Antioxidant effect of Achillea biebersteinii in different extracts and extinguhishing activity on DPPH

Yıl 2021, Cilt: 4 Sayı: 1, 1 - 9, 30.06.2021

Duygu Doğan , İsmet Meydan

https://doi.org/10.46876/ja.915923

Cited By: 1

Öz

Achillea biebersteinii (A. biebersteinii), one of the Achillea species, has been proven to have positive effects in terms of pharmaceuticals thanks to the components it contains. Our study was designed to investigate the quenching activity of A. biebersteinii on total phenolic component, total flavonoid component and DPPH radical. The total flavonoid component amounts of the ethanol and water extract of A. biebersteinii plant were calculated to be equivalent to quercetin, and the total phenol component amounts to gallic acid. The DPPH radical quenching activity of the ethanol and water extract of the A. biebersteinii plant was investigated by modifying the Teixeira method. The copper II ion reduction method (CUPRAC) is inspired by the Apak method. We can say that the water and alcohol extract of A. biebersteinii gave good results compared to BHA and BHT, which have good quenching activity on DPPH radical. It was observed that A. biebersteinii was rich in flavonoids and phenolics. As a result of Cuprac analysis, it was stated that the effect of the aqueous extract was significant compared to the positive controls. The A. biebersteinii plant is thought to be effective in the treatment of many diseases, especially cancer, due to its powerful antioxidant and radical quenching activity.

Anahtar Kelimeler

Achillea biebersteinii, flavonoid, phenolic, cuprac, antioxidant

Achillea biebersteinii'nin Farklı Ekstraktlarda Antioksidan Etkisi ve DPPH Üzerindeki Söndürme Aktivitesi

Öz

Achillea türlerinden biri olan Achillea biebersteinii (A. biebersteinii), içerdiği bileşenler sayesinde farmasötik açıdan olumlu etkilere sahip olduğu kanıtlanmıştır. Çalışmamız, A. biebersteinii'nin toplam fenolik bileşen, toplam flavonoid bileşen ve DPPH radikali üzerindeki söndürme aktivitesini araştırmak için tasarlanmıştır. A. biebersteinii bitkisinin etanol ve su ekstraktının toplam flavonoid bileşen miktarları quercetin'e, toplam fenol bileşen miktarları ise gallik aside eşdeğer olarak hesaplandı. A. biebersteinii bitkisinin etanol ve su özütünün DPPH radikal söndürme aktivitesi Teixeira yöntemi değiştirilerek araştırıldı. Bakır II iyon azaltma yöntemi (CUPRAC), Apak ve arkadaşlarının çalışmalarından esinlenerek yapılmıştır. A. biebersteinii'nin su ve alkol özütünün, DPPH radikali üzerinde iyi söndürme aktivitesine sahip olan BHA ve BHT'ye kıyasla iyi sonuçlar verdiğini söyleyebiliriz. A. biebersteinii' nin flavonoidler ve fenolikler açısından zengin olduğunu görüldü. Cuprac analizi sonucunda sulu ekstrenin etkisinin pozitif kontrollere göre önemli olduğu belirtildi. A. biebersteinii bitkisinin, güçlü antioksidan ve radikal söndürme aktivitesinden dolayı başta kanser olmak üzere birçok hastalığın tedavisinde etkili olduğu düşünülmektedir.

Anahtar Kelimeler

Achillea biebersteinii, Fenolik, Flavonoid, Kuprak, Antioksidan

Kaynakça

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