Effects of Berberine and Curcumin on Some Oxidative Stress Parameters of the Bladder in Cyclophosphamide-Induced Interstitial Cystitis

Öz

This study demonstrates the modulation of oxidative stress levels in traumatic conditions by cyclophosphamide (CYP) and berberine (BER) and curcumin (KUR), known for their potent antioxidant properties. A total of 36 female Wistar-Albino mice were randomly divided into six groups (n=6): Control, DMSO, CYP (75 mg/kg), CYP+BER (50 mg/kg), CYP+KUR (50 mg/kg), and CYP+BER+KUR. Oxidative stress levels were analyzed by measuring malondialdehyde (MDA) levels and catalase (CAT) and glutathione (GSH) activities. Cyclophosphamide (CYP) administration resulted in a significant increase in MDA levels, a marker of lipid peroxidation, in bladder tissue. BER and KUR treatments significantly suppressed MDA levels, and enzyme activity remained under control for a normal duration. Synergistic improvement, particularly observed in the combined (BER+KUR) groups, was more pronounced compared to single treatments. In conclusion, the biochemical data revealed that berberine (BER) and curcumin (KUR) administration significantly suppressed lipid peroxidation in cyclophosphamide (CYP) structural injury and repaired endogenous antioxidant defense mechanisms. These findings suggest that these phytochemicals may play a role in protecting the body by limiting oxidative infection and could be considered promising therapeutic agents for the management of interstitial cystitis.

Anahtar Kelimeler

• Cyclophosphamide
• Berberine
• Curcumin
• Interstitial cystitis
• Oxidative stress

Siklofosfamid Kaynaklı İnterstisyel Sistitte Berberin ve Kurkuminin Mesane Üzerine Bazı Oksidatif Stres Parametrelerin Etkileri

Öz

Bu çalışma, siklofosfamid (CYP) kaynaklı mesane hasarında gelişen oksidatif stres yanıtının, güçlü antioksidan özellikleri ile bilinen berberin (BER) ve kurkumin (KUR) ile modüle edilebilirliğini araştırmayı amaçlamaktadır. Çalışmada toplam 36 dişi Wistar-Albino sıçan rastgele altı gruba ayrıldı (n=6): Kontrol, DMSO, CYP (75 mg/kg), CYP+BER (50 mg/kg), CYP+KUR (50 mg/kg) ve CYP+BER+KUR. Oksidatif stres düzeyleri; malondialdehit (MDA) seviyeleri ile katalaz (CAT) ve glutatyon (GSH) aktiviteleri ölçülerek analiz edildi. Siklofosfamid (CYP) uygulaması, mesane dokusunda lipid peroksidasyon belirteci olan MDA düzeylerinde anlamlı bir artış ile sonuçlanmıştır. BER ve KUR tedavileri, MDA düzeyinde anlamlı bir şekilde baskılamış ve antioksidan enzim aktivitelerinin kontrol seviyelerini normal düzeye getirmiştir. Özellikle kombine (BER+KUR) grubunda gözlenen sinerjistik iyileşme, tekli tedavilere göre daha belirgin bulunmuştur. Sonuç olarak, elde edilen biyokimyasal veriler; berberin (BER) ve kurkumin (KUR) uygulamalarının, siklofosfamid (CYP) kaynaklı mesane hasarında lipid peroksidasyonun belirgin şekilde baskı ettiğini ve endojen antioksidan savunma mekanizmalarını restore ettiğini ortaya koymaktadır. Bu bulgular, söz konusu fitokimyasalların oksidatif hasarı sınırlayarak mesane dokusunu korumada stratejik bir rol oynayabileceğine ve interstisyel sistit yönetimi için umut verici terapötik ajanlar olarak değerlendirilebileceğine işaret etmektedir.

Anahtar Kelimeler

• Siklofosfamid
• Berberin
• Kurkumin
• İnterstisyel sistit
• Oksidatif stres

Etik Beyan

Van Yüzüncü Yıl Üniversitesi Hayvan Deneyleri Etik Kurulunun Tarih: 25/12/2025.; Karar No: 2025/13-04 olan izin alınmıştır.

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