SCoT marker-based evaluation of genetic diversity in selected walnut (Juglans regia L.) accessions

Gökhan Ovalıoglu; Yeter Çilesiz

doi:10.31015/2025.2.30

EN

SCoT marker-based evaluation of genetic diversity in selected walnut (Juglans regia L.) accessions

Abstract

This study investigates the genetic diversity among different walnut (Juglans regia L.) genotypes using the SCoT (Start Codon Targeted) marker system. A total of 136 bands were obtained from 15 markers, of which 106 were polymorphic, with an average of 9.07 bands per primer. The SCoT markers yielded a polymorphism rate of 77.9%. In order to interpret the genetic distance among Juglans regia L. genotypes, a UPGMA dendrogram was constructed using MVSP 3.22 software. According to the resulting dendrogram, the lowest similarity (0.593) was observed between samples W6 and W26, while the highest similarity (0.970) was observed between W1 and W4, followed by 0.962 between W4 and W5. Principal Component Analysis (PCA) was also conducted using MVSP 3.22. The PCA results revealed a homogeneous distribution and wide variation. The UPGMA dendrogram and PCA analysis were consistent with each other. The SCoT analyses conducted on walnut genotypes provide highly valuable information for assessing the level of genetic diversity, understanding population structure and selecting superior individuals. Such studies contribute to the conservation of genetic resources and support the development of new cultivars with high yield, disease resistance and strong adaptation to climate change in future breeding programs. Based on this study, SCoT analyses demonstrate that the Sivas province constitutes an important genetic diversity reservoir and that walnut genotypes possess rich genetic variation.

Keywords

Juglans regia L., SCoT marker, Genetic diversity

Supporting Institution

Sivas Science and Technology University Scientific Research Projects Coordination

Project Number

2025-YLTP-TBT-0001

Thanks

This study was produced from the project number 2025-YLTP-TBT-0001 (Determination of genetic diversity in some walnut genotypes (Juglans regia L.) using start codon-targeted (SCoT) markers), which was carried out with the support of Sivas Science and Technology University Scientific Research Projects Coordination. This study was derived from the first author's master's thesis titled "Determination of Genetic Diversity in Some Walnut Genotypes (Juglans regia L.) Using Start Codon Targeted (SCoT) Markers."

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Details

Primary Language

English

Subjects

Food Biotechnology, Agricultural Biotechnology (Other)

Journal Section

Research Article

Authors

Gökhan Ovalıoglu This is me
0009-0004-6235-8435
Türkiye

Yeter Çilesiz ^*
0000-0002-4313-352X
Türkiye

Publication Date

June 26, 2025

Submission Date

April 27, 2025

Acceptance Date

June 7, 2025

Published in Issue

Year 2025 Volume: 9 Number: 2

DOI

https://doi.org/10.31015/2025.2.30

IZ

https://izlik.org/JA72WF85ZS

APA

Ovalıoglu, G., & Çilesiz, Y. (2025). SCoT marker-based evaluation of genetic diversity in selected walnut (Juglans regia L.) accessions. International Journal of Agriculture Environment and Food Sciences, 9(2), 570-576. https://doi.org/10.31015/2025.2.30

AMA

1.Ovalıoglu G, Çilesiz Y. SCoT marker-based evaluation of genetic diversity in selected walnut (Juglans regia L.) accessions. int. j. agric. environ. food sci. 2025;9(2):570-576. doi:10.31015/2025.2.30

Chicago

Ovalıoglu, Gökhan, and Yeter Çilesiz. 2025. “SCoT Marker-Based Evaluation of Genetic Diversity in Selected Walnut (Juglans Regia L.) Accessions”. International Journal of Agriculture Environment and Food Sciences 9 (2): 570-76. https://doi.org/10.31015/2025.2.30.

EndNote

Ovalıoglu G, Çilesiz Y (June 1, 2025) SCoT marker-based evaluation of genetic diversity in selected walnut (Juglans regia L.) accessions. International Journal of Agriculture Environment and Food Sciences 9 2 570–576.

IEEE

[1]G. Ovalıoglu and Y. Çilesiz, “SCoT marker-based evaluation of genetic diversity in selected walnut (Juglans regia L.) accessions”, int. j. agric. environ. food sci., vol. 9, no. 2, pp. 570–576, June 2025, doi: 10.31015/2025.2.30.

ISNAD

Ovalıoglu, Gökhan - Çilesiz, Yeter. “SCoT Marker-Based Evaluation of Genetic Diversity in Selected Walnut (Juglans Regia L.) Accessions”. International Journal of Agriculture Environment and Food Sciences 9/2 (June 1, 2025): 570-576. https://doi.org/10.31015/2025.2.30.

JAMA

1.Ovalıoglu G, Çilesiz Y. SCoT marker-based evaluation of genetic diversity in selected walnut (Juglans regia L.) accessions. int. j. agric. environ. food sci. 2025;9:570–576.

MLA

Ovalıoglu, Gökhan, and Yeter Çilesiz. “SCoT Marker-Based Evaluation of Genetic Diversity in Selected Walnut (Juglans Regia L.) Accessions”. International Journal of Agriculture Environment and Food Sciences, vol. 9, no. 2, June 2025, pp. 570-6, doi:10.31015/2025.2.30.

Vancouver

1.Gökhan Ovalıoglu, Yeter Çilesiz. SCoT marker-based evaluation of genetic diversity in selected walnut (Juglans regia L.) accessions. int. j. agric. environ. food sci. 2025 Jun. 1;9(2):570-6. doi:10.31015/2025.2.30

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International Journal of Agriculture, Environment and Food Sciences

e-ISSN: 2618-5946 | Publisher: Gültekin Özdemir | DOI Prefix: 10.31015

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