İHRACATA YÖNELİK ÜRETİLEN BAZI GIDALARDA LISTERIA MONOCYTOGENES VARLIGININ ARAŞTIRILMASI

Yıl 2007, Cilt: 33 Sayı: 2, 1 - 11, 26.11.2012

Solmaz Lee Figen Çetinkaya G. Soyutemiz

Öz

ÖZET

Çalışma, Bursa'da ihracata yönelik üretilen çeşitli deniz ürünleri ve sebzelerde L. monocytogenes'in varlığını belirlemek amacıyla gerçekleştirildi. İncelenen 110 adet örneğin 20 (%18.2)'sinde Listeria türleri belirlendi. Listeria spp. 2 dondurulmuş salyangoz, 15 dondurulmuş biber, 2 dondurulmuş çilek ve 1 dondurulmuş Brüksel lahanasından izole edildi. Dondurulmuş biber örneklerinden alınan 15 Listeria izolatından 3'ü L. monocytogenes olarak identifiye edildi. Çalışmanın sonuçları, dondurulmuş biberlerin L. monocytogenes yönünden sağlık riski oluşturabileceğini ve dondurulmuş biber işleyen işletmelerin, L. monocytogenes'le olası bir kontaminasyonun kontrolü amacıyla, HACCP planlarında gerekli önlemleri almaları gerektiğini ortaya koymuştur.

Anahtar Kelimeler: Listeria spp., Listeria monocytogenes, su ürünleri, sebzeler

 

 

INVESTIGATION ON THE PRESENCE OF LISTERIA MONOCYTOGENES IN SOME FOODS PRODUCED FOR EXPORTING

ABSTRACT

The study was carried out to determine the presence of L. monocytogenes in various seafoods and vegetables produced for exporting in Bursa (Turkey). Of 110 samples analyzed, 20 (18.2%) were found to be Listeria species. Listeria spp. were isolated from 2 frozen snail meats, 15 frozen peppers, 2 frozen strawberries and 1 frozen brussels cabbage. Among 15 Listeria isolates obtained from frozen peppers, three was identified as L. monocytogenes. The results of this study indicated that frozen peppers may have potentially health risk, and frozen pepper producers must take necessary precautions as indicated by HACCP principles to control possible contamination by L. monocytogenes.

Key Words: Listeria spp., Listeria monocytogenes, seafood, vegetables

Anahtar Kelimeler

Listeria spp., Listeria monocytogenes, su ürünleri, sebzeler

Kaynakça

• Adesiyun, A.A.: Prevalence of Listeria spp., Campylobacter spp., Salmonella spp. and toxigenic Escherichia coli on meat and seafoods in Trinidad. Food Microbiol., 1993; 10: 395-403.
• Aguado, V., Vitas, A.I., Garcia-Jalón, I.: Characterization of Listeria monocytogenes and Listeria innocua from a vegetable processing plant by RAPD and REA. Int. J. Food Microbiol., 2004; 90: 341-347.
• Ben Embarek, P.K.: Presence, detection and growth of Listeria monocytogenes in seafoods: a review. Int. J. Food Microbiol., 1994; 23: 17-34.
• Brett, M.S.Y., Short, B., McLauchlin, J.: A small outbreak of listeriosis associated with smoked mussels. Int. J. Food Microbiol., 1998; 43: 223-229.
• Buchanan, R., Stahl, H., Bencivengo, M., Del Corral, F.: Comparison of lithium chloride- phenylethanol-moxalactam and modified Voges Johnson agars for detection of Listeria spp. in retail level meat, poultry and seafood. Appl. Environ. Microbiol., 1989; 55: 599-603.
• CDC. Centers for Disease Control and Prevention. Surveillance for foodborne-disease outbreaks-United States, 1988-1992. Morb. Mortal. Wkly. Rep., 1996; 45: 1-55.
• CDC. Centers for Disease Control and Prevention. Surveillance for foodborne-disease outbreaks-United States, 1993-1997. Morb. Mortal. Wkly. Rep., 2000; 49: 1-51.
• Chasseignaux, E., Gerault, P., Toquin, M.-T., Salvat, G., Colin, P., Ermel, G.: Ecology of Listeria monocytogenes in the environment of raw poultry meat and raw pork meat processing plants. FEMS Microbiol. Lett., 2002; 210: 271-275.
• Colburn, K.G., Kaysner, C., Abeyta, C., Wekell, M.: Listeria species in a California coast estuarine environment. Appl. Environ. Microbiol., 1990; 56: 2007-2011.
• Copes, J., Pellicer, K., Echeverria, H.G., Stanchi, N.O., Martinez, C., Leardini, N.: Investigation of Listeria monocytogenes in soft cheeses. Rev. Argent. Microbiol., 2000; 32: 49-52.
• Decastelli, L., Ercolini, C., Fisichellas, S., Bianchi, C.: Incidenza di Listeria spp. in mitili (Mytilus galloprovincialis) di allevamento. Microbiologie Aliments Nutrition., 1993; 11: 51- 56.
• De Simon, M., Tarrago, C., Ferrer, M.: Incidence of Listeria monocytogenes in fresh foods in Barcelona (Spain). Int. J. Food Microbiol., 1992; 16: 153-156.
• Dhanashree, B., Otta, S.K., Karunasagar, I., Goebel, W., Karunasagar, I.: Incidence of Listeria spp. in clinical and food samples in Mangalore, India. Food Microbiol., 2003; 20: 447-453.
• Doganay, M.: Listeriosis: clinical presentation. FEMS Immunol. Med. Microbiol., 2003; 35: 173-175.
• Eleftheriadou, M., Varnava-Tello, A., Meta-Loizidou, M., Nikolaou, A.S., Akkelidou, D.: The microbiological profile of foods in the Republic of Cyprus: 1991-2000. Food Microbiol., 2002; 19: 463-471.
• Ericsson, H., Eklöw, A., Danielsson-Tham, M.-L., Loncarevic, S., Mentzing, L.-O., Persson, I., Unnerstad, H., Tham, W.: An outbreak of listeriosis suspected to have been caused by rainbow trout. J. Clin. Microbiol., 1997; 35: 2904-2907.
• Farber, J.M., Peterkin, P.I.: Listeria monocytogenes, a foodborne pathogen. Microbiol. Rev., 1991; 55: 476-511.
• Farber, J.M., Sanders, G.W., Johnston, M.A.: A survey of various foods for the presence of Listeria species. J. Food Protect., 1989; 52: 456-458.
• Fenlon, D.R., Wilson, J., Donachie, W.: The incidence and level of Listeria monocytogenes contamination of food sources at primary production and initial processing. J. Appl. Bacteriol., 1996; 81: 641-650.
• Flessa, S, Lusk, D.M., Harris, L.J.: Survival of Listeria monocytogenes on fresh and frozen strawberries. Int. J. Food Microbiol., 2005; 101: 255-262.
• Fuchs, R., Surendran, P.: Incidence of Listeria in tropical fish and fishery products. Lett. Appl. Microbiol., 1989; 9: 49-51.
• Genigeorgis, C., Carniciu, M., Dutulescu, D., Farveri T.B.: Growth and survival of Listeria monocytogenes in market cheeses stored at 4 and 30ºC. J. Food Protect., 1991; 54: 662-668.
• Gombas, D.E., Chen, Y., Clavero, R.S., Scott, V.N.: Survey of Listeria monocytogenes in ready-to-eat foods. J. Food Protect., 2003; 66: 559-569.
• Greenwood, M.H., Roberts, D., Burden, P.: The occurence of Listeria species in milk and dairy products: a national survey in England and Wales. Int. J. Food Microbiol., 1991; 12: 197-206.
• Handa, S., Kimura, B., Takahashi, H., Koda, T., Hisa, K., Fuji, T.: Incidence of Listeria monocytogenes in raw seafood products in Japanese retail stores. J. Food Protect., 2005; 68: 411-415.
• Harris, L.J., Farber, J.N., Beuchat, L.R., Parish, M.E., Suslow, T.V., Garrett, E.H., Busta, F.F.: Outbreaks associated with fresh produce: incidence, growth, and survival of pathogens in fresh and fresh-cut produce. Compr. Rev. Food Sci. Food Saf., 2003; 2: 78-141.
• Hartemink, R., Georgsson, G.: Incidence of Listeria species in seafood and seafood salads. Int. J. Food Microbiol., 1991; 12: 189-195.
• Heintiz, M.L., Johnson, J.M.: The incidence of Listeria spp., Salmonella spp. and Clostridium botulinum in smoked fish and shellfish. J. Food Protect., 1998; 61: 318-323.
• Heisick, J.E., Wagner, D.E., Nierman, M.L., Peeler, J.T.: Listeria spp. found on fresh market produce. Appl. Environ. Microbiol., 1989; 55: 1925-1927.
• Hitchins, A.D.: Listeria monocytogenes, FDA Bacteriological Analytical Manual. 1995; AOAC International, Gaithersburg.
• Inoue, S., Nakama, A., Arai, Y., Kokubo, Y., Maruyama, T., Saito, A., Yoshida, T., Terao, M., Yamamoto, S., Kumagai, S.: Prevalence and contamination levels of Listeria monocytogenes in retail foods in Japan. Int. J. Food Microbiol., 2000; 59: 73-77.
• Jeyasekaran, G., Karunasagar, I., Karunasagar, I.: Incidence of Listeria spp. in tropical fish. Int. J. Food Microbiol., 1996; 31: 333-340.
• Johnston, L.M., Jaykus, L.A., Moll, D., Martinez, M.C., Anciso, J., Mora, B., Moe, C.L.: A field study of the microbiological quality of fresh produce. J. Food Protect., 2005; 68: 1840-1847.
• Jİrgensen, L.V., Huss, H.H.: Prevalence and growth of Listeria monocytogenes in naturally contaminated seafood. Int. J. Food Microbiol., 1998; 42: 127-131.
• Kamat, A.A., Nair, P.M.: Incidence of Listeria species in Indian seafoods and meat. J. Food Saf., 1994; 14: 117-130.
• Klima, R.A., Montville, T.J.: The regulatory and industry responses to listeriosis in the USA: a paradigm for dealing with emerging foodborne pathogens. Trends Food Sci. Technol., 1995 ; 6 : 87-93.
• Laciar, A.L., de Centorbi, O.N.P.: Listeria species in seafood: isolation and characterization of Listeria spp. from seafood in San Luis, Argentina. Food Microbiol., 2002; 19: 645-651.
• Lee, S., Cetinkaya, F., Soyutemiz, G.E.: Occurrence of Listeria species in the processing stages of frozen pepper. J. Food Saf., (Baskıda).
• Manoj, Y.B., Rosalind, G.M., Karunasagar, I., Karunasagar, I.: Listeria spp. in fish and fish handling areas, Mangolore, India. Asian Fish. Sci., 1991; 4: 119-122.
• Marth, E.H.: Growth and survival of Listeria monocytogenes, Salmonella species, and Staphylococcus aureus in the presence of sodium chloride. Dairy Food Environ. San., 1993; 13: 14-18.
• Masuda, T., Iwaya, M., Miura, H., Kokubo, Y., Marumaya, T.: Occurrence of Listeria species in fresh seafoods. J. Food Hyg. Soc. Japan., 1992; 33: 599-602. 10
• Mena, C., Almeida, G., Carneiro, L., Teixeira, P., Hogg, T, Gibbs, P.A.: Incidence of Listeria monocytogenes in different food products commercialized in Portugal. Food Microbiol., 2004; 21: 213-216.
• Miettinen, M.K., Siitonen, A., Heiskanen, P., Haajanen, H., Bjorkroth, K.J., Korkeala, H.J.: Molecular epidemiology of an outbreak of febrile gastroenteritis caused by Listeria monocytogenes in cold-smoked rainbow trout. J. Clin. Microbiol., 1999; 37: 2358-2360.
• Monge, R., Arias, M.L.: Presence of various pathogenic microorganisms in fresh vegetables in Costa Rica. Arch. Latinoam. Nutr., 1996; 46: 292-294.
• Motes, M.: Incidence of Listeria spp. in shrimps, oysters and estuarine waters. J. Food Protect., 1991; 54: 170-173.
• Nakamura, H., Hatanaka, M., Ochi, K., Nagao, M., Ogasawara, J., Hase, A., Kitase, T., Haruki, K., Nishikawa, Y.: Listeria monocytogenes isolated from cold-smoked fish products in Osaka city, Japan. Int. J. Food Microbiol., 2004; 94: 323-328.
• Nİrrung, B., Andersen, J.K., Schlundt, J.: Incidence and control of Listeria monocytogenes in foods in Denmark. Int. J. Food Microbiol., 1999; 53: 195-203.
• Odumeru, J.A., Mitchell, S.J., Alves, D.M., Lynch, J.A., Yee, A.J., Wang, S.L., Styliadis, S., Farber, J.M.: Assessment of the microbiological quality of ready-to-use vegetables for health-care food services. J. Food Protect., 1997; 60: 954-960.
• Parish, M.E., Higgins, D.P.: Survival of Listeria monocytogenes in low pH model broth systems. J. Food Protect., 1989; 52: 144-147.
• Prazak, A.M., Murano, E.A., Mercado, I., Acuff, G.R.: Prevalence of Listeria monocytogenes during production and postharvest processing of cabbage. J. Food Protect., 2002; 65: 1728-1734.
• Ripabelli, G., Sammarco, M.L., Fanelli, I., Grasso, G.M.: Detection of Salmonella, Listeria spp., Vibrio spp., and Yersinia enterocolitica in frozen seafood and comparison with enumeration for faecal indicators: implication for public health. Ann Ig., 2004, 16: 531-539.
• Rocourt, J., Jacquet, Ch., Reilly, A.: Epidemiology of human listeriosis and seafoods. Int. J. Food Microbiol., 2000; 62: 197-209.
• Schlech, W.F., Lavigne, P.M., Bortolussi, R.A., Allen, A.C., Haldane, E.V., Wort, A.J., Hightower, A.W., Johnson, S.E., King, S.H., Nicholis, E.S., Broome, C.V.: Epidemic listeriosis-evidence for transmission by food. N. Engl. J. Med., 1983; 308: 203-206.
• Sizmur, K., Walker, C.W.: Listeria in prepacked salads. Lancet I (8595), 1988; 1167.
• Smith, W.: Listeriosis in western Sydney. Public Health Bulletin, 1991; 2: 133-134.
• Soriano, J.M., Rico, H., Moltó, J.C., Manes, J.: Listeria species in raw and ready-to-eat foods from restaurants. J. Food Protect., 2001; 64: 551-553.
• Steinbruegge, E.G., Maxcy, R.B., Liewen, M.B.: Fate of Listeria monocytogenes on ready to serve lettuce. J. Food Protect., 1988; 51: 596-599.
• Uyttendaele, M., De Troy, P., Debevere, J.: Incidence of Listeria monocytogenes in different types of meat products on the Belgian retail market. Int. J. Food Microbiol., 1999; 53: 75-80. 11
• Vitas, A.I., Aguado, V., Garcia-Jalon, I.: Occurrence of Listeria monocytogenes in fresh and processed foods in Navarra (Spain). Int. J. Food Microbiol., 2003; 90: 349-356.
• Weagant, S., Sado, P., Colburn, K., Torkelson, J., Stanley, F., Krane, M., Shields, S., Thayer, C.: The incidence of Listeria species in frozen seafood products. J. Food Protect., 1988; 51: 655-657.
• Yücel, N., Çıtak, S., Önder, M.: Prevalence and antibiotic resistance of Listeria species in meat products in Ankara, Turkey. Food Microbiol., 2005; 22: 241-245.