Effectiveness of Native Trichoderma Isolates Against Gray Mold in Vitis vinifera

Yunus Korkom; Emrecan Altıntaş

doi:10.31015/2025.4.18

Effectiveness of Native Trichoderma Isolates Against Gray Mold in Vitis vinifera

Abstract

Gray mold is a significant fungal disease in grapevine cultivation, causing by Botrytis cinerea. This study aimed to evaluate the antagonistic effect of eight native Trichoderma isolates against B. cinerea. In vitro assessments using dual culture tests demonstrated mycelial growth varying degrees of inhibition. Trichoderma isolates inhibited the mycelial growth of B. cinerea by 68.0-79.4%. Five Trichoderma isolates (Tr91, Tr52, Tr142, Tr117, and Tr93) showed high effect against B. cinerea. In vivo experiments were executed to further assess antifungal efficacy. Trichoderma spore suspension were collected after ten days incubation in potato dextrose agar (PDA). Spore concentration of Trichoderma was adjusted to 1×109 spore/mL. B. cinerea was growthed five days in PDA for acquiring conidial suspension. Conidia suspension of B. cinerea (1×10⁶ conidia/mL) was first sprayed to the leaves and bunches with a custom-built spray tower at 1 atm pressure, followed two hours later by Trichoderma spore suspension (spraying 2 mL per plant tissues). Control groups included B. cinerea only (positive), and a sterile distilled water only (negative). Results in the leaves test showed that Trichoderma sp. Tr52 significantly reduced disease severity of B. cinerea by 51.3% compared to the positive control. Trichoderma sp. Tr52 had an exhibit highest efficacy (50.7%) against gray mold disease in bunches. These findings suggest that native Trichoderma isolates have potential as a biological control against gray mold on grapevine. Future research should focus on evaluating their effectiveness against different fungal pathogens of limiting grapevine cultivation.

Keywords

Trichoderma, Grapevine, Botrytis cinerea, Biological control

Supporting Institution

The Scientific and Technological Research Council of Türkiye (TUBITAK)

Project Number

TUBITAK-Project No. 1919B012327718.

Thanks

We acknowledge the Scientific and Technological Research Council of Türkiye (TUBITAK) for the financial support of this work.

References

Abbott, W.S. (1925). A method of computing the effectiveness of an insecticide.Journal Economic Entomology, 18, 265–267. https://doi.org/10.1093/jee/18.2.265a.
Arici, S.E., & Tuncel, Z.N. (2020). Antifungal activity of useful microorganisms against a phytopathogenic fungus on maize. Emerging Materials Research, 9(3), 743–749. https://doi.org/10.1680/jemmr.20.00121
Avenot, H.F., Quattrini, J., Puckett, R., & Michailides, T.J. (2018). Different levels of resistance to cyprodinil and iprodione and lack of fludioxonil resistance in Botrytis cinerea isolates collected from pistachio, grape, and pomegranate fields in California. Crop Protection, 112, 274–281. https://doi.org/10.1016/j.cropro.2018.06.005
Bi, K., Liang, Y., Mengiste, T., & Sharon, A. (2023). Killing softly: a roadmap of Botrytis cinerea pathogenicity. Trends Plant Science, 28(2), 211–222. https://doi.org/10.1016/j.tplants.2022.08.024
Campia, P., Venturini, G., Moreno‐Sanz, P., Casati, P.L.T.S., & Toffolatti, S.L. (2017). Genetic structure and fungicide sensitivity of Botrytis cinerea populations isolated from grapevine in northern Italy. Plant Pathology, 66(6), 890–899. https://doi.org/10.1111/ppa.12643
Carisse, O., & Van Der Heyden, H. (2025). Knowledge-based integrated management of Botrytis bunch rot of grapevine caused by Botrytis cinerea in a Northern climate. PhytoFrontiers, 5(3), 418–428. https://doi.org/10.1094/PHYTOFR-12-24-0139-R
Ciliberti, N., Fermaud, M., Roudet, J., Languasco, L., & Rossi, V. (2016). Environmental effects on the production of Botrytis cinerea conidia on different media, grape bunch trash, and mature berries. Australian Journal of Grape and Wine Research, 22(2), 262-270. https://doi.org/10.1111/ajgw.12217
Devi, R., Kaur, T., Kour, D., Rana, K.L., Yadav, A., & Yadav, A.N. (2020). Beneficial fungal communities from different habitats and their roles in plant growth promotion and soil health. Microbial Biosystems, 5(1), 21–47. https://doi.org/10.21608/mb.2020.32802.1016
Doni, F., Zain, C.R.C.M., Isahak, A., Fathurrahman, F., Anhar, A., Mohamad, W.N.A.W., ... & Uphoff, N. (2018). A simple, efficient, and farmer-friendly Trichoderma-based biofertilizer evaluated with the SRI Rice Management System. Organic Agriculture, 8(3), 207–223. https://doi.org/10.1007/s13165-017-0185-7
Evren, G., Korkom, Y., Saboori, A., & Cakmak, I. (2025). Exploring the potential of Trichoderma secondary metabolites against Tetranychus urticae (Acari: Tetranychidae). Journal of Invertebrate Pathology, 211, 108299. https://doi.org/10.1016/j.jip.2025.108299

FAOSTAT, (2024). Statistical database. World: Food and Agriculture Organization of the United Nations. http://apps.fao.org/page/collections?subset=agriculture (accessed October, 2025).
Fedele, G., Brischetto, C., González-Domínguez, E., & Rossi, V. (2020). The colonization of grape bunch trash by microorganisms for the biocontrol of Botrytis cinerea as influenced by temperature and humidity. Agronomy, 10(11), 1829. https://doi.org/10.3390/agronomy10111829
Garfinkel, A.R. (2021). The history of Botrytis taxonomy, the rise of phylogenetics, and implications for species recognition. Phytopathology, 111(3), 437–454. https://doi.org/10.1094/PHYTO-06-20-0211-IA
Geng, L., Fu, Y., Peng, X., Yang, Z., Zhang, M., Song, Z., ... & Ahammed, G.J. (2022). Biocontrol potential of Trichoderma harzianum against Botrytis cinerea in tomato plants. Biological Control, 174, 105019. https://doi.org/10.1016/j.biocontrol.2022.105019
González-Martínez, K.I., Vázquez-Garcidueñas, M.S., Herrera-Estrella, A., Fernández-Pavía, S.P., Salgado-Garciglia, R., Larsen, J., ... & Vázquez-Marrufo, G. (2024). Polyphasic characterization of the biocontrol potential of a novel strain of Trichoderma atroviride isolated from central Mexico. Journal of Fungi, 10(11), 758. https://doi.org/10.3390/jof10110758
Harman, G.E., Herrera-Estrella, A.H., Horwitz, B.A., & Lorito, M. (2012). Trichoderma-from basic biology to biotechnology. Microbiology-Reading, 158(1), 1–2. https://doi.org/10.1099/mic.0.056424-0
Jampala, P., Tadikamalla, S., Preethi, M., Ramanujam, S., & Uppuluri, K.B. (2017). Concurrent production of cellulase and xylanase from Trichoderma reesei NCIM 1186: enhancement of production by desirability-based multi-objective method. 3 Biotech, 7(1), 14. https://doi.org/10.1007/s13205-017-0607-y
Jiang, L., Qiu, Y., Dumlao, M.C., Donald, W.A., Steel, C.C., & Schmidtke, L.M. (2023). Detection and prediction of Botrytis cinerea infection levels in wine grapes using volatile analysis. Food Chemistry, 421, 136120. https://doi.org/10.1016/j.foodchem.2023.136120
Kasfi, K., Taheri, P., Jafarpour, B., & Tarighi, S. (2018). Identification of epiphytic yeasts and bacteria with potential for biocontrol of grey mold disease on table grapes caused by Botrytis cinerea. Spanish Journal Agricultural Research, 16(1), 23.
Kaur, J., Goswami, D., & Saraf, M. (2025). Trichoderma’s metabolomic journey: Unveiling bioactive secondary metabolites for sustainable solutions for agriculture and beyond. Indian Journal Microbiology, 1–21. https://doi.org/10.1007/s12088-025-01507-7
Kelly, J., Inglis, D., Dowling, L., & Pickering, G. (2022). Impact of Botrytis cinerea‐infected grapes on quality parameters of red wine made from withered grapes. Australian Journal Grape Wine Research, 28(3), 439–449. https://doi.org/10.1111/ajgw.12545
Kong, Q., Qi, J., An, P., Deng, R., Meng, J., & Ren, X. (2020). Melaleuca alternifolia oil can delay nutrient damage of grapes caused by Aspergillus ochraceus through regulation of key genes and metabolites in metabolic pathways. Postharvest Biology Technology, 164, 111152. https://doi.org/10.1016/j.postharvbio.2020.111152
Korkom, Y. (2022). Determination of the effects of Trichoderma spp., obtained from agricultural areas in Aydin province, on charcoal rot disease in strawberry and plant growth. Ph.D. Thesis, Aydın Adnan Menderes University, Aydın, Türkiye.
Li, F., Ghanizadeh, H., Song, W., Miao, S., Wang, H., Chen, X., ... & Wang, A. (2023). Combined use of Trichoderma harzianum and Clonostachys rosea to manage Botrytis cinerea infection in tomato plants. European Journal of Plant Pathology, 167(4), 637–650. https://doi.org/10.1007/s10658-023-02732-w
Ling, L., Luo, H., Zhao, Y., Yang, C., Cheng, W., & Pang, M. (2023). Fungal pathogens causing postharvest fruit rot of wolfberry and inhibitory effect of 2, 3-butanedione. Frontiers Microbiology, 13, 1068144. https://doi.org/10.3389/fmicb.2022.1068144
Manzar, N., Kashyap, A.S., Goutam, R.S., Rajawat, M.V.S., Sharma, P.K., Sharma, S.K., & Singh, H.V. (2022). Trichoderma: advent of versatile biocontrol agent, its secrets and insights into mechanism of biocontrol potential. Sustainability, 14(19), 12786. https://doi.org/10.3390/su141912786
Martinez, F., Blancard, D., Lecomte, P., Levis, C., Dubos, B., & Fermaud, M. (2003). Phenotypic differences between vacuma and transposa subpopulations of Botrytis cinerea. European Journal of Plant Pathology, 109(5), 479-488. https://doi.org/10.1023/A:1024222206991
Martinez, Y., Ribera, J., Schwarze, F.W., & De France, K. (2023). Biotechnological development of Trichoderma-based formulations for biological control. Applied Microbiology Biotechnology, 107(18), 5595–5612. https://doi.org/10.1007/s00253-023-12687-x
Mishra, S., Lin, Z., Pang, S., Zhang, W., Bhatt, P., & Chen, S. (2021). Recent advanced technologies for the characterization of xenobiotic-degrading microorganisms and microbial communities. Frontiers Bioengineering Biotechnology, 9, 632059. https://doi.org/10.3389/fbioe.2021.632059
Monte, E. (2023). The sophisticated evolution of Trichoderma to control insect pests. Proceedings of the National Academy of Sciences, 120(12), e2301971120. https://doi.org/10.1073/pnas.2301971120
Mundy, D.C., Agnew, R.H., & Wood, P.N. (2012). Grape tendrils as an inoculum source of Botrytis cinerea in vineyards a review. New Zealand Plant Protection, 65, 218-227. https://doi.org/10.30843/nzpp.2012.65.5373
Nawrocka, J., Szymczak, K., Skwarek-Fadecka, M., & Małolepsza, U. (2023). Toward the analysis of volatile organic compounds from tomato plants (Solanum lycopersicum L.) treated with Trichoderma virens or/and Botrytis cinerea. Cells, 12(9), 1271. https://doi.org/10.3390/cells12091271
Orozco-Mosqueda, M.D.C., Kumar, A., Fadiji, A.E., Babalola, O.O., Puopolo, G., & Santoyo, G. (2023). Agroecological management of the grey mould fungus Botrytis cinerea by plant growth-promoting bacteria. Plants, 12(3), 637. https://doi.org/10.3390/plants12030637
Petrasch, S., Knapp, S.J., Van Kan, J. A., & Blanco‐Ulate, B. (2019). Grey mould of strawberry, a devastating disease caused by the ubiquitous necrotrophic fungal pathogen Botrytis cinerea. Molecular Plant Pathology, 20(6), 877–892. https://doi.org/10.1111/mpp.12794
Philippot, L., Chenu, C., Kappler, A., Rillig, M.C., & Fierer, N. (2024). The interplay between microbial communities and soil properties. Nature Reviews Microbiology, 22(4), 226–239. https://doi.org/10.1038/s41579-023-00980-5
Rahman, M.U., Liu, X., Wang, X., & Fan, B. (2024). Grapevine gray mold disease: Infection, defense and management. Horticulture Research, 11(9), uhae182.https://doi.org/10.1093/hr/uhae182
Roatti, B., Perazzolli, M., Gessler, C., & Pertot, I. (2013). Abiotic stresses affect Trichoderma harzianum T39-induced resistance to downy mildew in grapevine. Phytopathology, 103(12), 1227–1234. https://doi.org/10.1094/PHYTO-02-13-0040-R
Rombach, H., Alon, H., Shapiro, O.H., Elad, Y., & Kleiman, M. (2022). Elucidating the effect of tomato leaf surface microstructure on Botrytis cinerea using synthetic systems. Frontiers Plant Science, 13, 1023502. https://doi.org/10.3389/fpls.2022.1023502
Saito, S., & Xiao, C. L. (2018). Fungicide resistance in Botrytis cinerea populations in California and its influence on control of gray mold on stored mandarin fruit. Plant Disease, 102(12), 2545–2549. https://doi.org/10.1094/PDIS-05-18-0766-RE
Saito, S., Michailides, T.J., & Xiao, C.L. (2019). Fungicide-resistant phenotypes in Botrytis cinerea populations and their impact on control of gray mold on stored table grapes in California. European Journal Plant Pathology, 154(2), 203–213. https://doi.org/10.1007/s10658-018-01649-z
Shen, F., Wu, W., Han, X., Wang, J., Li, Y., & Liu, D. (2021). Study on the occurrence law and green control of grape gray mold from the perspective of ecological balance. Bioengineered, 12(1), 779-790. https://doi.org/10.1080/21655979.2021.1888578
SPSS, (2023). SPSS v.29.0 for Microsoft Windows, SPSS Inc., Chicago, IL, USA.
Stange, P., Kersting, J., Sivaprakasam Padmanaban, P.B., Schnitzler, J.P., Rosenkranz, M., Karl, T., & Benz, J.P. (2024). The decision for or against mycoparasitic attack by Trichoderma spp. is taken already at a distance in a prey-specific manner and benefits plant-beneficial interactions. Fungal Biology Biotechnology, 11(1), 14. https://doi.org/10.1186/s40694-024-00183-4
Steel, C. C., Blackman, J. W., & Schmidtke, L. M. (2013). Grapevine bunch rots: impacts on wine composition, quality, and potential procedures for the removal of wine faults. Journal of Agricultural and Food Chemistry, 61(22), 5189-5206. https://doi.org/10.1021/jf400641r
Sui, Y., Ning, X., Huang, K., Huang, P., Wang, Z., Yan, C., ... & Wang, Z. (2026). Deciphering the molecular mechanisms of kiwifruit defense response induced by Trichoderma harzianum against Botrytis cinerea. Postharvest Biology Technology, 232, 113959. https://doi.org/10.1016/j.postharvbio.2025.113959
Tyśkiewicz, R., Nowak, A., Ozimek, E., & Jaroszuk-Ściseł, J. (2022). Trichoderma: The current status of its application in agriculture for the biocontrol of fungal phytopathogens and stimulation of plant growth. International Journal Molecular Sciences, 23(4), 2329. https://doi.org/10.3390/ijms23042329
Waheed, Z., Anwar, W., Anjum, T., Abbas, M.T., Akhter, A., Hashem, A., ... & Abd-Allah, E.F. (2024). Pyrolysed maize feedstock utilization in combination with Trichoderma viride against Macrophomina phaseolina. Scientific Reports, 14(1), 19762. https://doi.org/10.1038/s41598-024-70975-6.
Vinale, F., Sivasithamparam, K., Ghisalberti, E.L., Woo, S.L., Nigro, M., Marra, R., ... & Lorito, M. (2014). Trichoderma secondary metabolites active on plants and fungal pathogens. Open Mycol. J, 8(1), 127–139.
Yang, Y., Zhao, M., Li, G., Wang, Y., Shen, Q., Yang, J., ... & Ahmed, W. (2025). Molecular tactics of biocontrol fungi to hack plant immunity for successful host colonization—A focus on Trichoderma fungi. Microorganisms, 13(6), 1251. https://doi.org/10.3390/microorganisms13061251
Zúñiga, C.C.A., Chan-Cupul, W., Rangel, J. C.S., Buenrostro-Nava, M.T., & Manzo-Sanchez, G. (2024). Actividad inhibitoria in vitro de Trichoderma spp. sobre Botrytis cinerea, agente causal del moho gris en frambuesa: in vitro ınhibitory activity of Trichoderma spp. on Botrytis cinerea, causal agent of gray mold in raspberry. Biotecnología y Sustentabilidad, 9(1), 1–11. https://doi.org/10.57737/gd2f7y77

Details

Primary Language

English

Subjects

Phytopathology

Journal Section

Research Article

Authors

Yunus Korkom ^*
0000-0001-5859-9026
Türkiye

Emrecan Altıntaş
0009-0007-6062-8755
Türkiye

Publication Date

December 26, 2025

Submission Date

October 26, 2025

Acceptance Date

December 5, 2025

Published in Issue

Year 2025 Volume: 9 Number: 4

DOI

https://doi.org/10.31015/2025.4.18

IZ

https://izlik.org/JA72EG67FW

APA

Korkom, Y., & Altıntaş, E. (2025). Effectiveness of Native Trichoderma Isolates Against Gray Mold in Vitis vinifera. International Journal of Agriculture Environment and Food Sciences, 9(4), 1162-1168. https://doi.org/10.31015/2025.4.18

AMA

1.Korkom Y, Altıntaş E. Effectiveness of Native Trichoderma Isolates Against Gray Mold in Vitis vinifera. int. j. agric. environ. food sci. 2025;9(4):1162-1168. doi:10.31015/2025.4.18

Chicago

Korkom, Yunus, and Emrecan Altıntaş. 2025. “Effectiveness of Native Trichoderma Isolates Against Gray Mold in Vitis Vinifera”. International Journal of Agriculture Environment and Food Sciences 9 (4): 1162-68. https://doi.org/10.31015/2025.4.18.

EndNote

Korkom Y, Altıntaş E (December 1, 2025) Effectiveness of Native Trichoderma Isolates Against Gray Mold in Vitis vinifera. International Journal of Agriculture Environment and Food Sciences 9 4 1162–1168.

IEEE

[1]Y. Korkom and E. Altıntaş, “Effectiveness of Native Trichoderma Isolates Against Gray Mold in Vitis vinifera”, int. j. agric. environ. food sci., vol. 9, no. 4, pp. 1162–1168, Dec. 2025, doi: 10.31015/2025.4.18.

ISNAD

Korkom, Yunus - Altıntaş, Emrecan. “Effectiveness of Native Trichoderma Isolates Against Gray Mold in Vitis Vinifera”. International Journal of Agriculture Environment and Food Sciences 9/4 (December 1, 2025): 1162-1168. https://doi.org/10.31015/2025.4.18.

JAMA

1.Korkom Y, Altıntaş E. Effectiveness of Native Trichoderma Isolates Against Gray Mold in Vitis vinifera. int. j. agric. environ. food sci. 2025;9:1162–1168.

MLA

Korkom, Yunus, and Emrecan Altıntaş. “Effectiveness of Native Trichoderma Isolates Against Gray Mold in Vitis Vinifera”. International Journal of Agriculture Environment and Food Sciences, vol. 9, no. 4, Dec. 2025, pp. 1162-8, doi:10.31015/2025.4.18.

Vancouver

1.Yunus Korkom, Emrecan Altıntaş. Effectiveness of Native Trichoderma Isolates Against Gray Mold in Vitis vinifera. int. j. agric. environ. food sci. 2025 Dec. 1;9(4):1162-8. doi:10.31015/2025.4.18

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Effectiveness of Native Trichoderma Isolates Against Gray Mold in Vitis vinifera

Effectiveness of Native Trichoderma Isolates Against Gray Mold in Vitis vinifera

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