Sequence data characterization and development of DNA markers for sesame (Sesamum indicum L.)
Abstract
New
DNA sequencing techniques enable researchers obtain large quantity of sequence
information, which are deposited in digital storage or could be further mined
for other purposes. Sesame (Sesamum
indicum L.) is one of very important oilseed crops, its seed oil contains
many antioxidant properties making sesame the queen of oil crops. Today,
molecular plant breeding technology is indispensable for plant breeders and seed
producers. Compared to other seed oil crops the available number of
microsatellite markers in sesame is still not sufficient enough for the
development of polymorphic markers for breeding and genetic studies. Thus, new
approaches or resources are needed for development of microsatellite markers
for sesame. In the present study, we utilized a total of 45099 transcribed genomic
DNA sequences/expressed sequence tags and mined these sequences for studying frequency
of microsatellite motifs, ranging from di- to hexa-nucleotides with four to ten
tandem repeats, and repeat numbers greater than 10. Using mined transcribed
data, 42 putative microsatellite markers were developed and characterized at
the sequence level. However, we did not confirm these markers and have no
information about the level of their polymorphisms in sesame in vitro. We discussed the biological
meaning of the motif lengths and repeat numbers in the sesame genome.
Keywords
Microsatellite,Motif length,Repeating motif,Primer pairs,Tandem repeats
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