Effect of nigella sativa oil on bisphenol a-induced hepatotoxicity in wistar albino rats: histopathological and biochemical investigation

Mehmet Burak Ateş; Durmuş Hatipoğlu

doi:10.31015/jaefs.2022.3.9

EN

Effect of nigella sativa oil on bisphenol a-induced hepatotoxicity in wistar albino rats: histopathological and biochemical investigation

Abstract

Bisphenol A (or BPA) is a toxic endocrine disruptor that is emitted into the environment as a result of industrial manufacturing methods. In this research, we focused on investigating the protective effects of Nigella sativa oil (NSO) on the liver in rats treated with hepatotoxic BPA. For this purpose, 30 Wistar Albino rats were divided into 4 groups: Control (1 ml olive oil); NSO (5 ml/kg NSO); BPA (100mg/kg); BPA+ NSO (100 mg/kg BPA + 5 ml/kg NSO). All applications were done by oral gavage. At the end of the 30-day study period, blood samples of the anesthetized rats were collected and euthanized under appropriate conditions. After removing the serum of the collected blood samples, alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) and gamma glutamyl transferase (GGT) levels, which have a key role in liver toxicity, were measured. At the same time, liver samples that were dissected and removed from the cadaver were fixed in 10% formaldehyde solution for histopathological examination and scoring, and hematoxylin - eosin staining were performed. BPA caused degeneration and necrosis in hepatocytes, Kuffper activation, bile duct hyperplasia, congestion, and hepatic cord dissociation, causing serious increases in total liver lesion scores. In parallel, BPA-induced increases were detected in ALT, AST, ALP, and GGT levels. The histological architecture and liver function tests were significantly improved with the addition of NSO to the diet. These findings provided that NSO has a hepatoprotective effect by improving BPA-induced liver damage.

Keywords

BPA, Black Cumin, Hepatotoxicity, Antioxidants, Pathology

References

Abdel-Zaher, A. O., Abdel-Rahman, M. S., & Elwasei, F. M. (2010). Blockade of Nitric Oxide Overproduction and Oxidative Stress by Nigella sativa Oil Attenuates Morphine-Induced Tolerance and Dependence in Mice. Neurochemical Research, 35(10), 1557-1565. DOI: https://doi.org/10.1007/s11064-010-0215-2
Abdel Samie, H. A., Nassar, S. A., & Hussein, Y. (2018). Ameliorative Potential of Selenium against Bisphenol A- Induced Hepatotoxicity in Rats. The Egyptian Journal of Hospital Medicine, 67(1), 444-454. DOI: https://ejhm.journals.ekb.eg/article_14019_29af1d727d495f06df34b02417c02d81.pdf
Al-Seeni, M. N., El Rabey, H. A., Zamzami, M. A., & Alnefayee, A. M. (2016). The hepatoprotective activity of olive oil and Nigella sativa oil against CCl4 induced hepatotoxicity in male rats. BMC Complementary and Alternative Medicine, 16(1), 438. DOI: https://doi.org/10.1186/s12906-016-1422-4
Ates, M. B., & Ortatatli, M. (2021a). The effects of Nigella sativa seeds and thymoquinone on aflatoxin phase-2 detoxification through glutathione and glutathione-S-transferase alpha-3, and the relationship between aflatoxin B1-DNA adducts in broilers. Toxicon, 193, 86-92. DOI: https://doi.org/10.1016/j.toxicon.2021.01.020
Ates, M. B., & Ortatatli, M. (2021b). Phase-1 bioactivation mechanisms of aflatoxin through AhR, CAR and PXR nuclear receptors and the interactions with Nigella sativa seeds and thymoquinone in broilers. Ecotoxicology and Environmental Safety, 208, 111774. DOI: https://doi.org/10.1016/j.ecoenv.2020.111774
Ates, M. B., Ortatatli, M., Oguz, H., Ozdemir, O., Terzi, F., Ciftci, M. K., & Hatipoglu, F. (2022). The ameliorative effects of Nigella sativa, thymoquinone, and bentonite against aflatoxicosis in broilers via AFAR and Nrf2 signalling pathways, and down-regulation of caspase-3. British Poultry Science, 63(3), 332-339. DOI: https://doi.org/10.1080/00071668.2021.1998366
Bindhumol, V., Chitra, K. C., & Mathur, P. P. (2003). Bisphenol A induces reactive oxygen species generation in the liver of male rats. Toxicology, 188(2-3), 117-124. DOI: https://doi.org/10.1016/s0300-483x(03)00056-8
Bordbar, H., Soleymani, F., Nadimi, E., Yahyavi, S. S., & Fazelian-Dehkordi, K. (2021). A Quantitative Study on the Protective Effects of Resveratrol against Bisphenol A-induced Hepatotoxicity in Rats: A Stereological Study. Iranian journal of medical sciences, 46(3), 218-227. DOI: https://doi.org/10.30476/ijms.2020.83308.1233
Center, S. A. (2007). Interpretation of Liver Enzymes. Veterinary Clinics of North America: Small Animal Practice, 37(2), 297-333. DOI: https://doi.org/https://doi.org/10.1016/j.cvsm.2006.11.009
Diamante, G., Cely, I., Zamora, Z., Ding, J., Blencowe, M., Lang, J., . . . Yang, X. (2021). Systems toxicogenomics of prenatal low-dose BPA exposure on liver metabolic pathways, gut microbiota, and metabolic health in mice. Environment International, 146, 106260. DOI: https://doi.org/https://doi.org/10.1016/j.envint.2020.106260

Doherty, L. F., Bromer, J. G., Zhou, Y., Aldad, T. S., & Taylor, H. S. (2010). In utero exposure to diethylstilbestrol (DES) or bisphenol-A (BPA) increases EZH2 expression in the mammary gland: an epigenetic mechanism linking endocrine disruptors to breast cancer. Hormones and Cancer, 1(3), 146-155. DOI: https://doi.org/10.1007/s12672-010-0015-9
Geens, T., Roosens, L., Neels, H., & Covaci, A. (2009). Assessment of human exposure to Bisphenol-A, Triclosan and Tetrabromobisphenol-A through indoor dust intake in Belgium. Chemosphere, 76(6), 755-760. DOI: https://doi.org/10.1016/j.chemosphere.2009.05.024
Goorden, S. M. I., Buffart, T. E., Bakker, A., & Buijs, M. M. (2013). [Liver disorders in adults: ALT and AST]. Nederlands tijdschrift voor geneeskunde, 157(43), A6443. Retrieved from http://europepmc.org/abstract/MED/24152362
Greaves, P. (2007). Liver and Pancreas. In P. Greaves (Ed.), Histopathology of Preclinical Toxicity Studies (pp. 457-569). Academic Press. DOI: https://doi.org/10.1016/b978-044452771-4/50010-9
Gupta, C., & Prakash, D. (2015). Nutraceuticals for geriatrics. Journal of Traditional and Complementary Medicine, 5(1), 5-14. DOI: https://doi.org/https://doi.org/10.1016/j.jtcme.2014.10.004
Hamza, R. Z., & Al-Harbi, M. S. (2015). Amelioration of paracetamol hepatotoxicity and oxidative stress on mice liver with silymarin and Nigella sativa extract supplements. Asian Pacific Journal of Tropical Biomedicine, 5(7), 521-531. DOI: https://doi.org/https://doi.org/10.1016/j.apjtb.2015.03.011
Han, C., & Hong, Y. C. (2016). Bisphenol A, Hypertension, and Cardiovascular Diseases: Epidemiological, Laboratory, and Clinical Trial Evidence. Current Hypertension Reports, 18(2), 11. DOI: https://doi.org/10.1007/s11906-015-0617-2
Hannan, M. A., Rahman, M. A., Sohag, A. A. M., Uddin, M. J., Dash, R., Sikder, M. H., . . . Kim, B. (2021). Black Cumin (Nigella sativa L.): A Comprehensive Review on Phytochemistry, Health Benefits, Molecular Pharmacology, and Safety. Nutrients, 13(6), 1784. Retrieved from https://www.mdpi.com/2072-6643/13/6/1784
Hassan, Z. K., Elobeid, M. A., Virk, P., Omer, S. A., ElAmin, M., Daghestani, M. H., & AlOlayan, E. M. (2012). Bisphenol A induces hepatotoxicity through oxidative stress in rat model. Oxidative Medicine and Cellular Longevity, 2012, 194829. DOI: https://doi.org/10.1155/2012/194829
Hatipoglu, D., & Keskin, E. (2022a). The effect of curcumin on some cytokines, antioxidants and liver function tests in rats induced by Aflatoxin B1. Heliyon, e09890. DOI: https://doi.org/10.1016/j.heliyon.2022.e09890
Hatipoğlu, D., & Keskin, E. (2022b). Ameliorative Effects of Curcumin on Aflatoxin B1-Induced Nephrotoxicity in Wistar-Albino Rats. Harran University Journal of The Faculty of Veterinary Medicine. 11, 2, 139-140. DOI: https://doi.org/https://dergipark.org.tr/tr/pub/huvfd/issue/70449/1093603
Hoekstra, E. J., & Simoneau, C. (2013). Release of bisphenol A from polycarbonate—a review. Critical Reviews in Food Science and Nutrition, 53(4), 386-402. DOI: https://doi.org/10.1080/10408398.2010.536919
Huang, Y. Q., Wong, C. K., Zheng, J. S., Bouwman, H., Barra, R., Wahlstrom, B., . . . Wong, M. H. (2012). Bisphenol A (BPA) in China: a review of sources, environmental levels, and potential human health impacts. Environment International, 42, 91-99. DOI: https://doi.org/10.1016/j.envint.2011.04.010
İnan, A. O., Durgun, Z., Koca, O., & Hatipoğlu, D. (2021). Effect of Coenzyme Q10 on Plasma Parameters in Hypothyroıd Rats. Harran University Journal of The Faculty of Veterinary Medicine, 10, 66-72. Retrieved from https://dergipark.org.tr/en/pub/huvfd/issue/62942/884704
Iwakiri, Y. (2015). Nitric oxide in liver fibrosis: The role of inducible nitric oxide synthase. Clinical and Molecular Hepatology, 21(4), 319-325. DOI: https://doi.org/10.3350/cmh.2015.21.4.319
Kazmi, S. T. B., Majid, M., Maryam, S., Rahat, A., Ahmed, M., Khan, M. R., & Haq, I. U. (2018). Quercus dilatata Lindl. ex Royle ameliorates BPA induced hepatotoxicity in Sprague Dawley rats. Biomedicine Pharmacotherapy, 102, 728-738. DOI: https://doi.org/10.1016/j.biopha.2018.03.097
Kısadere, İ., Faruk Aydın, M., Usta, M., & Donmez, N. (2021). Protective effects of oral melatonin against cadmiuminduced neurotoxicity in Wistar rats. Arhiv za higijenu rada i toksikologiju, 72(2), 157-163. DOI: https://doi.org/https://doi.org/10.2478/aiht-2021-72-3513
Kısadere, İ., Karaman, M., Aydın, M. F., Donmez, N., & Usta, M. (2021). The protective effects of chitosan oligosaccharide (COS) on cadmium-induced neurotoxicity in Wistar rats. Archives of Environmental & Occupational Health, 1-9. DOI: https://doi.org/10.1080/19338244.2021.2008852
Knaak, J. B., & Sullivan, L. J. (1966). Metabolism of bisphenol A in the rat. Toxicology and Applied Pharmacology, 8(2), 175-184. DOI: https://doi.org/10.1016/s0041-008x(66)80001-7
Kooti, W., Hasanzadeh-Noohi, Z., Sharafi-Ahvazi, N., Asadi-Samani, M., & Ashtary-Larky, D. (2016). Phytochemistry, pharmacology, and therapeutic uses of black seed (Nigella sativa). Chinese Journal of Natural Medicines, 14(10), 732-745. DOI: https://doi.org/10.1016/S1875-5364(16)30088-7
Korkmaz, A., Ahbab, M. A., Kolankaya, D., & Barlas, N. (2010). Influence of vitamin C on bisphenol A, nonylphenol and octylphenol induced oxidative damages in liver of male rats. Food and Chemical Toxicology, 48(10), 2865-2871. DOI: https://doi.org/10.1016/j.fct.2010.07.019
Laws, S. C., Carey, S. A., Ferrell, J. M., Bodman, G. J., & Cooper, R. L. (2000). Estrogenic Activity of Octylphenol, Nonylphenol, Bisphenol A and Methoxychlor in Rats. Toxicological Sciences, 54(1), 154-167. DOI: https://doi.org/10.1093/toxsci/54.1.154
Makris, K., Andra, S., Jia, A., Herrick, L., Christophi, C., Snyder, S. A., & Hauser, R. (2013). Association between water consumption from polycarbonate containers and bisphenol A intake during harsh environmental conditions in summer. Environmental science & technology, 47(7), 3333-3343. DOI: https://doi.org/10.1021/es304038k
Meeker, J. D., Calafat, A. M., & Hauser, R. (2010). Urinary bisphenol A concentrations in relation to serum thyroid and reproductive hormone levels in men from an infertility clinic. Environmental Science & Technology, 44(4), 1458-1463. DOI: https://doi.org/10.1021/es9028292
Meng, Z., Tian, S., Yan, J., Jia, M., Yan, S., Li, R., . . . Zhou, Z. (2019). Effects of perinatal exposure to BPA, BPF and BPAF on liver function in male mouse offspring involving in oxidative damage and metabolic disorder. Environmental Pollution, 247, 935-943. DOI: https://doi.org/10.1016/j.envpol.2019.01.116
Michalowicz, J. (2014). Bisphenol A--sources, toxicity and biotransformation. Environmental Toxicology and Pharmacology, 37(2), 738-758. DOI: https://doi.org/10.1016/j.etap.2014.02.003
Moshtaghie, A. A., Javadi, I., & Feghhi, G. (2003). Changes in the Level of Mitochondrial and Cytosolic Aspartate Aminotransferase Activities in Aluminium Intoxified Rat. Iranian Biomedical Journal, 7(4), 167-171. Retrieved from http://ibj.pasteur.ac.ir/article-1-524-en.html
Mukherjee, U., Samanta, A., Biswas, S., Das, S., Ghosh, S., Mandal, D. K., & Maitra, S. (2020). Bisphenol A-induced oxidative stress, hepatotoxicity and altered estrogen receptor expression in Labeo bata: impact on metabolic homeostasis and inflammatory response. Ecotoxicology and Environmental Safety, 202, 110944. DOI: https://doi.org/10.1016/j.ecoenv.2020.110944
Murata, M., & Kang, J. H. (2018). Bisphenol A (BPA) and cell signaling pathways. Biotechnology Advances, 36(1), 311-327. DOI: https://doi.org/10.1016/j.biotechadv.2017.12.002
Nangia, P., & Yadav, V. (2021). Acute Toxicity and Effect of Bisphenol-A Exposure on Serum Alkaline Phosphatase in Channa punctatus. The Scıentıfıc Temper, 24. Retrieved from https://connectjournals.com/03960.2021.12.23
Olukole, S. G., Lanipekun, D. O., Ola-Davies, E. O., & Oke, B. O. (2019). Melatonin attenuates bisphenol A-induced toxicity of the adrenal gland of Wistar rats. Environmental Science and Pollution Research, 26(6), 5971-5982. DOI: https://doi.org/10.1007/s11356-018-4024-5
Reitman, S., & Frankel, S. (1957). A Colorimetric Method for the Determination of Serum Glutamic Oxalacetic and Glutamic Pyruvic Transaminases. American Journal of Clinical Pathology, 28(1), 56-63. DOI: https://doi.org/10.1093/ajcp/28.1.56
Rej, R. (1989). Aminotransferases in Disease. Clinics in Laboratory Medicine, 9(4), 667-687. DOI: https://doi.org/10.1016/S0272-2712(18)30598-5
Salem, M. L. (2005). Immunomodulatory and therapeutic properties of the Nigella sativa L. seed. International Immunopharmacology, 5(13-14), 1749-1770. DOI: https://doi.org/10.1016/j.intimp.2005.06.008
Schug, T. T., Janesick, A., Blumberg, B., & Heindel, J. J. (2011). Endocrine disrupting chemicals and disease susceptibility. The Journal of Steroid Biochemistry and Molecular Biology, 127(3-5), 204-215. DOI: https://doi.org/10.1016/j.jsbmb.2011.08.007
Sharma, U., Pal, D., & Prasad, R. (2014). Alkaline Phosphatase: An Overview. Indian Journal of Clinical Biochemistry, 29(3), 269-278. DOI: https://doi.org/10.1007/s12291-013-0408-y
Sun, Y., Wang, X., Zhou, Y., Zhang, J., Cui, W., Wang, E., . . . Xu, X. (2021). Protective effect of metformin on BPA-induced liver toxicity in rats through upregulation of cystathionine β synthase and cystathionine γ lyase expression. Science of the Total Environment, 750, 141685. DOI: https://doi.org/https://doi.org/10.1016/j.scitotenv.2020.141685
Tarafdar, A., Sirohi, R., Balakumaran, P. A., Reshmy, R., Madhavan, A., Sindhu, R., . . . Sim, S. J. (2022). The hazardous threat of Bisphenol A: Toxicity, detection and remediation. Journal of Hazardous Materials, 423(Pt A), 127097. DOI: https://doi.org/10.1016/j.jhazmat.2021.127097
Tian, X., Liu, Y., Liu, X., Gao, S., & Sun, X. (2019). Glycyrrhizic acid ammonium salt alleviates Concanavalin A-induced immunological liver injury in mice through the regulation of the balance of immune cells and the inhibition of hepatocyte apoptosis. Biomedicine & Pharmacotherapy, 120, 109481. DOI: https://doi.org/https://doi.org/10.1016/j.biopha.2019.109481
Uzunhisarcikli, M., & Aslanturk, A. (2019). Hepatoprotective effects of curcumin and taurine against bisphenol A-induced liver injury in rats. Environmental Science and Pollution Research, 26(36), 37242-37253. DOI: https://doi.org/10.1007/s11356-019-06615-8
Wenk, M. R., & Fernandis, A. Z. (2007). A manual for biochemistry protocols (Vol. 3). World Scientific. (World Scientific Publishing Co. Pte. Ltd). DOI: https://doi.org/10.1142/6269
Whitfield, J. B. (2001). Gamma Glutamyl Transferase. Critical Reviews in Clinical Laboratory Sciences, 38(4), 263-355. DOI: https://doi.org/10.1080/20014091084227
Zaulet, M., Kevorkian, S. E. M., Dinescu, S., Cotoraci, C., Suciu, M., Herman, H., . . . Hermenean, A. (2017). Protective effects of silymarin against bisphenol A-induced hepatotoxicity in mouse liver. Experimental and Therapeutic Medicine, 13(3), 821-828. DOI: https://doi.org/10.3892/etm.2017.4066
Zhang, H., Ju, Q., Pang, S., Wei, N., & Zhang, Y. (2021). Recent progress of fluorescent probes for the detection of alkaline phosphatase (ALP): A review. Dyes and Pigments, 194, 109569. DOI: https://doi.org/https://doi.org/10.1016/j.dyepig.2021.109569
Ziv-Gal, A., Craig, Z. R., Wang, W., & Flaws, J. A. (2013). Bisphenol A inhibits cultured mouse ovarian follicle growth partially via the aryl hydrocarbon receptor signaling pathway. Reproductive Toxicology, 42, 58-67. DOI: https://doi.org/10.1016/j.reprotox.2013.07.022

Details

Primary Language

English

Subjects

Veterinary Sciences, Veterinary Surgery

Journal Section

Research Article

Authors

Mehmet Burak Ateş ^*
0000-0003-1297-426X
Türkiye

Durmuş Hatipoğlu
0000-0003-3790-7821
Türkiye

Publication Date

September 23, 2022

Submission Date

June 1, 2022

Acceptance Date

July 15, 2022

Published in Issue

Year 2022 Volume: 6 Number: 3

DOI

https://doi.org/10.31015/jaefs.2022.3.9

IZ

https://izlik.org/JA53ZZ84KM

APA

Ateş, M. B., & Hatipoğlu, D. (2022). Effect of nigella sativa oil on bisphenol a-induced hepatotoxicity in wistar albino rats: histopathological and biochemical investigation. International Journal of Agriculture Environment and Food Sciences, 6(3), 402-409. https://doi.org/10.31015/jaefs.2022.3.9

AMA

1.Ateş MB, Hatipoğlu D. Effect of nigella sativa oil on bisphenol a-induced hepatotoxicity in wistar albino rats: histopathological and biochemical investigation. int. j. agric. environ. food sci. 2022;6(3):402-409. doi:10.31015/jaefs.2022.3.9

Chicago

Ateş, Mehmet Burak, and Durmuş Hatipoğlu. 2022. “Effect of Nigella Sativa Oil on Bisphenol A-Induced Hepatotoxicity in Wistar Albino Rats: Histopathological and Biochemical Investigation”. International Journal of Agriculture Environment and Food Sciences 6 (3): 402-9. https://doi.org/10.31015/jaefs.2022.3.9.

EndNote

Ateş MB, Hatipoğlu D (September 1, 2022) Effect of nigella sativa oil on bisphenol a-induced hepatotoxicity in wistar albino rats: histopathological and biochemical investigation. International Journal of Agriculture Environment and Food Sciences 6 3 402–409.

IEEE

[1]M. B. Ateş and D. Hatipoğlu, “Effect of nigella sativa oil on bisphenol a-induced hepatotoxicity in wistar albino rats: histopathological and biochemical investigation”, int. j. agric. environ. food sci., vol. 6, no. 3, pp. 402–409, Sept. 2022, doi: 10.31015/jaefs.2022.3.9.

ISNAD

Ateş, Mehmet Burak - Hatipoğlu, Durmuş. “Effect of Nigella Sativa Oil on Bisphenol A-Induced Hepatotoxicity in Wistar Albino Rats: Histopathological and Biochemical Investigation”. International Journal of Agriculture Environment and Food Sciences 6/3 (September 1, 2022): 402-409. https://doi.org/10.31015/jaefs.2022.3.9.

JAMA

1.Ateş MB, Hatipoğlu D. Effect of nigella sativa oil on bisphenol a-induced hepatotoxicity in wistar albino rats: histopathological and biochemical investigation. int. j. agric. environ. food sci. 2022;6:402–409.

MLA

Ateş, Mehmet Burak, and Durmuş Hatipoğlu. “Effect of Nigella Sativa Oil on Bisphenol A-Induced Hepatotoxicity in Wistar Albino Rats: Histopathological and Biochemical Investigation”. International Journal of Agriculture Environment and Food Sciences, vol. 6, no. 3, Sept. 2022, pp. 402-9, doi:10.31015/jaefs.2022.3.9.

Vancouver

1.Mehmet Burak Ateş, Durmuş Hatipoğlu. Effect of nigella sativa oil on bisphenol a-induced hepatotoxicity in wistar albino rats: histopathological and biochemical investigation. int. j. agric. environ. food sci. 2022 Sep. 1;6(3):402-9. doi:10.31015/jaefs.2022.3.9

Cited By

Quaercetin Improves Renal Functional Disorder and Dyslipidemia Caused by Acute Cadmium Exposure

Manas Journal of Agriculture Veterinary and Life Sciences

https://doi.org/10.53518/mjavl.1196166

Potential of Secondary Metabolite Compounds of Black Cumin Seeds (Nigella sativa L.) as Therapeutic Agents for Chronic Diseases

Drug Design, Development and Therapy

https://doi.org/10.2147/DDDT.S554704

Abstracting & Indexing Services

Google Scholar | Crossref DOI Registry

All content published in this journal is licensed under the Creative Commons Attribution 4.0 International License (CC BY 4.0).
Authors retain copyright of their work and grant the journal a non-exclusive right to publish, reproduce, and distribute the articles within an open-access framework.

Web: dergipark.org.tr/jaefs E-mail: editorialoffice@jaefs.com Phone / WhatsApp: +90 850 309 59 27

Effect of nigella sativa oil on bisphenol a-induced hepatotoxicity in wistar albino rats: histopathological and biochemical investigation

Abstract

Keywords

Abstract

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

Cite

Cited By

Quaercetin Improves Renal Functional Disorder and Dyslipidemia Caused by Acute Cadmium Exposure

Potential of Secondary Metabolite Compounds of Black Cumin Seeds (Nigella sativa L.) as Therapeutic Agents for Chronic Diseases

Abstracting & Indexing Services

© International Journal of Agriculture, Environment and Food Sciences