Determination of effective surface sterilization protocol in in vitro tissue culture for Giant Snowdrop (Galanthus elwesii Hook) bulbs

Yıl 2023, Cilt: 7 Sayı: 2, 345 - 348, 29.06.2023

Çağlar Kaya Tolga Sarıyer Esra Şahin

https://doi.org/10.31015/jaefs.2023.2.12

Öz

Giant Snowdrop (Galanthus elwesii Hook) is a species of snowdrop belonging to the Amaryllidaceae family. In this study, the deformation rates of the bulbs was calculated as % (percent). For sterilization G. elwesii bulbs were sterilized at different concentrations (1, 3, 5, 7, 9, 11 and 13 %) of sodium hypochlorite (NaOCl) for 5, 10, 15, 20, 25 minutes. The bulbs were rinsed with sterile distilled water 3 times for 5 minutes and then transferred to the MS medium. Contamination rates in MS nutrient medium were determined as percent (%) after 7 days. Deformation was not observed in the bulbs sterilized with 1 and 3% sodium hypochlorite solution however 100% contamination was detected. The most effective surface sterilization was obtained by soaking in 9-11% sodium hypochlorite solution for 5 and 10 minutes. As the sodium hypochlorite concentration and the application time increased, the surface sterilization of the bulbs increased, but the deformation rate of the scaly leaves of the bulbs increased due to this increase.

Anahtar Kelimeler

Giant Snowdrop, Bulb, Sterilization, Contamination, Deformation

Kaynakça

• Arpa, N.Y. (2012). The role and importance of protected areas in the conservation of biodiversity. Biological Diversity Symposium Proceedings, 103-107.
• Baktır, İ., Tezcan, Ö. and Kaynakcı, Z. (1997). Geofitlerin çevre değerleri açısından önemi. Akdeniz Üniversitesi Ziraat Fakültesi Dergisi, 10(1), 408-413.
• Farooq, I., Banday, N., Nazki, I., Malik, A. A., Khan, F., Mir, M., Yaseen, T. (2022). In vitro sterilization of Lilium LA Hybrids “Indian Summerset” and “Nashville” as influenced by different sterilant combinations. The Pharma Innovation Journal, 11(11), 1795-1798.
• Girmen, M. and K. Zimmer. (1988). In vitro-kultur von Galanthus elwesii 1. sterilisation, regeneration, phytohormone. Gartenbauwissenschaft, 53(1), 26-29.
• Gochhayat, A. A., Beura, S., Subudhi, E. (2017). Effect of surface sterilization time and plant bioregulators for callus formation in hybrid Lilium Cv. Tresor. Biosci., Biotech. Res. Asia, 14(2), 709-713. https://doi.org/10.13005/bbra/2498
• Groenewald, E.G., A. Koeleman, and D.C. Wessels. (1975). Callus formation and plant regeneration from seed tissue of aloe pretoriensis. Z. Pflanzenphysiol. 75, 270-272. https://doi.org/10.1016/S0044-328X(75)80089-4
• Kone, T., Kone, M., Silva, J. A. T., Kone, D., Kouadio, Y. J. (2011). Effect of substrate type and bulb size on in vivo production of seedlings in three cultivars of plantain (Musa spp.). The African Journal of Plant Science and Biotechnology, 5(1), 50-55.
• Leveque, C., Mounolou, J.C. (2003). Biodiversity, John Wiley & Sons Ltd.
• Murashige T., Skoog F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, 15, 473–497.
• Staikidou, I., Selby, C. and Watson, S. (2008). Efficient surface sterilization of snowdrop bulb explants with ppm. in x international symposium on flower bulbs and herbaceous perennials, 886, 259-265. https://doi.org/10.17660/ActaHortic.2011.886.36
• Tıpırdamaz, R., Ellialtıoğlu, Ş. and Çakırlar H. (1999). Propagation of snowdrop (Galanthus ikariae baker.) by tissue culture: the effect of explant type, media ph and carbohydrate source on bulb formation. Tr. J. of Agriculture and Forestry, 4, 823-830.