The efficacy of Serratia nematodiphila and Neem Azal T/S on Macrosiphum rosae: new approaches in biological control

Year 2024, Volume: 8 Issue: 4, 884 - 893, 28.12.2024

Halil Dilmen , Utku Şanver , Mehmet Kaplan , Merve Doğaç

https://doi.org/10.31015/jaefs.2024.4.18

Abstract

The rose aphid, Macrosiphum rosae (L.) (Hemiptera: Aphididae), is one of the most common pests of rose plants. This study evaluated the effects of four different doses of Neem Azal-T/S, containing the active ingredient Azadirachtin A, and a dose of Serratia nematodiphila (1x108 cfu/ml) on M. rosae over 72 hours. The experiment was conducted in a climate chamber under controlled conditions (25±1 °C, 60±5% relative humidity, and a 16:8 light-dark photoperiod). The results showed that Neem Azal-T/S led to mortality rates of 12.5%, 17.5%, 60%, and 77.5%, respectively, while S. nematodiphila resulted in a 78% mortality rate after 72 hours. In the control group, mortality was 0.75%, while mortality rates for the treatment groups were 1.25 (Neem_1), 1.75 (Neem_2), 6.00 (Neem_3), 7.00 (Neem_4), and 8.25 (S. nematodiphila). Statistical analyses showed significant differences between all treatment groups and the control. In conclusion, this study demonstrated that both Neem Azal-T/S and S. nematodiphila significantly increased mortality rates in M. rosae compared with the control. Additionally, this study is the first record of the presence of S. nematodiphila in Türkiye and the first information on its entomopathogenic effect on M. rosae in the worldwide, supporting the potential of biological methods in managing rose aphids and emphasizing the importance of biological control strategies in agricultural pest management.

Keywords

Biocontrol , Entomopathogenic bacteria , Natural insecticides , Insecticidal activity

References

• Abbott, W. S. (1925). A method of computing the effectiveness of an insecticide. Journal of economic entomology, 18(2), 265-267.
• Aggarwal, C., Paul, S., Tripathi, V., Paul, B., & Khan, M. A. (2017). Characterization of putative virulence factors of Serratia marcescens strain SEN for pathogenesis in Spodoptera litura. Journal of invertebrate pathology, 143, 115-123. https://doi.org/10.1016/j.jip.2016.12.004
• Alengebawy, A., Abdelkhalek, S. T., Qureshi, S. R., & Wang, M.-Q. (2021). Heavy metals and pesticides toxicity in agricultural soil and plants: Ecological risks and human health implications. Toxics, 9(3), 42. https://doi.org/10.3390/toxics9030042
• Bartelsmeier, I., Kilian, M., & Dicke, M. (2022). Effects of NeemAzal-T/S on different developmental stages of rose aphid, Macrosiphum rosae. Entomologia Experimentalis et Applicata, 170(3), 245-259. https://doi.org/10.1111/eea.13141
• Bel, Y., Ferré, J., & Hernández-Martínez, P. (2020). Bacillus thuringiensis toxins: functional characterization and mechanism of action. Toxins, 12(12), 785. https://doi.org/10.3390/toxins12120785
• Cheng, J., & Haas, M. (1990). Frequent Mutations in the p53 Tumor Suppressor Gene in Human Leukemia T-Cell Lines. Molecular and Cellular Biology, 10(10), 5502-5509. https://doi.org/10.1128/mcb.10.10.5502-5509.1990
• Chi, H. (2024). Computer program for the probit analysis. http://140.120.197.173/Ecology/Dowland/probit-MSChart.rar (accessed 10 September 2024).
• de Mendiburu, F., & de Mendiburu, M. F. (2019). Package ‘agricolae’. R Package, version, 1(3), 1143-1149.
• Giri, A. V., Anandkumar, N., Muthukumaran, G., & Pennathur, G. (2004). A novel medium for the enhanced cell growth and production of prodigiosin from Serratia marcescens isolated from soil. BMC Microbiology, 4(1), 11. https://doi.org/10.1186/1471-2180-4-11
• Golizadeh, A., Jafari-Behi, V., Razmjou, J., Naseri, B., & Hassanpour, M. (2017). Population Growth Parameters of Rose Aphid, Macrosiphum rosae (Hemiptera: Aphididae) on Different Rose Cultivars. Neotropical Entomology, 46(1), 100-106. https://doi.org/10.1007/s13744-016-0428-4
• Hejazi, A., & Falkiner, F. R. (1997). Serratia marcescens. Journal of Medical Microbiology, 46(11), 903-912. https://doi.org/10.1099/00222615-46-11-903
• Hu, W., Zheng, R., Liao, Y., Kuang, F., Yang, Z., Chen, T., & Zhang, N. (2021). Evaluating the biological potential of prodigiosin from Serratia marcescens KH-001 against Asian citrus psyllid. Journal of Economic Entomology, 114(3), 1219-1225. https://doi.org/10.1093/jee/toab041
• Inglis, G. D., & Lawrence, A. M. (2001). Effects of Serratia marcescens on the F1 generation of laboratory-reared Heliothis virescens (Lepidoptera: Noctuidae). Journal of Economic Entomology, 94(2), 362-366. https://doi.org/10.1603/0022-0493-94.2.362
• Jackson, T. A., Boucias, D. G., & Thaler, J.-O. (2001). Pathobiology of amber disease, caused by Serratia spp., in the New Zealand grass grub, Costelytra zealandica. Journal of invertebrate pathology, 78(4), 232-243. https://doi.org/10.1006/jipa.2002.5078
• Jiang, H., Dong, H., Zhang, G., Yu, B., Chapman, L. R., & Fields, M. W. (2006). Microbial diversity in water and sediment of Lake Chaka, an athalassohaline lake in northwestern China. Applied and environmental microbiology, 72(6), 3832-3845. https://doi.org/10.1128/AEM.02869-05
• Jiang, H., Wang, J., Song, L. I., Cao, X., Yao, X. I., Tang, F., & Yue, Y. (2018). Chemical composition of an insecticidal extract from Robinia pseudacacia L. seeds and it’s efficacy against aphids in oilseed rape. Crop protection, 104, 1-6. https://doi.org/10.1016/j.cropro.2017.10.004
• Kim, Y.-G., Kim, K.-S., Seo, J., Shrestha, S., Kim, H.-H., Nalini, M., & Yi, Y.-K. (2009). Identification of an entomopathogenic bacterium, Serratia sp. ANU101, and its hemolytic activity. Journal of microbiology and biotechnology, 19(3), 314-322. https://doi.org/10.4014/jmb.0806.360
• Konecka, E., Mokracka, J., Krzymińska, S., & Kaznowski, A. (2019). Evaluation of The Pathogenic Potential of Insecticidal Serratia marcescens Strains to Humans. Polish Journal of Microbiology, 68(2), 185-191. https://doi.org/10.33073/pjm-2019-018
• Lee, J., & Lee, D.-W. (2022). Insecticidal serralysin of Serratia marcescens is detoxified in M3 midgut region of Riptortus pedestris. Frontiers in Microbiology, 13, 913113. https://doi.org/10.3389/fmicb.2022.913113
• Li, J., Kang, Z., Yu, H., Feng, Y., Zhang, X., Zhao, Y., Dong, L., Zhang, L., Dong, J., Li, Y., & Ma, S. (2023). Potent insecticidal activity of Eleocharis dulcis (Burm. F.) Trin peel extract and its main components against aphids. Pest Management Science, 79(4), 1295-1304. https://doi.org/10.1002/ps.7282
• Lin, D.-J., Zhou, J., Ali, A., Fu, H., Gao, S., Jin, L., & Fang, Y. (2024). Biocontrol efficiency and characterization of insecticidal protein from sugarcane endophytic Serratia marcescens (SM) against oriental armyworm Mythimna separata (Walker). International Journal of Biological Macromolecules, 262, 129978. https://doi.org/10.1016/j.ijbiomac.2024.129978
• Manzano-Marín, A., Lamelas, A., Moya, A., & Latorre, A. (2012). Comparative Genomics of Serratia spp.: Two Paths towards Endosymbiotic Life. Plos One 7(10), e47274. https://doi.org/10.1371/journal.pone.0047274
• Mohapatra, S., Gogoi, I., Bhattacharyya, B., Nath, P. D., & Neog, B. (2021). Efficacy of some indigenous products against cowpea aphid, Aphis craccivora Koch. Indian Journal of Traditional Knowledge (IJTK), 20(3), 822-826.
• Monreal, J., & Reese, E. T. (1969). The chitinase of Serratia marcescens. Canadian Journal of Microbiology, 15(7), 689-696. https://doi.org/10.1139/m69-122
• Muhammad, U., Khattak, T. N., Rahman, H., Daud, M. K., Murad, W., & Azizullah, A. (2018). Effects of Neem (Azadirachta indica) seed and Turmeric (Curcuma longa) rhizome extracts on aphids control, plant growth and yield in okra. Journal of Applied Botany and Food Quality, 91(1), 194-201. https://doi.org/ 10.5073/JABFQ.2018.091.026
• Ngegba, P. M., Cui, G., Khalid, M. Z., & Zhong, G. (2022). Use of botanical pesticides in agriculture as an alternative to synthetic pesticides. Agriculture, 12(5), 600. https://doi.org/10.3390/agriculture12050600
• Niu, H., Sun, Y., Zhang, Z., Zhao, D., Wang, N., Wang, L., & Guo, H. (2022). The endophytic bacterial entomopathogen Serratia marcescens promotes plant growth and improves resistance against Nilaparvata lugens in rice. Microbiological Research, 256, 126956. https://doi.org/10.1016/j.micres.2021.126956
• Nuñez-Valdez, M. E., Calderón, M. A., Aranda, E., Hernández, L., Ramírez-Gama, R. M., Lina, L., Rodríguez-Segura, Z., Gutiérrez, M. D. C., & Villalobos, F. J. (2008). Identification of a Putative Mexican Strain of Serratia entomophila Pathogenic against Root-Damaging Larvae of Scarabaeidae (Coleoptera). Applied and Environmental Microbiology, 74(3), 802-810. https://doi.org/10.1128/AEM.01074-07
• NW, S. (2001). I. Initial identification of common genera. Laboratory guide for identification of plant pathogenic bacteria. https://cir.nii.ac.jp/crid/1572543024776044800
• Pathania, M., Kaur, J., & Singh, B. (2023). Efficacy of Neem Based Formulations against Wheat Aphids under Semi-Arid Irrigated Conditions in South-West Punjab. Indian Journal of Entomology, 1069-1072. https://doi.org/10.55446/IJE.2021.134
• Patil, C. D., Patil, S. V., Salunke, B. K., & Salunkhe, R. B. (2012). Insecticidal potency of bacterial species Bacillus thuringiensis SV2 and Serratia nematodiphila SV6 against larvae of mosquito species Aedes aegypti, Anopheles stephensi, and Culex quinquefasciatus. Parasitology Research, 110(5), 1841-1847. https://doi.org/10.1007/s00436-011-2708-6
• Santos, T. M. dos, Costa, N. P., Torres, A. L., & Boiça Júnior, A. L. (2004). Effect of neem extract on the cotton aphid. Pesquisa Agropecuária Brasileira, 39, 1071-1076. https://doi.org/10.1590/S0100-204X2004001100003
• Sarkhandia, S., Devi, M., Sharma, G., Mahajan, R., Chadha, P., Saini, H. S., & Kaur, S. (2023). Larvicidal, growth inhibitory and biochemical effects of soil bacterium, Pseudomonas sp. EN4 against Spodoptera litura (Fab.) (Lepidoptera: Noctuidae). BMC Microbiology, 23(1), 95. https://doi.org/10.1186/s12866-023-02841-w
• Secil, E. S., Sevim, A., Demirbag, Z., & Demir, I. (2012). Isolation, characterization and virulence of bacteria from Ostrinia nubilalis (Lepidoptera: Pyralidae). Biologia, 67(4), 767-776. https://doi.org/10.2478/s11756-012-0070-5
• Shannag, H. S., Capinera, J. L., & Freihat, N. M. (2014). .Efficacy of different neem-based biopesticides against green peach aphid, Myzus persicae (Hemiptera: Aphididae). International Journal of Agricultural Policy and Research 2(2):061-068.
• Stankovic, S., Kostic, M., Kostic, I., & Krnjajic, S. (2020). Practical Approaches to Pest Control: The Use of Natural Compounds. In Pests-Classification, Management and Practical Approaches; IntechOpen: London, UK,
• Steven, B., Hyde, J., LaReau, J. C., & Brackney, D. E. (2021). The axenic and gnotobiotic mosquito: Emerging models for microbiome host interactions. Frontiers in Microbiology, 12, 714222. https://doi.org/10.3389/fmicb.2021.714222
• Suzuki, K., Suzuki, M., Taiyoji, M., Nikaidou, N., & Watanabe, T. (1998). Chitin Binding Protein (CBP21) in the Culture Supernatant of Serratia marcescens 2170. Bioscience, Biotechnology, and Biochemistry, 62(1), 128-135. https://doi.org/10.1271/bbb.62.128
• Wang Lei, W. L., Zhang Zhuo, Z. Z., Ou XiaoKun, O. X., Zhang JinFeng, Z. J., Jing YueBo, J. Y., Zhang ZhiYing, Z. Z., Cao JianXin, C. J., Li YongPeng, L. Y., Ma SaiYu, M. S., & Li RongBo, L. R. (2010). Preliminary researches on the aphides bio-control with bacteria. Guangxi Agricultural Sciences, 41(3):226-230
• Yang, Q., Gill, A., Robinson, K. L., Umina, P. A., Ross, P. A., Zhan, D., Brown, C., Bell, N., MacMahon, A., & Hoffmann, A. A. (2023). A diversity of endosymbionts across Australian aphids and their persistence in aphid cultures. Environmental Microbiology, 25(10), 1988-2001. https://doi.org/10.1111/1462-2920.16432