Evaluation of Gibberellic Acid Pretreatment in the Alleviation of Salinity-induced Stress in Buckwheat Seeds
Abstract
This study investigated the effects of salinity stress on buckwheat (Fagopyrum esculentum) seed germination and the contribution of different gibberellic acid (GA₃) doses to mitigate this stress. Salinity is one of the major abiotic stress factors that negatively affect crop yield and quality worldwide. Improved seed germination has been achieved through various seed pre-treatment methods. Gibberellic acid is an important plant growth regulator that plays a role in the growth and development processes of many plant species. This research was conducted on the var. Aktas of buckwheat treated with three doses of (0, 5, 10, 20 dS m-1) of NaCl and post-treated with three different GA₃ doses (0, 100, 200 mg L-1). The study analyzed mean germination time, germination percentage, shoot and root length, fresh and dry weight data of the buckwheat seeds. The results revealed significant differences among salinity levels and alleviating effects of gibberellic acid post treatments on NaCl pretreated seedlings. It was observed that seed post-treatment with gibberellic acid positively affected germination parameters at increasing salt concentrations. The best results were observed in seedlings treated with 200 mg L-1 gibberellic acid on non-salt treated seeds under controlled conditions (0 dS m⁻¹, control).These findings suggest that gibberellic acid treatments have a potential to enhance buckwheat germination under salinity stress and could be used commercially.
Keywords
Abiotic stress, Gibberellic acid, Germination, Fagopyrum esculentum
Supporting Institution
Department of Field Crops, Faculty of Agriculture, Ankara University, Ankara
References
- Abdel-Hamid, A.M., & Mohamed, H.I. (2014). The effect of the exogenous gibberellic acid on two salt stressed barley cultivars. European Scientific Journal, 10(6), 228-245.
- Anwar, T., Munwwar, F., Qureshi, H., Siddiqi, E. H., Hanif, A., Anwaar, S.,Gul, S., Waheed, A., Alwahibi, M.S., & Kamal, A. (2023). Synergistic effect of biochar-based compounds from vegetable wastes and gibberellic acid on wheat growth under salinity stress. Scientific Reports, 13(1), 19024.
- Arif, Y., Singh, P., Siddiqui, H., Bajguz, A., & Hayat, S. (2020). Salinity induced physiological and biochemical changes in plants: An omic approach towards salt stress tolerance. Plant Physiology and Biochemistry, 156, 64-77.
- Attia, H., Alamer, K., Algethami, B., Zorrig, W., Hessini, K., Gupta, K., & Gupta, B. (2022). Gibberellic acid interacts with salt stress on germination, growth and polyamine gene expression in fennel (Foeniculum vulgare Mill.) seedlings. Physiology and Molecular Biology of Plants, 28(3), 607-622.
- Chrungoo, N. K. and Chettry, U. (2021). Buckwheat: A critical approach towards assessment of its potential as a super crop. Indian Journal of Genetics and Plant Breeding, 81(01), 1-23.
- Day, S., & Koçak-Şahin, N. (2023). Seed Priming with MgCl2, CaCl2, and ZnCl2 as a Biofortification Based-Approach Induces Changes in Anise Seedlings Emergence. Phyton, (0031-9457), 92(8).
- Day, S., Koçak, N., & Önol, B. (2024). Hemp seed priming via different agents to alleviate temperature stress. Journal of Agricultural Sciences, 30(3), 562-569.
- El Sabagh, A., Islam, M. S., Skalicky, M., Ali Raza, M., Singh, K., Anwar Hossain, M., Hussain, A., Mahboob, W., Iqbal, M.A. Ratnasekera, D., Signhal, D., Signhal, R.K., Ahmed, S., Kumari, A., Wasaya, A., Sytar, O., Brectic, M., Çıg, F., Erman, M., Rahman, M.H., Ullah, N., & Arshad, A. (2021). Salinity stress in wheat (Triticum aestivum L.) in the changing climate: Adaptation and management stpercentagegies. Frontiers in Agronomy, 3, 661932.
- Essa, T.A., Salama, A.M., & El-Tayeb, M.A. (2019). Influence of gibberellic acid on seed germination of Hordeum vulgare under salt stress conditions. Journal of Crop Science, 30(2), 312-320.
- Gueridi, S., Boucelha, L., Abrous-Belbachir, O., & Djebbar, R. (2024). Effects of hormopriming and pretreatment with gibberellic acid on fenugreek (Trigonella foenum graecum L.) seed germination. Acta Botanica Croatica, 83(2), 0-0.
