Characterization of local Besni pepper (Capsicum annuum L.) genotypes with SRAP and ISSR markers

Mirac Şahin; Halit Yetişir; Hasan Pinar; Akife Dalda Şekerci

EN

Characterization of local Besni pepper (Capsicum annuum L.) genotypes with SRAP and ISSR markers

Abstract

Türkiye has great plant diversity due to its geographical location and suitable climatic and soil conditions. This genetic richness will be meaningful with the studies to be done on it. Characterization studies carried out on local varieties are important in determining the characteristics of the variety and thus including those with superior characteristics in breeding studies. This study aimed to characterize the Besni Pepper genotypes a local variety with molecular (DNA) markers. In the study, 29 pepper genotypes were screened with 10 ISSR and four SRAP primers, and 62 scorable bands were produced. While the polymorphism rate was determined as 76% (47/62), the average number of polymorphic fragments for each primer was calculated as 3.3. According to the created dendrogram, 29 pepper genotypes formed 2 main groups, and these groups were divided into subgroups. The genetic similarity rate was found to be between 0.68-0.95. While the B1 and C3 genotypes were the most distant from each other, the G2 and G3 genotypes were the closest to each other. The study shows that even pepper genotypes from a narrow area have about 32% variation that can be used in future breeding studies. This variation is due to both the fertilization biology (%3-30 cross-pollination) of pepper and seed mobility between farmers. After selection studies for yield, quality, and stress conditions, genotypes can be included in breeding programs.

Keywords

Capsicum annum, Molecular characterization, SRAP, ISSR, Polymorphism

Supporting Institution

This study was financaly supported by Research Projects Coordinating Office (BAP) of Erciyes University with project code FYL-2021-10960.

Project Number

FYL-2021-10960

Ethical Statement

Peer-review Externally peer-reviewed. Competing Interest All authors have no conflicts of interest to disclose.

Thanks

The authors would like to thank Erciyes University Scientific Research Coordination Unit (BAP) and Erciyes University Agricultural Research and Application Center (ERUTAM) for their in-kind and financial support.

References

Adalı, S., (2017). Molecular characterization of some advanced lines of Maraş pepper. Kahramanmaraş Sütçü İmam University, Institute of Science, Department of Agricultural Biotechnology. Kahramanmaraş.
Ahmed, S.M., (2013). Inter-simple sequence repeat (ISSR) markers in the evaluation of genetic polymorphism of Egyptian Capsicum annuum L. hybrids. African Journal of Biotechnology 12 (7): 665-669.
Andrews, J., (1999). The Pepper Trail, History and Recipes from Around the World, University of North Texas Pres, Denton, TX, USA.
Barboza, G.E., Carrizo García, C., Leiva González, S., Scaldaferro, M., Reyes, X., (2019). Four new species of Capsicum (Solanaceae) from the tropical Andes and an update on the phylogeny of the genus. PLoS One 14(1): e0209792. https://doi.org/10.1371/journal.pone.0209792
Barboza, G.E., García. C.C., Bianchetti Lde, B., Romero, M.V., Scaldaferro, M., (2022). Monograph of wild and cultivated chili peppers (Capsicum L., Solanaceae). PhytoKeys 200:1–423. https://doi.org/10.3897/phytokeys.200.71667
Bosland, P.W., (2000). Sources of curly top virus resistance in Capsicum. HortScience 35: 1321- 1322.
Bozokalfa, M.K., Aşcıoğlu, T.K., Eşiyok, D., (2017). Determination of genetic diversity of pepper genotypes using SRAP markers. Anatolian Journal of Agricultural Sciences 32(3): 321-329. DOI:10.7161/omuanajas.284511
Cesur, E., Karakurt, Y., Güvercin, D., (2020). Molecular characterization of pepper (Capsicum annum L.) genotypes using SSR markers. Ege Üniv Ziraat Fak Derg 57 (2):185-189 DOI: 10.20289/zfdergi.614237
Crosby, K., Pike, L., Jifon, J., Yoo, K., (2005). Breeding vegetables for optimum levels of phytochemicals. Proceedings of FAV2005, Quebec City, Canada.
Doyle, J., (1991). DNA Protocols for Plants. In: Hewitt, G.M., Johnston, A.W.B., Young, J.P.W. (eds) Molecular Techniques in Taxonomy. NATO ASI Series, vol 57. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-83962-7_18

Ekbiç, E., Okay, C.Ö., (2024). Assessment of elite pepper breeding lines using molecular markers. Plant Biotechnol Rep 18: 515–524 https://doi.org/10.1007/s11816-024-00911-7
FAO, (2023). https://www.fao.org/faostat/en/#data/QCL (Accessed: [27/ 03 / 2025]).
Fattori, V., Hohmann, M.S., Rossaneis, A.C., et al., (2016). Capsaicin: Current understanding of its mechanisms and therapy of pain and other pre-clinical and clinical uses. Molecules 21(7): 844. doi:10.3390/molecules21070844
Figdore, S.S., Kennard, W.C., Song, K.M., Slocum, M.K., Osborn, T.C., (1988). Assessment of the degree of restriction length polymorphism in Brassica. Theoretical and Applied Genetics 75: 833–940. https://doi.org/10.1007/BF00258042
García-Gaytán, V., Gómez-Merino, F.C., Trejo-Téllez, L.L., Baca-CastilloG, A., García-Morales, S., (2017). The chilhuacle chili (Capsicum annuum L.) in Mexico: Description of the variety, its cultivation, and uses. International Journal of Agronomy. e5641680. doi:10.1155/2017/5641680. ISSN 1687-8159
Geleta, L.F., Labuschagne, M.T., Viljoen, C.D., (2005). Genetic variability in pepper (Capsicum annuum L.) estimated by morphological data and amplified fragment length polymorphism markers. Biodiver Conserv 14: 2361-2375. https://doi.org/10.1007/s10531-004-1669-9
Hazarika, R., Neog, B., (2014). Evaluation of genetic diversity in Bhut jolokia (Capsicum chinense Jacq) accessions using ISSR marker. International Journal of Basic & Applied Science Research 1 (1): 72-79.
Heiser, C.B., Smith, P.G., (1953). The cultivated capsicum peppers. Economic Botany 1953, 7:214–227. doi: 10.1007/BF02984948.
Hulse-Kemp, A.M., Ashrafi, H., Plieske, J., Lemm, J., Stoffel, K., Hill, T., Luerssen, H., Pethiyagoda, C.L., Lawley, C.T., Ganal, M.W., Deynze, A.V., (2016). A HapMap leads to a Capsicum annuum SNP infinium array: a new tool for pepper breeding. Horticulture Research 3: 16036. 10.1038/hortres.2016.36
Ibarra-Torres, P., Valadez-Moctezuma, E., Perez-Grajales, M., Rodríguez-Campos, R., Jaramillo-Flores, M. E., (2014). Inter- and intraspecific differentiation of Capsicum annuum and Capsicum pubescens using ISSR and SSR markers. Scientia Horticulturae, 181, 137-146.
Ifas, (2021). https://bmp.ifas.ufl.edu/ (Accessed: [27/ 03 / 2021]).
Jiang, G.L., (2013). Molecular markers and marker-assisted breeding in plants. In: Andersen SB, editor. Plant breeding from laboratories to fields. Rijeka: InTech: 45–83.
Karataş, A., Büyükdinç, D.T., İpek, A., Yağcıoğlu, M., Sönmez, K., Ellialtıoğlu, Ş.Ş., (2017). Morphological and molecular characterization studies on beans in Turkey. Turkish Journal of Scientific Reviews 10(1): 16-27.
Lijun, O., Xuexiao, Z., (2012). Inter Simple Sequence Repeat analysis of genetic diversity of five cultivated pepper species. African Journal of Biotechnology 11 (4): l752757. 10.5897/AJB10.2551
López-Espinoza, S.T., Latournerie-Moreno, L., Castañón-Nájera, G., Ruiz-Sánchez, E., Gómez-Leyva, JF, Andueza-Noh, R.H., Mijangos-Cortés, J.O., (2018). Genetic diversity of habanero pepper (Capsicum chinense Jacq.) using ISSR. Rev. Fitotec Mex 41:3 227-236. https://doi.org/10.35196/rfm.2018.3.227-236.
Okay, C.Ö., (2019). Molecular characterization of elite pepper breeding lines for genetics and resistance to some virus diseases. Ordu University, Institute of Science, Department of Horticulture, Ordu.
Olatunji, T.L., Afolayan, A.J., (2019). Evaluation of genetic relationship among varieties of Capsicum annuum L. and Capsicum frutescens L. in West Africa using ISSR markers, Heliyon, 5: 01700. https://doi.org/10.1016/j.heliyon.2019.e01700
Pınar, H., Coşkun, Ö.F., Uysal, E., Gülşen, O., Yetişir, H., (2017). Characterization of local chili pepper genotypes with ISSR markers. Academic Journal of Agriculture 6: 145-150.
Rana, M., Sharma, R., Sharma, P., Bhardwaj, S., Sharma, M., (2014). Estimation of genetic diversity in Capsicum annuum L. germplasm using PCR-based molecular markers. National Academy Science Letters 37:3, 295–301. https://doi.org/10.1007/s40009-014-0236-5
Rego, E.R., Nascimento, M.F., Nascimento, N.F.F., Santos, R.M.C., Fortunato, F.L.G., Rego, M.M., (2012). Testing methods for producing self-pollinated fruits in ornamental peppers. Horticultura Brasileira, 30: 669- 672. 10.1590/S0102-05362012000400017
Roy, A., (2016) Bhut Jolokia (Capsicum chinense Jaqc): A Review. International Journal of Pharmaceutical Sciences and Research,7(3): 882-889. 10.13040/IJPSR.0975-8232.7(3).882-89
Sahin, M., Yetişir, H., Pinar, H., (2022). Morphological characterization of some Besni pepper (Capsicum annuum L.) genotypes in Kayseri conditions. International Journal of Agriculture Environment and Food Sciences, 6(1): 152-164. https://doi.org/10.31015/jaefs.2022.1.20
Samos, A., Kundt, A., (1984). The paprika. Kultura Hungarian foreign trade company and academica kiado, Budapest
Şeker, A., (2018). Molecular Characterization of Some Pepper (Capsicum annuum L.) Varieties with SSR Markers. Master thesis, AU, Institute of Science, Department of Horticulture, Ankara.
Singh, A.K., Singh, B., Gupta, R., (2011). Performance of sweet pepper (Capsicum annum) varieties and economics under protected and open field conditions in Uttarakhand. Indian Journal of Agricultural Sciences, 81.
Swamy, K.R.M., (2023). Origin, distribution, taxonomy, botanical description, genetic diversity and breeding of capsicum (Capsicum annuum L.), International Journal of Development Research, 13, (03), 61956-61977. https://doi.org/10.37118/ijdr.26395.03.2023
Tan, A., (1992). Türkiye’de bitkisel çeşitlilik ve bitki genetik kaynakları. Anadolu J. of AARI, 2(2): 50-54.
Tan, A., İnal, A., (2003). Ege Tarımsal Araştırma Enstitüsü bitki genetik kaynakları çalışmaları, Ege Tarımsal Araştırma Enstitüsü Müdürlüğü Yayın No:112, 13, İzmir.
Tatar, M., (2022). Morphological and Molecular Characterization of Gaziantep Local Pepper Genotypes and Possibilities of Obtaining Double Haploid (Dh) Lines. Doctoral Thesis, Van Yuzuncu Yil University, Institute of Science Van.
TUIK, (2024). https://biruni.tuik.gov.tr/medas/?locale=tr (Accessed: [27/ 03 / 2025]).
Uzun, A., Yesiloglu, T., Aka-Kacar, Y., Tuzcu, O., Gulsen, O., (2009). Genetic diversity and relationships within Citrus and related genera based on sequence-related amplified polymorphism markers (SRAPs). Scientia Horticulturae 2: 306-312. https://doi.org/10.1016/j.scienta.2009.02.018
Vidhi, J., (2023). Capsicum: Origin, Flower Structure and Varieties India. https://www.biologydiscussion.com/vegetable-breeding/ (Accessed: [27/ 03 / 2025]).
Vural, H., Eşiyok, D., Duman, İ., (2000). Kültür Sebzeleri (Vegetable Production), Ege Ün. Ziraat Fak.Bahçe Bitkileri Bölümü, Bornova, İzmir.
Wahyuni, Y., Ballester, A.R., Sudarmonowati, E., Bino, R.J., Bovy, A.G., (2011). Metabolite biodiversity in pepper (Capsicum) fruits of thirty-two diverse accessions: variation in health-related compounds and implications for breeding. Phytochemistry72: 1358–70. 10.1016/j.phytochem.2011.03.016
Yang, R., Kong, J., Wu, X., Deng, Z., Chen, Q., Liu, W., (2005). Application of ISSR markers in genetic polymorphism of Capsicum frutescens L. Journal of Shanghai University (Natural Science Edition) 4-20.
Zhang, L., Li, P., Zhang, X., Jinfeng, L., (2023). Two floral forms in the same species distyly. Planta 258:72 https://doi.org/10.1007/s00425-023-04229-6
Zhigila, D.A., AbdulRahaman, A.A., Kolawole, O.S., Oladele, F.A., (2014). Fruit morphology as taxonomic features in five varieties of Capsicum annuum L. Solanaceae". Journal of Botany. e540868. doi:10.1155/2014/540868. ISSN 2090-0120

Details

Primary Language

English

Subjects

Non-Genetically Modified Uses of Biotechnology

Journal Section

Research Article

Authors

Mirac Şahin
0000-0002-7699-6380
Türkiye

Halit Yetişir ^*
0000-0001-6955-9513
Türkiye

Hasan Pinar
0000-0002-0811-8228
Türkiye

Akife Dalda Şekerci
0000-0001-8554-6501
Türkiye

Publication Date

June 26, 2025

Submission Date

February 27, 2025

Acceptance Date

April 14, 2025

Published in Issue

Year 2025 Volume: 9 Number: 2

IZ

https://izlik.org/JA57XA88AA

APA

Şahin, M., Yetişir, H., Pinar, H., & Dalda Şekerci, A. (2025). Characterization of local Besni pepper (Capsicum annuum L.) genotypes with SRAP and ISSR markers. International Journal of Agriculture Environment and Food Sciences, 9(2), 272-282. https://izlik.org/JA57XA88AA

AMA

1.Şahin M, Yetişir H, Pinar H, Dalda Şekerci A. Characterization of local Besni pepper (Capsicum annuum L.) genotypes with SRAP and ISSR markers. int. j. agric. environ. food sci. 2025;9(2):272-282. https://izlik.org/JA57XA88AA

Chicago

Şahin, Mirac, Halit Yetişir, Hasan Pinar, and Akife Dalda Şekerci. 2025. “Characterization of Local Besni Pepper (Capsicum Annuum L.) Genotypes With SRAP and ISSR Markers”. International Journal of Agriculture Environment and Food Sciences 9 (2): 272-82. https://izlik.org/JA57XA88AA.

EndNote

Şahin M, Yetişir H, Pinar H, Dalda Şekerci A (June 1, 2025) Characterization of local Besni pepper (Capsicum annuum L.) genotypes with SRAP and ISSR markers. International Journal of Agriculture Environment and Food Sciences 9 2 272–282.

IEEE

[1]M. Şahin, H. Yetişir, H. Pinar, and A. Dalda Şekerci, “Characterization of local Besni pepper (Capsicum annuum L.) genotypes with SRAP and ISSR markers”, int. j. agric. environ. food sci., vol. 9, no. 2, pp. 272–282, June 2025, [Online]. Available: https://izlik.org/JA57XA88AA

ISNAD

Şahin, Mirac - Yetişir, Halit - Pinar, Hasan - Dalda Şekerci, Akife. “Characterization of Local Besni Pepper (Capsicum Annuum L.) Genotypes With SRAP and ISSR Markers”. International Journal of Agriculture Environment and Food Sciences 9/2 (June 1, 2025): 272-282. https://izlik.org/JA57XA88AA.

JAMA

1.Şahin M, Yetişir H, Pinar H, Dalda Şekerci A. Characterization of local Besni pepper (Capsicum annuum L.) genotypes with SRAP and ISSR markers. int. j. agric. environ. food sci. 2025;9:272–282.

MLA

Şahin, Mirac, et al. “Characterization of Local Besni Pepper (Capsicum Annuum L.) Genotypes With SRAP and ISSR Markers”. International Journal of Agriculture Environment and Food Sciences, vol. 9, no. 2, June 2025, pp. 272-8, https://izlik.org/JA57XA88AA.

Vancouver

1.Mirac Şahin, Halit Yetişir, Hasan Pinar, Akife Dalda Şekerci. Characterization of local Besni pepper (Capsicum annuum L.) genotypes with SRAP and ISSR markers. int. j. agric. environ. food sci. [Internet]. 2025 Jun. 1;9(2):272-8. Available from: https://izlik.org/JA57XA88AA

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Characterization of local Besni pepper (Capsicum annuum L.) genotypes with SRAP and ISSR markers

Abstract

Characterization of local Besni pepper (Capsicum annuum L.) genotypes with SRAP and ISSR markers

Abstract

Keywords

Supporting Institution

Project Number

Ethical Statement

Thanks

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

IZ

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