Investigation of viruses and phytoplasma infections in tomato plantations in Bilecik province, Türkiye

Filiz Randa Zelyüt; Ali Karanfil; Adyatma Irawan Santosa

doi:10.31015/jaefs.2024.4.7

EN

Investigation of viruses and phytoplasma infections in tomato plantations in Bilecik province, Türkiye

Abstract

In this study, the status of infections caused by viruses and phytoplasmas in tomato production areas of Bilecik province was determined using conventional molecular methods. During the 2022 tomato production season, 93 plants exhibiting symptoms such as mosaic, leaf and fruit deformations, flower anomalies, and necrotic spots were collected. Viral agents such as tomato spotted wilt virus (TSWV), cucumber mosaic virus (CMV), southern tomato virus (STV), tobamoviruses, and potyviruses were screened by RT-PCR, while phytoplasmas were detected by nested-PCR. Single, double, and triple infections were detected in 50 of the 93 plants. 17, 5, and 21 plants were infected by a single pathogen for TSWV, STV, and phytoplasmas, respectively. 1, 3, and 2 plants were infected by two pathogens for STV+TSWV, STV+phytoplasma, and TSWV+phytoplasma, respectively. Only one plant detected a triple infection caused by STV, TSWV, and phytoplasmas. The phytoplasma genetic group was determined as 16Sr XII-A by PCR RFLP in-silico and in-vitro methods. Sequencing studies revealed that TSWV had high nucleotide sequence similarity with other Türkiye isolates for the NSs partial gene and STV entire CP gene region. For phytoplasmas, sequencing studies showed that the obtained tomato strains overlapped one-to-one with stolbur strains. Phylogenetic analyses applied with global isolates for TSWV NSs and STV CP gene regions showed the existence of 2 main groups (Clade I and Clade II). TSWV and STV isolates obtained from this study clustered in large main branches (Clade I).

Keywords

Bilecik, Tomato, Virus, Phytoplasma, PCR, Sequencing

Supporting Institution

Bilecik Şeyh Edebali University, The Scientific Research Coordination Unit

Project Number

2022-01.BŞEÜ.06-0

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Details

Primary Language

English

Subjects

Plant Pathology

Journal Section

Research Article

Authors

Filiz Randa Zelyüt ^*
0000-0002-1366-4389
Türkiye

Ali Karanfil
0000-0002-4503-6344
Türkiye

Adyatma Irawan Santosa
0000-0002-2826-5444
Indonesia

Early Pub Date

December 9, 2024

Publication Date

December 28, 2024

Submission Date

September 9, 2024

Acceptance Date

November 1, 2024

Published in Issue

Year 2024 Volume: 8 Number: 4

DOI

https://doi.org/10.31015/jaefs.2024.4.7

IZ

https://izlik.org/JA99SU62NW

APA

Randa Zelyüt, F., Karanfil, A., & Santosa, A. I. (2024). Investigation of viruses and phytoplasma infections in tomato plantations in Bilecik province, Türkiye. International Journal of Agriculture Environment and Food Sciences, 8(4), 786-796. https://doi.org/10.31015/jaefs.2024.4.7

AMA

1.Randa Zelyüt F, Karanfil A, Santosa AI. Investigation of viruses and phytoplasma infections in tomato plantations in Bilecik province, Türkiye. int. j. agric. environ. food sci. 2024;8(4):786-796. doi:10.31015/jaefs.2024.4.7

Chicago

Randa Zelyüt, Filiz, Ali Karanfil, and Adyatma Irawan Santosa. 2024. “Investigation of Viruses and Phytoplasma Infections in Tomato Plantations in Bilecik Province, Türkiye”. International Journal of Agriculture Environment and Food Sciences 8 (4): 786-96. https://doi.org/10.31015/jaefs.2024.4.7.

EndNote

Randa Zelyüt F, Karanfil A, Santosa AI (December 1, 2024) Investigation of viruses and phytoplasma infections in tomato plantations in Bilecik province, Türkiye. International Journal of Agriculture Environment and Food Sciences 8 4 786–796.

IEEE

[1]F. Randa Zelyüt, A. Karanfil, and A. I. Santosa, “Investigation of viruses and phytoplasma infections in tomato plantations in Bilecik province, Türkiye”, int. j. agric. environ. food sci., vol. 8, no. 4, pp. 786–796, Dec. 2024, doi: 10.31015/jaefs.2024.4.7.

ISNAD

Randa Zelyüt, Filiz - Karanfil, Ali - Santosa, Adyatma Irawan. “Investigation of Viruses and Phytoplasma Infections in Tomato Plantations in Bilecik Province, Türkiye”. International Journal of Agriculture Environment and Food Sciences 8/4 (December 1, 2024): 786-796. https://doi.org/10.31015/jaefs.2024.4.7.

JAMA

1.Randa Zelyüt F, Karanfil A, Santosa AI. Investigation of viruses and phytoplasma infections in tomato plantations in Bilecik province, Türkiye. int. j. agric. environ. food sci. 2024;8:786–796.

MLA

Randa Zelyüt, Filiz, et al. “Investigation of Viruses and Phytoplasma Infections in Tomato Plantations in Bilecik Province, Türkiye”. International Journal of Agriculture Environment and Food Sciences, vol. 8, no. 4, Dec. 2024, pp. 786-9, doi:10.31015/jaefs.2024.4.7.

Vancouver

1.Filiz Randa Zelyüt, Ali Karanfil, Adyatma Irawan Santosa. Investigation of viruses and phytoplasma infections in tomato plantations in Bilecik province, Türkiye. int. j. agric. environ. food sci. 2024 Dec. 1;8(4):786-9. doi:10.31015/jaefs.2024.4.7

Cited By

Determination of Viral Diseases in Tomato and Pepper Production Areas of Kırşehir Province, Türkiye

Tekirdağ Ziraat Fakültesi Dergisi

https://doi.org/10.33462/jotaf.1655541

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Investigation of viruses and phytoplasma infections in tomato plantations in Bilecik province, Türkiye

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Determination of Viral Diseases in Tomato and Pepper Production Areas of Kırşehir Province, Türkiye

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