In vitro investigation of antimicrobial, enzyme inhibitory and free radical scavenging activities of Inula salicina L.

Aybeniz Yıldırım; Ali Şen; Mayram Tuysuz; Ayşe Seher Birteksöz Tan; İsmail Şenkardeş; Leyla Bitiş

doi:10.31015/jaefs.2022.3.7

EN

In vitro investigation of antimicrobial, enzyme inhibitory and free radical scavenging activities of Inula salicina L.

Abstract

In this study, in vitro biological activities and total phenol/flavonoid contents of methanol extract (ISM) and its hexane (ISH), chloroform (ISC), ethyl acetate (ISEA) and aqueous methanol (ISAM) fractions obtained from aerial parts of Inula salicina were investigated. ISEA showed the highest antioxidant activity against DPPH and ABTS radicals with an IC50 value of 0.014 mg ml-1for both assays. ISEA exhibited a good anti-inflammatory activity with an IC50 value of 0.060 mg ml-1. ISEA was found to exhibit a moderate level of antidiabetic activity against α amylase enzyme with an IC50 value of 0.290 mg ml-1. ISEA and ISM presented low and moderate inhibitory activity against acetylcholinesterase and butyrylcholinesterase enzymes with IC50 values of 0.577 and 0.279 mg ml-1, respectively. ISC with MIC values of 78 and 156 µg ml-1 displayed a significant antimicrobial activity against Staphylococcus aureus and S. epidermidis, respectively. Almost all extracts had moderate effect against Candida species. The highest total phenolic and flavonoid contents were determined in ISEA with 574.8 mg GAE (gallic acid equivalent) g-1 extract and 30.48 mg QE (quercetin equivalent) g-1 extract, respectively. These results showed that ISEA had a good antioxidant and anti-inflammatory activity with moderate α-amylase and butyrylcholinesterase inhibitory activity. Also, ISC exhibited a significant antimicrobial activity against Staphylococcus species.

Keywords

Asteraceae, Biological activity, Extracts, Fractions, Inula salicina

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Details

Primary Language

English

Subjects

Botany

Journal Section

Research Article

Authors

Aybeniz Yıldırım
0000-0002-5801-4726
Türkiye

Ali Şen ^*
0000-0002-2144-5741
Türkiye

Mayram Tuysuz
0000-0003-0823-631X
Türkiye

Ayşe Seher Birteksöz Tan This is me
0000-0001-8767-710X
Türkiye

İsmail Şenkardeş
0000-0002-2656-0319
Türkiye

Leyla Bitiş
0000-0003-1167-6666
Türkiye

Publication Date

September 23, 2022

Submission Date

May 12, 2021

Acceptance Date

July 14, 2022

Published in Issue

Year 2022 Volume: 6 Number: 3

DOI

https://doi.org/10.31015/jaefs.2022.3.7

IZ

https://izlik.org/JA52AN98JH

APA

Yıldırım, A., Şen, A., Tuysuz, M., Birteksöz Tan, A. S., Şenkardeş, İ., & Bitiş, L. (2022). In vitro investigation of antimicrobial, enzyme inhibitory and free radical scavenging activities of Inula salicina L. International Journal of Agriculture Environment and Food Sciences, 6(3), 389-395. https://doi.org/10.31015/jaefs.2022.3.7

AMA

1.Yıldırım A, Şen A, Tuysuz M, Birteksöz Tan AS, Şenkardeş İ, Bitiş L. In vitro investigation of antimicrobial, enzyme inhibitory and free radical scavenging activities of Inula salicina L. int. j. agric. environ. food sci. 2022;6(3):389-395. doi:10.31015/jaefs.2022.3.7

Chicago

Yıldırım, Aybeniz, Ali Şen, Mayram Tuysuz, Ayşe Seher Birteksöz Tan, İsmail Şenkardeş, and Leyla Bitiş. 2022. “In Vitro Investigation of Antimicrobial, Enzyme Inhibitory and Free Radical Scavenging Activities of Inula Salicina L”. International Journal of Agriculture Environment and Food Sciences 6 (3): 389-95. https://doi.org/10.31015/jaefs.2022.3.7.

EndNote

Yıldırım A, Şen A, Tuysuz M, Birteksöz Tan AS, Şenkardeş İ, Bitiş L (September 1, 2022) In vitro investigation of antimicrobial, enzyme inhibitory and free radical scavenging activities of Inula salicina L. International Journal of Agriculture Environment and Food Sciences 6 3 389–395.

IEEE

[1]A. Yıldırım, A. Şen, M. Tuysuz, A. S. Birteksöz Tan, İ. Şenkardeş, and L. Bitiş, “In vitro investigation of antimicrobial, enzyme inhibitory and free radical scavenging activities of Inula salicina L”., int. j. agric. environ. food sci., vol. 6, no. 3, pp. 389–395, Sept. 2022, doi: 10.31015/jaefs.2022.3.7.

ISNAD

Yıldırım, Aybeniz - Şen, Ali - Tuysuz, Mayram - Birteksöz Tan, Ayşe Seher - Şenkardeş, İsmail - Bitiş, Leyla. “In Vitro Investigation of Antimicrobial, Enzyme Inhibitory and Free Radical Scavenging Activities of Inula Salicina L”. International Journal of Agriculture Environment and Food Sciences 6/3 (September 1, 2022): 389-395. https://doi.org/10.31015/jaefs.2022.3.7.

JAMA

1.Yıldırım A, Şen A, Tuysuz M, Birteksöz Tan AS, Şenkardeş İ, Bitiş L. In vitro investigation of antimicrobial, enzyme inhibitory and free radical scavenging activities of Inula salicina L. int. j. agric. environ. food sci. 2022;6:389–395.

MLA

Yıldırım, Aybeniz, et al. “In Vitro Investigation of Antimicrobial, Enzyme Inhibitory and Free Radical Scavenging Activities of Inula Salicina L”. International Journal of Agriculture Environment and Food Sciences, vol. 6, no. 3, Sept. 2022, pp. 389-95, doi:10.31015/jaefs.2022.3.7.

Vancouver

1.Aybeniz Yıldırım, Ali Şen, Mayram Tuysuz, Ayşe Seher Birteksöz Tan, İsmail Şenkardeş, Leyla Bitiş. In vitro investigation of antimicrobial, enzyme inhibitory and free radical scavenging activities of Inula salicina L. int. j. agric. environ. food sci. 2022 Sep. 1;6(3):389-95. doi:10.31015/jaefs.2022.3.7

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Abstract

In vitro investigation of antimicrobial, enzyme inhibitory and free radical scavenging activities of Inula salicina L.

Abstract

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Cited By

Investigation of Some Biological Activities of Inula graveolens (L.) Desf Species from Turkey

Inula germanica as a Medicinal Plant: Phenolic Compounds, Antioxidant Capacity, and Antimicrobial Effects

Impact of Climate Change on the Composition and Bioactivity of Inula viscosa Essential Oils: In Vitro, In Silico, and ADME Analysis

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