbis B. Hepatoprotective ro- le of interleukin 10 in galactosamine-lipopolysaccharide mouse liver injury. Gastroenterology 1997; 112: 935-942.
  • Lowry OH, Rosebrough NJ, Forry AL., Randall R J.Protein me- asurement with the folin phenol reagent. J Biol Chem 1951; 193: 265-275.
  • McMilla J, JollowDJ. Galactos-amine hepatotoxicity: Effect of galactosamine on glutathione resynthesis in rat primary hepa- tocyte cultures. Toxicol Appl Pharmacol 1992; 115: 234-240.
  • Miller S A, Dykes DD, Polesky HF. A simple salting out proce- dure for extracting DNA from human nucleated cells. Nucl Acids Res 1988; 16: 1215.
  • Molina PE, Abumrad NN. Differential effects of hemorrhage and LPS on tissue TNF-a, IL-1 and associate neuro-hormonal and opioid alterations. Life Sci 2000; 66: 399-409.
  • Muntane J, Montero JL., Marchal T, Perez-Seoane C, Lozano JM, Fraga E, Pintado CO. De la Mata M, Mino G. Effect of PGE1 on TNF-a status and hepatic D-galactosamine-induced apoptosis in rats. J Gastroenterol Hepatol 1998; 13, 197-207.
  • Nagakawa, J, Hitota K, Hishinuma I, Miyammoto K. Protective effect of E3330, a novel of quinone derivative, in galactosami- ne-induced hepatitis in rats. J Pharmacol Exp 1993. Ther 264; 496-500.
  • Ohta Y, Matsura T, Kitagawa A, Tokunaga K, Yamada K. Xant- hine oxidase-derived reactive oxygen species contribute to the development of D-galactosamine-induced liver injury in rats. Free Radic Res 2007; 41: 135-144.
  • Okhawa H, Ohishi N, Yagi K) Assay for lipid peroxides in ani- mal tissues by thiobarbituric acid. Anal Biochem 1979; 95: 351- 358.
  • Penning LC, Schipper RG, Vercammen D. Sensitization of TNF- induced apoptosis with polyamine synthesis inhibitors in diffe- rent human and murine tumour cell lines. Cytokine 1998; 10: 423-431.
  • Portoles MT, Catala M, Anton ARHepatic response to the oxi- dative stress induced by E. coli endotoxin: Glutathione as an in- dex of the acute phase during the endotoxic shock. Mol Cell Bi- ochem 1996; 159: 115-121.
  • Rikans LE, Decicco L.A, Hornbrook KR, Yamano T. Effect of age and carbon tetrachloride on cytokine concentrations in rat li- ver. Mech Ageing Develop.1999; 108: 173-182.
  • Santucci L, Fiorucci S, Chiorean M, Brunori P M) Interleukin 10 reduces lethality and hepatic injury induced by lipopolysaccha- ride in galactosamine - sensitized mice. Gastroenterology 1996; 111: 736-744.
  • Seçkin Ş , Alptekin N, Toker NK, Uysal M, Toker GA. Hepatic gamma-glutamyl cysteine synthetase and gamma-glutamyl transpeptidase activities in galactosamine-treated rats. Res Com- mun Mol Pathol Pharmacol 1995; 87: 237-240.
  • Shen W, Kamendulis LM, Ray SD, Corcoran GB. Acetaminop- hen--induced cytotoxicity in cultured mouse hepatocytes: corre- lation of nuclear Ca2+ accumulation and early DNA fragmenta- tion with cell death. Toxicol. Appl Pharmacol 1991; 111: 242- 254.
  • Stachlewitz RF, Seabra V, Bradford B, Bradham C A, Rusyn I, Germolec D, Thurman RG.) Glycine and uridine prevent D-ga- lactosamine hepatotoxicity in the rat: Role of Kupffer cells. He- patology 1999; 29: 737-745.
  • Sun F, Hamagawa E, Tsutsu, C, Sakaguchi N, Kakuta Y, Toku- maru S, Kojo S. Evaluation of oxidative stress during apoptosis and necrosis caused by D-galactosamine in rat liver. Biochem Pharmacol 2003; 65: 101-107.
  • Tsutsu, S, Hirasawa K, Takeda M, Itagaki S, Kawamura S, Ma- eda K. Apoptosis of murine hepatocytes induced by high doses of galactosamine. J Vet Med Sci 1997; 59: 785-790.
  • Tsutsui S, Hirasawa K, Takeda M, Itagaki S, Kawamura S, Ma- eda K, Mikami T, Doi K Galactosamine-induced apoptosis in the primary mouse hepatocyte cultures. Exp Toxic Pathol 1997; 49: 301-306.
  • Wang H, Xu D, Lv J, Ning H, Wie W. Melatonin attenuates li- popolysaccharide (LPS)-induced apoptotic liver damage in D- galactosamine-sensitized mice. Toxicology 2007; 237: 49-57.
  • Yoshikawa T, Furukawa Y, Murakami M, Takemura S, Kondo M. Effect of vitamin E on D-Galactosamine-induced or carbon tetrachloride-induced hepato-toxicity. Digestion 1982; 25: 222- 229.

Yıl 2008, Cilt: 71 Sayı: 2, 29 - 32, 14.11.2011

Şule Seçkin Şeyda Alsancak Canan Başaran Küçükergin Makbule Aydın Şule Seçkin At All.

Öz

Kaynakça

  • Adamson GM, Billings RE. Tumor necrosis factor induced oxi- dative stress in isolated mouse hepatocytes. Arch Biochem Bi- ophys 1992; 294: 223-229.
  • Beutler E, Duron O, Kelly BM. Improved method for the deter- mination of blood glutathione. J Lab Clin Med 1963; 61: 882- 888.
  • Camussi G, Albano E, Tetta C., Bussolino F. The molecular ac- tion of tumor necrosis factor-a. Eur J Biochem 1991; 202: 3-14.
  • Cheng C C, Etoh J, Tanimura T, Egashira Y, Ohta T, Sanada H. Effects of dietary gluten on the hepatotoxic action of galactosa- mine and/or endotoxin in rats. Biosci Biotech Biochem 1996; 60: 439-443.
  • Endo Y, Kikuchi T, Nakamura M. Ornithine and histidine decar- boxylase activities in mice sensitised to endotoxin, interleukin-1 or tumour necrosis factor by D-galactosamine. Br J Pharmacol 1992; 107: 888-894.
  • Hu H, Chen R. Changes in free radicals, trace elements and ne- urophysiological function in rats with liver damage induced by D-galactosamine. Biol Tr Elem Res 1992; 34: 20-25.
  • Irita K, Okabe A, Koga A, Kurosawa K. Increased sinusoidal eff- lux of reduced and oxidized glutathione in rats with endoto- xin/D-galactosamine hepatitis. Circ Shock 1994; 42: 115-120.
  • Itokazu Y, Segawa Y, Inoue N, Omata T. D-Galactosamine-indu- ced mopuse hepatic apoptosis: possible involvement with tumor necrosis factor, but not with caspase-3 activity. Biol Pharm Bull 1999; 22 : 1127-1130.
  • Ledda-Columbano GM, Con P, Curto M.Induction of two diffe- rent modes of cell death, apoptosis and necrosis, in rat liver after a single dose of thioacetamide. Am J Pathol 1991; 139: 1099- 1109.
  • Louis H, Le Moine O, Peny M, Gulbis B. Hepatoprotective ro- le of interleukin 10 in galactosamine-lipopolysaccharide mouse liver injury. Gastroenterology 1997; 112: 935-942.
  • Lowry OH, Rosebrough NJ, Forry AL., Randall R J.Protein me- asurement with the folin phenol reagent. J Biol Chem 1951; 193: 265-275.
  • McMilla J, JollowDJ. Galactos-amine hepatotoxicity: Effect of galactosamine on glutathione resynthesis in rat primary hepa- tocyte cultures. Toxicol Appl Pharmacol 1992; 115: 234-240.
  • Miller S A, Dykes DD, Polesky HF. A simple salting out proce- dure for extracting DNA from human nucleated cells. Nucl Acids Res 1988; 16: 1215.
  • Molina PE, Abumrad NN. Differential effects of hemorrhage and LPS on tissue TNF-a, IL-1 and associate neuro-hormonal and opioid alterations. Life Sci 2000; 66: 399-409.
  • Muntane J, Montero JL., Marchal T, Perez-Seoane C, Lozano JM, Fraga E, Pintado CO. De la Mata M, Mino G. Effect of PGE1 on TNF-a status and hepatic D-galactosamine-induced apoptosis in rats. J Gastroenterol Hepatol 1998; 13, 197-207.
  • Nagakawa, J, Hitota K, Hishinuma I, Miyammoto K. Protective effect of E3330, a novel of quinone derivative, in galactosami- ne-induced hepatitis in rats. J Pharmacol Exp 1993. Ther 264; 496-500.
  • Ohta Y, Matsura T, Kitagawa A, Tokunaga K, Yamada K. Xant- hine oxidase-derived reactive oxygen species contribute to the development of D-galactosamine-induced liver injury in rats. Free Radic Res 2007; 41: 135-144.
  • Okhawa H, Ohishi N, Yagi K) Assay for lipid peroxides in ani- mal tissues by thiobarbituric acid. Anal Biochem 1979; 95: 351- 358.
  • Penning LC, Schipper RG, Vercammen D. Sensitization of TNF- induced apoptosis with polyamine synthesis inhibitors in diffe- rent human and murine tumour cell lines. Cytokine 1998; 10: 423-431.
  • Portoles MT, Catala M, Anton ARHepatic response to the oxi- dative stress induced by E. coli endotoxin: Glutathione as an in- dex of the acute phase during the endotoxic shock. Mol Cell Bi- ochem 1996; 159: 115-121.
  • Rikans LE, Decicco L.A, Hornbrook KR, Yamano T. Effect of age and carbon tetrachloride on cytokine concentrations in rat li- ver. Mech Ageing Develop.1999; 108: 173-182.
  • Santucci L, Fiorucci S, Chiorean M, Brunori P M) Interleukin 10 reduces lethality and hepatic injury induced by lipopolysaccha- ride in galactosamine - sensitized mice. Gastroenterology 1996; 111: 736-744.
  • Seçkin Ş , Alptekin N, Toker NK, Uysal M, Toker GA. Hepatic gamma-glutamyl cysteine synthetase and gamma-glutamyl transpeptidase activities in galactosamine-treated rats. Res Com- mun Mol Pathol Pharmacol 1995; 87: 237-240.
  • Shen W, Kamendulis LM, Ray SD, Corcoran GB. Acetaminop- hen--induced cytotoxicity in cultured mouse hepatocytes: corre- lation of nuclear Ca2+ accumulation and early DNA fragmenta- tion with cell death. Toxicol. Appl Pharmacol 1991; 111: 242- 254.
  • Stachlewitz RF, Seabra V, Bradford B, Bradham C A, Rusyn I, Germolec D, Thurman RG.) Glycine and uridine prevent D-ga- lactosamine hepatotoxicity in the rat: Role of Kupffer cells. He- patology 1999; 29: 737-745.
  • Sun F, Hamagawa E, Tsutsu, C, Sakaguchi N, Kakuta Y, Toku- maru S, Kojo S. Evaluation of oxidative stress during apoptosis and necrosis caused by D-galactosamine in rat liver. Biochem Pharmacol 2003; 65: 101-107.
  • Tsutsu, S, Hirasawa K, Takeda M, Itagaki S, Kawamura S, Ma- eda K. Apoptosis of murine hepatocytes induced by high doses of galactosamine. J Vet Med Sci 1997; 59: 785-790.
  • Tsutsui S, Hirasawa K, Takeda M, Itagaki S, Kawamura S, Ma- eda K, Mikami T, Doi K Galactosamine-induced apoptosis in the primary mouse hepatocyte cultures. Exp Toxic Pathol 1997; 49: 301-306.
  • Wang H, Xu D, Lv J, Ning H, Wie W. Melatonin attenuates li- popolysaccharide (LPS)-induced apoptotic liver damage in D- galactosamine-sensitized mice. Toxicology 2007; 237: 49-57.
  • Yoshikawa T, Furukawa Y, Murakami M, Takemura S, Kondo M. Effect of vitamin E on D-Galactosamine-induced or carbon tetrachloride-induced hepato-toxicity. Digestion 1982; 25: 222- 229.
Toplam 30 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Bölüm Klinik Araştırma
Yazarlar

Şule Seçkin Bu kişi benim

Şeyda Alsancak Bu kişi benim

Canan Başaran Küçükergin Bu kişi benim

Makbule Aydın Bu kişi benim

Şule Seçkin Bu kişi benim

At All. Bu kişi benim

Yayımlanma Tarihi 14 Kasım 2011
Gönderilme Tarihi 14 Kasım 2011
Yayımlandığı Sayı Yıl 2008 Cilt: 71 Sayı: 2

Kaynak Göster

APA Seçkin, Ş., Alsancak, Ş., Başaran Küçükergin, C., Aydın, M., vd. (2011). SIÇANLARIN KARACİĞERLERİNDE D-GALAKTOZAMİNİN OKSİDATİF STRES VE APOPTOZ ÜZERİNE ETKİSİ. Journal of Istanbul Faculty of Medicine, 71(2), 29-32.
AMA Seçkin Ş, Alsancak Ş, Başaran Küçükergin C, Aydın M, Seçkin Ş, All. A. SIÇANLARIN KARACİĞERLERİNDE D-GALAKTOZAMİNİN OKSİDATİF STRES VE APOPTOZ ÜZERİNE ETKİSİ. İst Tıp Fak Derg. Kasım 2011;71(2):29-32.
Chicago Seçkin, Şule, Şeyda Alsancak, Canan Başaran Küçükergin, Makbule Aydın, Şule Seçkin, ve At All. “SIÇANLARIN KARACİĞERLERİNDE D-GALAKTOZAMİNİN OKSİDATİF STRES VE APOPTOZ ÜZERİNE ETKİSİ”. Journal of Istanbul Faculty of Medicine 71, sy. 2 (Kasım 2011): 29-32.
EndNote Seçkin Ş, Alsancak Ş, Başaran Küçükergin C, Aydın M, Seçkin Ş, All. A (01 Kasım 2011) SIÇANLARIN KARACİĞERLERİNDE D-GALAKTOZAMİNİN OKSİDATİF STRES VE APOPTOZ ÜZERİNE ETKİSİ. Journal of Istanbul Faculty of Medicine 71 2 29–32.
IEEE Ş. Seçkin, Ş. Alsancak, C. Başaran Küçükergin, M. Aydın, Ş. Seçkin, ve A. All., “SIÇANLARIN KARACİĞERLERİNDE D-GALAKTOZAMİNİN OKSİDATİF STRES VE APOPTOZ ÜZERİNE ETKİSİ”, İst Tıp Fak Derg, c. 71, sy. 2, ss. 29–32, 2011.
ISNAD Seçkin, Şule vd. “SIÇANLARIN KARACİĞERLERİNDE D-GALAKTOZAMİNİN OKSİDATİF STRES VE APOPTOZ ÜZERİNE ETKİSİ”. Journal of Istanbul Faculty of Medicine 71/2 (Kasım 2011), 29-32.
JAMA Seçkin Ş, Alsancak Ş, Başaran Küçükergin C, Aydın M, Seçkin Ş, All. A. SIÇANLARIN KARACİĞERLERİNDE D-GALAKTOZAMİNİN OKSİDATİF STRES VE APOPTOZ ÜZERİNE ETKİSİ. İst Tıp Fak Derg. 2011;71:29–32.
MLA Seçkin, Şule vd. “SIÇANLARIN KARACİĞERLERİNDE D-GALAKTOZAMİNİN OKSİDATİF STRES VE APOPTOZ ÜZERİNE ETKİSİ”. Journal of Istanbul Faculty of Medicine, c. 71, sy. 2, 2011, ss. 29-32.
Vancouver Seçkin Ş, Alsancak Ş, Başaran Küçükergin C, Aydın M, Seçkin Ş, All. A. SIÇANLARIN KARACİĞERLERİNDE D-GALAKTOZAMİNİN OKSİDATİF STRES VE APOPTOZ ÜZERİNE ETKİSİ. İst Tıp Fak Derg. 2011;71(2):29-32.

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